Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi
Vol 7, No 1 (2015): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi

The Effect of Methanol Extract of Leaves of Hibiscus Rosa-Sinensis L. against Methicillin-Resistant Staphylococcus Aureus (MRSA)

Mona Fathia (Universitas Syiah Kuala)
Risa Nursanty (Universitas Syiah Kuala)
Nurdin Saidi (Universitas Syiah Kuala)



Article Info

Publish Date
03 Dec 2016

Abstract

This study was aimed to determine the effect of the methanol extract against bacteria Methicillin Resistant Staphylococcus aureus (MRSA) and to measure its inhibition capacity. Phytochemical test and extraction were conducted at the Research Laboratory of Chemistry Department, Faculty of Mathematics and Natural Sciences (MIPA). Antibacterial test was done at the Laboratory of Microbiology Department of Biological Science, University of  Syiah Kuala. The samples used leaves of H. rosa-sinensis collected from Gampong Sektor Timur, Darussalam.This study used completely randomized design (CRD) with five treatements and three replications namely; negative control using the solvent, extract test with  concentrations 0.2 g / mL, 0.3 g / mL, 0.4 g / mL, and  positive control using 30 µg linezolid. Phytochemical test showed that the fresh samples contain alkaloids, terpenoids, steroids, and flavonoids, while the methanol extract contains alkaloids and flavonoids. Antibacterial test showed that the methanol extract have inhibited MRSA bacterial growth, with the average of inhibition zone was 15.90 mm at the concentration of 0.4 g / mL.

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Journal Info

Abbrev

JBE

Publisher

Subject

Agriculture, Biological Sciences & Forestry Education

Description

Jurnal Biologi Edukasi (J.BioEd) contains the results of biology and biology education research from many educational institutions in Indonesia. It published twice a year in June and ...