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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Marine Research BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Marine Research in Indonesia Indonesian Journal of Natural Pigments Teknologi Indonesia Life Science Makara Journal of Technology Annales Bogorienses

DWI SUSILANINGSIH

Research Centre for Biotechnology, Indonesian Institute of Sciences, Cibinong - Bogor, 16911, Indonesia

Author-ID : 396349

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Other

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Identification of Bioactive Compound from Microalga BTM 11 as Hepatitis C Virus RNA Helicase Inhibitor Mustopa, Apon Zaenal; Umami, Rifqiyah Nur; Putri, Prabawati Hyunita; Susilaningsih, Dwi; Farida, Hilda
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTHepatitis C virus (HCV) is the major causative agent of chronic liver disease. Recently, the inhibition of NS3 RNA helicase/ATPase activity is being explored as the specifically targeted antiviral therapy (STAT) against HCV infection. This study was aimed to elucidate potential candidates for anti-HCV therapy derived from Indonesian indigenous microalgae. The microalga designated as BTM 11 was isolated and cultured. Methanol extract of BTM 11 was screened as the opponent of purified HCV NS3 RNA helicase enzyme through colorimetric ATPase assay. Screening of chemical compound and fractionation by using gel filtration chromatography with eluent of methanol : chloroform (1:99) were conducted for identification and isolation of the bioactive compounds. The third fraction of fractionated sample showed a relatively strong ATPase inhibitory effect (81.23 Â± 2.25 %) compared to the negative control. Further analysis of third fraction using thin layer chromatography (TLC) with eluent of chloroform : methanol (9:2) gave two spots with the Rf value of 0.8 and 0.37, respectively. In addition, high performance liquid chromatography (HPLC) analysis showed absorption peak with the highest abundance at the retention time of 12.483 and 16.617 minutes which absorbed at 266 and 230 nm wavelenght, respectively. According to those analyses, this study suggests that bioactive compounds derived from BTM 11 were classified as the groups of flavonoids and feasible as potential candidates for anti-HCV therapy through the inhibitory effect of NS3 RNA helicase/ATPase activity.Â Keywords: Hepatitis C Virus, NS3 RNA helicase, ATPase, Microalga, FlavonoidsÂ

Isolation and Screening of Surfactant-producing Bacteria from Indonesian Marine Environments and Its Application on Bioremediation Susilaningsih, Dwi; Okazaki, Fumiyoshi; Yopi, Yopi; Widyastuti, Yantyati; Harayama, Shigeaki
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Isolation and screening have been undertaken on oil-degrading microbes from Indonesian marine environments. During screening process it has been found many bacterial isolates capable of degrading crude oil. Hence, study has been focused on the biodiversity of biosurfactant-producing bacterial species in Indonesian marine environment and its function for remedial the pollutant in marine and soil areas. A total of 103 out of 463 isolates showed positive surfactant-degrading properties. By means of partial 16S rRNA gene analyses, it has been found that the majority of taxa are related to Alcanivorax, Pseudomonas, Bacillus, Bortetela, Brucella, Acenitobacter, Staphia, Lysobacter, and Talasosophira. Biosurfactant properties assay showed that they were capable of lowering the surface- and interfacial water tension from 74 mN/m to 40-65 mN/m and from 24 mN/m to 6-10 mN/m, respectively. In addition, most of the surfactants were capable of emulsifying hydrocarbon (crude oil) of 0.01 to 0.15 units, comparable to 0.08 units of synthetic surfactant (20% Tween). Further observation showed that the majority of the surfactants were able to degrade a long chain of alkane, but not branched alkane, with a recovering rate of 20-80%. The application of the surfactant towards oil polluted model beach was done in laboratory scale and showing the surfactant obtained from microbial broth cultures capable for recovering the oil pollutant significantly, compared to the control (without addition microbial broth). Â

Molecular Identification of Microalgae BTM 11 and its Lectin Isolation, Characterization, and Inhibition Activity Mustopa, Apon Zaenal; Isworo, Rhestu; Nurilmala, Mala; Susilaningsih, Dwi
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v20i2.251

BTM 11 is unknown species of microalgae, but has active compounds that can inhibit viruses. One of proteins produced by microalgae is a lectin. Lectin is a carbohydrate-binding protein found in various microalgae that show antiviral and antibacterial activity. The purpose of this study was to perform identification of the species of microalgae BTM 11, isolation, characterization, and assay of lectin inhibitory activity. Microalgae BTM 11 was identified to have homology with Cyanobacterium 99% and Geitlerinema sp 98%. Lectin of microalgae BTM 11 was isolated by ammonium sulfate precipitation of 75% with a molecular weight of 17 kDa. Lectin protein activity of microalgae BTM 11 was able to inhibit the enzyme activity of RNA helicase hepatitis C by 57.90% and 27.55%. In addition, the protein was able to suppress the activity of Staphylococcus aureus ATCC 6538, E. coli EPEC K.1.1. and Salmonella typhii ATCC 25241. Activitiy of lectin was stable at 30 Â°C and was unaffected by the action of the enzyme. These results indicate that lectin of microalgae BTM 11 could be a alternative to antiviral and antibacterial proteins.

