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All Journal SEL Jurnal Penelitian Kesehatan JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) JURNAL ANALIS LABORATORIUM MEDIK Journal of Parasite Science Indonesian Journal of Global Health research Anakes: Jurnal Ilmiah Analis Kesehatan Jurnal Farmasetis
Dewi Saroh
Sekolah Tinggi Ilmu Kesehatan Nasional
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Detection of Gene Alg8 and Alg44 in Clinical Isolates Pseudomonas Aeruginosa using Plymerase Chain Reaction Method Didik Wahyudi; Wimpy Wimpy; Dewi Saroh
Indonesian Journal of Global Health Research Vol 4 No 4 (2022): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.419 KB) | DOI: 10.37287/ijghr.v4i4.1292

Abstract

The alg8 and alg44 genes are one of the genes that control alginate production in Pseudomonas aeruginosa bacteria, these genes are one of the main virulence factors causing chronic infections in the human body. Pseudomonas aeruginosa is a bacterium that causes infections in several cases in various parts of the body. The purpose of this study was to detect the presence of alg8 and alg44 genes in several isolates of Pseudomonas aeruginosa from several clinical samples (urine, sputum, and pus) using the Polymerase Chains Reaction method. The study was initiated by characterizing and purification of 6 isolates of Pseudomonas aeruginosa from urine, sputum, and pus samples (2 isolates each), identification of isolates was carried out by biochemical tests. Bacterial DNA isolation was carried out using the DNeasy Blood and Tissue Kit, the results of the isolation were tested by electrophoresis. Six samples of Pseudomonas aeruginosa DNA were tested for the presence of alg8 and alg44 genes by PCR method. The primary design was carried out using the website https://www.ncbi.nlm.nih.gov. The alg8 gene as a whole consists of 1214 nitrogenous bases, the primer used produces an amplicon of 882 bp (72.6%), alg44 gene consists of 818bp, the primer used amplifies 316 bp (36%). alg8 and alg44 genes were found in all isolates of Pseudomonas aeruginosa.
Gambaran Kadar Timbal pada Pekerja Pewarna Batik di Laweyan Purwati Purwati; Tri Harningsih; Dewi Saroh
Jurnal Farmasetis Vol 12 No 2 (2023): Jurnal Farmasetis: Mei 2023
Publisher : LPPM Sekolah Tinggi Ilmu Kesehatan Kendal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32583/far.v12i2.1090

Abstract

Kampung Batik Laweyan merupakan suatu kawasan permukiman yang unik, spesifik, dan bersejarah, yaitu menjadi kampung yang terkenal dengan kerajinan batik. Proses membatik dilakukan melalui beberapa tahap, yaitu tahap persiapan, tahap pemolaan, pemalaman, selanjutnya pewarnaan celup, pelorodan atau penghilangan lilin batik dan yang terakhir adalah finishing. Proses perwarnaan  batik menggunakan  pewarna alami dan pewarna sintetik. Kandungan logam berat yaitu timbal pada pewarna batik sintetik memberikan dampak resiko kesehatan bagi para pekerja yang terpapar. Tujuan penelitian ini untuk mengetahui gambaran kadar timbal pada pekerja pewarna batik di Laweyan. Penelitian dilakukan secara deskriptif, dengan jumlah responden sebanyak 21 sampel. Sampel darah diambil sebanyak 3 ml menggunakan vacuum tube EDTA. Selanjutnya sampel dimasukkan dalam ice box yang sudah dilengkapi ice gel untuk dibawa ke Laboratorium Hematologi STIKES Nasional guna dilakukan pemeriksaan profil hematologi dengan menggunakan hematology analyzer. Sampel darah selanjutnya dikirim ke Balai Laboratorium Kesehatan dan Kalibrasi Yogyakarta untuk pemeriksaan kadar timbal menggunakan spektrofotometer serapan atom. Pembacaan hasil diperoleh sebanyak 6 sampel memiliki kadar timbal lebih dari  10 µL/dL; 10 sampel memiliki kadar timbal  kisaran 1-10  µL/dL dan 5 sampel memiliki kadar timbal dibawah 1 µL/dL. Kadar timbal dalam darah melebihi 10 µL/dL terindikasi adanya kemungkinan keracunan timbal.
Prevalence and Intensity of the Infection by Endoparasite Ascaridia galli in Broilers (Gallus gallus domesticus) in Slaughterhouses in Sukoharjo Dwi Haryatmi; Dewi Saroh; Adhi Kumoro Setyo
Journal of Parasite Science Vol. 10 No. 1 (2026): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v10i1.79146

Abstract

Ascariasis often occurs in poultry caused by the worm Ascaridia galli, a nematode worm from the same family as the worm Ascaris lumbricoides. Helminthiasis due to infection by Ascaridia galli often occurs in chickens, which becomes an obstacle causing failure in chicken cultivation. Infection by the worm Ascaridia galli often causes reduced growth rate and weight loss. This study aimed to determine the prevalence and intensity of the infection by Ascaridia galli in slaughterhouses in the Sukoharjo regency. The research was carried out using experimental methods, which took the worm Ascaridia galii out of the intestine of the chickens that had just been slaughtered. The samples were collected from the Animal Slaughterhouse (RPH ~Ind.) in Grogol of Sukoharjo of Central Java, totaling 49 intestines. The results were that of 49 chickens’ intestines examined, 1/49 were found positive for the worm A. galli, with a prevalence of 2.04% and an intensity of 15 worms per infected chicken intestine. This study also found that 5/49 eggs were positive, with a prevalence of 10.20% and an intensity of one egg per infected chicken intestine.  
Co-Authors Adhi Kumoro Setyo Didik Wahyudi Dwi Haryatmi Meutia Sekarmaharani Nova Pramestuti Noviana Dewi Purwati Purwati Ria Komariah Tri Harningsih Wimpy, W Yaninda Setyanti Ekasari