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All Journal SEL Jurnal Penelitian Kesehatan Klinikal Sains (Jurnal Analis Kesehatan) JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) JURNAL ANALIS LABORATORIUM MEDIK Indonesian Journal of Global Health research Anakes: Jurnal Ilmiah Analis Kesehatan Jurnal Farmasetis Jurnal Kesehatan Masyarakat
Dewi Saroh
Sekolah Tinggi Ilmu Kesehatan Nasional
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Detection of Gene Alg8 and Alg44 in Clinical Isolates Pseudomonas Aeruginosa using Plymerase Chain Reaction Method Didik Wahyudi; Wimpy Wimpy; Dewi Saroh
Indonesian Journal of Global Health Research Vol 4 No 4 (2022): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.419 KB) | DOI: 10.37287/ijghr.v4i4.1292

Abstract

The alg8 and alg44 genes are one of the genes that control alginate production in Pseudomonas aeruginosa bacteria, these genes are one of the main virulence factors causing chronic infections in the human body. Pseudomonas aeruginosa is a bacterium that causes infections in several cases in various parts of the body. The purpose of this study was to detect the presence of alg8 and alg44 genes in several isolates of Pseudomonas aeruginosa from several clinical samples (urine, sputum, and pus) using the Polymerase Chains Reaction method. The study was initiated by characterizing and purification of 6 isolates of Pseudomonas aeruginosa from urine, sputum, and pus samples (2 isolates each), identification of isolates was carried out by biochemical tests. Bacterial DNA isolation was carried out using the DNeasy Blood and Tissue Kit, the results of the isolation were tested by electrophoresis. Six samples of Pseudomonas aeruginosa DNA were tested for the presence of alg8 and alg44 genes by PCR method. The primary design was carried out using the website https://www.ncbi.nlm.nih.gov. The alg8 gene as a whole consists of 1214 nitrogenous bases, the primer used produces an amplicon of 882 bp (72.6%), alg44 gene consists of 818bp, the primer used amplifies 316 bp (36%). alg8 and alg44 genes were found in all isolates of Pseudomonas aeruginosa.
Gambaran Kadar Timbal pada Pekerja Pewarna Batik di Laweyan Purwati Purwati; Tri Harningsih; Dewi Saroh
Jurnal Farmasetis Vol 12 No 2 (2023): Jurnal Farmasetis: Mei 2023
Publisher : LPPM Sekolah Tinggi Ilmu Kesehatan Kendal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32583/far.v12i2.1090

Abstract

Kampung Batik Laweyan merupakan suatu kawasan permukiman yang unik, spesifik, dan bersejarah, yaitu menjadi kampung yang terkenal dengan kerajinan batik. Proses membatik dilakukan melalui beberapa tahap, yaitu tahap persiapan, tahap pemolaan, pemalaman, selanjutnya pewarnaan celup, pelorodan atau penghilangan lilin batik dan yang terakhir adalah finishing. Proses perwarnaan  batik menggunakan  pewarna alami dan pewarna sintetik. Kandungan logam berat yaitu timbal pada pewarna batik sintetik memberikan dampak resiko kesehatan bagi para pekerja yang terpapar. Tujuan penelitian ini untuk mengetahui gambaran kadar timbal pada pekerja pewarna batik di Laweyan. Penelitian dilakukan secara deskriptif, dengan jumlah responden sebanyak 21 sampel. Sampel darah diambil sebanyak 3 ml menggunakan vacuum tube EDTA. Selanjutnya sampel dimasukkan dalam ice box yang sudah dilengkapi ice gel untuk dibawa ke Laboratorium Hematologi STIKES Nasional guna dilakukan pemeriksaan profil hematologi dengan menggunakan hematology analyzer. Sampel darah selanjutnya dikirim ke Balai Laboratorium Kesehatan dan Kalibrasi Yogyakarta untuk pemeriksaan kadar timbal menggunakan spektrofotometer serapan atom. Pembacaan hasil diperoleh sebanyak 6 sampel memiliki kadar timbal lebih dari  10 µL/dL; 10 sampel memiliki kadar timbal  kisaran 1-10  µL/dL dan 5 sampel memiliki kadar timbal dibawah 1 µL/dL. Kadar timbal dalam darah melebihi 10 µL/dL terindikasi adanya kemungkinan keracunan timbal.
DETEKSI GEN blaTEM DAN GEN floR PADA ISOLAT KLINIS Salmonella typhi DENGAN METODE DUPLEX PCR Adellya Mirza Putriyani; Dewi Saroh
Jurnal Analis Laboratorium Medik Vol 11 No 1 (2026): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v11i1.6059

