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Ellina Mansyah
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Agriculture, Biological Sciences & Forestry Education

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Analisis Variabilitas Genetik Manggis (Garcinia Mangostana L.) Di Jawa dan Sumatera Barat Menggunakan Teknik RAPD Ellina Mansyah; Achmad Baihaki; Ridwan Setiamihardja; Juliati S. Darsa; , Sobir
Zuriat Vol 14, No 1 (2003)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v14i1.6813

Abstract

Informasi tentang variabilitas genetic suatu tanaman penting untuk program pemuliaan karena merupakan dasar untuk pengembangan tanaman tersebut selanjutnya. Sampai saat ini diketahui secara luas bahwa tanaman manggis tidak memiliki variabilitas secara genetic karena mempunyai mekanisme reproduksi secara apomiksis. Penelitian ini bertujuan untuk mengetahui variabilitas genotip manggis pada berbagai lingkungan tumbuh di Jawa dan Sumatera Barat melalui teknik RAPD (Random Amplified Polymorphic DNA). Observasi genetic dilaksanakan dengan menggunakan 23 sampel DNA yang mewakili populasi manggis dari berbagai lokasi tersebut. Lima praimer acak digunakan dalam analisis RAPD dan diperoleh 51 pita DNA yang terdiri dari 42 pita polimorfik dan 9 pita monomorfik. Pembuatan dendogram karakter genotip dilakukan dengan bantuan program NTSYSpc versi 2.1. Hasil penelitian menunjukkan bahwa tanaman manggis bervariasi secara genetik. Variasi genetiknya adalah sebesar 56.6% dengan koefisien kemiripan Dice 0.73–1.00. Koefisien korelasi antar pita DNA cukup tinggi yaitu r = 0.896, yang berarti dendogram berdasarkan pola pita DNA sangat baik.
Population Dynamic of Scirtothrips dorsalis Hood (Thysanopetera: Thripidae) on Mango and Associated Weeds Under Low and Intensive Agricultural Practices Affandi Affandi; Celia dela Rosa Medina; Luis Rey Ibanez Velasco; Pio Arestado Javier; Dinah Pura Tonelete Depositario; Ellina Mansyah; Hardiyanto Hardiyanto
AGRIVITA, Journal of Agricultural Science Vol 41, No 3 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i3.2316

Abstract

Population dynamic information and its influence factors are basic need for the best insect strategic control. This research objective was to descript and compare thrips S. dorsalis population dynamic on mango and associated weeds under low and intensive cultural practices. Reseacrh was conducted in PT. Trigatra Rajasa mango plantation in Ketowan, Arjasa, Situbondo, East Java, Indonesia from February 2014 to January 2015. The investigation was done through observation of S. dorsalis number associate on each mango growth stages and weeds under the canopy of mango. Arithmetic and descriptive method were applied to ensure the population oscillations pattern among phenological stages of mango and weeds under mango tree canopy. Result showed that population fluctuation of S. dorsalis was determined by growth stages of mango trees and the availability of initial built up of population. Flush growth stage was the most preferred stage which had high input agricultural practices such as fertilizer and tree conditioning and was supported for more numbers of thrips. Weeds Desmanthus leptophyllus, Achalypha indica, Azadirachta indica and Tephrosia vogelii were functioning as breeding habitat for early built-up population on mango trees. Climate factors especially rainfall was also affected population fluctuation of S. dorsalis on mango trees and weeds.
Co-Authors Achmad Baihaki Affandi Affandi Celia dela Rosa Medina Dinah Pura Tonelete Depositario Hardiyanto Hardiyanto Juliati S. Darsa Luis Rey Ibanez Velasco Pio Arestado Javier Ridwan Setiamihardja Sobir Sobir