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All Journal Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) UNEJ e-Proceeding International Journal of Applied Biology Indonesian Journal of Chemistry JCES (Journal of Character Education Society) Jurnal Widya Medika Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)
Lanny Hartanti
Widya Mandala Catholic University Surabaya
Agriculture, Biological Sciences & Forestry Automotive Engineering Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Environmental Science Medicine & Pharmacology

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Screening and Isolation of Cellulolytic Bacteria From Bagasse and Characterization of The Cellullase Produced Hartanti, Lanny; Susanto, Fandy; Utami, Caesilia Putri; Sukarti, Emi; Setiawan, Henry Kurnia; Ervina, Martha
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The research of screening and isolation of cellulase-producing bacteria from bagasse had been done. The objectives of the research were to obtain cellulolytic bacteria isolate, to determine the genus of the isolates and also to characterize the crude enzymes produced. One isolate with the highest activity was chosen to be purified further bystreak plate and pour plate methods in carboxyl methyl cellulose(CMC)  agar media. The genus of the pure isolated bacteria was further characterized by macroscopic and microscopic properties, and biochemical assays using conventional methods and MicrobactTM Kit: gram-negative identification system (Oxoid). The result showed that the isolated cellulolytic bacteria had a close character similarity to Bacillus pumilus. The isolate was grown on MHB + CMC media with 2 hours of adaptation phase followed by logarithmic phase until 21 hours of incubation. Cellulase was started to be produced after 4 hours of fermentation and reached the highest activity at 21 hours of fermentation. The cellulase produced had the pH optimum at pH 5 and a temperature optimum at 60 °C, with optimum activity ranging from 0.0231 to 0.0264 U /ml. One unit of cellulase activity is defined as the amount of enzyme that will catalyze the production of 1 mg of glucose per minute at 37°C and pH 7.0. Keywords:  bagasse, bacteria isolation, cellulolytic, biochemical characterization, enzyme characterization
Studi pendahuluan: Korelasi Aktivitas Antikolesterol dengan Aktivitas Antioksidan Ekstrak Etanol Daun Salam (Syzygium polyanthum) Wijaya, Sumi; Yonas, Stefani Maureen Kasih; Hartanti, Lanny; Setiawan, Henry K.; Soegianto, Lisa
Jurnal Farmasi Sains dan Terapan Vol 5, No 2 (2018)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2982.965 KB) | DOI: 10.33508/jfst.v5i2.2143

Abstract

Bay leaf leaves (Syzygium polyanthum) are known to have anti-cholesterol activity, with the mechanism inhibited HMG-CoA Reductase enzyme. The purpose of this study was to determine the correlation between anticholesterol activity of bay leaf ethanol extract obtained by percolation method and its antioxidant activity. The parameter used to measure anticolesterol activity is the IC50 value. Antioxidant activity was evaluated by DPPH and Frap methods. The results showed that the ethanolic extract of bay leaves had an IC50 value of 49.50 ± 0.70 ppm. Ethanol extract of bay leaf showed antioxidant activity with IC50 value of 888.0835 ppm and equality of values with 1 mM FeSO4 of 295 ppm. Based on the results of the analysis, the anticholesterol activity of Bay leaf ethanol extract in inhibiting the HMG-CoA Reductase enzyme did not have a linear correlation with its antioxidant activity.
Uji Aktivitas Inhibitor Xanthin Oksidase dari Fraksi Ekstrak Etanol Herba Peperomia pellucida Prihastuti, Angelina Ajeng; Wijaya, Sumi; Hartanti, Lanny
Jurnal Farmasi Sains dan Terapan Vol 4, No 1 (2017)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3407.524 KB) | DOI: 10.33508/jfst.v4i1.2174

