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Journal : JURNAL SAINS DAN TEKNOLOGI INDONESIA

PEMBIBITAN SECARA STEK-MINI TANAMAN MELATI [Jasminum sambac (L.) Aiton] Handayani, Titin
Jurnal Sains dan Teknologi Indonesia Vol. 8 No. 1 (2006)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.164 KB) | DOI: 10.29122/jsti.v8i1.751

Abstract

The experiment aimed at maximizing the speed growth and number of plants obtained by the mini-steck of jasmine (Jasminum sambac (L.) Aiton) cv. Emprit. Changes in benzylaminopurine (BAP), indolebutiric acid (IBA), and “rooton” concentration.were evaluated. Branchs were used as the plant material source, which consisted of axillary buds obtained after careful excision of the leaves. The following treatments were tested: solid media (soil : sand = 1 : 1) with different BAP concentrations (0.1, 3 and 5 mg/kg), IBA (0, 100, 200 and 300 ppm/kg), and “rooton” (0, 100, 200 and 300 ppm/kg) The results showed that shoots growth slowly in the media suplemented with BAP, however, IBA 200 ppm/kg media is better. Rooton with 300 ppm/kg media showed best results for rooting induction.
ANALISIS MARKA MORFOLOGI DAN MOLEKULER SIFAT KETAHANAN KEDELAI TERHADAP INTENSITAS CAHAYA RENDAH Handayani, Titin; Sastrosumarjo, Sarsidi; Sopandie, Didy; Suharsono, Suharsono; Setiawan, Asep
Jurnal Sains dan Teknologi Indonesia Vol. 8 No. 1 (2006)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.521 KB) | DOI: 10.29122/jsti.v8i1.750

Abstract

Availability of moleculer marker to identify the important agronomic character of plant is needed to accelerate selection activity of plant. Particularly for the complex quantitatively inherited traits - like e.g. shading tolerance of soybean - the use of such a technique will speed up the process to produce adapted genotypes.The objective of this research is to identify the linkage of molecular marker RAPD with character of shading tolerance. The morphological specific characters wich is corelated to shade tolerance is the number of productive branchs. The intensity 75% of artificial shading is optimal level for doing selection of soybean genotypes. The inheritance of shading tolerance of soybean was controlled by gene with full dominant or by two genes pairs with dominant and ressesive epistasis. There was no maternal effect in the inheritance to shade tolerance. Heritability value (0.45 – 0.54) indicated that the proportion variation caused by the genetic factors was moderate. Molecular analysis by using RAPD technique showed that UBC153, ROTH 480.01, and ROTH 480.03 primer have polymorphic band that can be used for inheritance study and linkage analysis. All polymorphisms segregated independently of each ather. Interval mapping with Mapmaker/QTL indicated that the location of the three QTLs on linkage group were at marker tolerance locus of Roth 480.01-8125 , Roth 480.03-1125, and UBC 153-19125 .