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Proteolytic Activity of The Entomopathogenic Fungi (Penicillium sp. ) of Cockroaches (Periplaneta americana) Ahmad Ikhsanudin; Emantis Rosa; Christina Nugroho Ekowati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.47

Abstract

Cockroaches (Periplaneta americana) were the insect vectors of disease that caused adverse effects on human health. Control cockroch excessive use of insecticides can lead to residus in the environment and resistance cockroach. Therefore it was necessary to use an alternatives such as such as entomopathogenic fungal as biologycal agents. The entomopathogenic fungi penetrated via the integument of an cockroach to reach the hemocoel. Proteins were the molecules responsible for integument strength in cockroach, It was synthesis the proteases to degrading proteins. The study begins with the isolation of entomopathogenic fungi using the moist chamber method with cockroach as insect bait. Fungus that grow on cockroches are cultured and purifed on Potato Dextrose Agar (PDA) medium then identified. Identification was carried out through macroscopic observations including colony color and diameter and microscopic observations including conidia, conidiophores, hyphae, vesicles, fialids, and leg cells. The result of isolation and identification obtained as Penicillium sp. Proteases enzimatic activity tested on PDA with anlene 1%. The clear zone formed is measured to show the activity of proteases produced by Penicillium sp.
EVALUASI KINERJA TRANSPLANTASI Acropora Sp. MENGGUNAKAN RAK BESI PADA KONDISI HIDRODINAMIKA MUSON DI GOSONG KALANGAN, LAMPUNG Dzulfikar Yusuf Priyanatha; Vika Aulia Royani; M. Aqwam Nugraha; Flora Bintang Az Zahra; Dwi Rani Asfarini Simatupang; Aprila Mutia; Muhammad Faiz Farid; M Zona Bintang Armando; Davit Agus Priyanto; Mohammad Hafizh Afifi; Azza Dera Rahma Nayla; Priyambodo Priyambodo; Nadya Syifa Taufik; Regina Stephani Mesina Siringo-ringo; Lastiarma Manik; Fahrez Isnanta Azzaid; Jani Master; Ahmad Ikhsanudin; Naila Nur Fathiyya
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8198

Abstract

Coral reef ecosystems in Gosong Kalangan waters are experiencing degradation, thereby requiring effective rehabilitation efforts, one of which is coral transplantation of Acropora sp. using artificial media in the form of iron racks. This study aimed to analyze the survival rate and growth rate of Acropora sp. fragments transplanted using this method. The research was conducted as a field experiment employing a Completely Randomized Design (CRD) over a six-month period (July to December 2025) at a depth of approximately ±4 meters. The main parameters observed were survival rate and linear growth of coral fragments. The results showed a decline in survival rate from 100% in the first month to 56% at the end of the observation period. In contrast, the surviving fragments exhibited an increase in average length from 9.4 cm to 14.64 cm (total growth of 5.39 cm), with a mean growth rate of 0.898 cm per month. The decrease in survival rate was presumably influenced by biotic pressure in the form of predation by Drupella sp., as well as abiotic disturbances related to hydrodynamic factors and monsoonal waves affecting the stability of the transplantation racks. The iron rack method was able to support the growth rate of Acropora sp. fragments; however, its effectiveness in maintaining survival is highly dependent on the stability of local environmental conditions.