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Marjam Toding
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Agriculture, Biological Sciences & Forestry Education

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Pemanfaatan marka gen OsIRT1 untuk seleksi galur populasi silang ganda turunan padi lokal Markuti toleran cekaman keracunan Fe Marjam Toding; Dwinita W. Utami
Zuriat Vol 23, No 1 (2012)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v23i1.6866

Abstract

Keracunan Fe adalah salah satu cekaman abiotik yang dapat secara nyata mempengaruhi produksi padi, terutama pada lahan basah marginal. Keracunan Fe dapat menurunkan produksi padi 60% hingga 90% pada daerah podzolik merah kuning. Mekanisme toleransi keracunan Fe pada tanaman padi salah satunya diperankan gen OsIRT1 (O. sativa Regulated Transporter). Peta genetik dari gen ini terdapat pada kromosom 7, pada posisi 47,787-52,212 Mb, yaitu memanjang pada contig AP004297 dan lokus MSU Osa1 :  LOC_Os07g01090. Salah satu aksesi plasma nutfah padi lokal tepilih berdasarkan hasil penelitian dengan pendekatan allele mining adalah Markuti(Indica, IRN:) yang diketahui memiliki alel gen OsIRT. Plasma nutfah tersebut telah digunakan sebagai material genetik persilangan ganda dengan varietas terseleksi Bio110 (IR54/Parekaligolara//Bio110/Markuti), dalam program pembentukan galur-galur harapan padi baru toleran cekaman biotik (tahan penyakit BLB dan Blas) dan abiotik (toleran keracunan Fe dan kahat P). Tujuan dari penelitian ini adalah: menguji keragaan sifat ketahanan galur-galur populasi F2 hasil silang ganda (IR54/Parekaligolara//Bio110/Markuti) terhadap cekaman keracunan Fe, berdasarkan pengujian phenotyping di rumah kaca dan genotyping menggunakan marka molekular spesifik penanda gen OsIRT1. Uji keragaan fenotipe dilakukan dengan menguji respon 88 total tanaman F2 yang ditanam dengan kondisi Fe tinggi (tanah masam-tergenang). Sedangkan keragaan genotipe dilakukan dengan menganalisis PCR populasi di atas menggunakan marka spesifik penanda  gen OsIRT1. Hasil pengujian menunjukkan bahwa keragaan galur-galur F2 hasil silang ganda (IR54/Parekaligolara//Bio110/Markuti) terdapat 68 tanaman bersifat toleran (skor 2-3), 11 tanaman bersifat sedang (skor 5) dan 9 tanaman bersifat rentan (skor 7). Sedangkan keragaan genotipe berdasarkan analisis PCR menggunakan marka OsIRT1 menunjukkan segregasi dengan tipe: 0,1 dan 2 yang berturut-turut co-segregasi dengan sifat : peka, sedang dan toleran.   
PERTUMBUHAN DAN PRODUKSI TANAMAN PADI DENGAN METODE SRI (SYSTEM OF RICE INTENSIFICATION) PADA BERBAGAI UMUR PINDAH TANAM BIBIT Rifaine M.N. Erungan; Johannes E.X. Rogi; Marjam Toding; Yefta Pamandungan
COCOS Vol. 6 No. 10 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v6i10.8233

Abstract

ABSTRACT   This research aims to determine the effect of age on the growth and transplanting rice production by using the system of planting methods SRI ( System of Rice Intencification ) carried out in the village of Sulu District of Tatapaan for 4 months from May to September 2014. The research used randomized block design with treatment age transplanting U1 ( 0 HSS ) , U2 ( 10 HSS ) , U3 ( 15 HSS ) . Data were analyzed using the F test and continued with Least Significant Difference test level of 5 % .  Broadcaster age transplanting significant effect on plant height and number of tillers , and no real effect on the number of productive tillers , dry grain harvest weight , 1000 grain weight , the weight of milled rice per plot , weight of dry milled grain per plant , and productivity .  The treatment gives the best results in the production of which is the U3 treatment with seedling transplanting age 15 HSS . Keywoards : transplanting age, SRI method, rice
KARAKTERISASI TANAMAN JAGUNG UNGU F1 HASIL BERSARI BEBAS JAGUNG MANADO KUNING DENGAN JAGUNG UNGU Oksandi Darius Tumei; Marjam Toding; Yefta Pamandungan
COCOS Vol. 10 No. 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v1i4.22097

Abstract

ABSTRACT  This research aims to study the characteristic of the growth components of purple cornF1resulted from open pollinated between Yellow Manadocornandpurplecorn. Research carried out invillages ofKalasey 2,Mandolangsub-district,Minahasa regency, Province of North Sulawesi during 4 months from June toSeptember 2017.This research was arranged to describe the growthand yield characteristics of purple corn. Seed source used as the treatmentsis:U1 = The seed of purple corn from the fries ear, U2 = The seed of purple corn from the second ear, U3 = The Seed of purple corn from the third ear, U4 = The seed coof purple corn from the fourth ear, U5 = The seed of purple corn from the fifth ear.The results showed that the components of highplant andheight of ear position  plant height the highest is obtained from treatment U5 in contrast to the components in the diameter of the stem to be obtained from the U2 treatment being handed out. The coefficiency of variance of the growth and yield components showed low (less than 25%).  Keywords : corn characterization, purple corn
RESPON TANAMAN JAGUNG MANIS (Zea mays saccharata Sturt) TERHADAP PEMBERIAN PUPUK ORGANIK Allan Mamahit; Pemmy Tumewu; Marjam Toding
COCOS Vol. 11 No. 2 (2019): EDISI APRIL-JUNI
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v1i2.27342

Abstract

ABSTRACT This research aims to acknowledge the effect of organic fertilizing the growth and production of sweet corn which in carried out in the Village of Kauneran Satu, Sonder Sub-District during 3 months start from July – October 2018. The method of this researchment are use randomized group design with four behaviors A) None fertilizer, B) 300kg/ha of Phonska plus fertilizer, + 300 kg Urea/ha, C) 20 ton/ha organic water hyacinth fertilizer + 10 ton fertilizer from animals, D) 20 ton/ha Hydrilla Verticillata Organic fertilizer + 10 ton fertilizer from animals. The observed variable is the dried weight of the plant, the length of the cabbage, the diameter of the cabbage and the fresh weight of the sweet corn cob was measured and weighed after the sweet corn harvest. Studies have shown that treatment C 20 tons/ha organic water hyacinth fertilizer + 10 tons fertilizer from animals gives a good response to the sweet corn. Key words: Fertilizer Organic, Sweet Corn
Co-Authors Allan Mamahit Dwinita W. Utami Johannes E. X. Rogi Oksandi Darius Tumei Pemmy Tumewu Rifaine M.N. Erungan Yefta Pamandungan