Abstract

Latar belakang: Salmonella typhi merupakan bakteri penyebab infeksi demam tifoid. Diagnosis laboratorium bakteri Salmonella thypi dapat dilakukan secara molekuler yang bertujuan untuk mendeteksi gen DNA pada bakteri yang diperiksa. Tujuan: untuk mengetahui ada tidaknya gen blaTEM dan gen floR pada isolat klinis Salmonella typhi yang diperoleh dari RS Universitas Sebelas Maret dan RSUD Dr. Moewardi. Metode: analisis deskriptif. Sampel yang digunakan adalah isolat klinis Salmonella typhi dari pasien demam tifoid di RS Universitas Sebelas Maret dan RSUD Dr. Moewardi. Hasil: penelitian diperoleh bahwa gen blaTEM resisten terhadap antibiotik ampisilin yang terdeteksi pada panjang produk 643 bp dan gen floR resisten terhadap antibiotik kloramfenikol yang terdeteksi pada panjang produk 250 bp pada isolat klinis Salmonella typhi dengan menggunakan metode duplex Polymerase Chain Reaction. Kesimpulan: Hasil penelitian menunjukkan bahwa dari 12 sampel isolat bakteri, ditemukan gen blaTEM (643 bp) dan gen floR (250 bp) pada Salmonella typhi. Dengan metode Duplex PCR, hasil ini menunjukkan bahwa sampel darah pasien demam tifoid tersebut resisten terhadap antibiotik ampisilin dan kloramfenikol.
DESKRIPSI KADAR TIMBAL DALAM DARAH DAN JUMLAH ERITROSIT PADA PEKERJA BATIK Purwati -; Indah Tri Susilowati; Dewi Saroh; Kartika Pertiwi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 1 (2025): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikalsains.v13i1.5905

Abstract

In the batik industry that uses synthetic dyes as batik dyes, batik industry workers are at risk of exposure to lead contained in synthetic dyes. Continuous exposure to lead can cause health problems and damage erythrocytes, thereby shortening the life of erythrocytes and reducing the number of erythrocytes. This study aims to determine the levels of lead in the blood and the number of erythrocytes in batik industry workers in Banaran Village, Grogol, Sukoharjo. The method used in this study is a descriptive cross-sectional method and a purposive sampling method, as many as 25 respondents who meet the criteria and have filled out the informed consent. The examination data were analyzed using a bivariate test with a significance level used to test the significance of the relationship is <0.05. The results obtained showed that the levels of lead in the blood of 25 respondents were still within the normal threshold according to the CDC, which is <10 µg / dL with lead levels between 0.03 µg / dL-4.896 µg / dL. The results of the erythrocyte count examination showed that 1 (4%) respondents had a lower than normal erythrocyte count and 24 (96%) respondents had a normal erythrocyte count. Lead levels in batik industry workers in Banaran Village, Grogol, Sukoharjo are still within the normal threshold according to the CDC and most batik industry workers have normal erythrocyte counts.
GAMBARAN POLA PEWARISAN MUTASI A3243G (GEN MT-TL1) PADA ANAK DENGAN IBU PENYANDANG DIABETES MELITUS TIPE 2 DEWI SAROH; ASTUTI DWI CAHYANINGRUM
Klinikal Sains : Jurnal Analis Kesehatan Vol. 14 No. 1 (2026): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Type 2 diabetes mellitus is a chronic metabolic disorder with a continuously increasing prevalence and is influenced by genetic factors, including mitochondrial DNA mutations. The A3243G mutation in the MT-TL1 gene is known to exhibit maternal inheritance and is associated with mitochondrial dysfunction that may affect insulin secretion. This study aimed to analyze the inheritance pattern of the A3243G mutation in the MT-TL1 gene among children of mothers with type 2 diabetes mellitus. This study employed an observational design with a cross-sectional approach. The study samples consisted of 9 mothers with type 2 diabetes mellitus and 9 of their children, selected using a quota sampling technique. Venous blood samples were collected for DNA isolation and subsequently analyzed at the molecular level using the Polymerase Chain Reaction–Allele Specific Amplification (PASA) method. The results showed that the A3243G mutation in the MT-TL1 gene was detected in all maternal samples and was also identified in all corresponding child samples, suggesting the presence of heteroplasmy and a possible maternal inheritance pattern. This study has several limitations, including a relatively small sample size and the use of a cross-sectional design, which does not allow for direct causal inference. Therefore, these findings suggest that the A3243G mutation in the MT-TL1 gene may be maternally inherited and can be identified through molecular analysis using the PASA method.
DNA Quality Testing of Isolated Stored Giemsa Blood Smear Samples using Geneius™ Micro gDNA Extraction Kit as An Alternative Sample for Malaria Molecular Examination Dewi Saroh; Dwi Haryatmi; Rendyansah Agung Widodo
Indonesian Journal of Global Health Research Vol 7 No 1 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i1.4844

