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All Journal Indonesian Journal of Biotechnology Jurnal Sains Teknologi dan Lingkungan (JSTL) Abdimasku : Jurnal Pengabdian Masyarakat Prosiding Seminar Nasional Biologi Life Science
R Susanti
Universitas Negeri Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Public Health Social Sciences

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Telaah studi kandungan probiotik pada fermentasi makanan khas di pulau Jawa Novita Kusuma Wardani; R. Susanti; Talitha Widiatningrum
JURNAL SAINS TEKNOLOGI & LINGKUNGAN Vol. 7 No. 1 (2021): JURNAL SAINS TEKNOLOGI & LINGKUNGAN
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v7i1.208

Abstract

The food industry is one of the commodities that always grows and develops in line with the increasing needs and demands of the market. The growth in demand for fermented foods in the market is due in part to the increased interest in healthy food and the awareness of consumers' perceptions that fermentation is a naturally beneficial process. The ferementation process is inseparable from the role of probiotic microorganisms, bioactive compounds, and micronutrients produced through microbial action. This literature review study aims to analyze the role of probiotics in the fermentation of typical foods in Java, which have the potential to act as an immune regulatory system. The results of a literature study on various kinds of fermented specialties in Java and the manufacturing process show that the microbes that play a role in food fermentation consist of mold and bacteria. These microbes have the potential as an immune regulatory system.  
Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex Sri Wahyuni; R. Susanti; Retno Sri Iswari
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.261 KB) | DOI: 10.22146/ijbiotech.24200

Abstract

This research aims to isolate and characterize the fcin enzyme from Ficus septica stem latex. Ficin from Ficus septica stem latex was isolated using column chromatography. Then enzyme activity was tested at different temperature (40oC, 50oC, 60oC, 70oC) and pH (6.0, 7.0, 8.0) levels. Ficin enzyme activity of joint treatment with variations in temperature and pH was analyzed using two-way ANOVA with a factorial pattern followed by Least Signifcant Difference (LSD) test. The results showed that temperature treatment signifcantly affects enzyme activity. However, the treatment of pH and the interaction between temperature and pH did not signifcantly affect the fcin enzyme activity. There was no signifcant difference in fcin activity at the incubation temperatures of 40oC and 50oC, as well as 60oC and 70oC. However, comparing the incubation temperatures of 40oC and 50oC with treatment 60°C and 70°C showed a signifcant difference in fcin enzyme activity. In the treatment of incubation at pH 6, 7 and 8 for fcin enzyme activity showed no signifcant difference. We concluded that the Ficus septica plant latex contained fcin enzyme with an optimum temperature of 60°C and optimum pH of 6, 7, and 8.
Metagenomic analysis of intestinal microbiota in geese from different farming systems in Gunungpati, Semarang R Susanti; Ari Yuniastuti; Fitri Arum Sasi; Muchamad Dafip
Indonesian Journal of Biotechnology Vol 25, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.53936

Abstract

The diversity of intestinal bacteria in geese correlates with environmental conditions, rearing methods, and consumed feeds. The intestinal bacteria composition is useful for the absorption of nutrition, improving the metabolism, and may be related to the immune system. This study was conducted to examine the intestinal bacteria composition and the diversity of maintained goose in aviaries and barns. This research was an observational exploratory. Five geese were taken purposively from local breeders in Gunungpati District, Semarang City. A total of 5 g of intestinal contents from each sample was used for microbial genome isolation. Then, the genome was amplified to collect 16S rRNA gene region V3-V4. The amplicons were then sequenced using the next generation sequencing (NGS) method (Illumina high-throughput sequencing; paired-end reads) and analyzed using QIIME2 to identify bacterial species. In addition, GC-MS was performed to identify and measure fatty acid contents in the intestinal. The results showed that both rearing and caged goose contained nine phyla of intestinal bacteria. The number of intestinal bacteria of barn geese (SU) reached 32,748 Operational Taxonomy Units (OTU); higher than aviary geese (SK), which was 11,646 OTU. The intestinal bacteria community in barn geese was approved by Phylum TM7 (Saccharibacteria candidate) (53.18%), followed by Firmicutes (32.51%) and Bacteriodetes (5.42%). Whereas on SK Firmicutes was compiled 49.3 4% of total OTU, TM7 (S. candidate) up to 21.17%, and Actinobacteria up to 15.99 %. The abundance of TM7 may contribute to high 9,12-octadecadienoic acid production, while Firmicutes was related to the high production of oleic acid. Based on these data, the reared geese had a more abundant diversity of bacteria than the caged one.
Pengembangan Taman TOGA Untuk Peningkatan Kesehatan Warga Panti Asuhan Yafikhi Semarang R. Susanti; Wulan Christijanti; Noor Aini Habibah; Dewi Mustikaningtyas; Fitri Arum Sasi; Damar Galih; Sasabila Mumtaz; Anisa Dewi Sri Puspitasari
ABDIMASKU : JURNAL PENGABDIAN MASYARAKAT Vol 6, No 1 (2023): Januari 2023
Publisher : LPPM UNIVERSITAS DIAN NUSWANTORO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33633/ja.v6i1.1066

