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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
Mohammad Na'iem
Fakultas Kehutanan Universitas Gadjah Mada Yogyakarta
Agriculture, Biological Sciences & Forestry

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KULTUR JARINGAN CENDANA (Santalum album L.) MENGGUNAKAN EKSPLAN MATA TUNAS Toni Herawan; Mohammad Na'iem; Sapto Indrioko; Ari Indrianto
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 3 (2015): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.632 KB) | DOI: 10.20886/jpth.2015.9.3.177-188

Abstract

The research aim is to observe tissue culture method for Sandalwood using node explants. The explants were cultured on Murashige and Skoog (MS) medium solidified with agar and supplemented with varies combination of hormones: BAP (benzyl-amino-purine), NAA (napthalene-acetic-acid), IAA (indole-acetic-acid) and Kinetin (furfuril-amino-purine) for shoot induction, multiplication and rooting. The results of study showed that the medium of MS+1 mg/l BAP+0.01 mg/ lNAA provided a good response for shoot induction of Sandalwood clones number A.III.4.14 at around 85%. The medium of MS+0.5 mg/l BAP+0.01 mg/l NAA provided a good response for shoot multiplication of the clones number A.III.4.14 (number of shoot and shoot elongation). The rooting medium of ½MS+20 mg/l IAA+1 mg/l IAA and 0.01 mg/l NAA resulted rooting percentage across the clones at around 37%. The highest survival rate after acclimatization was found at clone number WS6 at around 56%.
ANALISIS SISTEM PERKAWINAN MERBAU (Intsia bijuga O. Ktze) DI PAPUA BERDASARKAN PENANDA ISOENZIM Mahfudz Mahfudz; Mohammad Na'iem; Sumardi Sumardi; E B Hardiyanto
Jurnal Pemuliaan Tanaman Hutan Vol 4, No 2 (2010): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2010.4.2.109-116

Abstract

Merbau (Intsia bijuga O. Ktze) is an important species to supply wood in Indonesia. Mating system on natural population of merbau can influence genetic diversity of merbau. The objectives of this study were to investigate mating system on natural forest of merbau in Papua. The research used 200 samples of merbau from 10 mother trees in Gunung Meja Forest Research Manokwari-Papua. Isoenzyme analysis was done using four enzyme systems i. e. Peroxidase (POD), Esterase (EST), Glutamate Oxaloacetate Transaminase (GOT) dan Diaphorase (DIA). Data were analysed using MLTR program. The result showed that average genetic diversity (He) 0.463 and total heterozygosity (HT) 0.496. The multilocus population outcrossing rate (tm) and singlelocus population outcrossing rate (ts) were tm = 1.00 and ts = 0.992. Probability of inbreeding rate (tm  - ts) = 0.008.
SENYAWA FENOL PADA TOLERANSI Falcataria moluccana (Miq.) TERHADAP PENYAKIT KARAT TUMOR Asri Insiana Putri; Mohammad Na'iem; Sapto Indrioko; Sri Rahayu
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 3 (2015): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.302 KB) | DOI: 10.20886/jpth.2015.9.3.189-202

Abstract

Falcataria moluccana (Miq.) is severely attacked by Uromycladium tepperianum (Sacc.) McAlpine, which is a gall-forming (neoplasmic) and parasitic obligate pathogen. Phenolic compounds have the ability to function as co-factors of pathogenicity determinant of pathogens development and chemical defenses of plants. The purpose of this study was to determine the content of phenolic compounds on gall rust tolerance sengon by observation of tolerant sengon height, quantitative analysis of total phenolic compounds, wood anatomy analysis and tolerance test of sengon callus from tissue culture with filtrate gall rust as chemical agent. The results of the research were (1) inoculated sengon have lower total phenolic compounds than the control (no inoculation), (2) tolerant sengon have lower content of phenolic compounds than sensitive one, (3) microscopic wood anatomy observation shows that tolerant sengon have darker substrat, and (4) in the same concentration of gall rust filtrate incubation media, the tolerant sengon have higher survival cell calli. The highest concentration that callus cells can survive was 25% (v/v).
Co-Authors Ari Indrianto Asri Insiana Putri E B Hardiyanto Mahfudz Mahfudz Sapto Indrioko Sri Rahayu Sumardi Sumardi Toni Herawan