Abstract

Peperomia pellucida (L.) Kunth known as Suruhan is a potential medicinal plants, used traditionally to treat gout. Suruhan herb (Peperomia pellucida (L.) Kunth) had been studied in vivo, and found to be able to lowering uric acid levels in the blood. Ethanol extract of Peperomia pellucida (L.) Kunth herb also had been studied in vitro, and found to be potential to inhibit xanthine oxidase. The purpose of this study is to know the potential of fraction from ethanol extract of Peperomia pellucida (L.) Kunth. herb as xanthine oxidase inhibitor compared to allopurinol and its ethanolic extract. Ethanol extract of Peperomia pellucida (L.) Kunth herb was obtained by percolation method using ethanol 96%. The fractionation is done by column chromatography method using silica as stationary phase and n-hexane, n-hexane-ethyl acetate, ethyl acetate, ethyl acetate-ethanol, and ethanol as mobile phases. The fraction which contained flavonoid compounds was tested its xanthine oxidase inhibition potency using UV spectrophotometer at λ 290 nm. The absorbance was observed every 10 seconds for 10 minutes for extract and fraction with the concentration of 0.25 ppm - 5 ppm, while allopurinol was determined with the concentration of 0.2 ppm - 3.2 ppm. The result showed that ethyl acetate-ethanol fraction potentially inhibited xanthine oxidase with IC50 value of 5.00 ± 0.06 ppm, while ethanolic extract of Peperomia pellucida (L.) Kunth herb and allopurinol have IC50 value 0.33± 0.07 ppm and 0.84 ± 0.02 ppm respectively. Thus it can be concluded that ethyl acetateethanol fraction had potential as xanthine oxidase inhibitor, but the potential is lower than the ethanolic extract of Peperomia pellucida (L.) Kunth herb.
Screening and Isolation of Cellulolytic Bacteria From Bagasse and Characterization of The Cellullase Produced Lanny Hartanti; Fandy Susanto; Caesilia Putri Utami; Emi Sukarti; Henry Kurnia Setiawan; Martha Ervina
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The research of screening and isolation of cellulase-producing bacteria from bagasse had been done. The objectives of the research were to obtain cellulolytic bacteria isolate, to determine the genus of the isolates and also to characterize the crude enzymes produced. One isolate with the highest activity was chosen to be purified further bystreak plate and pour plate methods in carboxyl methyl cellulose(CMC)  agar media. The genus of the pure isolated bacteria was further characterized by macroscopic and microscopic properties, and biochemical assays using conventional methods and MicrobactTM Kit: gram-negative identification system (Oxoid). The result showed that the isolated cellulolytic bacteria had a close character similarity to Bacillus pumilus. The isolate was grown on MHB + CMC media with 2 hours of adaptation phase followed by logarithmic phase until 21 hours of incubation. Cellulase was started to be produced after 4 hours of fermentation and reached the highest activity at 21 hours of fermentation. The cellulase produced had the pH optimum at pH 5 and a temperature optimum at 60 °C, with optimum activity ranging from 0.0231 to 0.0264 U /ml. One unit of cellulase activity is defined as the amount of enzyme that will catalyze the production of 1 mg of glucose per minute at 37°C and pH 7.0. Keywords:  bagasse, bacteria isolation, cellulolytic, biochemical characterization, enzyme characterization
INHIBITORY POTENTIAL OF SOME SYNTHETIC CINNAMIC ACID DERIVATIVES TOWARDS TYROSINASE ENZYME Lanny Hartanti; Henry Kurnia Setiawan
Indonesian Journal of Chemistry Vol 9, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1511.645 KB) | DOI: 10.22146/ijc.21579

Abstract

Cinnamic acid is one of known tyrosinase inhibitors. This study investigated the inhibition of tyrosinase activity of some cinnamic acid derivatives, i.e. 4-buthoxy-cinnamic acid, 4-n-butylcinnamic acid and 4-phenylcinnamic acid. Each inhibitor used in this research had the same type of inhibition towards enzymatic activity, i.e. mixed type inhibition of competitive and non competitive type. The potential sequence of tyrosinase inhibition based on the ratio of its IC50 compared to cinnamic acid, from the lowest to the highest were 4-buthoxycinnamic acid, 4-phenylcinnamic acid and 4-n-butylcinnamic acid. Based on the obtained results of this research, it was disclosed that n-butyl substituent on para position did not increase the inhibition effect of cinnamic acid towards tyrosinase enzymatic reaction. But in the other hand, buthoxy and phenyl substituent on para position could increase the inhibition effect of cinnamic acid towards tyrosinase enzymatic reaction because buthoxy substituent increased the similarity of its structure with the substrate of enzymatic reaction while phenyl susbtituent blocked the substrate-enzyme reaction.
SYNTHESIS OF SOME CINNAMIC ACID DERIVATIVES: EFFECT OF GROUPS ATTACHED ON AROMATIC RING TO THE REACTIVITY OF BENZALDEHYDE Marcellino Rudyanto; Lanny Hartanti
Indonesian Journal of Chemistry Vol 8, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (78.964 KB) | DOI: 10.22146/ijc.21626

