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All Journal Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Pharmaciana: Jurnal Kefarmasian UNEJ e-Proceeding Jurnal Medik Veteriner Indonesian Journal of Pharmaceutical Research Jurnal Abdimas Kesehatan Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)

Martha Ervina

Fakultas Farmasi Universitas Katolik Widya Mandala Surabaya

Author-ID : 569816

Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Screening and Isolation of Cellulolytic Bacteria From Bagasse and Characterization of The Cellullase Produced Hartanti, Lanny; Susanto, Fandy; Utami, Caesilia Putri; Sukarti, Emi; Setiawan, Henry Kurnia; Ervina, Martha
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

Show Abstract | Download Original | Original Source | Check in Google Scholar

The research of screening and isolation of cellulase-producing bacteria from bagasse had been done. The objectives of the research were to obtain cellulolytic bacteria isolate, to determine the genus of the isolates and also to characterize the crude enzymes produced. One isolate with the highest activity was chosen to be purified further bystreak plate and pour plate methods in carboxyl methyl cellulose(CMC)Â agar media. The genus of the pure isolated bacteria was further characterized by macroscopic and microscopic properties, and biochemical assays using conventional methods and MicrobactTM Kit: gram-negative identification system (Oxoid). The result showed that the isolated cellulolytic bacteria had a close character similarity to Bacillus pumilus. The isolate was grown on MHB + CMC media with 2 hours of adaptation phase followed by logarithmic phase until 21 hours of incubation. Cellulase was started to be produced after 4 hours of fermentation and reached the highest activity at 21 hours of fermentation. The cellulase produced had the pH optimum at pH 5 and a temperature optimum at 60 Â°C, with optimum activity ranging from 0.0231 to 0.0264 U /ml. One unit of cellulase activity is defined as the amount of enzyme that will catalyze the production of 1 mg of glucose per minute at 37Â°C and pH 7.0. Keywords:Â bagasse, bacteria isolation, cellulolytic, biochemical characterization, enzyme characterization

Karakterisasi dan Uji Aktivitas Antibakteri dari Fungi Endofit Ranting Tanaman Manggis (Garcinia mangostana L.) terhadap Escherichia coli dan Staphylococcus aureus Widjaja, Kevin; Soegianto, Lisa; Ervina, Martha
Jurnal Farmasi Sains dan Terapan Vol 6, No 1 (2019)
Publisher : Jurnal Farmasi Sains dan Terapan

Resistance is commonly caused by improper usage of antibiotics, so the treatment of the infection always needs a new and more potent compounds. Endophytic microbes are microbes that all or part of its life stay in the tissues of plant, which established a mutually beneficial relationship between them. In this research, isolation of endophytic fungi was conducted by sticking the sterilized twigs of mangosteen to the media Malt Extract Agar obtained three pure endophytic fungi colonies. The antibacterial activity assay with direct inoculation method at media Plate Count Agar which was inoculated with bacteria and showed that the antibacterial activity against Escherichia coli ATCC 8739 was one isolate (ER2) with inhibition average ratio 1.42 and against Staphylococcus aureus ATCC 6538 was three isolates (ER1, ER2, and ER3) with inhibition average ratio 1.66; 1.37; and 1.89. The fungus were observed characteristics of the macroscopic, microscopic and biochemical assay. ER1 and ER3 isolates are suspected as a Penicillium genus with specific characteristics such as the conidiophore, fialid, metulae, and conidiospore. ER2 isolate was suspected as a Rhizoctonia genus because there are nearly right-angled hyphae and monilioid cells.

Penggunaan Carbormer 940 sebagai Gelling Agent dalam Formula Pasta Gigi Ekstrak Buah Apel (Malus sylvestris Mill) dalam Bentuk Gel Wijayanti, Luh Putu Widiasih; Darsono, Farida Lanawati; Ervina, Martha
Jurnal Farmasi Sains dan Terapan Vol 4, No 1 (2017)
Publisher : Jurnal Farmasi Sains dan Terapan

