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Muhaimin Rifa'i
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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ETHANOL EXTRACTS OF PROPOLIS (EEP) AGAINST LYMPHOCYTE ACTIVATION CELLS IN HEALTHY MICE (Mus Musculus) BALB/C Emi Rohmawati; Muhaimin Rifa'i
Biotropika: Journal of Tropical Biology Vol 2, No 4 (2014)
Publisher : University of Brawijaya

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Abstract

Propolis is a substance like glue formed by honey bees from resin of plant which has the ability to stimulate immune system. The purpose of this study is to determine the ethanol extract of propolis on the activity of lymphocytes in healthy Balb/c mice and to asses the optimum dose administration of EEP for lymphocyte activation in Balb /c mice. Methods: mice was aclimate for two weeks, mice control without treatment EEP and a other was treated with EEP dependent dose, dose  1 (50 mg/ kgBW),  dose 2 (100 mg/kgBW), and dose 3 (200 mg/kgBW). Spleen was isolated and to find out the amount of lymphocyte we analyzed with flow cytometry. Parameter measured in this experiment is quantitative by measuring relative number of T cells that consist of CD4+CD62L+, CD4+CD62Lˉ, CD8+CD62L+, dan CD8+CD62Lˉ. Then data was analyzed by SPSS 16.0 software for windows with ANOVA test and advanced by Tukey test and Gomes-Howell with an interval 0.05 is used. The result showed that ethanol extract of propolis can activate CD4 T cells so that CD4 T cell lost CD62L molecule and turned into T cells CD4+CD62Lˉ. Ethanol extract of propolis can enhance proliferation of naïve type of CD8 T cell, so that the number of T cell memory (TCM) decreased. Dose of 100 mg/kgBW of ethanolic propolis extract spatially act as immunostimulant for CD4+CD62Lˉ activation, while the dose of 200 mg/kgBW act as  immunosuppressant in the same cells. Key words: T cell activation,  propolis, Balb/c  mice.
Efect of Bitter Melon (Momordica charantia) and Bitter (Andrographis paniculata) Extract to Humoral Immunity in Balb/c Mice Model of Type 2 DM Lailiya Vina Rochmatika; Muhaimin Rifa'i
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

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ABSTRACT Type 2 diabetes mellitus (DM2) is disease caused by decreased insulin receptor sensitifity,  so that, causing level blood glucose to rise. One of strategy to reduce blood glucose level is by administration with medicinal herbs. The aim of this research was to analyze the effect of bitter melon (Momordica charantia) and bitter (Andrographis paniculata) extract on B220 cell in Balb/c mice model of type 2 DM. Mice in the age of 5 days were divided into five groups, each group consists of 4 mice. The treatments consist of negative control (normal mice), positive control (DM2), dose 1, dose 2, and dose 3. DM2 was induced by intraperitoneally injection of Streptozotocin (STZ) in neonate BALB/c mice. Treatment with bitter melon and bitter was performed for 14 days. Blood glucose level was examined by glucometer. Spleen cells were isolated and analyzed by flow cytometry post treatment. The result showed that dose 3 of extract of Bitter (Andrographis paniculata) and Bitter melon (Momordica charantia) can increase the number of B cells in DM2 treated mice compare to DM2 untreated mice. Furthermore, we showed that Bitter melon extract herbal (Momordica charantia) and Bitter (Andrographis paniculata) had an ability to reduce blood glucose level in concentration dependent manner. Dose 3 known as the most efective to optimize either B cells proliferation or blood glucose reduction. Key words: Bitter, bitter mellon, diabetes mellitus type 2, streptozotocin
Activity of Dexamethasone Therapy on Pro-Inflammatory Cytokines Profile of Balb/c Mice with Biliary Atresia Qonitatul Khasanah; Muhaimin Rifa'i
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
Publisher : University of Brawijaya

