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All Journal Jurnal Natur Indonesia Indonesia Chimica Acta Jurnal Progres Kimia Sains
Tjodi Harlim
Faculty of Mathematic and Natural Science, Chemistry Department, Hasanuddin University, Makasar.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

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PRODUKSI SENYAWA BAKTERIOSIN SECARA FERMENTASI MENGGUNAKAN ISOLAT BAL Enterococcus faecium DU55 DARI DANGKE Razak, Abd. Rahman; patong, Abd. Rauf; Harlim, Tjodi; Djide, M. Natsir; Haslia, Haslia; Mahdalia, Mahdalia
Indonesia Chimica Acta Volume 2 No 2 - December 2009
Publisher : Indonesia Chimica Acta

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Dangke is one of traditional food from Enrekang Province Sulawesi Selatan which is made from buffalo milk and enzimatically processed using papain from papaya’s gland secretion. Research on this local product as source of lactic acid bacteria (LAB) has been done. Counted 30 LAB was successfully isolated and 3 of them were potentially to be able to yield antimicrobial compound. Enterococcus faecium DU55 is one of LAB isolate available to be applied for producing bacteriocin compound through fermentation. Optimum condition of fermentation was specified by determining the highest antimicrobial activity generated by filtrate of fermentation result. Antimicrobial activity examination was carried out through diffusion agar method by measuring inhibition zone growth of pathogen bacterium Salmonella typhimurium FNCC 0050. Research results indicate that maximum bacteriocin compound production by BAL isolate E. faecium DU55 was obtained at condition of optimum fermentation at 30 °C during 42 hour using M1 medium with the same composition to medium MRS (Man Rogosa and Sharpe).Keyword: dangke, LAB isolate, bacteriocin, antimicrobial activity
Skrining Bioaktivitas Beberapa Bagian Jaringan Tumbuhan Paliasa(Melochia umbellata (Hout) Stapf var. Degrabrata K) Erwin, Erwin; Noor, Alfian; Soekamto, Nunuk Hariani; Harlim, Tjodi
Indonesia Chimica Acta Volume 2 No 1 - June 2009
Publisher : Indonesia Chimica Acta

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Paliasa is one of tropical plants that used as an etnobotanical traditional drug by the people of South Sulawesi. Paliasa leaves is believed and used as a drug for hypertension, diabetes mellitus, cholesterol, and hepatitis diseases. Preliminary study was done by Brine shrimp lethality test with Artemia salina to a tissue part of one of type paliasa, Melocia umbellata (Houtt.) Stapf. var. degrabrata K. The result show that the wood of this plant is the most active with LC50 value of 1,80 ppm. Keywords : Paliasa, traditional drug, M. umbellata, bioactivity.
Penentuan Konstanta a Dan K Dalam Persamaan Mark-Houwink- Sakurada (MHS) Untuk Menentukan Massa Molekul Poli (Asam Laktat) Diol ., Saefuddin; Noor, Alfian; Harlim, Tjodi; Taba, Paulina; Zaini, Ahmad; ., Zarlina
Jurnal Progres Kimia Sains Vol 1, No 1 (2011): JPKS Open Acces
Publisher : Universitas Halu Oleo

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Poli(asam laktat) diol (PLA-OH) telah disintesis melalui reaksi polimerisasi kondensasi asam laktat dan 1,4-butanadiol. Berat molekul rata-rata (Mn, Mw, dan Mz) PLA-OH ditentukan dengan analisis menggunakan Gel Permeation Chromatography (GPC). Viskositas intrinsiknya diukur pada konsentrasi 0,2 g/dL dan temperatur 298 K menggunakan pelarut kloroform. Melalui metode numerik berhasil ditentukan nilai a dan K dalam persamaan Mark- Houwink-Sakurada untuk PLA-OH, yaitu [η] = 3,532 x 10-4Mv 0,628 = 3,532 x 10-4qMHS 0,628 = 3,415 x 10-4Mw 0,628
Aktivitas Antibakteri Senyawa Turunan Oleanan dari Kayu Akar Pterospermum Subpeltatum Salempa, Pince; Noor, Alfian; Hariani, Nunuk; Harlim, Tjodi
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.458 KB) | DOI: 10.31258/jnat.13.1.59-62

Abstract

A triterpenoid derivative compound, oleane-12-en-2,3,23-triol-28-oat, from chloroform fraction of root wood ofPterospermum subpeltatum C. B. Rob (Sterculiaceae) was identified using IR, 1H dan 13C NMR data. The compoundhas shown toxicity against Artemia salina, LC50 of 46.06 μg/mL, and antibacterial potential upon Shigella boydii andStaphylococcus aureus with inhibition zones of 8.3 and 9.8 mm respectively.
Co-Authors Abd. Rahman Razak Abd. Rauf Patong Ahmad Zaini Alfian Noor Asnah Marzuki Erwin Erwin Haslia Haslia, Haslia M. Natsir Djide Mahdalia Mahdalia, Mahdalia Nunuk Hariani Nunuk Hariani Soekamto Nunuk Soekamto Paulina Taba Pince Salempa Saefuddin ., Saefuddin Zarlina ., Zarlina