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Metabolit Sekunder Sekresi Jamur Penicillium spp. Isolat Tanah Gambut Riau sebagai Antijamur Candida albicans Yuana Nurulita; Yuharmen Yuharmen; Nuryani Nenci; Ayu Octa Mellani; Titania Tjandarawati Nugroho
Chimica et Natura Acta Vol 8, No 3 (2020)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v8.n3.32452

Abstract

Mikroba dan tanaman merupakan sumber metabolit sekunder yang aktif dieksplorasi potensinya. Laboratorium Enzim, Fermentasi dan Biomolekuler Jurusan Kimia FMIPA Universitas Riau telah mengisolasi beberapa jenis mikroba khususnya jamur dari tanah gambut Hutan Primer Cagar Biosfer Giam Siak Kecil Bukit Batu (GSKBB) Provinsi Riau. Beberapa isolat tersebut adalah jamur Penicillium sp. LBKURCC29 dan LBKURCC30 yang memiliki potensi menghasilkan enzim selulase. Tujuan dari penelitian ini adalah untuk mengeksplorasi potensi lain dari kedua jamur isolat lokat tanah gambut Riau tersebut dalam hal ini potensi sebagai sumber antimikroba, antijamur Candida albicans. Produksi metabolit sekunder antijamur dilakukan dengan cara fermentasi batch dalam media cair yang diinokulasi dengan spora jamur (7×1012 spora untuk 50 mL media) selama 14 hari dengan bantuan rotary shaker kemudian disaring dengan menggunakan kertas saring GF/C. Filtrat kemudian diekstraksi menggunakan etil asetat. Ekstrak etil asetat kemudian diuapkan dan dipekatkan menggunakan rotary evaporator. Ekstrak kasar etil asetat kemudian dilarutkan dengan metanol untuk diuji lebih lanjut. Pada penelitian ini dilakukan karakterisasi ekstrak dengan uji fitokimia, KLT, dan HPLC, serta uji antijamur C. albicans dengan metode difusi cakram. Hasil uji fitokimia menunjukkan bahwa masing-masing ekstrak Penicillium sp. LBKURCC29 dan LBKURCC30 mengandung senyawa terpenoid. Karakterisasi lebih lanjut ekstrak kasar etil asetat diuji KLT dan HPLC menunjukkan pola noda dan puncak yang berbeda. Untuk uji antijamur, hanya ekstrak Penicillium sp. LBKURCC29 yang memiliki aktivitas antijamur terhadap jamur C.albicans pada konsentrasi 5,7 mg/mL dengan diameter zona hambat sebesar 8,05 nm yang merupakan penghambatan 33,78% dibandingkan dengan kontrol positif Ketokonazol® (23,83 mm).
AKTIVITAS ANTIBAKTERIA EKSTRAK METANOL DAUN TANAMAN Solanum ferox L Hazimah Hazimah; Zefri Azharman; Yuharmen Yuharmen; Virsa Rahyuti; Afriliani Afriliani
JURNAL REKAYASA SISTEM INDUSTRI Vol 4 No 1 (2018): (November 2018)
Publisher : Universitas Putera Batam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (640.368 KB)

Abstract

Solanum ferox or acid aubergine plants is one type of vegetable used as flavoring in food. Solanum ferox contains terpenoids, steroids, flavonoids, alkaloids and phenolics. Solanum ferox has antipyretic, antirheumatic, anti-asthma, antiviral and as a drug for syphilis. The purpose of this study is to test the antibacterial activity of leaf methanol extract of Solanum ferox plant. Leaf Solanum ferox is extracted using methanol. Methanol extract was tested for antibacterial activity using diffusion method to use E.coli, S.aureus and B.subtilis bacteria. Amoxicillin is used as a positive standard while negative controls are solvents used to dissolve samples. Antibacterial activity of methanol and amoxicillin extract showed inhibitory zones of E.coli of 7,72-11,67 mm and 19.97 mm, inhibitory zone to bacterium B.subtilis only at 5.7 μg/mL concentration of 11,29 mm and amoxicillin 18, 51 mm.
Synthesis of Silver Nanoparticles from Cucumis melo L. and Assessment of Its Antimicrobial Properties Yuli Haryani; Iswatun Nabella; Yuharmen Yuharmen; Ganis F. Kartika
Pharmacology and Clinical Pharmacy Research Vol 3, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (838.497 KB) | DOI: 10.15416/pcpr.v3i2.18109

Abstract

Over the past decade, the use of biological agents such as plants, cyanobacteria, bacteria, and fungi for synthesis of metal nanoparticles has been developed. The aim of this study was to investigate antibacterial activity of Cucumis melo L. peel extract and its nanoparticles formulation against Eschericia coli. The nanoparticles were made using silver nitrate with the ratio between C. melo L. extract and silver nitrate aqueous solution (1 mM) were 1:10 and 1:15. The formation of silver nanoparticles was observed after microwaved for 30, 60, 90, 120, 150, and 180 seconds by visible spectrophotometry analysis. Phytochemical screening revealed the presence of flavonoid and terpenoid within the extract. However, the characteristic of surface plasmon resonance band, which occurs in the range of 410-500 nm were not found in the nanoparticle extract, even though the reaction time was extended to 330s. Antibacterial activity against E. coli of the extract and its nanoparticle formulations was determined using Resazurin microtiter assay and compared to Amoxsan® as positive control. The highest E. coli inhibition was exhibited by the nanoparticles (79.8739±0.3859), followed by the extract (65.2821±0.9949). The nanoparticles and the extract have potent antibacterial activity compared to positive control (84.5519 ± 0.2544). In conclusion, the antibacterial activity of the C. melo L. silver nanoparticles formulation was better than its extract.Keywords: Cucumis melo, silver nanoparticles, UV-Vis spectra