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All Journal Jurnal Ilmu Ternak Kandaga - Media Publikasi Ilmiah Jabatan Fungsional Tenaga Kependidikan
Kikin Winangun
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Library & Information Science Public Health Veterinary Other

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Quality and Longevity of Local Ram’s Sexed Sperm with Albumin Colums Nurcholidah Solihati; Siti Darodjah Rasad; Annisaa Yusrina; Kikin Winangun; Toha .
Jurnal Ilmu Ternak Vol 19, No 2 (2019): December
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.025 KB) | DOI: 10.24198/jit.v19i2.25632

Abstract

Sperm sexing applications can support the effectiveness of artificial insemination (AI) programs in sheep. The albumin column method is one method of sperm sexing that is relatively easy and inexpensive compared to other methods, but research needs to be done to obtain a method that results in the quality and longevity of sexed sperm that is feasible to use for AI programs, one of which is by searching for incubation or sexing time. This study used 10 ejaculate semen from two local sheep around 2-3 years old. We used bovine serume albumin (BSA) as the albumin column with two fractions which is 5% in the upper fraction and 10% in the lower fraction. The treatment given was three lengths of incubation/sexing time (45, 60 and 75 minutes) which were repeated 10 times. Parameters consist of the quality of semen (motility, abnormalities, intact plasma membrane, intact acrosome cup) and longevity. Data were analyzed by analysis of variance and Duncan's advanced test. The results showed that the length of sexing time had a very significant effect on the quality of semen, but the length of incubation/sexing time up to 75 minutes still produced the quality of semen that was qualify for AI. The result also showed that incubation/sexing time does not affect the longevity of sexed sperm. It was concluded that the quality of sexed sperm of local ram that sexing with albumin colums fulfilled the quality for artificial insemination and the incubation/sexing time for 45-75 minutes can be used to obtain the qualify sexed sperm for artificial insemination with chilled semen.
KUALITAS LARUTAN PENGENCER DAN KUALITAS SEMEN DOMBA PADA TEMPERATUR PENYIMPANAN YANG BERBEDA Kikin Winangun
Kandaga– Media Publikasi Ilmiah Jabatan Fungsional Tenaga Kependidikan Vol 1, No 1 (2019)
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.404 KB) | DOI: 10.24198/kandaga.v1i1.21217

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh temperatur penyimpanan terhadap kualitas larutan pengencer dan kualitas semen domba. Materi yang digunakan dalam penelitian ini adalah larutan tris kuning telur dan semen segar domba lokal. Data dianalisis menggunakan Anova dan di uji lanjut menggunakan uji Duncan. Larutan tris kuning telur disimpan pada temperatur yang berbeda-beda yaitu penyimpanan pada temperatur 7oC, 28oC dan  37 oC. Variabel yang diukur pada penelitian ini yaitu kualitas larutan seperti berat jenis, viskositas, pH larutan, dan kualitas semen berupa motilitas dan viabilitas spermatozoa. Hasil penelitian menunjukkan bahwa temperatur penyimpanan berpengaruh nyata (P<0,05) terhadap perubahan kualitas pengencer dan kualitas semen domba. Hasil penelitian ini dapat disimpulkan bahwa penyimpanan larutan pengencer pada temperatur 7°C menghasilkan kualitas pengencer tris kuning telur dan kualitas semen domba yang paling baik dibandingkan dengan teknik penyimpanan lainnya. Teknik penyimpanan pengencer pada temperatur 7°C memiliki nilai tinggi pada berat jenis (1,0069 (g/ml)), viskositas (2,8537 (mm2/s)/(cSt)) dan pH (6,5) , selain itu sperma mampu bertahan selama 13 hari.
Co-Authors Annisaa Yusrina Nurcholidah Solihati Siti Darodjah Rasad Toha .