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DIAGNOSA DEMAM TIFOID DISERTAI KONDISI KADAR LEUKOSIT PASIEN DI RUMAH SAKIT ISLAM SAKINAH MOJOKERTO FARIHATUN NAFIAH; ROMYUN ALVY KHOIRIYAH; MISBAKHUL MUNIR
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 1, No 1 (2017): Klorofil: Jurnal Ilmu Biologi dan Terapan
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v1i1.1231

The first step of tifoid fever diagnosis is through the test of leukocyte levels using hemadializer. Leukocytes are a major component of body immunity, especially in the fight against infection. Early symptoms of typhoid fever resemble fever in general, so it needs to be continued with more specific test one tubex test as applied to clinical pathology laboratory Islamic Hospital Sakinah Mojokerto. The results obtained are leukocyte levels in patients divided into three categories, below normal limits, normal, and above normal levels. So the case of typhoid fever becomes very interesting to continue to be studied and presented mechanism of the infection in detail.

Comparison of DNA Isolation Results with Simple Methods and Kits in Samples of Psidium guajava Leaves Yuanita Rachmawati; Romyun Alvy Khoiriyah
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

DNA isolation is one of a series of methods that must be carried out on the basic techniques of Molecular Biology Analysis. Especially PCR-based molecular marking techniques. Many ways are done in DNA isolation. This study discusses the comparison of the results of DNA isolation using two methods. Simple DNA isolation methods and using Kit. Samples of Psidium guajava leaves used were taken from 15 different locations used. In general, DNA isolation methods include three steps, namely destruction, precipitation, and purification. Simple DNA isolation is done with detergents, alcohol groups, which are commonly available in the laboratory. Methods of DNA isolation with KIT are carried out according to the Promega Universal Wizard KIT protocol. The comparison results are seen from spectrophotometric absorption Å230 nm, Å260 nm, Å280 nm, Å320 nm, ratio Å260/Å230, ratio Å260/Å280 to see DNA purity, protein concentration before purification step, and DNA concentration produced. The results showed that there were no statistically significant differences in the results of DNA isolate spectrophotometry. However, the use of KIT with modified protocols is more recommended if researchers want to carry out DNA analysis more precisely and accurately. Keywords: DNA isolation, spectrophotometry, DNA concentration and purity

DIAGNOSA DEMAM TIFOID DISERTAI KONDISI KADAR LEUKOSIT PASIEN DI RUMAH SAKIT ISLAM SAKINAH MOJOKERTO FARIHATUN NAFIAH; ROMYUN ALVY KHOIRIYAH; MISBAKHUL MUNIR
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 1, No 1 (2017): Klorofil: Jurnal Ilmu Biologi dan Terapan
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v1i1.1231

The first step of tifoid fever diagnosis is through the test of leukocyte levels using hemadializer. Leukocytes are a major component of body immunity, especially in the fight against infection. Early symptoms of typhoid fever resemble fever in general, so it needs to be continued with more specific test one tubex test as applied to clinical pathology laboratory Islamic Hospital Sakinah Mojokerto. The results obtained are leukocyte levels in patients divided into three categories, below normal limits, normal, and above normal levels. So the case of typhoid fever becomes very interesting to continue to be studied and presented mechanism of the infection in detail.

Religiusitas, Self-Disclosure dengan Penyesuaian Diri Santri Baru Faizah Oktaviani; Moh. Sholeh; Romyun Alvy Khoiriyah
Jurnal Penelitian Psikologi Vol. 14 No. 1 (2023): Jurnal Penelitian Psikologi
Publisher : Program Studi Psikologi Fakultas Psikologi dan Kesehatan Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/jpp.v14i1.911

Santri baru membutuhkan kemampuan penyesuaian diri ketika bergabung dengan pondok pesantren. Penelitian ini bertujuan untuk mengetahui hubungan antara religiusitas dan keterbukaan diri dengan penyesuaian diri pada santri baru. Penelitian kuantitatif korelasional ini melibatkan 127 santri baru putri kelas VII SMP. Teknik analisis data menggunakan regresi linier berganda. Hasil penelitian menunjukkan bahwa terdapat hubungan yang signifikan antara religiusitas dan keterbukaan diri dengan penyesuaian diri. Religiusitas dan keterbukaan diri merupakan dua variabel yang membentuk penyesuaian diri. Tingkat religiusitas yang baik serta keterbukaan diri yang dimiliki santri mampu memberikan kontribusi terhadap kemampuan santri dalam menyesuaikan diri selama masa-masa awal di pondok pesantren

