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Annisaa Yusrina
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Identifikasi Morfometrik Sperma Domba Lokal sebagai Dasar Aplikasi Sexing Sperma Nurcholidah Solihati; Siti Darodjah Rasad; Annisaa Yusrina; Yosep Indra Dimyati
Jurnal Ilmu Ternak Vol 17, No 2 (2017)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.368 KB) | DOI: 10.24198/jit.v17i2.15138

Abstract

Penelitian ini bertujuan untuk mengidentifikasi ukuran-ukuran sperma berdasarkan metode morfometrik sebagai dasar aplikasi sexing sperma pada Domba Lokal. Penelitian ini dilakukan dengan menggunakan 20 ekor Domba Lokal yang ditampung semennya dan dibuat preparat differensial. Pengamatan morfometrik sperma dilakukan di bawah mikroskop pembesaran 10x40 menggunakan aplikasi DP2BSW. Parameter yang diamati meliputi ukuran panjang, lebar dan luas kepala sperma serta panjang ekor sperma. Pengukuran dilakukan terhadap 70 sd 200 sel sperma untuk setiap parameter. Data yang diperoleh dihitung nilai minimum, maksimum dan standar deviasi dari setiap parameter. Hasil penelitian menunjukkan bahwa kepala sperma memiliki rataan panjang minimum 6,85±0,40 μm dan maksimum 10,27± 0,51μm; rataan lebar kepala sperma minimum 3,76±0,43 μm dan maksimum 6,71±0,43 μm; rataan luas kepala sperma minimum 23,28±2,90 μm2 dan maksimum 59,30±4,33 μm2; rataan panjang ekor sperma minimum 36,74±6,73 μm dan maksimum 69,53±3,42 μm. Berdasarkan hasil-hasil pengukuran tersebut, diketahui bahwa sperma Domba Lokal memiliki ukuran minimum dan maksimum yang selanjutnya dapat diidentifikasi sperma pembawa kromosom X dan Y serta proporsinya. Disimpulkan bahwa hasil morfometrik sperma Domba Lokal dapat dijadikan dasar untuk sexing sperma pada Domba Lokal. Kata kunci:  panjang, lebar, luas kepala sperma, panjang ekor 
Quality and Longevity of Local Ram’s Sexed Sperm with Albumin Colums Nurcholidah Solihati; Siti Darodjah Rasad; Annisaa Yusrina; Kikin Winangun; Toha .
Jurnal Ilmu Ternak Vol 19, No 2 (2019): December
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.025 KB) | DOI: 10.24198/jit.v19i2.25632

Abstract

Sperm sexing applications can support the effectiveness of artificial insemination (AI) programs in sheep. The albumin column method is one method of sperm sexing that is relatively easy and inexpensive compared to other methods, but research needs to be done to obtain a method that results in the quality and longevity of sexed sperm that is feasible to use for AI programs, one of which is by searching for incubation or sexing time. This study used 10 ejaculate semen from two local sheep around 2-3 years old. We used bovine serume albumin (BSA) as the albumin column with two fractions which is 5% in the upper fraction and 10% in the lower fraction. The treatment given was three lengths of incubation/sexing time (45, 60 and 75 minutes) which were repeated 10 times. Parameters consist of the quality of semen (motility, abnormalities, intact plasma membrane, intact acrosome cup) and longevity. Data were analyzed by analysis of variance and Duncan's advanced test. The results showed that the length of sexing time had a very significant effect on the quality of semen, but the length of incubation/sexing time up to 75 minutes still produced the quality of semen that was qualify for AI. The result also showed that incubation/sexing time does not affect the longevity of sexed sperm. It was concluded that the quality of sexed sperm of local ram that sexing with albumin colums fulfilled the quality for artificial insemination and the incubation/sexing time for 45-75 minutes can be used to obtain the qualify sexed sperm for artificial insemination with chilled semen.
Co-Authors Kikin Winangun Nurcholidah Solihati Nurcholidah Solihati Siti Darodjah Rasad Toha . Yosep Indra Dimyati