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All Journal INDONESIAN JOURNAL OF ESSENTIAL OIL
Edi Priyo Utomo
Universitas Brawijaya
Agriculture, Biological Sciences & Forestry

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Clonal Propagation of Vetiveria zizanioides L. through Tissue Culture Technique Wahyu Widoretno; Arbaul Fauziah; Serafinah Indriyani; Edi Priyo Utomo
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 2, No 1 (2017)
Publisher : Institut Atsiri Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.814 KB) | DOI: 10.21776/ub.ijeo.2017.002.01.05

Abstract

 In vitro propagation method for vetiver (Vetiveria zizanioides L.) had been effectively developed in this study. Several variations of media were used for shoot initiation and multiplication. Shoot formation was initiated from crown explant cultured on MS media with the addition of 2 mgL-1 of growth regulator Benzyladenine (BA).  Whereas for shoot multiplication, 3 mgL-1 of BA was evidently effective with the average shoot number was 126 shoots per explant. The application of high BA concentration (3-5 mgL-1) in multiplication media was capable of inducing more shoots, but the shoots resulted were shorter. In contrast, multiplication media supplemented with low BA concentration (1-2 mgL-1) yielded less shoots, but the shoots were longer. Plantlet regeneration was accomplished by inducing roots in the shoots yielded on MS media containing 1 mgL-1 growth regulator NAA. In vitro plants of vetiver had been successfully grown on soil media under greenhouse condition. By using foregoing method, it is possible to conduct mass propagation of vetiver through tissue culture technique.
Studi Hubungan Aktivitas Struktur Terhadap Penentuan Karakteristik Aroma Senyawa melalui Pengujian Molecular Docking Agung Nurfauzy; Edi Priyo Utomo; Elvina Dhiaul Iftitah
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 2, No 1 (2017)
Publisher : Institut Atsiri Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (815.703 KB) | DOI: 10.21776/ub.ijeo.2017.002.01.02

Abstract

Deskripsi karakteristik aroma suatu senyawa hingga saat ini masih mengandalkan subjektivitas panelis manusia yang pengujiannya dapat dipengaruhi oleh pengalaman dan persepsi lain. Pada penelitian ini dilakukan pengembangan pengujian melalui metode molecular docking menggunakan 18 senyawa beraroma (standar) yang diujikan pada reseptor olfaktori manusia OR1G1 dan interaksinya dibandingkan dengan deskripsi standar karakteristik aroma dari masing-masing senyawa. Hasil menunjukkan bahwa, senyawa dengan karakter aroma yang sama akan berinteraksi pada posisi urutan asam amino (sequence) reseptor cenderung konsisten, sehingga dapat diperoleh pemetaan sisi aktif reseptor yang merepresentasikan aroma tertentu. Berdasarkan hal tersebut, pengujian senyawa 2,7-dimetil-8-oksooktan-2-il asetat yang belum memiliki deskripsi standar aroma dapat diprediksi kemiripan karakter aromanya dilihat dari sequence asam amino yang berinteraksi dan pendekatan statistik hasil interaksi senyawa yang dibandingkan dengan senyawa standar. Molecular docking menunjukkan bahwa hubungan struktur dan aroma (Structure-Odor Relationship = SOR) dapat dijadikan acuan untuk menjelaskan karakter aroma serta prediksi aroma senyawa tertentu yang lebih bersifat objektif dan tidak bergantung pada persepsi panelis.
Co-Authors Agung Nurfauzy Arbaul Fauziah Elvina Dhiaul Iftitah Serafinah Indriyani Wahyu Widoretno