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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
ILG Nurtjahjaningsih
Center for Forest Biotechnology and Tree Improvement Research, Development and Innovation Ministry of Environment and Forestry
Agriculture, Biological Sciences & Forestry

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STRUKTUR GENETIK POHON INDUK Calophyllum inophyllum DI TEGAKAN BENIH PROVENAN BERDASARKAN PENANDA SIMPLE SEQUENCES REPEATS ILG Nurtjahjaningsih; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 13, No 1 (2019): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.676 KB) | DOI: 10.20886/jpth.2019.13.1.45-51

Abstract

This study aimed to assess genetic structure of Calophyllum inophyllum using simple sequences repeats (SSR) markers to control genetic diversity at a provenance seed stand. Leaf samples were collected for DNA templates and 6 SSR primers were used. Genetic data of 280 trees were analyzed by FSTAT software, while PCoA was analyzed by GeneAlex. Results showed that number of allele (NA) per SSR primer between 3 and 4, the allelic richness (AR) ranged 2.805 to 4.000, the gene diversity (HE) ranged 0.018 to 0.631, coefficient inbreeding (FIS) of overall SSR primers were insignificant deviate from Hardy-Weinberg Equilibrium, excepted NY3. The mean HE values in the TBP was in low (mean HE= 0.272). However, the mean coefficient inbreeding values was not significant. PCoA analysis showed that the seed stand consisted genetically related trees; the 280 trees originated from only 5-6 mother trees. This is because the stand was originated from a conservation stand with narrow genetic differentiation. Management of the seed stand was discussed.
PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis) ILG Nurtjahjaningsih; Toni Herawan; Reza Permatasari Rachma; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2018.12.2.127-134

Abstract

This study aimed to test RAPD markers to assess genetic stability of teak clones. Two experimental steps were carried out. First, nine RAPD markers were screened to verify the level of polymorphic loci; second, the polymorphic loci were applied to test genetic stability of clones. To test polymorphism levels of the primers, DNA was isolated from eight leaf samples that were collected from a seed orchard located at Watusipat, Gunung Kidul. To verify genetic stability of clones, DNA was isolated from leaf samples of 24 ramets of 3 clones after second sub-culturing. Results showed that amplification of 5 out of 9 RAPD primers were be consisten and produced 12 polymorphic loci. The number of polymorphic alleles per locus ranged between 1 and 3; the allele sizes were between 400 and 1,050 base pairs (bps). The percentage of polymorphic loci was 100%; it meant that overall loci have high polymorphism level. Based on these loci showed that the 24 ramets are clones; there was no somaclonal variation or high genetic stability. However, these loci need to be validated using more stable DNA markers.
KERAGAMAN GENETIK POPULASI KAYU KUKU (Pericopsis mooniana, (THWAITES)THWAITES) DI HUTAN LAMEDAI BERDASARKAN PENANDA RAPD ILG Nurtjahjaningsih; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 13, No 1 (2019): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.414 KB) | DOI: 10.20886/jpth.2019.13.1.25-32

Abstract

Vulnerable status of kayu kuku (Pericopsis mooniana) is due to heavily wood harvesting. Aim in this study is to asses genetic diversity and structure of kayu kuku at Lamedai forest, Southeast Sulawesi using RAPD markers. Leaves samples were collected from 4 natural populations at Lamedai forest i.e. Lamedai natural reserve, Lamedai village, Balijaya and Tangketada. The 22 polymorphic loci of 5 RAPD markers were subjected to the genetic analysis, binary data were calculated by GenAlex software to obtain parameters in genetic diversity within population and analysis molecular variant (AMOVA), while PopTree software was used to construct a dendrogram. Results showed that genetic diversity of the 4 populations was in moderate level (mean He: 0.361 ± 0.017) and the values was similar each other. There was no private allele at any populations, indicating that there was no genetic specification. A dendrogram analysis showed that Lamedai natural reserve genetically closed to Tangketada population and Balijaya, while Lamedai was separated into another cluster. AMOVA analysis showed 13% genetic diversity were observed among populations. In conclution, kayu kuku at Lamedai forest might be a large and continuous population in ancient period.
Co-Authors Anto Rimbawanto Anto Rimbawanto AYPBC Widyatmoko Purnamila Sulistyawati Reza Permatasari Rachma Toni Herawan