Toni Herawan
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta

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KULTUR JARINGAN CENDANA (Santalum album L.) MENGGUNAKAN EKSPLAN MATA TUNAS Toni Herawan; Mohammad Na'iem; Sapto Indrioko; Ari Indrianto
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 3 (2015): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.632 KB) | DOI: 10.20886/jpth.2015.9.3.177-188

Abstract

The research aim is to observe tissue culture method for Sandalwood using node explants. The explants were cultured on Murashige and Skoog (MS) medium solidified with agar and supplemented with varies combination of hormones: BAP (benzyl-amino-purine), NAA (napthalene-acetic-acid), IAA (indole-acetic-acid) and Kinetin (furfuril-amino-purine) for shoot induction, multiplication and rooting. The results of study showed that the medium of MS+1 mg/l BAP+0.01 mg/ lNAA provided a good response for shoot induction of Sandalwood clones number A.III.4.14 at around 85%. The medium of MS+0.5 mg/l BAP+0.01 mg/l NAA provided a good response for shoot multiplication of the clones number A.III.4.14 (number of shoot and shoot elongation). The rooting medium of ½MS+20 mg/l IAA+1 mg/l IAA and 0.01 mg/l NAA resulted rooting percentage across the clones at around 37%. The highest survival rate after acclimatization was found at clone number WS6 at around 56%.
Pengaruh Gibberellin (GA4) terhadap Waktu Perkecambahan dan Pertumbuhan Tinggi Semai Cendana (Santalum Album Linn.) Asri Insiana Putri; Toni Herawan
Jurnal Ilmu Kehutanan Vol 2, No 2 (2008)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3989.827 KB) | DOI: 10.22146/jik.821

Abstract

Effect of Gibberellins (GA4) on Germination Time and Height of Cendana (Santalum album Linn.) The hemiparasite Santalum album Linn. (cendana) grows very slow, in nature the rare and difficult seeds need stimulation to germinate. Gibberellins (including GA4) are growth regulators, usually used to increase growth as well as to break seed dormancy. The objectives of this research were to investigate the influence of gibberellins on germination percentage and height of cendana growth. Experiment was laid out in a Completely Randomized Design with 3 replicates of 300 seeds for germination percentage and 3 replicates of 10 seeds for seedling growth. Gibberellin was applied as treatment with 100, 300, and 500 ppm. The seed germination was recorded until 9 weeks, and height of plants measured until 8 months at the greenhouse. The results showed that the addition of gibberellins at all treatment increased the percentage of germination and caused the seeds germinated four weeks earlier than the control. In the first 4 months, 500 ppm gibberellins gave the highest acceleration of germination, afterward all treatments have relatively the same influences. Gibberellins gave positive effect on height of cendana growth. After 7 months, the growth decreased although all gibberellin treatments gave higher growth than the control.