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Arief Husin
Universitas Muhammadiyah Purwokerto
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health

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Journal : Sainteks

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Turmeric Flour Supplementation Through Feed-in Biofloc System Cultivation on the Growth of Nilem Fish (Osteochilus hasselti) Cahyono Purbomartono; Dhika Rahma Fauziyyah; Arief Husin
Sainteks Vol 19, No 1 (2022): April
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v19i1.13410

Abstract

Nilem fish (Osteochilus hasselti) is favored by the community because of its chewy meat texture, good taste, and relatively cheap price, so it has the potential to be developed. However, the growth of nilem fish is slow therefore it needs to be given an immunostimulant to increase its growth. Biofloc is known as a cultivation system that can maintain water quality and provide sufficient floc for additional fish feed. This study aims to determine the effect of using turmeric flour in feed to increase the growth of nilem fish reared in the biofloc system. The study used a completely randomized design (CRD), with 3 treatments and 1 control each repeated 4 times. The treatments were supplementation of turmeric flour through feed at a dose of 1.125 g (B), 0.75 g (C), and 0.375 g (D) kg-1 feed and control (A) in cultivation with a biofloc system. The study lasted for 90 days with feeding 3%/kg of biomass weight given 2 times a day, in the morning and evening. Parameters observed in this study were weight (WG) and length growth (LG), daily growth rate (SGR), feed conversion ratio (FCR) and feed efficiency (EF). The data collection method was carried out by weighing and measuring the weight and length of the fish and the amount of feed consumed. The data obtained were collected and analyzed using SPSS 2.0.The results showed that supplementation of turmeric flour through feed reared in the biofloc system could increase weight and length growth, daily growth rate, feed conversion ratio, and feed efficiency. The optimal dose was obtained in the treatment of 1.125 g kg-1 feed (B), proving that supplementation of turmeric flour through feed has the potential to be applied to fish culture using the biofloc system.
Non-Specific Immune Potentiating activity of Multivitamins in Catfish (Clarias sp.) Cahyono Purbomartono; Ulfatun Rizkiyah; Dini Siswani Mulia; Arief Husin
Sainteks Vol 18, No 2 (2021): Oktober
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v18i2.13113

Abstract

Fish farming is well developed because many people like to consume fish. The main problem in cultivation is an attack of a disease. Generally, the disease in fish is MAS (Motile Aeromonas Septicemia) which is caused by Aeromonas hydrophila. Prevention of diseases in fish can be done by increasing immunity through the addition of multivitamins in feed. This study aims to determine the ability of multivitamins against non-specific immune responses in African catfish (C. gariepinus) as well as to know the optimum dose. The study used an experimental method with a completely randomized design using 3 treatments, 1 control, and 4 individual replications. The treatment using a multivitamin through feed with a dose of T1 = 2 g kg-1, T2 = 2.5 g kg-1, T3 = 3 g kg-1 and T0 = control. The parameters observed were phagocytic activity, and differential leukocytes of monocytes and lymphocytes. Data were analyzed using Analysis of variance (ANOVA) at the 95% confidence level. If there is a real difference, then proceed with the Duncan Multiple Range test Test (DMRT) at a 95% confidence level. The results showed that the addition of multivitamins through feed on African catfish had a significant effect (p<0.05) on phagocytic activity and differential leukocyte of monocytes, but had no significant effect (p> 0.05) on differential leucocytes of lymphocyte. The optimum dose for increasing non-specific immune responses in African catfish is T2 at a dose of 2.5 g kg-1 of feed. Based on research, the addition of multivitamins through the feed can potentially be used in fish farming to increase immunity.
Kandungan Asam Amino Tepung Bulu Ayam yang Difermentasi dengan Bacillus licheniformis B2560 dan Bacillus subtilis Sebagai Bahan Baku Pakan Ikan Dini Siswani Mulia; Arief Husin; Juli Rochmijati Wuliandari
Sainteks Vol 18, No 2 (2021): Oktober
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v18i2.13067

Abstract

Kualitas pakan ikan salah satunya ditentukan oleh kandungan protein dan asam amino. Penelitian bertujuan untuk mengkaji kandungan asam amino tepung bulu ayam yang difermentasi dengan Bacillus licheniformis B2560 dan Bacillus subtilis sebagai bahan baku pakan ikan. Penelitian menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL) pola faktorial, yaitu perlakuan I : fermentasi (A1 : kontrol/non fermentasi, A2 : fermentasi dengan B. licheniformis B2560, A3 : fermentasi dengan B. subtilis), dan perlakuan II : jumlah inokulum (B1 : jumlah inokulum 5 mL, B2 : jumlah inokulum 10 mL, B3 : jumlah inokulum 15 mL, masing-masing untuk tepung bulu ayam sebanyak 2 g). Parameter penelitian yang diamati adalah kandungan asam amino. Data dianalisis menggunakan Analysis of Variance (Anova) dan dilanjutkan dengan Duncan Multiple Range Test (DMRT) pada taraf uji 5%.  Hasil penelitian menunjukkan bahwa fermentasi tepung bulu ayam menggunakan B. licheniformis B2560 dapat meningkatkan kandungan beberapa jenis asam amino lebih tinggi dibandingkan dengan B. subtilis. Jumlah inokulum 15 mL lebih optimal dalam mendegradasi protein keratin menjadi asam amino. Jenis asam amino dengan kandungan yang sesuai untuk kebutuhan ikan adalah asam glutamat, serin, glisin, treonin, arginin, tirosin, valin, fenilalanin, isoleusin, dan leusin.
Effectiveness of Herbal Diet Nigella sativa and Gracilaria verrucosa Against Non-specific Immunity of Tilapia (Oreochromis niloticus) Cahyono Purbomartono; Rofiqoh Rofiqoh; Arief Husin; Dini Siswani Mulia
Sainteks Vol 20, No 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v20i1.16410

