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PEROLEHAN GENETIK PADA UJI KLON JATI (Tectona Grandis L.F) UMUR 3 TAHUN DI KHDTK KEMAMPO, SUMATERA SELATAN Sofyan, Agus; Indrioko, Sapto
Jurnal Penelitian Hutan Tanaman Vol 8, No 3 (2011): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Peningkatan Produktivitas Hutan

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Abstract

Paternity Analysis of Tea (Camellia sinensis L. Kuntz) Hybrids Using Isozyme Marker Setyorini, Titin; ., Taryono; ., Suyadi; Indrioko, Sapto
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Tea plant has been categorized as self-incompatible crop. This is the reason behind the high genetic diversity. Natural pollination is possible to occur and the male parent is usually unknown, therefore, there is a need of method to identify male parent of hybrids through paternity analysis. Isozyme markers have been successfully used for paternity analysis due to their co-dominant polymorphism. This research aimed to predict male parents of hybrids by figuring out the mating system through isozyme banding patterns. In this experiment, seven enzyme systems were evaluated, of which only two of the enzyme systems i.e. esterase and shikimate dehydrogenase showing clear band pattern of Est-1, Est-2, and Shd-1 loci. The mating system of tea could be categorized as a mixed mating model, with high estimated out-crossing rate of 98.6 %. The pollen contributors were not always originated from the vicinity of the female parents.Key words: isozyme markers, paternity analysis, tea
Allozyme variation of the endemic and vulnerable Dyera lowii Hook.f. in Central Kalimantan: Implications for genetic resources conservation Wahyudiningsih, Tri Suwarni; Naiem, Mohammad; Indrioko, Sapto; Sumardi, Issirep
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

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Abstract

Dyera lowii is an endemic and vulnerable tree species of commercial value as chewing gum found inpeat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existenceis now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity withinfour natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in CentralKalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectricfocusing polyacrylamide slab gel system. The result showed high genetic diversity (HE=0.52) and gene fl ow(3.402) seemed to be effective. A total of 14 alleles were found among all the analysed population. Meannumber of alleles per locus (Aa) was 3.206, and the effective number of alleles per locus (Ae) was 2.21. Geneticdifferentiation between populations (FST) was signifi cant at the moderately level (0.0685). Most allozymevariation was found within population (93.2%). Special attention is essential to conserve a private allele ofGot-1-e (9%) at Selat Nusa population. Sebangau population missed the alleles of Est-2-b and Got-1-a, as foundin other populations. Selat Nusa population is expected to enhance the effective management for geneticresources conservation of this vulnerable species in the future.
EVALUASI DAMPAK PROGRAM BENIH UNTUK RAKYAT (SEED FOR PEOPLE) DI DESA KASEGERAN, KECAMATAN CILONGOK, KABUPATEN BANYUMAS Farobi, Ibnu; Na'iem, Mohammad; Sabarnurrdin, Sambas; Indrioko, Sapto
Jurnal Wana Tropika Vol 5 No 2 (2015)
Publisher : Fakultas Kehutanan Institut Pertanian STIPER Yogyakarta

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Abstract

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INTERAKSI FAMILI × LOKASI PADA UJI KETURUNAN GENERASI KEDUA Eucalyptus pellita Fasis Mangkuwibowo; Sapto Indrioko; Arif Nirsatmanto
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 1 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (569.377 KB) | DOI: 10.20886/jpth.2018.12.1.25-39

Abstract

The interaction assessment of genotype and environment is necessary to find out an effective selection strategy in progeny trial, particularly in advanced generations. This study aims to observe the effect of family × site interaction in second generation progeny trial of Eucalyptus pellita planted at two locations: Wonogiri, Central Java and Pelaihari, South Kalimantan. The trials design were completely randomized block design (CRBD) with 49 families, 6-10 blocks, 5 tree-plot and 4 m × 1.5 m spacing. Data measurements included height, diameter at breast height, and stem volume at two and four years age. The study results showed that the families had significant differences (p<0.01) for all measured traits at two and four years age, but the family and site interaction was not statistically significant. Family heritability across the two sites were moderate to high for all traits with the type B genetic correlation ranged from 0.67 to 0.94. Heritability and genetic correlation tended to be higher as tree getting older. Less than 16% of families showed an interactive rank changes in the two locations based on multiple-traits selection index. In general, family selection by combining data from the two sites of progeny trial provided higher genetic gain than those by indirect selection, but it was varied to those direct selection on each site. Therefore, these results imply that, the strategy for family selection by combining data from the two sites of progeny trials should be practiced in appropriate combination and direction by considering the magnitude of genetic variation.
PARAMETER GENETIK SIFAT PERTUMBUHAN DAN KERAPATAN KAYU KLON Eucalyptus pellita F. Muell. DI DUA TAPAK YANG BERBEDA DI KALIMANTAN TIMUR Achmad Ramadan; Sapto Indrioko; Eko Bhakti Hardiyanto
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (848.087 KB) | DOI: 10.20886/jpth.2018.12.2.115-125