Abstract

The gold standard for malaria examination is microscopic examination. However, microscopic examination has many shortcomings, namely the difficulty of distinguishing between Plasmodium sp. species when mixed infections occur. Other malaria examinations for screening can be done with RDT. However, it also has shortcomings, namely the minimum number of parasites that can be detected is 100 parasites/ul. Molecular examination is the most sensitive and accurate examination in the diagnosis of malaria. The initial stage of molecular examination is the DNA isolation stage. This DNA isolation is very important in determining the success of molecular examination. Isolation of Plasmodium sp. DNA using blood smear samples is still rarely done and there is no standard procedure for isolating DNA from blood smears. The purpose of this study was to determine whether stored Giemsa blood smears can be used as samples in DNA isolation in malaria cases. The method used in isolation is using the Geneius™ Micro gDNA Kit. The results of the study were that stored Giemsa blood smear samples of Plasmodium vivax for 3 months could be isolated using the Geneius™ Micro gDNA Kit and could be used as DNA templates for amplification with target gene primers.
The Relationship of HBA1C with Mean Platelet Volume (MPV) in Type 2 Diabetes Mellitus Patients Muhammad Sahid; Dewi Saroh
Indonesian Journal of Global Health Research Vol 5 No 4 (2023): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v5i4.2200

Abstract

Diabetes Mellitus (DM) is a chronic disease that occurs due to the failure of the pancreas to produce sufficient insulin. Diabetes Mellitus not only causes death but is also a major cause of blindness, heart disease, and kidney failure. Examination of HbA1c levels in the blood serves to help diagnose diabetes mellitus. A high HbA1c value is used as a measure of the development of diabetes complications. Mean Platelet Volume (MPV) is a marker of platelet function and activation. Platelets in DM patients will synthesize thromboxane in larger quantities than normal platelets. So it is important to check Hba1c and MPV to detect complications. This study aims to determine the relationship between HbA1c and MPV values ​​in type-2 DM patients. The type of research used is observational analysis with a cross-sectional approach. The sampling technique was quota sampling technique, then the results were analyzed using the Spearman correlation test. The results of the Spearman's Rho correlation test in this study showed a significant p-value of 0.100, which means that there is no significant relationship between HbA1c levels and MPV values ​​in patients with type 2 DM.
HUBUNGAN MENOPOUSE DENGAN ANGKA KEJADIAN RHEUMATOID ARTHRITIS Mikhael Dave Christiawan; Dewi Saroh
Jurnal Analis Laboratorium Medik Vol 10 No 1 (2025): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

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Abstract

Rheumatoid arthritis (RA) adalah salah satu penyakit autoimun yang paling sering terjadi di masyarakat. Penyakit ini ditandai dengan peradangan pada lapisan sinovium sendi. Hingga sekarang penyebab dari RA masih belum diketahui, ada yang menyebutkan faktor genetik dan faktor lingkungan dapat meningkatkan risiko penyakit RA Hasil Rheumatoid Factor (RF) ditemukan lebih dari 70% penderita RA. Kebanyakan penyakit RA berlangsung lama/ kronis yaitu sembuh dan kambuh kembali secara berulang-ulang sehingga menyebabkan kerusakan sendi secara menetap. Masalah yang disebabkan oleh penyakit RA tidak hanya berupa keterbatasan yang tampak jelas pada mobilitas dan aktivitas hidup sehari- hari tetapi juga efek sistemik yang tidak jelas yang dapat menimbulkan kegagalan organ.Tujuan penelitian ini untuk mengetahui ada tidaknya hubungan menopouse dengan kejadian Rheumatoid Arthritis. Jenis penelitian ini observasional analitik dengan teknik accidental sampling. Penelitian dilakukan di Laboratorium Klinik Prodia Cepu dengan pemeriksaan RF metode aglutinasi. Penelitian ini menggunakan desain studi cross sectional dengan jumlah sampel 30 responden. Variabel penelitian ini adalah menopouse dan nilai Rheumatoid Factor. Hasil uji statistik dengan SPSS menggunakan Fisher’s exact test didapatkan nilai sig = 0,005. Kesimpulan penelitian ini terdapat hubungan antara menopause disertai nyeri sendi dengan kejadian Rheumatoid Arhtritis.
Gender Differences in Biochemical ExaminationAmong Paint Industry Workers Purwati; Indah Tri Susilowati; Noviana Dewi; Chusnul Chotimah; Ratna Setyaningrum; Tri Harningsih; Dewi Saroh
Jurnal Kesehatan Masyarakat Vol. 21 No. 3 (2026)
Publisher : Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v21i3.30675

