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INTERAKSI FAMILI × LOKASI PADA UJI KETURUNAN GENERASI KEDUA Eucalyptus pellita Fasis Mangkuwibowo; Sapto Indrioko; Arif Nirsatmanto
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 1 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

The interaction assessment of genotype and environment is necessary to find out an effective selection strategy in progeny trial, particularly in advanced generations. This study aims to observe the effect of family × site interaction in second generation progeny trial of Eucalyptus pellita planted at two locations: Wonogiri, Central Java and Pelaihari, South Kalimantan. The trials design were completely randomized block design (CRBD) with 49 families, 6-10 blocks, 5 tree-plot and 4 m × 1.5 m spacing. Data measurements included height, diameter at breast height, and stem volume at two and four years age. The study results showed that the families had significant differences (p<0.01) for all measured traits at two and four years age, but the family and site interaction was not statistically significant. Family heritability across the two sites were moderate to high for all traits with the type B genetic correlation ranged from 0.67 to 0.94. Heritability and genetic correlation tended to be higher as tree getting older. Less than 16% of families showed an interactive rank changes in the two locations based on multiple-traits selection index. In general, family selection by combining data from the two sites of progeny trial provided higher genetic gain than those by indirect selection, but it was varied to those direct selection on each site. Therefore, these results imply that, the strategy for family selection by combining data from the two sites of progeny trials should be practiced in appropriate combination and direction by considering the magnitude of genetic variation.

KARAKTERISTIK SERAT KAYU HIBRID Acacia auriculiformis x Acacia mangium SEBAGAI BAHAN BAKU PULP Sri Sunarti; Harry Praptoyo; Arif Nirsatmanto
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 2 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Acacia auriculiformis x Acacia mangium hybrid (Aa x Am) is a promising Acacia hybrid for pulp. Wood properties of the hybrid have been reported for their physical and chemical characteristics, but the fiber characteristics have not been investigated. This study was aimed to investigate the fiber characteristics of the hybrid. Wood samples were collected from three years old of Aa x Am hybrid trees planted in a hybrid seed orchard. For comparison, wood sample from other three species: A. mangium x A. auriculiformis hybrid (Am x Aa), A. mangium and A. auriculformis on the same age, planted in the orchard were also collected. The wood sample was prepared from a slice which was put into a bottle containing a combination of acetic acid and hydrogen peroxide (perhidrol) in 1:20 (v/v) for observing fiber characteristics. The results showed that in general the fiber characteristics of Aa x Am hybrid were comparable to those of three comparison species. The average fiber dimensions were intermediate between the two parents: 0.85 mm, 16.09 μm, 12.56 μm and 1.7 μm for fiber length, fiber diameter, fiber lumen width and fiber wall thickness, respectively. The derived values were variable to three comparison species, with the Muhlstep ratio and flexibility coefficient were slightly lower than those observed in Am x Aa hybrid. Based on the level of fiber quality for pulp, the Aa x Am hybrid was classified as level II, the same level as A. mangium and hybrid Am x Aa.

PENGARUH SELEKSI TERHADAP PEROLEHAN GENETIK PADA UJI KETURUNAN GENERASI PERTAMA (F-1) JABON MERAH (Anthocephalus macrophyllus (Roxb.) Havil) DI WONOGIRI Surip Surip; Sapto Indrioko; Arif Nirsatmanto; Teguh Setyaji
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Selection is a fundamental process in the establishment of a progeny trial that will be converted into a seedling seed orchard. In present study, selection practiced in first-generation progeny trial of jabon merah (Anthocephalus macrophyllus Roxb. Havil.) was observed. The study was aimed to find out a criteria selection accompanied with the effective coefficient weight for predicting genetic gain in the first-generation progeny trial of jabon merah. The trial was established in Wonogiri which was laid out in randomized complete block design (RCBD) using 55 families, 4 replications, 4 tree-plot with a spacing of 4 x 2 meter. Measurement was conducted at 40 months after planting covering tree height, diameter at breast height, stem straightness, stem volume. First within-plot selection by culling 2 poorer trees within each plot was practiced at 46 months age. Selection differential from within-plot selection was used to derive coefficient weight which was then applied to predict genetic gain. Results of study showed that selection differential from first within-plot selection were positive for all measured traits. Stem volume was observed as highest priority trait with coefficient weight for each trait were -0.0863 (height), 0.1426 (diameter), 0.2195 (stem straightness), 2.3025 (stem volume). Prediction of genetic gain from within-plot selection were 3,50% (height), 3.58% (diamater), 3.41% (stem straightness), 7.40% (stem volume). While the gain from family selection were 2.80%, 3.75%, 3.54%, 7.10% for height, diamater, stem straightness, stem volume, respectively.