Abstract

Synthesis of cinnamic acid and its six derivatives has been done by employing Knoevenagel reaction. Benzaldehyde, 4-butylbenzaldehyde, 4-t-butylbenzaldehyde, 4-butoxybenzaldehyde, 4-phenylbenzaldehyde, 5-bromo-2,4-dimethoxybenzaldehyde, and 5-bromo-2,3-dimethoxybenzaldehyde were reacted with malonic acid in pyridine – piperidine to give cinnamic acid (85,3%), 4-butylcinnamic acid (69,3%), 4-t-butylcinnamic acid (77,7%), 4-butoxycinnamic acid (64,5%), 4-phenylcinnamic acid (65,5%), 5-bromo-2,4-dimethoxycinnamic acid (53,2%) and 5-bromo-2,4-dimethoxycinnamic acid (57,2%), respectively. It was disclosed that 4-alkyl, 4-alkoxy, 4-aryl, dan 2-alkoxy groups decrease the reactivity of carbonyl carbon of benzaldehyde.
ONE-STEP CONVERSION OF EUGENOL TO METHYL ISOEUGENOL USING MICROWAVE IRRADIATION IN SOLVENT-FREE CONDITIONS Marcellino Rudyanto; Lanny Hartanti
Indonesian Journal of Chemistry Vol 6, No 3 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.996 KB) | DOI: 10.22146/ijc.21734

Abstract

A research on conversion of eugenol to methyl isoeugenol via one-step reaction with microwave irradiation has been carried out. Mixtures containing eugenol, sodium or potassium carbonate as solid support, with or without sodium or potassium hydroxide as base, with or without tetrabutylammonium bromide as phase transfer catalyst, with dimethyl sulfate as the methylating agent were irradiated in a domestic microwave oven for 20 - 50 seconds. It was revealed that one-step methylation and isomerization required combinations of sodium or potassium hydroxide base and tetrabutylammonium bromide. Without combination of base and TBAB only one product, i.e. methyl eugenol, was formed.
PHAGO PHARMACOLOGY ON THE GO. GAMIFICATION APPROACH IN CASE LEARNING PROCESS Evelyn Ongkodjojo; F.V Lanny Hartanti; Bernadette Dian Novita; Sianty Dewi; Dyana Sarvasti; Diga Albrian Setiadi; Fransiskus Reynaldy; Ivan Priyantono; Shavna Arfi Rasyid; Felix Kurniawan Adithia; Iwan Satria Djuanda
JURNAL WIDYA MEDIKA Vol. 8 No. 1 (2022): Supplement Juni
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v8i1.4024

Abstract

Learning process among medical students have developed along with technological advances so that it can be useful for users. One of the breakthroughs in the world of medical education is the development of serious games using multimedia technology that are relatively small in size so that they can be accessed using computers or devices named Pharmacology on the Go (PHAGO). The implementation stage was done by testing prototype game on 88 users October 20, 2021. Evaluation was done by using a feedback questionnaire for PHAGO users, shows 79 (89.8%) users who agree and strongly agree that the PHAGO application helps users understand the use of Pharmacology for clinical application. The results of the Wilcoxon test in evaluating the increase in students' knowledge with Pre Test and Post Test showed that there was a significant difference (p = 0.000). The existence of serious game stimulates an active learning process to create a learning environment that encourages the development of interpersonal, communication and problem solving skills. PHAGO is expected to be a forum that supports students to be able to independently study pharmacology in a fun and easy-to-understand way.
Studi pendahuluan: Korelasi Aktivitas Antikolesterol dengan Aktivitas Antioksidan Ekstrak Etanol Daun Salam (Syzygium polyanthum) Wijaya, Sumi; Yonas, Stefani Maureen Kasih; Hartanti, Lanny; Setiawan, Henry K.; Soegianto, Lisa
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 5 No. 2 (2018)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v5i2.2143