Abstract

Industrial forest plantations have an important role in fulfilling the wood demand. Based on global industrial development, the forest plantations industry will increase in the following years. Eucalyptus pellita has become main species in Indonesia forest plantations because it has a short cycle and wood products are suitable to forest industry. The average productivity of E. pellita plantations in Indonesia is still low and high variation. In an effort to increase the productivity, the first step is a better understanding of genetic control on growth and basic density. This study aims to determine the genetic parameters for growth and basic density of E. pellita clones on two different sites. The clonal trials was conducted at two different sites. Both clonal trial are designed using RCBD. The number of clones tested in both trial was 30, 5 blocks and 25 tree/plot. The result of the study showed that the effects of clones vary greatly according the enviromental conditions. The clones- environemntal interaction of growth trait is higher than the basic density. This is in line with genetic parameters of growth trait that are less stable than the basic density. The expected genetic gain of growth trait is higher than the basic density and at the same time there was a weak genetic correlation (there is even a negative) between growth trait and basic density. Therefore carefulness is needed in selecting clones when the two traits are used as selection parameters.
KERAGAMAN GENETIK CENDANA PADA TEGAKAN PENGHASIL BENIH DAN TEGAKAN REHABILITASI DI NUSA TENGGARA TIMUR BERDASARKAN PENANDA ISOZIM Rini Purwiastuti; Sapto Indrioko; Eny Faridah
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2016.10.1.23-30

Abstract

This study aimed to determine the genetic diversity of sandalwood on seed production stand and rehabilitation stand in East Nusa Tenggara using isozyme genetic marker. The study was conducted using samples collected from three stands, i.e. Seed Production Area (APB) representing seed production stand, KHDTK rehabilitation stand and CSR rehabilitation stand representing rehabilitation stands. Samples of sandalwood juvenile leaves are taken randomly from each location. Sample materials taken were juvenile leaves collected randomly from each stand. There were 57 samples taken from APB, while each 25 samples were collected from rehabilitation stands of KHDTK and CSR. Isozymes analyses were carried out in the laboratory using three kinds of enzyme systems i.e Esterase (EST), Diaphorase (DIA) and Shikimate Dehydrogenase (SHD). The results showed that for genetic diversity within stands, the mean of polymorphic loci was 88.89%, with a mean number of alleles per locus 2.1667 and a mean of effective alleles 1.2103. The expected heterozygosity within stands (HS) was 0.1558, with the observed heterozygosity (HO) of 0.1402, while the mean index of fixation (FIS) was 0.1118. On genetic diversity among stands, DST, and GST values were 0.0090 and 0.0545 respectively, while total expected heterozygosity of the three stands (HT) was 0.1648. To anticipate sandalwood genetic diversity decline, it is essential to identify and record the remaining sandalwood populations, then conserve rare alleles either through in-situ or ex-situ conservation programs.
STUDI KERAGAMAN GENETIK Diospyros rumphii Bakh DI SULAWESI UTARA BERDASARKAN PENANDA ISOENZIM Julianus Kinho; Mohammad Na&#039;iem; Sapto Indrioko
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 2 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.148 KB) | DOI: 10.20886/jpth.2016.10.2.95-109