BIODIVERSITY OF HYDROCARBON-PRODUCING MICROALGAE FROM OIL CONTAMINATED IN COASTAL ZONE OF BATAM ISLAND Susilaningsih, Dwi; Siburian, Marlinang Diarta; Murniasih, Tutik
Marine Research in Indonesia Vol 33, No 2 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Addressing to the environmental and energy crises in Indonesia, the new alternative of green-economic-energy sources is required. In this study marine microalgae were isolated from oil-polluted coastal zone in Batam Island for alternative energy sources. The research was carried out by collecting the sea water samples from several peninsulas of Batam Island. The abundance of microalgal diversity was observed. The isolation was done by capillary micropipette technique. The method of specific screening for hydrocarbon-producing microalgae was done using selected media, CO2 rate and light intensity period treatments. The results showed that the Cyanobacteria and diatom occupied the Batams coastal waters. From the isolated samples, 11 strains positively deposit the hydrocarbon in their cells. Further study for determining the isolates and their properties is still in progress.

HYDROGEN PRODUCTION USING Rhodobium Marinum IN MILK LIQUID WASTE Anam, Khairul; Susilaningsih, Dwi
Teknologi Indonesia Vol 38, No 1 (2015)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v38i1.118

This study was conducted to investigate the effect of dilution towards milk liquid waste in biologically producing hydrogen gas by photosynthetic bacteria, Rhodobium marinum. The methods included pretreatments of the milk liquid waste by sterilization, filtration, neutralization, and centrifugation, before and after dilution. From several experiments, 100% of milk liquid waste substrate generated the highest hydrogen gas production up to 93.45 16.15 ml, but the highest efficiency was obtained at level 12.5% of milk liquid waste substrate which valued 0.33 mol hydrogen/ mol glucose consumed. Kinetics measurements for hydrogen production process in milk liquid waste which was added with nutrients obtained yield of biomass formed per substrate consumed (Yx/s) i.e. 0.192 g/g, yield of product per substrate (Yp/s) i.e. 0.009 g/g, and yield of product produced per biomass (Yp/x) i.e. 0.043 g/g. For kinetics measurements on the milk liquid waste medium without nutrients addition, obtained Yx/s was 0.261 g / g, Yp/s was 0.019 g/g and Yp/x was 0.070 g/g

CULTIVATION OF FILAMENTOUS CYANOBACTERIA FOR VALUABLE BIOPRODUCT USING SAGO SOLID WASTE AS SUBSTRATES Susanti, Hani; Lestari, Sari; Rahman, Delicia Y.; Susilaningsih, Dwi
Teknologi Indonesia Vol 37, No 1 (2014)
Publisher : LIPI Press

Sago solid waste as by product result of sago palm processing potentially have great value for some bio processing such as source of carbon on microbes substrate. Utilization of sago solid waste as substrate for filamentous cyanobacteria cultivation is a rareresearch activity in Indonesia as one of the three leading world companies producer of sago starch. Therefore, we focused on sago waste treatment using cyanobacteria agent in order to rid the waste and gain the value of nutrition rich starch from that waste. Several concentrations of growth media contain sago solid waste were tested into cyanobacteria culture and solid waste hydrolysis as pretreatment more conducted in the research. Result shows that addition of sago solid waste (50, 500 and 5000 ppm) into media can increase the algae biomass production which is rich of carbohydrate and protein.The proximate value of biomasses with sago solid waste treatment was exhibited significantly higher than control. During cultivation exposure time, the solid waste is gradually consumed by the algae. The study implies that sago solid waste can be used as substrate for the biomass production of filamentous cyanobacteria which is known as source of active compounds, potential resource for valuable bioproduct.

ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Susilaningsih, Dwi; Umoro, Asahedi; Ochieng, Fredrick Onyango; Widyaningrum, Dian Noverita; Susanti, Hani; Susilo, Hadi; Swastika, I Nengah; Widyastuti, Utut
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ?Carita? Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids.