Abstract

Bay leaf leaves (Syzygium polyanthum) are known to have anti-cholesterol activity, with the mechanism inhibited HMG-CoA Reductase enzyme. The purpose of this study was to determine the correlation between anticholesterol activity of bay leaf ethanol extract obtained by percolation method and its antioxidant activity. The parameter used to measure anticolesterol activity is the IC50 value. Antioxidant activity was evaluated by DPPH and Frap methods. The results showed that the ethanolic extract of bay leaves had an IC50 value of 49.50 ± 0.70 ppm. Ethanol extract of bay leaf showed antioxidant activity with IC50 value of 888.0835 ppm and equality of values with 1 mM FeSO4 of 295 ppm. Based on the results of the analysis, the anticholesterol activity of Bay leaf ethanol extract in inhibiting the HMG-CoA Reductase enzyme did not have a linear correlation with its antioxidant activity.
Uji Aktivitas Inhibitor Xanthin Oksidase dari Fraksi Ekstrak Etanol Herba Peperomia pellucida Prihastuti, Angelina Ajeng; Wijaya, Sumi; Hartanti, Lanny
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol. 4 No. 1 (2017)
Publisher : Faculty of Pharmacy, Widya Mandala Surabaya Catholic University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v4i1.2174

Abstract

Peperomia pellucida (L.) Kunth known as Suruhan is a potential medicinal plants, used traditionally to treat gout. Suruhan herb (Peperomia pellucida (L.) Kunth) had been studied in vivo, and found to be able to lowering uric acid levels in the blood. Ethanol extract of Peperomia pellucida (L.) Kunth herb also had been studied in vitro, and found to be potential to inhibit xanthine oxidase. The purpose of this study is to know the potential of fraction from ethanol extract of Peperomia pellucida (L.) Kunth. herb as xanthine oxidase inhibitor compared to allopurinol and its ethanolic extract. Ethanol extract of Peperomia pellucida (L.) Kunth herb was obtained by percolation method using ethanol 96%. The fractionation is done by column chromatography method using silica as stationary phase and n-hexane, n-hexane-ethyl acetate, ethyl acetate, ethyl acetate-ethanol, and ethanol as mobile phases. The fraction which contained flavonoid compounds was tested its xanthine oxidase inhibition potency using UV spectrophotometer at λ 290 nm. The absorbance was observed every 10 seconds for 10 minutes for extract and fraction with the concentration of 0.25 ppm - 5 ppm, while allopurinol was determined with the concentration of 0.2 ppm - 3.2 ppm. The result showed that ethyl acetate-ethanol fraction potentially inhibited xanthine oxidase with IC50 value of 5.00 ± 0.06 ppm, while ethanolic extract of Peperomia pellucida (L.) Kunth herb and allopurinol have IC50 value 0.33± 0.07 ppm and 0.84 ± 0.02 ppm respectively. Thus it can be concluded that ethyl acetateethanol fraction had potential as xanthine oxidase inhibitor, but the potential is lower than the ethanolic extract of Peperomia pellucida (L.) Kunth herb.
Co-Authors Anita Lie Bernadette Dian Novita Caesilia Putri Utami Caesilia Putri Utami, Caesilia Putri Diga Albrian Setiadi Dyana Sarvasti Emi Sukarti Emi Sukarti, Emi Evelyn Ongkodjojo Fandy Susanto Fandy Susanto, Fandy Felix Kurniawan Adithia Fenika Wulani Ferdinand, David Fransiskus Reynaldy Henry Kurnia Setiawan Ivan Priyantono Iwan Satria Djuanda Lisa Soegianto Marcellino Rudyanto Martha Ervina Prihastuti, Angelina Ajeng Setiawan, Henry K. Shavna Arfi Rasyid Sianty Dewi Sumi WIjaya, Sumi Tri Lestari Yonas, Stefani Maureen Kasih