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Clonal Propagation of Vetiveria zizanioides L. through Tissue Culture Technique Wahyu Widoretno; Arbaul Fauziah; Serafinah Indriyani; Edi Priyo Utomo
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 2, No 1 (2017)
Publisher : Institut Atsiri Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.814 KB) | DOI: 10.21776/ub.ijeo.2017.002.01.05

Abstract

 In vitro propagation method for vetiver (Vetiveria zizanioides L.) had been effectively developed in this study. Several variations of media were used for shoot initiation and multiplication. Shoot formation was initiated from crown explant cultured on MS media with the addition of 2 mgL-1 of growth regulator Benzyladenine (BA).  Whereas for shoot multiplication, 3 mgL-1 of BA was evidently effective with the average shoot number was 126 shoots per explant. The application of high BA concentration (3-5 mgL-1) in multiplication media was capable of inducing more shoots, but the shoots resulted were shorter. In contrast, multiplication media supplemented with low BA concentration (1-2 mgL-1) yielded less shoots, but the shoots were longer. Plantlet regeneration was accomplished by inducing roots in the shoots yielded on MS media containing 1 mgL-1 growth regulator NAA. In vitro plants of vetiver had been successfully grown on soil media under greenhouse condition. By using foregoing method, it is possible to conduct mass propagation of vetiver through tissue culture technique.
Struktur dan Perkecambahan Serbuk Sari Pada Porang (Amorphophallus Muelleri Blume) Dwi Gusmalawati; Serafinah Indriyani; Rodiyati Azrianingsih; Zainal Abidin
G-Tech: Jurnal Teknologi Terapan Vol 7 No 2 (2023): G-Tech, Vol. 7 No. 2 April 2023
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.171 KB) | DOI: 10.33379/gtech.v7i2.2449

Abstract

This study aims to analyze the structure and germination of pollen in porang. Pollen structure and germination were observed microscopically. Pollen germination observations were carried out three days before anthesis (M-3), two days before anthesis (M-2), one day before anthesis (M-1), anthesis (M) to four days after anthesis (M+4). Pollen in porang has a dimorphic structure (X and Y structures: monad and compound units). Structure X with axis length (P)= 30-50 µm, equatorial diameter (E)= 16.67-41.67 µm, and index P/E= 1.19-1.79, while structure Y with P= 50-84 µm, E= 34-67 µm, and P/E index= 1.25-1.40. Porang pollen was subspheroidal and prolate in shape; the length of the axes of the media and magna types; monocolpate aperture type. The highest viability of the X structure was at M (31.58%), while the Y structure was at M+1 (21.76%). M+3 viability begins to decline, so porang artificial pollination is appropriate when pollen viability is high (M or not more than M+3).