Abstract

Diospyros rumphii Bakh. is one of tropical commerciall wood species grouped in ebony. Ebony is the name of some species of commercial timber from genus of Diospyros (Ebenaceae). Seven species of them are commercial wood, which in the trade is known as ebony namely; D.celebica, D.rumphii, D.pilosanthera, D.lolin, D.ebenum, D.ferrea and D.macrophylla. Two of seven species ebony have the highest economic values namely; D.celebica and D.rumphii. Nowadays, the population D. rumphii in the nature are decreases due to over harvesting in the past. This study aims to determine genetic diversity of D. rumphii within populations, between populations and its relationship. The samples of D. rumphii in this study from four populations in North Sulawesi i.e Bitung, Talise, Talaud and Bolaang Mongondow. Genetic diversity was detected by isoenzym markers with seven enzyme i.e Glutamate oxaloacetate transmirase (GOT), Shikimate dehydrogenase (SHD), Esterase (EST), Acid phoshatase (ACP), Diaphorase (DIA), 6-Phosphogluconate dehydrogenase (6PG) and Glutamate dehydrogenase (GDH). The results showed that there are three enzym was detected polymorphisme i.e EST, GOT and GDH. Genetic diversity generated by three enzyme systems who are controlled by 5 loci and 15 alleles. The average percentage of polymorphic loci was100%. The number of total genetic diversity is 0.64,which are distributed within population 0.59 (91.24%) and between population 0.05(8.76%). Cluster analysis using Unweighted Pair Group Method with Aritmatic Means (UPGMA) based on Nei standard genetic distance showed three clusters:Bitung and Bolaang Mongondow, Talise, and Talaud.
INDUKSI TUNAS, MULTIPLIKASI DAN PERAKARAN Gyrinops versteegii (Gilg.) Domke SECARA IN VITRO Aziz Akbar M; Eny Faridah; Sapto Indrioko; Toni Herawan
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (584.452 KB) | DOI: 10.20886/jpth.2017.11.1.1-13

Abstract

Gyrinops versteegii (Gilg.) Domke is including one of superior agarwood-producing plants and naturally growing in Eastern Indonesia as Nusa Tenggara and Papua. Indonesia has been trading agarwood products both domestically and overseas which one of them is agarwood produced by G.versteegii. This study purpose was to develop an in vitro culture method for mass propagation of G. versteegii. Shoot induction conducted on MS medium supplemented with Benzyl Amino Purine (BAP) 0.7; 1.0; 1.5; and 2.0 mg/l. The shoots multiplication conducted on MS medium supplemented with the best concentration of BAP from shoot induction phase. The rooting of shoots conducted on half strength MS medium supplemented with interaction of Napthalene Acetic Acid (NAA) 0.01 mg/l with concentration of Indole-3-Butyric Acid (IBA) 1.0; 2.0; 3.0; and 4.0 mg/l. Epicotyl explant with a given concentration level of BAP 0.7 mg/l produce the highest rates number of shoots and shoot length compared to other explant respectively 4.8 shoots and 0.41 cm within 6 weeks. The best explant developments in the best medium able to promote the growth of the length and number of shoots are 0.28 shoots and 0.3 cm within 4 weeks. Explants easiest, quickest and most high sprouting ability as a factor of success in terms of multiplication is epicotyl. The combination treatment of material explant with concentration of BAP only affect to growth of shoots length. The combination treatment of NAA with concentration of IBA has no effect against root formation and growth rootlength.
RESPON PERTUMBUHAN TANAMAN JATI PLUS PERHUTANI UMUR 11 TAHUN TERHADAP INTENSITAS PENJARANGAN DAN TUMPANG SARI (STUDI KASUS DI BKPH BEGAL KPH NGAWI, PERHUTANI JAWA TIMUR) Muhammad Fadhil Amiruddin Sudomo; Suryo Hardiwinoto; Sapto Indrioko; Budiadi Budiadi; Daryono Prehaten; Aris Wibowo
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 1 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.1.13-23

Abstract

Thinning is one of silvicultural management actions in maintaining and increasing growth and quality of standing stands. This study aims to determine growth response of selected teak clone to several intensities of thinning and intercropping. The method was a factorial completely randomized block design (RCBD) with thinning intensity (4 levels): 0% (control = A1), 25% (A2), 50% (A3) and 75% (A4) as the main plot, while intercropping (2 levels), as the sub plot. The results showed that the Current Annual Increment (CAI) of diameter at breast height (CAI DBH), volume/tree (CAI vol/tree) and volume/hectare (CAI vol/ha) of stands gave a significant positive response to thinning intensity treatment. CAI DBH one year after thinning were 0.79 cm, 1.47 cm, and 2.46 cm; increased by 32%, 145% and 310% at the thinning intensity of 25%, 50% and 75% when they compared with the control (0.59 cm). CAI vol/ha of stand in the first year, namely 11.88 m/ha, 14.04m3/ha, and 16.78 m3/ha or with an increase of 9%, 29% and 54% respectively at an intensity of 25 %, 50% and 75% compared to the control.