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Aniversari Apriana
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
Agriculture, Biological Sciences & Forestry

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Identifikasi Perubahan Karakter Agronomis Padi Transgenik Penanda Aktivasi cv. Asemandi Generasi T1 Atmitri Sisharmini; Aniversari Apriana; Diah Nurmaliki; Tri Joko Santoso; Kurniawan R. Trijatmiko
Jurnal AgroBiogen Vol 9, No 3 (2013): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n3.2013.p107-116

Abstract

The activation-tagged populations of transgenic rice cv.Asemandi have been developed by introducing construct ofactivation taq Ac/Ds into rice genom of cv. Asemandi. The T1transgenic rice populations cv. Asemandi containingconstruct of activation taq have been obtained and neededto be characterized. This study aimed to identify the Bastaherbicide resistant plants, transgenic plants containing hptand bar genes, and changes in agronomic traits. Bastaresistant plant was identified by treating leaf with bastasolution. Hpt and bar genes were detected by PCR usingspecific primers. Phenotype characters were identified byobserving and measuring their agronomic parameters. Thestudy results showed that out of 315 rice transgenic cv.Asemandi T1 treated with Basta solution, 176 (55.87%) plantswere indicated to be resistant to Basta. The results of PCRanalysis revealed that eight rice transgenic cv. Asemanditested contained both hpt and bar genes. In general,compared to the nontransgenic plants, there were changesin several agronomic parameters of T1 transgenic plants cv.Asemandi, including plant height, days to flowering, days toharvesting, periods of grain filling, and weight of 100 grains.Correlation analysis showed that there was no correlationbetween days of harvesting to weight of 100 grains intransgenic rice, but there was correlation in nontransgenicrice. Transgenic rice plants cv. Asemandi with changes inthe agronomic characters will be useful for further study,such as to analyze the function of the genes.
Identifikasi Galur dan Gen-gen Terkait Toleran Kekeringan pada Padi Transgenik cv. T309 yang Mengandung Vektor Penanda Aktivasi Tri Joko Santoso; Aniversari Apriana; Atmitri Sisharmini; Kurniawan R. Trijatmiko
Jurnal AgroBiogen Vol 9, No 3 (2013): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n3.2013.p97-106

Abstract

Activation tagging is an efficient tool forfunctional analysis of the rice genes. We have developed anumber of transgenic rice lines (Oryza sativa L. ssp.japonica cv. Taipei 309) containing activation tagging vector.However, the phenotypes and genotypes of these lines, inrelation to the drought stress, have not been analyzed. Theobjectives of this research were to identify transgenic ricelines that showed tolerance to the drought stress and toidentify the genes that may be associated with the droughtstress. The drought stress tolerance in transgenic rice lineswas identified by testing their tolerance to the drought stressand also by detecting the presence of bar and nptII genes.The result showed that 56 out of 59 rice lines were resistantto Basta herbicide and three of them showed tolerance todrought stress, namely PA.T-1.2, PA.T-4.1, and PA.T-5.1 lines.PCR analysis showed that PA.T-1.2 and PA.T-4.1 containedboth hptII and bar genes, while the PA.T-5.1 line containedbar gene only. Thermal Asymetric Interlaced-PCR (TAILPCR)analysis showed that two genes may be asssociatedwith the drought stress tolerance. Those genes areOSJNBa0004120.14 that produces uridylate putative kinaseand OsPPCK2L that produces phosphoenolpyruvatecarboxylase kinase.
Introduksi Konstruk Over-Ekspresi Kandidat Gen OsWRKY76 melalui Agrobacterium tumefaciens pada Tanaman Padi Nipponbare Aniversari Apriana; Atmitri Sisharmini; Wening Enggarini; Sudarsono Sudarsono; Nurul Khumaida; Kurniawan Rudi Trijatmiko
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p19-27

Abstract

Delivering of Over-Expression Construct OsWRKY76Candidate Gene in Rice cv. Nipponbare throughAgrobacterium tumefaciens. Aniversari Apriana, AtmitriSisharmini, Wening Enggarini, Sudarsono, Nurul.Khumaida, and Kurniawan R. Trijatmiko. Plant geneticimprovement can be done through classical breeding orgenetic engineering. WRKY is a transcription factor involvedin regulating plant defense responses. OsWRKY76 gene islocated in a narrow segment of chromosome 9 which isidentified previously to be related to wide spectrumresistance in rice. A sequence of OsWRKY76 (+1.200 bp)has available in the gene bank and it makes possible toisolate, clone, and construct the gene into over-expressionvector. The aim of this research was to assemble an overexpressionconstruct of OsWRKY76 candidate gene andintroduce it into rice through Agrobacterium-mediatedtransformation. A construct of pCAMBIA-1301::35S::OsWRKY76 has been successfully assembled andtransformed into embryogenic calli of rice cv. Nipponbareusing A. tumefaciens strain Agl-1 and EHA 105. A number of126 independent lines has been produced, in which Agl-1showed 3.8 times more efficient than EHA 105. PCR analysisof randomly selected 25 independent lines showed that allof them positively contained hptII gene, a selectable markerused in the over-expression construct of the OsWRKY76candidate gene. Based on the result, it could be concludedthat the over-expression construct of OsWRKY76 candidategene have been successfully introduced into the tissue ofNipponbare.
Co-Authors Atmitri Sisharmini Diah Nurmaliki Kurniawan R. Trijatmiko Kurniawan Rudi Trijatmiko Nurul Khumaida Sudarsono Sudarsono Tri Joko Santoso Wening Enggarini