VOLUME TEGAKAN Acacia mangium PADA UJI PEROLEHAN GENETIK DENGAN KERAPATAN TEGAKAN TINGGI Dwi Kartikaningtyas; Teguh Setyaji; Arif Nirsatmanto
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 2 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

High initial stand density is commonly practiced in forest plantations subjected to produce high quality logs through successive thinnings. In this study, genetically improved seed of Acacia mangium was verified in genetic gain trial using high initial stand density. The trial was established in Jonggol, West Java and laid out in randomized complete block design with high initial stand density of 2500 trees/ha or in spacing of 2 x 2 m. The improved seeds tested in the trial were collected from four seedling seed orchards (SSOs). As a control, unimproved seeds from seed production area (SPA) were also planted together in the trial. The assessment of stand volume productivity was periodically conducted at two, three and four years of age. The results of study showed that the improved seeds from the four SSOs out performed the unimproved one. However, the significant difference among the seed sources was initially found at the four years of age. The average stand volumes of improved seed were 58.56m3/ha, 94.87m3/ha and 163.8m3/ha for two, three, and four years of age, respectively. The superiority of improved seed over the unimproved one ranged from 23%-36% (two years), 6%-30% (three years) and 2%-22% (four years). The four SSOs showed different behaviours in response to high stand density. Improved seed from SSOs with higher selection intensity was likely to be more tolerant to high stand density.

EVALUASI PRODUKSI BENIH PADA KEBUN BENIH HIBRID ACACIA (Acacia mangium x Acacia auriculiformis) DI WONOGIRI, JAWA TENGAH Sri Sunarti; Valerianus Devi Adyantara; Suharyanto Suharyanto; Teguh Setyaji; Arif Nirsatmanto
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Research for developing Acacia hybrid (Acacia mangium x Acacia auriculiformis) breeding strategy through establishing hybrid seed orchard (HSO) has been started by BBPPBPTH. Two hectares of HSO was established in Wonogiri, Central Java to produce the Acacia hybrid seed. The orchard was laid out using an alternating rows design among the two pure parent species with spacing of 1 m x 3 m: 1 m within the species and 3 m between the species. The purpose of this study is to evaluate flowering synchronization and seed production in first year flowering session of four years age mother trees in the HSO. The unit area of observation was set up in 0.2 ha within the HSO. During the first flowering session, 100 mother trees (31%) were observed showing synchronized flowering time among the two parents species: 50 mother trees for Acacia mangium and 50 mother trees for A. auriculiformis. Seed production was still low in which only 38 out of 50 trees of A. mangium mother trees produced seed, whereas selected A. auriculiformis mother trees did not produce seed. The average viability of the collected seed was moderately low at 59.2%. Subsequent verification through DNA marker using SCAR and SSR revealed less than 1% of the collected seed were identified as true Acacia hybrid.

SELEKSI DAN PEROLEHAN GENETIK PADA KEBUN BENIH SEMAI GENERASI KETIGA Acacia mangium Betty Rahma Handayani; Sri Sunarti; Arif Nirsatmanto
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

One of the purposes of seedling seed orchard (SSO) establishment is to produce genetically improved seeds. The amount of genetic gain in the SSO is determined from a series of selection involving within and between family selection in which the gain is generally achieved from the accumulated increases of gain in successive advanced generation breeding cycles. In present study, the amount of genetic gain was predicted in third-generation SSO of Acacia mangium after first within-plot selection using a retrospective selection index’s method. The SSO was established in Wonogiri, Central Java which was laid out in a randomized complete block design, 65 families, six replications, four trees-line plot and spacing of 2 m x 4 m. Actual within-plot selection was subjectively practiced in 40 months after planting. The observed traits for genetic gain prediction was height, diameter at breast height (dbh), and stem straightness using data collected at 24 months after planting. For calculating genetic gain based on multiple trait selection index, coefficient weight for each trait was then derived from the selection differential. The results of study showed that the first within plot selection was practiced mostly for imposing dbh rather than height or stem straightness with the relative gain was 6% for dbh, and 3% for both height and stem straightness. Using the derived coefficient weight, the expected genetic gain by family selection was found to be slightly lower than those predicted by within-plot selection at 3% for dbh, and 2% for both height and stem straightness.

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