Abstract

The addition of Nigella sativa and Gracilaria verrucosa to feed is used as a supplement to increase non-specific immunity in tilapia. The addition of herbal supplements is safe for consumption, easy to decompose, has no residue on the fish's body, and does not harm the environment. This study aims to find the effectiveness of the addition of the herbs Nigella sativa and Gracilaria verrucosa to increase non-specific immunity in tilapia. The study used an experimental method with a completely randomized design, applying 4 treatments and 1 control to tilapia. The dietary doses of Nigella sativa were T0 (control, without Nigella sativa), T1 (2.5), T2 (5), T3 (7.5), and T4 (10) (g kg-1 feed). While the dietary doses of Gracilaria verrucosa were T0 (control, without G. verrucosa), T1 (0.5), T2 (1), T3 (1.5), and T4 (2) (g kg-1 feed). The results showed that the percentage of hematocrite increased significantly with the Nigella sativa diet, while on the Gracilaria verrucosa diet, the percentage of hematocrit was not significant. Further, the Nigella sativa diet and the Gracilaria verrucosa diet can significantly increase the percentage of lymphocytes, but not significantly to the percentage of monocytes. In fish farming, the Nigella sativa and Gracilaria verrucosa diets have the potential to be used to increase non-specific immunity in tilapia, but it is more effective to use the Nigella sativa diet at a dose of 5 g kg-1 feed.
Efektivitas dan Potensi Herbal untuk Peningkatan Pertumbuhan Benih Lele dumbo (Clarias gariepinus) Cahyono Purbomartono; Arief Husin; Irene Septya Bagasnabila; Fani Giri Dwi Zularini; Alvia Trisakti Susiyani; Eka Puji Purwaningsih; Purnomo Purnomo
Sainteks Vol 19, No 2 (2022): Oktober
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v19i2.14903

Abstract

Potensi perikanan Indonesia sangat besar namun belum berhasil seluruhnya dimanfaatkan secara optimal. Banyak faktor yang mempengaruhi tingkat keberhasilan seperti adanya hambatan penyakit yang dapat mempengaruhi pertumbuhan. Pertumbuhan yang optimal merupakan factor utama agar dapat mencapai produktivitas biomass ikan yang optimal. Penelitian ini bertujuan untuk menemumukan efektivitas herbal akuatik maupun teresterial untuk memperoleh pertumbuhan optimal. Metode penelitian adalah eksperimen laboratorium menggunakan Rancangan Acak Lengkap (RAL), masing-maisng dengan 3 ulangan. Hasil penelitian membuktikan herbal teresterial temulawak yang dikombinasikan dengan bawang putih dapat meningkatkan semua indikator pertumbuhan yang signifikan dibanding kontrol dengan dosis optimal 20 g temulawak + 4,50 g bawang putih pada lele dumbo. Demikian pula diet herbal teresterial Aloe vera lebih efektif dibanding diet bawang putih yang ditunjukkan dengan capaian berat akhir dan pertambahan berat yang signifikan pada lele dumbo. Selanjutnya, kombinasi herbal akuatik dan teresterial yaitu diet (fucoidan + kunyit) lebih efektif dibanding hanya pemberian diet tunggal fucoidan saja pada lele dumbo dengan meningkatnya pertambahan berat dan panjang yang lebih optimal. Herbal akuatik fucoidan maupun herbal teresterial temulawak, bawang putih, Aloe vera dan kunyit berpotensi digunakan untuk meningkatkan pertumbuhan pada budidaya ikan yang dapat diberikan secara tunggal maupun kombinasi.
Co-Authors Alvia Trisakti Susiyani Dhika Rahma Fauziyyah Dini Siswani Mulia Eka Puji Purwaningsih Fani Giri Dwi Zularini Heri Maryanto, Cahyono Purbomartono, Heri Maryanto, Irene Septya Bagasnabila Juli R. Wuliandari, Juli R. Kiki Ernawati Listika Yusi Risnani Purnomo Purnomo Rofiqoh Rofiqoh Sisunandar Sisunandar Ulfatun Rizkiyah