Abstract

The production of paint involves the use of volatile organic compounds (VOCs) and heavy metals, which can impact health. Gender differences play a significant role in toxicokinetics and toxicodynamics. Variations in sex hormones, xenobiotic-metabolizing enzymes, and distribution volumes influence the body's response to toxins. This study aims to investigate biochemical parameters—including ALP, AST, ALT, Gamma-GT, total cholesterol, creatinine, cholinesterase, total bilirubin, fasting glucose, N Urea, eLFG, and uric acid—in paint industry workers, stratified by gender. An analytical observational study with a cross-sectional design was conducted on 89 workers engaged in production, research and development, and logistics within a paint manufacturing facility. Demographic data were obtained via questionnaires, and laboratory test results were analyzed. Statistical evaluation was performed using the chi-square test to determine the association between gender and biochemical abnormalities, with an odds ratio (OR), 95% confidence interval (CI), and significance level set at p<0.05. The assessment of paint exposure among workers, based on biochemical test results stratified by gender, demonstrated that gender significantly influences these parameters (p<0.05). The findings of alkaline phosphatase (ALP) (OR: 7.500, 95% CI: 1.146–49.100), total cholesterol (OR: 3.841, 95% CI: 1.142–12.916), and creatinine (OR: 30.179, 95% CI: 7.706–118.193) suggest that exposure to paint substances impacts liver function (as indicated by ALP), total cholesterol, and renal function (creatinine). These outcomes may be attributed to physiological, hormonal, enzymatic, and genetic differences between male and female individuals in their toxicological responses, as supported by biochemical examination results.
The Neurotoxic Impact Of Lead On The Appearance Of Antisocial Behavior In Batik Dye Workers Indah Tri Susilowati; Noviana Dewi; Dewi Saroh
Jurnal Kesehatan Masyarakat Vol. 20 No. 3 (2025)
Publisher : Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v20i3.3666

Abstract

Batik home industry waste not managed with standardized WWTP can lead to environmental pollution. This is caused by the remaining synthetic batik dyes containing heavy metal lead. The remaining dyeing waste is disposed of into the river, a water source in the surrounding environment. Waste containing heavy metals causes a neurotoxic effect on the human body. Furthermore, exposure to heavy metals due to synthetic batik dyes is more at risk for workers who do coloring since they do not use personal protective equipment while working and do not live a clean and healthy lifestyle. One of the behavioral disorders associated with the effects of accumulated lead exposure is an antisocial personality disorder. In this study, blood lead levels were checked for batik dye workers by taking blood samples using a 3 ml purple vacuum tube and placing them in an ice box to be sent to the Yogyakarta Health Laboratory Center (BLK). The blood sample was then wet destroyed, and the Pb levels were read using an AAS tool. Antisocial tendency scale data was measured using STAB (Subtypes of Antisocial Behavior), where the validity test had previously been carried out with the Pearson product-moment of 0.327-0.786 and the reliability test with the Cronbach alpha technique of 0.936. The results of the data analysis showed a correlation between blood lead levels and antisocial disorder tendencies of 0.690, but no correlation was found between blood lead levels and body mass index (BMI)
Co-Authors Adellya Mirza Putriyani ASTUTI DWI CAHYANINGRUM Chusnul Chotimah Didik Wahyudi Dwi Haryatmi Indah Tri Susilowati Kartika Pertiwi Meutia Sekarmaharani Mikhael Dave Christiawan Muhammad Sahid Nova Pramestuti Noviana Dewi Noviana Dewi Purwati Purwati - Purwati Purwati Ratna Setyaningrum Rendyansah Agung Widodo Ria Komariah Tri Harningsih Wimpy, W Yaninda Setyanti Ekasari