EFIKASI LIMBAH SAGU SEBAGAI SUBSTRAT KAYA NUTRISI UNTUK MIKROALGA ISOLAT LIPI11-2-AL002 Susilaningsih, Dwi; Lestari, Sari; Kusnadi, Kusnadi; Hidayat, Topik; Susanti, Hani
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.674

Microalgae are photosynthetic microorganisms that have potential to produce some useful chemical substances such as carbohydrates, proteins, and lipids. Microalgae are also known exhibited ability as a bioremediation agent. This report is emphasized on analyzing the growth rate and nutritional content of microalgae including carbohydrate, protein and lipid from selected isolates LIPI11-2-AL002 that treated with sago waste. Microalgae were cultivated into hydrolysed sago ?ampas? at the concentration of 0,50, 500,5000 ppm, and medium AF-6 (as control culture) respectively.Therefore selected microalgae isolate was cultivated in series of cultivation volume start from 100 mL until 5 Liter media gradually. Observed parameters were covered cell viability (growth) and proximate content of biomass including carbohydrate,lipid and protein content. The results showed that the carbohydrate and protein content in the algal biomass was increase along the addition of series sago ?-ampas? concentration.The highest concentration of addition the sago ampas is 5000 ppm which is limit for the algal survival. In the highest treatment of sago waste the alga l proximate contents were 261.09 ppm of carbohydrate, 5.12 ppm of protein and 3.61% per of lipid dry weight respectively. In addition, the toxicity effect of fermentation product was not appeared in a toxicity test using gold fish komet(Carassius auratus).

Morphology and molecular characterization of newly isolated microalgae strain Chlorella volutis LIPI13-WKT066 from Wakatobi Islands and its potential use Rahman, Delicia Yunita; Praharyawan, Swastika; Raharjo, Sapto; Fadiyah, Farizul; Susilaningsih, Dwi
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2019.v23.n1.13-19

Morphology and molecular characterization of microalgae isolated from Wakatobi Marine National Park was conducted. An understanding of the characteristics of morphology, molecular, as well as metabolites profile of the microalgae species is potentially useful for its future application. The primary aim of this study was to isolate, identify and characterize the microalgae strain isolated from Wakatobi Marine National Park labeled as LIPI13-WKT066 with the emphasis on the evaluation of amino acid content as a basis for its health application. Morphological observation under the microscope and molecular identification suggested that the microalgae strain of LIPI13-WKT066 belong to the strain under species of Chlorella volutis. Metabolite characterization of the microalgae strain showed that the content of protein (11.9%), lipid (12.4%) and carbohydrate (4.7%) was in the regular range. Further analysis of its amino acid content revealed the potency of the microalgae strain to be used as antihypertensive agent.

BIODIVERSITY OF HYDROCARBON-PRODUCING MICROALGAE FROM OIL CONTAMINATED IN COASTAL ZONE OF BATAM ISLAND Susilaningsih, Dwi; Siburian, Marlinang Diarta; Murniasih, Tutik
Marine Research in Indonesia Vol 33 No 2 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Addressing to the environmental and energy crises in Indonesia, the new alternative of green-economic-energy sources is required. In this study marine microalgae were isolated from oil-polluted coastal zone in Batam Island for alternative energy sources. The research was carried out by collecting the sea water samples from several peninsulas of Batam Island. The abundance of microalgal diversity was observed. The isolation was done by capillary micropipette technique. The method of specific screening for hydrocarbon-producing microalgae was done using selected media, CO2 rate and light intensity period treatments. The results showed that the Cyanobacteria and diatom occupied the Batam's coastal waters. From the isolated samples, 11 strains positively deposit the hydrocarbon in their cells. Further study for determining the isolates and their properties is still in progress.

Co-Authors A.A. Ketut Agung Cahyawan W Anam, M. Khairul Anhari, Saeful Antonius Budi Susanto Apon Zaenal Mustopa Delicia Yunita Rahma Fadiyah, Farizul Farida, Hilda Farida, Hilda Hadi Susilo, Hadi Hani Susanti, Hani Harayama, Shigeaki Harayama, Shigeaki Harwati, Theresia Umi Ibnul Mubarok Isworo, Rhestu Isworo, Rhestu Leenawaty Limantara Mala Nurilmala Ochieng, Fredrick Onyango Okazaki, Fumiyoshi Okazaki, Fumiyoshi Puspita Sari Harahap Putri, Prabawati Hyunita Putri, Prabawati Hyunita Rahman, Delicia Y. Rahman, Delicia Yunita Rifana Sobari Rifqiyah Nur Umami, Rifqiyah Nur Rosita Dwi Chandra, Rosita Dwi Salim, Katarina Purnomo Sapto Raharjo Siburian, Marlinang Diarta Siburian, Marlinang Diarta Siti Harnina Bintari Swastika Praharyawan, Swastika Swastika, I Nengah Tatas H.P. Brotosudarmo Theresia Harwati, Theresia Topik Hidayat Tutik Murniasih Umoro, Asahedi UTUT WIDYASTUTI Widyaningrum, Dian Noverita Yantyati Widyastuti YOPI YOPI Yundari, Yundari

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