Abstract

Panti Asuhan Yafikhi memiliki lahan kosong yang berpotensi digunakan sebagai perkebunan. Tantangan dalam pengembangan pertanian hortikultura adalah keseimbangan penggunaan bahan organik dan anorganik. Tujuan dari kegiatan pengabdian ini adalah untuk memberdayakan warga panti asuhan Yafikhi dalam (1) merancang arsitektur landscape “Taman TOGA” sesuai dengan luas dan kondisi lahan, (2) mengumpulkan dan menanam toga sesuai dengan landscape yang telah dibuat, (3) Menerapkan bioteknologi dalam pemanfaatan limbah rumah tangga menjadi pupuk organik, (4) merawat dan mengelola kebun TOGA menggunakan pupuk organik, (5) memanfaatkan tanaman obat untuk menjaga kesehatan. Metode yang digunakan adalah workshop, praktik, dan pendampingan. Hasil pengabdian menunjukkan peningkatan pemahaman warga mitra (Panti Asuhan Yafikhi Semarang) tentang pentingnya tanaman obat untuk menjaga kesehatan, terampil merancang dan mewujudkan Taman TOGA, terampil mengelola sampah rumah tangga menjadi kompos padat dan pupuk organik cair, dan terampil menggunakan pupuk organik untuk memelihara taman TOGA.
PENERAPAN TEKNOLOGI AKUAPONIK PADA PEMBERDAYAAN MASYARAKAT KELURAHAN KALISEGORO R. Susanti; Sugianto Sugianto; Tri Sri Noor Asih; Fitri Arum Sasi; Talitha Widiatningrum; Naina Rizki Kenarni; Riska Laila Mukaromah; Farah Fitrotun Nisa’; Adi Franata Jaya
Prosiding Seminar Nasional Biologi Vol. 11 (2023)
Publisher : Prosiding Seminar Nasional Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Isu pangan merupakan persoalan krusial bagi seluruh lapisan masyarakat termasuk masyarakat perkotaan. Pemenuhan kebutuhan pangan dapat dilakukan dengan bertanam atau berternak. Urban farming atau pertanian perkotaan merupakan salah satu kunci pemberdayaan untuk ketahanan pangan berkelanjutan. Budidaya akuaponik merupakan sistem terintegrasi antara akuakultur dan hidroponik, limbah budidaya ikan berupa sisa metabolisme dan sisa pakan dimanfatakan untuk pupuk tanaman. Akuaponik dapat dilakukan dalam skala besar ataupun kecil. Keterbatasan lahan di Kelurahan Kalisegoro Kecamatan Gunungpati, Kota Semarang menyulitkan masyarakat melakukan kegiatan bercocok tanam secara konvensional. Tujuan dari kegiatan pengabdian ini adalah untuk memberdayakan warga Kalisegoro dalam memahami konsep urban farming, khususnya akuaponik, dan terampil menerapkan teknologi akuaponik. Metode yang digunakan adalah (1) participatory urban appraisal, (2) participatory technology development, yaitu workshop pemanfaatan teknologi tepat guna berintegrasi ilmu pengetahuan dan kearifan lokal yaitu akuaponik, (3) pendekatan sosialisasi melalui pendampingan dalam membuat dan merawat akuaponik sampai berhasil. Hasil pengabdian menunjukkan peningkatan pemahaman peserta pengabdian terkait konsep hidroponik dan akuaponik, terampil menyemai bibit tanaman hidroponik, terampil mendesain instalasi akuaponik, terampil menghitung nutrisi yang diperlukan dan terampil merawat akuaponik.
Efek Ekstrak Buah Belimbing Wuluh terhadap Kadar SOD Paru Tikus yang Dipapar Asap Rokok: Effect of Starfruit Extract on SOD Levels in the Lungs of Rats Exposed to Cigarette Smoke Anggun Try Setyaningsih; R. Susanti
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.22267

Abstract

  Cigarette smoke contains a variety of chemical compounds that are harmful to more than 4000 particles that are particularly harmful to cell metabolism such as tar, nicotine, and CO. Cigarette smoke is a free radical that can cause oxidative stress if excessively inhaled in the lungs. Oxidative stress can be characterized by a decrease in SOD levels. SOD enzymes are naturally occurring antioxidants produced in the body to neutralize free radicals. This study aims to find out the effect of Averrhoa bilimbi fruit extract on the lung SOD level of male rats exposed to cigarette smoke. This study used 25 male rats divided into five groups, namely the normal (K) group, the negative group given exposure to cigarette smoke (K-), the treatment group given exposure to cigarette smoke and the extract A. bilimbi fruit 3 mg/kgBB (K1), treatment group given exposure to cigarette smoke and fruit extract A. bilimbi 6 mg/kgBB (K2), treatment group given exposure to cigarette smoke and fruit extract of A. bilimbi 12 mg/kgBB (K3). The antioxidant content of the A. bilimbi fruit extract is analyzed using the DPPH method and the SOD level of the lungs is measured by the calorimetric method. Analysis of SOD data using One Way Anova test and LSD test. The results of the antioxidant test of the A. bilimbi fruit extract showed a 23.37% antioxidant status. The results of statistical tests of A. bilimbi fruit extract on lung SOD levels exposed to cigarette smoke have a real effect. Each group showed real differences in the form of K (79.99%), K- (21.07%), K1 (52.14%), K2 (64.29%), K3 (72.86%). From the results of the research that has been carried out, it can be concluded that the fruit extract of A. bilimbi has an effect on the increase in the SOD level of mice exposed to cigarette smoke.  
Co-Authors Adi Franata Jaya Anggun Try Setyaningsih Anisa Dewi Sri Puspitasari Ari Yuniastuti Asih, Tri Sri Noor Damar Galih Dewi Mustikaningtyas Farah Fitrotun Nisa’ Fidia Fibriana Fitri Arum Sasi, Fitri Arum Margareta Rahayuningsih Muchamad Dafip Naina Rizki Kenarni Noor Aini Habibah Novita Kusuma Wardani Nur Rahayu Utami Retno Sri Iswari Riska Laila Mukaromah Sasabila Mumtaz Sugianto - Syamsul Arifin Talitha Widiatningrum Wulan Christijanti