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All Journal Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat
Nurliani Bermawie
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RESPON TANAMAN LADA (PIPER NIGRUML) VARIETAS CIINTEN TERHADAP IRADIASI SINAR GAMMA / Respons of Gamma Irradiation on Black Pepper (Piper nigrum L.) Ciinten Variety Nur Laela Wahyuni Meilawati; Nurliani Bermawie; Agus Purwito; Dyah Manohara
Jurnal Penelitian Tanaman Industri Vol 22, No 2 (2016): Juni, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n2.2016.71-80

Abstract

Pepper is an introduced species and has always been propagated vegetatively, so it has narrow genetic base. High genetic diversity is necessary to produce new varieties, especially for breeding of resistance to foot rot disease. Increasing genetic diversity can be done through gamma ray irradiation. This research aims to evaluate response of black pepper Ciinten variety at seed and radicle emergence phases to gamma ray irradiation. The research was conducted in PAIR BATAN and greenhous e IMACRI from June 2014 to April 2015. The plant material was Ciinten variety at the seed and radicle emergence phases. The experimental design used was completely randomized design (CRD) with one factor which is dose of irradiation with seven levels (0, 25, 50, 75, 100, 125, 150) Gy. Each treatment consisted of three replications, each replication consisted of 60 seeds. Both phases showed significant differences in perfomances between dose in plant height, leaf length, number of leave, number of internode. Radiosensitivity of pepper on radicle emergence phase was higher than the seed phase indicated by LD50 (Lethal Dose 50). LD50 at seed phase was 68.15 Gy, whereas LD50 of the radicle emergence phase was 30 Gy. The higher irradiation dose that given to both treatment phases caused reduction in plant height, leaf length, while the number of leaves and nodes decreasing. Irradiation dose 25 dan 50 Gy in seed phase and 25 Gy in radicle emergence phase significantly increase genetic diversity base on quantitative, qualitative characters, anatomy and leaf resistence to P. capsici infection. Eighteen putative mutants resistant to infection P. capsici. Eighteen mutant putative Ciinten pepper varieties were expected to be high yielding varieties with more research in the greenhouse and in the field to determine the production and quality potential.
PENGARUH PENGENCERAN MEDIA DASAR TERHADAP PERTUMBUHAN KULTUR JAHE DALAM PENYIMPANAN SECARA IN VITRO SITTI FATIMAH SYAHID; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 5, No 4 (2000): Maret, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n4.2000.115-118

Abstract

Effect of dilution of basic medium on ginger growth during conservation in vitroBasic medium dilution could affect the growth of the culture. The effect of basic medium dilution was evaluated at the laboratory of Research Institute for Spices and Medicinal Crops from October 1998 to June 1999. Sterile shoots of ginger were used as ex plants. Basic medium dilution from 3/4 MS, 1/2 MS, 1/4 MS and MS normal (control) were used as treatments. The media were enriched with BA 3 mg/1 and Sucrose 6%. The treatments wee arranged in a completely randomized design with three eplications. Results showed that all the dilution treatments inhibited the grow of the culture during live months of conservation period, especially on 1/4 MS + BA 3 mg/1 + Sucrose 6% . On the media, the number of shoots wee 3.55. Based on cultue performance the best medium for conservation was 1/2 MS + BA 3 mg/1 + Sucrose 6%. All of the culture was able to growth in regeneration media after they were conserved for ive months. Results of this experiment could be applied in reducing sub-cultue frequency to a new media and maintain the culture stability. e
KONSERVASI TANAMAN LADA (Piper nigrum L.) SECARA IN VITRO YELNITITIS YELNITITIS; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 7, No 3 (2001): September, 2001
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v7n3.2001.88-92