IDENTIFIKASI SENYAWA METABOLIT SEKUNDER PADA EKSTRAK METHANOL BATANG KEMANGI (Ocimum bacilicum L): IDENTIFICATION OF SECONDARY METABOLYTE IN METHANOL EXTRACT OF BASIL STEMS (Ocimum bacilicum L) Funsu Andiarna; Mei Lina Fitri Kumalasari; Esti Tyastirin; Eko Teguh Pribadi; Romyun Alvy Khoiriyah; Sarita Oktorina
GEMA KESEHATAN Vol. 15 No. 2 (2023): Desember 2023
Publisher : POLTEKKES KEMENKES JAYAPURA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47539/gk.v15i2.420

Tanaman kemangi (Ocimum bacilicum L) adalah salah satu tanaman obat tradisional yang paling banyak dikembangkan di negara Indonesia. Tanaman kemangi memiliki banyak manfaat seperti mengobati demam, diare, batuk, pusing, mual, dan lain sebagainya. Tujuan dari penelitian ini untuk mengidentifikasi senyawa metabolit sekunder pada batang tanaman kemangi. Ekstrak kemangi menggunakan pelarut metanol 96%. Batang daun kemangi dilakukan proses penyarian dengan metode maserasi. Ekstrak kemangi selanjutnya dilakukan uji fitokimia untuk mengetahui kandungan senyawa metabolit sekunder. Hasil dari penelitian ini menunjukkan bahwa pada batang tanaman kemangi terdapat kandungan senyawa metabolit sekunder yaitu flavonoid, tannin, alkaloid dan saponin. Hal ini dapat menjelaskan bahwa ekstrak kemangi memiliki potensi yang kuat dalam aktivitas antioksidan yang memiliki peran penting dalam pemanfaatan obat tradisional. Senyawa flavonoid paling umum dijadikan sebagai antioksidan. Selain itu beberapa jenis senyawa alkaloid, tanin dan saponin juga mempunyai efek antioksidan. Kata Kunci: Kemangi, Metanol, Ocimum bacilicum L, Senyawa metabolit sekunder The basil plant (Ocimum bacilicum L) is one of Indonesia's most developed traditional medicinal plants. Basil plants have many benefits, such as treating fever, diarrhea, cough, dizziness, nausea, etc. The study aims to identify secondary metabolite compounds in the stem of basil plants. Basil extract uses 96% methanol solvent. Stems of basil leaves carried out the process of expulsion by maceration method. The phytochemical test was conducted through basil extraction to determine the secondary content of metabolite compounds. The results of this research show that the stem of the basil plant is a secondary metabolite compound, namely flavonoids, alkaloids, tannins, and saponins. It can explain that basil extract has a strong potential for antioxidant activity that has an essential role in traditional medicine. Keywords: Methanol, Ocimum bacilicum, Secondary metabolites compounds, Stem basil

Evaluation of Ashitaba (Angelica keiskei) Crackers Formulations as α-Glucosidase Enzyme Inhibitors Khoiriyah, Romyun Alvy; Marliyati, Sri Anna; Ekayanti, Ikeu; Handharyani, Ekowati
Media Gizi Indonesia Vol. 19 No. 2 (2024): MEDIA GIZI INDONESIA (NATIONAL NUTRITION JOURNAL)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mgi.v19i2.198-205

One of the global health issues is diabetes mellitus, characterized by elevated blood glucose levels. The absorption of glucose in the body occurs through the digestion of carbohydrates by the enzyme α-glucosidase, which is responsible for hydrolyzing carbohydrates into sugar. Ashitaba (Angelica keiskei) is a herbal plant from Japan and has long been utilized in traditional medicine for its various health benefits and preventive properties against multiple diseases. This plant has also been cultivated in Indonesia, including Trawas Mojokerto, East Java. This study aims to develop ashitaba crackers and evaluate their potential α-glucosidase inhibitory activity to develop safe and effective natural products to assist in managing diabetes more efficiently. This research was conducted from July to September 2022. Two formulations of fresh ashitaba leaves were used (12% CAST 1 and 18% CAST 2 of 100 g of flour). Based on the results of this research, chemical composition tests showed that the carbohydrate and dietary fiber content in CAST 1 were higher than those in CAST 2. Protein, lipid, water, and ash content in CAST 2 had higher values than in CAST 1. Flavonoid content and inhibitory activity test results of ashitaba crackers showed significant differences between the two formulation groups regarding flavonoid content and percentage inhibition against the α-glucosidase enzyme. Both flavonoid content and inhibitory activity in CAST 2 were higher than in CAST 1. Ashitaba crackers with the best formulation in this study, based on flavonoid levels and their ability to inhibit α-glucosidase enzyme, were identified as CAST 2.

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