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All Journal Jurnal Sain Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Livestock and Animal Research Jurnal Kedokteran Hewan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
RISZA HARTAWAN
Indonesia Research Center for Veterinary Science Indonesian Agency for Agricultural Research and Development Jalan R.E. Martadinata No. 30, PO Bx 52 Bogor 16114, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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IN SILICO ANALYSIS OF SMALL INTERFERING RNA TARGETING THE NUCLEOPROTEIN GENE OF INFLUENZA VIRUSES Hartawan, Risza; Ratnawati, Atik; Sendow, Indrawati; Dharmayanti, Ni Luh Putu Indi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8521

Abstract

Small interfering RNA (siRNA) is a promising therapeutic against viral infection, includ-ing Influenza viruses. However, the Influenza viruses have massive variants with high mutation rates. Therefore, the siRNAs could be futile against newly emerging viruses. Thus, this study aimed to analyze siRNAs targeting the nucleoprotein gene of Influen-za viruses. Using bioinformatic analyses, the siRNAs were simulated against 5 sub-types of Influenza viruses, including H1N1, H3N2, H5N1, H7N9, and H9N2. Bioinfor-matic tools for the folding structure of messenger RNA were utilized to select effective siRNA. As a result, 32 siRNA sequences targeting the nucleoprotein gene were identi-fied. The precision medicine concept seems applied to the siRNA treatment for the In-fluenza virus since each siRNA is effective in its respective virus target. Based on the nucleotide mismatch parameter, most siRNA does not have coverage for the multiple infections of all five subtypes of Influenza viruses, except for NP1089 and NP1496. Later, the secondary and tertiary structure analysis of messenger RNA demonstrated that siRNA has different circumstances in its RNA target position. Therefore, siRNA mapping based on the RNA folding structure approach provides a tool for selecting more effective sequences against Influenza virus infection. Both siRNA NP1089 and NP1496 were predicted to have similar effectivity in knocking down Influenza virus in-fection. Moreover, the cocktail application of siRNA treatment may be effective as an alternative strategy in matching co-infection of multiple Influenza virus subtypes.
Karakterisasi Kultur Virus African Swine Fever Sampel Lapang Indonesia Menggunakan Kultur Sel Primer Leukosit Babi Ratnawati, Atik; Hartawan, Risza; Sendow, Indrawati; Assadah, Nur Syabiq; Nuradji, Harimurti; Dharmayanti, Ni Luh Putu Indi; Wibawan, I Wayan Teguh; Mayasari, Ni luh Putu Ika
Jurnal Sain Veteriner Vol 43, No 3 (2025): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.109976

Abstract

African Swine Fever (ASF) merupakan penyakit viral yang sangat menular dan mematikan pada babi, yang disebabkan oleh virus African Swine Fever (ASF). Sejak tahun 2018, penyakit ini telah menyebar dan menyebabkan konsekuensi sosial ekonomi yang besar terhadap industri babi di beberapa negara Asia, termasuk China, Vietnam, dan Indonesia. Penelitian ini bertujuan untuk melakukan isolasi virus ASF dari sampel lapang menggunakan kultur sel primer leukosit babi, identifikasi karakteristik hasil propagasi virus ASF secara in vitro dan respon sel leukosit terhadap inokulasi virus ASF. Sel leukosit dikoleksi dari darah babi donor yang sehat dan dikultur dalam medium plasma homolog. Propagasi dilakukan dengan menginokulasikan sampel lapang dengan kode Indonesia/2022/Pig/PSJ ke kultur sel primer leukosit babi yang konfluen (70-80%). Pengamatan morfologi sel dilakukan dengan menggunakan mikroskop cahaya setiap 24, 48, 72, 96,120, 144, and 164 jam pasca inokulasi (jpi). Sampel lapang dari kultur sel primer leukosit babi dipurifikasi dengan menggunakan metode Percoll. Pelet virus di deteksi virus ASF dengan menggunakan uji qPCR. Hasil penelitian menunjukkan adanya perubahan morfologis pada sel primer leukosit yang terinfeksi, dengan adanya reaksi hemadsorpsi (HAD) yang teramati pada 48 jpi, dibandingkan dengan sel kontrol yang tidak terinfeksi. Pengikatan eritrosit babi ke permukaan sel yang terinfeksi virus ASF, membentuk rosette-like structure. Reaksi hemadsorpsi (HAD) dapat diamati setelah 2 kali blind passage. Purifikasi virus ASF menggunakan Percoll dapat meningkatkan kemurnian virus yang ditandai dengan nilai Ct yang lebih rendah dibandingkan dengan supernatant hasil kultur sel primer leukosit babi.
Retrospective analysis of Marek's disease virus outbreak in 2013 on chicken farms in North Sumatra, Indonesia Hartawan, Risza; Nurjanah, Diana; Suyatno, Teguh; Purwany, Any; Ratnawati, Atik; Indriani, Risa; Sendow, Indrawati; Dharmayanti, Ni Luh Putu Indi
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.78210

Abstract

Objective: Marek's disease is lymphoma in chicken caused by Marek's disease virus serotype 1 (MDV-1), characterized by paralysis and tumour formation. The disease has caused enormous economic loss worldwide, including in Indonesia. Unfortunately, molecular data related to MDV-1 from Indonesia is limited. Therefore, this study aimed to identify genetic characteristics of MDV-1 causing outbreaks in commercial farms in North Sumatra, Indonesia, 2013.Methods: The amplification of Meq, pp38, gC, and gE genes of the strain MDN2013 was carried out using the PCR protocol. Fragment gene sequencing was accomplished using the Sanger method. Subsequently, the bioinformatic analyses of BLASTN and phylogenetic tree were applied to analyze the molecular characteristics of these gene sequences.Results: The Meq, pp38, gC, and gE genes of the strain MDN2013 were successfully sequenced and submitted to the NCBI GenBank database. The Meq genes share a high identity with Asian viruses, mainly those originating from Indonesia, India, and China. The pp38, gC, and gE genes share similarities with other viruses in more diverse regions in Asia, Africa, Europe, and America. The phylogenetic tree analysis of the Meq gene demonstrated specific patterns for pathotype biodiversity where the strain MDN2013 has been categorized as virulent up to very virulent virus.Conclusion: In conclusion, the molecular approach is an effective tool for investigating the MDV-1 biodiversity in the field, especially the Meq gene sequence analysis.
Co-Authors Assadah, Nur Syabiq Atik Ratnawati DharmayantI NLPI Farida Syamsiah, Farida I wayan Teguh Wibawan Indi Dharmayanti, Ni Luh Putu Indrawati Sendow J. Meer JOANNE MEERS K. Robinson KARL ROBINSON Mayasari, Ni Luh Putu Ika NI LUH PUTU INDI DHARMAYANTI NLP Indi Dharmayanti NLPI Dharmayanti Nuradji, Harimurti Nurjanah, Diana Purwany, Any RISA INDRIANI Suyatno, Teguh T. Mahony TIMOTHY MAHONY Ukhti, Dwi Rillah