Abstract

In vitro conservation of black pepper (Piper nigrum L.)Black pepper (Piper nigrum L.) is one of the economically im¬ potant spices. The major constraint in black pepper cultivation and conservation in ield is foot rot disease caused by Phytopthora capsici which could cause plants die. Conservation of black pepper germplasms as living collections in ield is risky due to pests and natural disaster. The experiment on in vitro cop ervation of black pepper var. LDL was conducted al the laboratory of Plant Genetic Resources and Breeding, Research Institute for Spice and Medicinal Crops (RISMC) Bogor from April 1998 to Maret 1999. Single node cuttings from sterile culture were used as explains. The explains were cultured on Murashige and Skoog (MS) medium on full and half strength concentration supplemented with paclobutrazol (paclo) (0, 1, 3 and 5 mg/1). The experiment was performed in a randomized complete block design arranged factorially with 10 replications. The result showed that the medium supplemented with paclo on both full MS and MS A medium could suppress vegetative growth until 12 months. There was no signiicant interaction between medium and paclo on shoot initiation. The effect was signiicant on shoot height, number of leaves and culture performances. Increasing paclo concen¬ tration caused higher suppression of plant growth. MS A medium supplemented with paclo 5 mg/1 showed the slowest growth with shool height 2.10 cm and number of leaves 9. Culture performance was fresh, with green leaves and vigorous. Advcntive shoots were able to regenerate on the medium supplemented with BA 0.3 mg/1. In vitro conservation of black pepper with paclo did not change plant regeneration ability. Therefore, this technique may be used as an altenative method for black pepper conservation.
OBSERVASI HASIL DAN MUTU LADA LOKAL DI DUA AGROEKOLOGI Nurliani Bermawie; Sri Wahyuni; Ruby Heryanto; Rudi T Setiyono; Laba Udarno
Buletin Penelitian Tanaman Rempah dan Obat Vol 24, No 2 (2013): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v24n2.2013.%p

Abstract

Upaya peningkatan produktivitas lada dapat dilakukan dengan penggunaan bahan tanaman unggul lokal. Tujuan dari penelitian ini adalah untuk mengetahui potensi hasil, komponen hasil dan mutu lada lokal asal Sukabumi dan varietas pembanding Petaling-1, di dua agroekologi berbeda, di kabupaten Sukabumi dan kabupaten Purwakarta. Pengamatan dilakukan pada 10% tanaman contoh dari tiap populasi, pada sifat hasil per pohon, komponen hasil dan mutu. Data dianalisis dengan uji t. Pertumbuhan, hasil, komponen hasil dan mutu lada kedua varietas secara umum lebih baik di Purwakarta dari pada di Sukabumi. Hasil uji t memperlihatkan bahwa hasil per pohon, karakter jumlah malai per tanaman, panjang malai, jumlah biji per malai, bobot malai dan panjang tangkai malai berbeda antara varietas lokal dengan varietas Petaling-1, dan lada lokal lebih baik dari Petaling-1. Hasil lada lokal per pohon di Purwakarta 2,79 kg, dan varietas Petaling-1, yaitu1,67 kg per pohon. Di Sukabumi hasil buah segar lada lokal juga lebih tinggi dari varietas Petaling-1 berturut-turut 2,13 kg dan 1,30 kg per pohon. Malai lada lokal 9-13,5 cm, dengan jumlah buah per malai 70-140 butir, lebih panjang dan lebih banyak dari malai Petaling-1 berturut-turut 5-9 cm, dan 20-60 butir. Mutu lada putih maupun lada hitam berbeda antar lokasi, dan mutu di Purwakarta lebih baik dari pada di Sukabumi. Di kedua lokasi, mutu lada lokal baik kadar minyak atsiri, oleoresin maupun piperin lebih baik dari Petaling-1. Bobot buah segar lada lokal sama dengan Petaling-1 sehingga cocok untuk diproses menjadi lada putih. 
Co-Authors Agus Purwito Cheppy Syukur Dyah Manohara Laba Udarno MELATI MELATI NATALINI NOVA KRISTINA nFN Sukarman NUR LAELA W. MEILAWATI Nur Laela Wahyuni Meilawati Ruby Heryanto Rudi T Setiyono S. PURWIYANTI SITTI FATIMAH SYAHID Sri Wahyuni YELNITITIS YELNITITIS