Teeth are often not protected from several diseases, despite having a hard structure. Dental diseases include dental caries (cavities) and diseases of the tooth supporting tissues. Dental caries is defined as a disease that results in damage to the hard tissue of the tooth structure through the process of demineralization. Streptococcus mutans is known as a major microorganism that plays a role in the process of dental caries. One way to reduce the formation of dental caries is to use toothpaste. Apples (Malus sylvestris Mill) Manalagi variety that has catechins, has efficacy as an anti dental caries. The purpose of this study was to formulate ethanol extract from apples as a gel toothpaste using carbomer 940 as a gelling agent. Apple ethanol extract used in this study was 9.6%, while the carbomer concentration of 940 used was 0.75% (formula I), 1.0% (formula II) and 1.5% (formula III). Evaluation of toothpaste formulas includes physical quality including organoleptic, homogeneity, pH, viscosity, dispersibility. Characteristic evaluation includes ease of release from the tube, adhesion and consistency. The stability evaluation includes room temperature stability, high temperature, and cycling tests. Evaluation of aseptability and evaluation of irritation is done in cow's jaws. Increasing the concentration of Carbomer 940 as a gelling agent has a significant effect on viscosity, dispersion, ease of release from the tube, adhesion and consistency. The test results show formula 3 (1.5% carbomer 940) is the best formula because it has produced physical quality in accordance with specifications, the resulting preparation is stable and very preferred and safe to use.

Efek Fraksi Air Daun Salam (Syzygium polyanthum) Terhadap Imunitas Alami Tikus Wistar Jantan Tamayanti, Wahyu Dewi; Soegianto, Lisa; Nurak, Elisabeth; Hadisoewignyo, Lannie; Ervina, Martha
Jurnal Farmasi Sains dan Terapan Vol 2, No 1 (2015)
Publisher : Jurnal Farmasi Sains dan Terapan

Pemberian fraksi air daun salam (Syzygium polyanthum) terhadap jumlah netrofil dan kadar IL-6 tjkus Wistarjantan yang telah diinduksi Staphylococcus aureus telah dilakukan. Tikus Wistar jantan dibagi dalam 3 ckelompok, masing-masing terdiri dari 5 ekor tikus, yaitu kelompok kontrol negatif (perlakuan NaCI 0,9%) kelompok uji (perlakuan fraksi air daun salam 200 mg/kg BB) dan ~elompok kontrol positif (perlakuan suspensi ibuprofen 400 mg/kg BB). Tikus dengan perlakuan dikorbankan satu jam setelah induksi Staphylococcus aureus, dan diambil darahnya. Perhitungan jumlah netrofil darah dilakukan .di bawah mikroskop dan penentuan kadar IL-6 dilakukan dengan metode ELISA. Hasil ana lisa data dengan anava satu arah mengindikasikan bahwa daun salam tidak menurunkan jumlah netrofil darah maupun IL-6 pada tikus Wistar jantan yang terinduksi Staphylococcus aureus.

Perbandingan Aktivitas Antibakteri 4-Metilpiperazin-N-Karbonil Ampisilin dengan Ampisilin Terhadap Micrococcus luteus dan Escherichia coli Pudjianto, Andreyanus Eko; Ervina, Martha; Caroline, Catherine; Soekardjo, Bambang; Foe, Kuncoro
Jurnal Farmasi Sains dan Terapan Vol 1, No 1 (2013)
Publisher : Jurnal Farmasi Sains dan Terapan

Piperacillin has antimicrobial action but it is not absorbed from gastrointestinal tract Therefore, it needs structure modification to increase its absorption while keeping its antimicrobial action. The 4-methylpiperazin-N-carbonyl ampicillin was the product of reaction between ampicillin and 4-methylpiperazin-N-carbonyl chloride using Schotten Baumann method. The aim of this research was to compare the antibacterial activity between 4-methylpiperazin-N-carbonyl ampicillin and ampicillin. The comparison test of antibacterial activity was done with Kirby-Bauer disk diffusion susceptibility method on Mueller-Hinton agar which had been inoculated with Micrococcus luteus and Escherichia coli. Based on orientation studies, concentration of 4-methylpiperazin-N-carbonyl ampicillin and ampiCillin were 0.5 ppm against Micrococcus luteus and 500 ppm against Escherichia collin order to get visible zone of inhibition. The results showed that 4-methylpiperazin-Ncarbonyl ampicillin had lower antibacterial activity than ampicillin which was showed by its zone of inhibition which was lower compared to ampicillin in inoculated agar media of Micrococcus luteus and Escherichia coli.

