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All Journal Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi
Risa Nursanty
Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Education

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The Effect of Methanol Extract of Leaves of Hibiscus Rosa-Sinensis L. against Methicillin-Resistant Staphylococcus Aureus (MRSA) Mona Fathia; Risa Nursanty; Nurdin Saidi
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 7, No 1 (2015): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

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Abstract

This study was aimed to determine the effect of the methanol extract against bacteria Methicillin Resistant Staphylococcus aureus (MRSA) and to measure its inhibition capacity. Phytochemical test and extraction were conducted at the Research Laboratory of Chemistry Department, Faculty of Mathematics and Natural Sciences (MIPA). Antibacterial test was done at the Laboratory of Microbiology Department of Biological Science, University of  Syiah Kuala. The samples used leaves of H. rosa-sinensis collected from Gampong Sektor Timur, Darussalam.This study used completely randomized design (CRD) with five treatements and three replications namely; negative control using the solvent, extract test with  concentrations 0.2 g / mL, 0.3 g / mL, 0.4 g / mL, and  positive control using 30 µg linezolid. Phytochemical test showed that the fresh samples contain alkaloids, terpenoids, steroids, and flavonoids, while the methanol extract contains alkaloids and flavonoids. Antibacterial test showed that the methanol extract have inhibited MRSA bacterial growth, with the average of inhibition zone was 15.90 mm at the concentration of 0.4 g / mL.
Potensi Antibakteri Beberapa Tumbuhan Obat Tradisional Risa Nursanty; Zumaidar Zumaidar
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 2, No 3 (2010): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

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The research of antibacterial ability from Anacardium occidentale (Anacardiaceae), Lawsonia inermis (Lythraceae), Averrhoa bilimbi, Averrhoa carambola (Oxalidaceae), Capsicum frutescens and Solanum torvum (Solanaceae) to Escherichia coli ATCC 25922 and Staphylococcus  aureus ATCC 29213 was done from Mei until November 2010 at Microbiology laboratory, Natural Science, Syiah Kuala University. Leave and barks extracts of plant was soluted using n-Hexane solution. Antibacterial activities was tested by using diffusion method with each concentrations 10%, 20%, and 30%. The result showed that plants leaves and barks had ability to inhibit the growth of Staphylococcus  aureus ATCC 29213. The largest of inhibition zone was formed by L. inermis leaves extract in 6 mm. Meanwhile C. frutescens barks extract was showed inhibition zone in 5 mm.
BIOPROSPECTING SOIL ACTINOMYCETES: ISOLATION AND ANTIBACTERIAL ASSAY Suhartono Suhartono; Risa Nursanty
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 4, No 1 (2012): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

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Abstract

Searching for novel bioactive compounds of microorganisms is increasing. Actinomycetes are a group of bacteria that have potential as a major producer of bioactive compounds. This study aimed to isolate and evaluate the isolates of actinomycetes collected from soil. This research was carried out in three stages, namely the field sampling, isolation of actinomycetes, screening the potential as a producer of bioactive compounds (antimicrobials). A total of 11 isolates of actinomycetes have been isolated in this study. The isolates varied in morphological characteristics. The antibacterial evaluation showed that nine isolates (81.8%) that were able to inhibit either the test organism. Three isolates of ACT-04, ACT-05, ACT-06 and ACT-11 were capable of inhibiting both bacterial targets.
Co-Authors Fathia, Mona Nurdin Saidi Suhartono Suhartono Zumaidar Zumaidar