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ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT OF WHITE AND RED FLESH FROM GUAVA LEAF ( Psidium guajava. L) AGAINTS Staphylococcus aureus AND Escherichia coli Hilda Maysarah; Rika Apriani; Misrahanum Misrahanum
Jurnal Natural Volume 16, Number 1, March 2016
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.007 KB) | DOI: 10.24815/jn.v16i1.4818

Abstract

An antibacterial activity test of ethanol extract of white and red flesh from guava leaf (Psidium guajava. L) against S.aureus and E.coli; using agar diffusion method was carried out in order to produce the extract. The extract was collected using maceration method. The concentration of extract was 7,8125; 6,1035; 5,00; 4,8828; 4,3944; and 3,90625 mg/mL. The results showed that both of extracts had antibacterial activities. Ethanol extract of white flesh of fruit guava leaf had (Minimum Inhibitory Concentration) MIC value at 5.000 mg/mL against S.aureus and 4.8828 mg/mL against E.coli. Whereas ethanol extract of red flesh of fruit guava leaf had MIC value at 4.3944 mg/mL against S.aureus and E.coli.  MIC value of ethanol extract of white flesh of fruit guava leaf is equal with MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli. The MIC value of red flesh of fruit guava leaf is equal to the MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli.
ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT OF WHITE AND RED FLESH FROM GUAVA LEAF ( Psidium guajava. L) AGAINTS Staphylococcus aureus AND Escherichia coli Hilda Maysarah; Rika Apriani; Misrahanum Misrahanum
Jurnal Natural Volume 16, Number 1, March 2016
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v16i1.4818

Abstract

An antibacterial activity test of ethanol extract of white and red flesh from guava leaf (Psidium guajava. L) against S.aureus and E.coli; using agar diffusion method was carried out in order to produce the extract. The extract was collected using maceration method. The concentration of extract was 7,8125; 6,1035; 5,00; 4,8828; 4,3944; and 3,90625 mg/mL. The results showed that both of extracts had antibacterial activities. Ethanol extract of white flesh of fruit guava leaf had (Minimum Inhibitory Concentration) MIC value at 5.000 mg/mL against S.aureus and 4.8828 mg/mL against E.coli. Whereas ethanol extract of red flesh of fruit guava leaf had MIC value at 4.3944 mg/mL against S.aureus and E.coli.  MIC value of ethanol extract of white flesh of fruit guava leaf is equal with MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli. The MIC value of red flesh of fruit guava leaf is equal to the MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli.
Antibacterial Activity of Citronella Essential Oil from Cymbopogon nardus (L.) Rendle) Against Methicillin-Resistant Staphylococcus aureus Sari, Irma; Misrahanum, Misrahanum; Faradilla, Meutia; Ayuningsih, Cut Mutia; Hilda Maysarah
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 5 No. 1 (2022): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/idjpcr.v5i1.8302

Abstract

Irrational antibiotic use in the treatment of infectious diseases can result in the emergence of antibiotic-resistant bacterial strains, such as Methicillin Resistant Staphylococcus aureus (MRSA). The purpose of this study is to determine the physical properties of citronella essential oil (Cymbopogon nardus (L.) Rendle) and to assess its antibacterial activity against MRSA. Citronella essential oil was extracted using a water-steam distillation method, yielding 0.5 percent. Citronella essential oil has a specific gravity of 0.904, a refractive index of 1.470, and is slightly soluble in 80 percent ethanol. All of these parameters were in accordance with the Indonesian Standard Requirement (SNI) for Citronella essential oil. The disc diffusion assay was used to test the antibacterial activity of this Citronella essential oil. The result indicated that Citronella essential oil has an activity to inhibit MRSA invitro.