Screening and Isolation of Cellulolytic Bacteria From Bagasse and Characterization of The Cellullase Produced Lanny Hartanti; Fandy Susanto; Caesilia Putri Utami; Emi Sukarti; Henry Kurnia Setiawan; Martha Ervina
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

The research of screening and isolation of cellulase-producing bacteria from bagasse had been done. The objectives of the research were to obtain cellulolytic bacteria isolate, to determine the genus of the isolates and also to characterize the crude enzymes produced. One isolate with the highest activity was chosen to be purified further bystreak plate and pour plate methods in carboxyl methyl cellulose(CMC) agar media. The genus of the pure isolated bacteria was further characterized by macroscopic and microscopic properties, and biochemical assays using conventional methods and MicrobactTM Kit: gram-negative identification system (Oxoid). The result showed that the isolated cellulolytic bacteria had a close character similarity to Bacillus pumilus. The isolate was grown on MHB + CMC media with 2 hours of adaptation phase followed by logarithmic phase until 21 hours of incubation. Cellulase was started to be produced after 4 hours of fermentation and reached the highest activity at 21 hours of fermentation. The cellulase produced had the pH optimum at pH 5 and a temperature optimum at 60 °C, with optimum activity ranging from 0.0231 to 0.0264 U /ml. One unit of cellulase activity is defined as the amount of enzyme that will catalyze the production of 1 mg of glucose per minute at 37°C and pH 7.0. Keywords: bagasse, bacteria isolation, cellulolytic, biochemical characterization, enzyme characterization

Optimization of Coating Formula of White Pomegranate Peel Extract (Punica granatum L.) Film Coated Tablet Using Kollicoat Protect Stefani Luke; Martha Ervina; Lannie Hadisoewignyo
Indonesian Journal of Pharmaceutical Research Vol 1, No 1 (2021): Indo J Pharm Res 2021 1(1)
Publisher : Department of Pharmacy, Muhammmadiyah University of Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31869/ijpr.v1i1.1993

White pomegranate peel has many kinds uses, one of them as antimalaria. The unpleasant appearance and bitter taste of white pomegranate peel tablet can be covered by film coated using Kollicoat Protect. This research aimed to determine the effect of concentration and type of solvent used as a coating on the physical properties of the coated tablets and determine the optimum formula. Tablets were made using direct compression method and then coated using Kollicoat Protect. The optimization of coating formula was performed using a factorial design with design expert software ver 10.0. Responses used were tablet hardness, friability, disintegration time and increasing tablet mass. The results of this study showed that Kollicoat Protect cause increase of weight gain tablets and decrease the tablet friability, but prolong the disintegration time of tablets and decrease the tablet hardness while type of solvent can increase of weight gain tablets, tablet hardness, and decrease the tablet friability but prolong the disintegration time of tablets. The optimum combination formula was Kollicoat Protect at 13.6% and combination of water:alcohol (1:0.863) (w/v) with a theoretical response to weight gain of 2.340%; hardness 7.950 kp; friability 0.022%; and disintegration time 20.938 minutes.

In silico study of the essential oil compounds of ginger and thyme on Coronavirus-2 receptors Caroline Caroline; Martha Ervina; Muhammad Rizki Fadhil Pratama; Maria Anabella Jessica
Pharmaciana Vol 13, No 3 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i3.26964

Coronavirus-2 (SARS-Cov-2) is a virus that attacks the respiratory system and causes the Covid-19 pandemic. After the pandemic, prevention and appropriate therapy research continue to be carried out to anticipate the emergence of more dangerous viruses. In line with the culture of consuming herbs that has arisen due to the effects of the pandemic, in this study, an insilico screening was carried out for essential oil compounds produced by ginger and thyme herbs which have been widely consumed by the public. The aim of the research was to find the essential oil content that has the most potential as an antiviral against coronavirus-2. The research method was carried out in silico, including ligand preparation, receptor and method validation, and analysis of ligand-receptor binding interactions using the AutoDoc 4.2.6 program. As a comparison, a study was conducted on remdesivir and favipiravir, which have been used as antivirals. The three components that have the most potential based on the calculation of the free energy value, were determined by the ADMET parameters using the Admet lab 2.0 program. The results showed that the three components in the essential oil exhibited better interactions when compared to remdesivir and favipiravir at the 3-Cl protease and spike glycoprotein receptors. The results of the insilico study and ADMET prediction test showed that of the three most potent compounds, lamda-farnesen was the most potent and safe to use

Layanan Telemedicine Terintegratif Dokter dan Apoteker bagi Penderita Covid-19 di Lingkungan Paroki Kristus Raja Surabaya Caroline Caroline; Senny Yesery Esar; Martha Ervina; Wuryanto Hadinugroho; Steven Steven; Yudhikuari Sincihu; Laura Wihanto
Jurnal Abdimas Kesehatan (JAK) Vol 4, No 3 (2022): November
Publisher : Sekolah Tinggi Ilmu Kesehatan Baiturrahim Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36565/jak.v4i3.388