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ABSTRACT Biliary atresia is a neonatal obstructive cholangiopathy that progresses to end-stage liver disease. Dexamethasone is one of synthetic glucocorticoid which has function as an anti-inflammatory agent. Here, we investigated whether dexamethasone could modulate the immune activity in mice strain Balb/c with biliary atresia based on the change of quantity of IFN-γ and TNF-α as a pro-inflammatory molecules. This study consists of 2 stages. The first stage is pre-condition which is made the biliary duct become fibrosis by injecting 20 µl of phosphate buffered saline containing 1.5 x 106 fluorescence-forming units Rhesus Rotavirus (RRV) subcutaneously on first day (24 hours) after the mice was born. The second stage is injection with dexamethasone with dose 0,5 mg/kg BW subcutaneously on the 7th-14th day and 14th-21st day. The clinical effect of dexamethasone is investigated on 14th and 21st day by flow cytometry method. Data were analyzed using Kruskal-Wallis test (p<0,05) and Mann-Whitney test using SPSS 16 for Windows. Rotavirus injection subcutaneously was proven to stimulate the production of proinflammatory cytokines, especially in the third week of termination. The result indicated an increasing number of proinflammatory cytokines such as IFN-γ and TNF-α after RRV injection but after injection of dexamethasone the number of those cytokines is decreased. It can be understood that dexamethasone has a capability to reduce the effect of inflammation regard to the decrease of proinflammatory cytokines. Keywords : Biliary atresia, Dexamethasone, Inflammation, Obstructive, Rhesus Rotavirus
Bioactivity of Proplis to the CD4 + and CD8+ T cells Producing IFN-γ Cytokines in BALB / C Mice Yonna Ayundria; Muhaimin Rifa&#039;i
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
Publisher : University of Brawijaya

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Abstract

ABSTRACT Propolis (bee glue) is a natural resinous product of honey bees which collected from exudates and plant buds, rich in biochemicals constituents including mostly flavonoids, phenols and various acids bond. These compound are believed to be responsible as immunomodulatory agents. The study aims to determine the immunomodulatory activity of ethanolic extract of propolis to the CD4+ and CD8+ T cells producing IFN-γ cytokines and analyze the differences immune responses between control and treatment group by in vivo. Stages include animal acclimation for ± 1 week, preparation of Ethanolic Extracts of Propolis / EEP, Oral Administration with doses levels of 0 mg / kg BW; 50 mg / kg BW (DI); 100 mg / kg BW( DII); 200 mg / kg BW (DIII) for 2 weeks, isolation of lymphocyte cells from spleen, flowcytometry analysis to asses cell number and surface molecule expression. Data was analyzed using Kruskal Wallis Test with α = 0,05 and followed by Mann Whitney Test by SPSS 16.0 for windows with complete randomized design. The results showed that a dose of 50 mg / kg BW was increases the relative number of CD8+ T cells producing IFN-γ cytokines significantly (p <0.05) compared with controls. However, at the same dose the relative number of CD4+ T cells producing IFN-γ cytokines was decreased significantly (p<0,05). Based on this case, its dose supposedly that the ethanolic extract of propolis play role in maintaining the balance or homeostatic of IFN-γ cytokines production by T cell subsets. Dose of 100 mg/kg BW and 200 mg / kg BW could decrease the relative number of activated CD4+ T cells producing IFN-γ cytokine significantly compared to controls. Keywords: CD4+ T cells, CD8+ T cells, ethanolic extract of propolis, IFN-γ cytokines, in vivo
Profile of T cells after dexamethasone treatment in BABL/c mice Iga Dwi Januarisasi; Muhaimin Rifa&#039;i
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

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ABSTRACT Regulation of homeostatic mechanisms and the development of T lymphocytes is influenced by several things, one of them is glucocorticoids. Dexamethasone is a synthetic glucocorticoid drugs used in the treatment for several diseases acted as an anti-inflammatory. The aim of this experiment was to confirm the effect of dexamethasone at normal dose and high dose on T cell profile and also to know the quantity of T cells after dexamethasone injection in each dose. This experiment we applied 18 mice in the age of 2 week and divided into 3 treatment groups with six replication i.e. control, dexamethasone injection with normal dose (0.5 mg/kg BW) and high dose (10 mg/kg BW), then observed on day-7 after injection. T cells were isolated from the spleen and analyzed by flow cytometry. Data analysis was confirmed with the ANOVA test followed by Turkey test with significance different (α) of 0.05. The result showed that dexamethasone act as immunosuppressant agent on high dose (10 mg/kgBB). Dexamethasone injection with normal dose on healthy mice showed no significant different in total number of CD4+, CD8+, CD4+CD62L, and CD8+CD62L compared to control. But, dexamethasone injections with high dose showed that the total number of CD4+, CD8+, CD4+CD62L, and CD8+CD62L were decreased significantly. Key words: Dexamethasone, Flowcyto metry, Glucocorticoid, Immunosuppressant, T lymphocyte.
Co-Authors Aris Soewondo Aulanni'am, Aulanni'am Churi Wardah Deasy Luandayanti Emi Rohmawati Firda Agustin Ganys Tri Silvana Iga Dwi Januarisasi Lailiya Vina Rochmatika Moch Sasmito Djati Ovi Ardiana Qonitatul Khasanah Riza Rahmawati septi utami dewi Uwais Al Qarni Wira Eka Putra Yonna Ayundria Yuyun Ika Christina