The global pandemic related to the coronavirus (2019-nCoV) continues to hit the world, including Indonesia until the end of 2021. The efforts to improve health services were carried out by various parties, including by members of the King of Christ Parish church as part of the Surabaya community. From this parish in July 2021, 301 people were sick with COVID-19 and almost 11% of them died. The public and health services were panicked by the occurrence of a wave of viral mutations, with the increasing of sufferers and high mortality rates. Integrated telemedicine services between doctors and pharmacists are one of the best options to prevent transmission and severity to support patient recovery rates. With telemedicine, health care needs are individualized and patient development is more controlled. The method was carried out through direct consultation between doctors, pharmacist, and patients, using the whatsapp call platform. Monitoring of patients was continued by pharmacist using the whatsapp message platform until the patient was cured. If necessary, consultation with the doctor could be carried out again during the patient’s healing process. The research show that there is an integration of doctor and pharmacist services in telemedicine for COVID-19 patients in diagnosing, determining the best supporting drugs and therapies for the patients. Quantitatively, there was a decrease in the mortality rate, an increase in patient compliance and recovery. As an additional indicator, the residents welcome the integrated services that are carried out and can be developed for other health services.

Optimized RT-qPCR Detection of Hepatic Lipopolysaccharide-Binding Protein in Diet-Induced Obese Mice Tjahjono, Yudy; Wedharga, I Gede Putu Adhi; Novita, Bernadette Dian; Tahalele, Paul; Wijaya, Hendy; Soediono, Endang Isbandiati; Hendrata, Adi Pramono; Dewi, Sianty; Wijaya, Sumi; Ervina, Martha; Kuncorojakti, Suryo
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.308-317

High-throughput RT-qPCR results on hepatic lipopolysaccharide-binding protein (LBP) expression in obese subjects are essential, as they reveal the endotoxin’s role in the development of obesity and non-communicable disease (NCD). This study aimed to optimize RT-qPCR detection of LBP in diet-induced obese mice. This study primarily focused on addressing high variability through reference gene normalization. A total of six male C57BL/6 mice aged 6 weeks were randomly allocated into two dietary treatments (n = 3), consisting of mice fed with the standard chow diet (SCD group) and mice fed with the high-fat and high sucrose diet (HFHS group) ad libitum for 8 weeks. Relative quantification strategies involving the standard 2-ΔΔCt method (calibrator as mean) and the modified 2-ΔΔCt method (calibrator as individual sample-matched biological replicates) were compared in terms of their variability. Obesity was successfully induced in the HFHS treatment group, as indicated by significantly higher body weight, calorie intake, and LBP relative expressions compared to the SCD group. In addition, a sample-specific calibrator approach using the modified 2-ΔΔCt method resulted in lower variability in relative gene expression levels. A modified 2-ΔΔCt method, which utilizes a sample-specific calibrator to counteract sample-specific variability, was successfully employed to address high variability in RT-qPCR results.

Co-Authors Anita Maya Sutedja Caesilia Putri Utami Caesilia Putri Utami, Caesilia Putri Caroline Caroline Caroline Caroline Catherine Caroline Emi Sukarti Emi Sukarti, Emi Esar, Senny Yesery Fandy Susanto Fandy Susanto, Fandy Farida Lanawati Darsono Foe, Kuncoro Handayani, Yuliastuti Ika Hendrata, Adi Pramono Henry Kurnia Setiawan Kuncorojakti, Suryo Lannie Hadisoewignyo Lannie Hadisoewignyo Lanny Hartanti Laura Wihanto Lisa Soegianto Maria Anabella Jessica Mohammad Rizki Fadhil Pratama Novita, Bernadette Dian Nurak, Elisabeth Paul L Tahalele Pudjianto, Andreyanus Eko Senny Yesery Esar Sianty Dewi Soediono, Endang Isbandiati Soekardjo, Bambang Stefani Luke Steven, . Sumi WIjaya, Sumi Tjahjono, Yudi Tjahjono, Yudy Wahyu Dewi Tamayanti, Wahyu Dewi Wedharga, I Gede Putu Adhi Widjaja, Kevin Widjaja, Kevin Wijaya, Hendy Wijayanti, Luh Putu Widiasih Wuryanto Hadinugroho Wuryanto Hadinugroho Yudhikuari Sincihu

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