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All Journal Jurnal Bioleuser Biotropika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Agrotek UMMat Kenanga : Journal of Biological sciences and Applied Biology Jurnal Kelautan dan Perikanan Indonesia Jurnal Medika Veterinaria
Diannita Harahap
Universitas Islam Negeri Ar-Raniry Banda Aceh
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary

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Viabilitas Bakteri Asam Laktat Kombucha Terenkapsulasi: Review Diannita Harahap
Jurnal Bioleuser Vol 2, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/kanun.v%vi%i.12005

Abstract

Teh fermentasi kombucha memiliki ragam mikroorganisme (mikroba) bermanfaat bagi tubuh manusia. Berkenaan dengan fungsi utama sebagai penyeimbang flora normal dalam tubuh, minuman ini prospektif menjadi minuman kesehatan dan menyegarkan tubuh. Selain melibatkan Bakteri Asam Asetat (BAS) dan juga kelompok khamir Saccharomyces pada prosesnya, minuman ini juga melibatkan Bakteri Asam Laktat (BAL).  Sediaan teh dalam bentuk serbuk dinilai cukup ekonomis dibandingkan sediaan cair. Idealnya teh fermentasi kombucha juga dapat diproduksi dalam bentuk sediaan serbuk, dapat dinikmati pada kondisi seduhan air panas maupun dingin (es) serta tetap baik pada kondisi saluran cerna. Tantangannya adalah bagaimana viabilitas sel tetap terjaga pada kondisi tersebut diatas. Salah satu cara mempertahankan viabilitas sel adalah dengan enkapsulasi menjadi hal yang penting. Penerapan teknik enkapsulasi sel BAL dapat mempertahankan pertumbuhan dan aktivitas metaboliknya.
Characterization and Antibacterial Activity of Endophytic Bacteria from Flesh Fruit of the Arecaceae Family Against Antibiotic Resistant Bacteria Escherichia coli Diannita Harahap
Biotropika: Journal of Tropical Biology Vol 9, No 2 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2021.009.02.11

Abstract

Bacteria can symbiotic mutualism and synergize with plants in the tissue and do not cause harm to plants. On the other hand, bacteria resistance provides an opportunity to search for antibacterials from natural material without threatening plant biodiversity. The purpose of this study was to characterize and test the antibacterial activity of endophytic bacteria from the fruit of the Arecaceae family against antibiotic-resistant bacteria Escherichia coli. The method used was direct culture on the growth of media in the laboratory by first sterilizing the surface of the flesh of the samples, such as Aren (Arenga pinnata), Coconut (Cocos nucifera), and Sago (Metroxylon sagu). The pure isolates obtained were characterized according to Bergeys Manual for Identification by performing Gram stain, catalase, and coagulase test. The results obtained were five isolates with codes A1, A2, K, S1, and S2 with variation in morphology colonies. Gram staining, catalase, and coagulase verification of the five isolates were groups of Grampositive cocci, catalase-positive, and coagulase-negative bacteria. Based on tests carried out on the characters of the endophytic bacteria, it was obtained a close relationship with the genus Staphylococcus. The results of the inhibitory activity test showed that Staphylococcus sp. K with the highest criteria of 43.26 mm and Staphylococcus sp. A1 with the lowest inhibition criteria with a value of 24.73 mm. Both are categorized as very strong inhibition criteria.
ISOLASI BAKTERI PENGIKAT NITROGEN DENGAN MENGGUNAKAN MEDIA JENSEN Feizia Huslina; Diannita Harahap
Jurnal Agrotek Ummat Vol 6, No 2 (2019): August
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.415 KB) | DOI: 10.31764/agrotek.v6i2.1238

Abstract

Bakteri pengikat nitrogen adalah jenis bakteri yang paling banyak ditemukan di tanah dan sebagiannya bersimbiosis dengan akar tumbuhan. Bakteri ini mengikat nitrogen di udara dan mengubahnya menjadi nitrat yang kemudian digunakan oleh tumbuhan. Media Jensen adalah media selektif yang umumnya digunakan untuk menumbuhkan bakteri pengikat nitrogen. Penelitian ini bertujuan untuk mengisolasi bakteri pengikat nitrogen dengan menggunakan media Jensen. Bakteri diisolasi dari rizosfer di sekitar UIN Ar-Raniry, Banda Aceh dan dilakukan karakteristik morfologi dan Optical Density (OD). Empat isolat (IS-A, IS-B, IS-C, IS-D) ditemukan pada serial pengenceran 10-2 dan 10-4 dan isolat D (IS-D) memiliki nilai Optical Density (OD) tertinggi yaitu sebesar 0,373.
KARAKTERISASI FUNGI SELULOTIK DARI SERASAH DAUN MANGROVE ASAL HUTAN MANGROVE LAMNGA KABUPATEN ACEH BESAR Syafrina Sari Lubis; Marzha Faradilla; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol 2 No 2 (2022): Oktober 2022
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v2i2.2382

Abstract

Desa Lamnga Kabupaten Aceh Besar merupakan salah satu desa yang terletak di pesisir pantai dan merupakan kawasan hutan mangrove. Serasah daun mangrove merupakan penyumbang utama biomassa selulosa di lingkungan pesisir laut yang dibantu oleh mikroorganisme. Tujuan dari penelitian ini adalah untuk mengetahui karakteristik jamur selulolitik dari serasah daun mangrove dan mengetahui potensi enzim selulolitik yang dihasilkan oleh jamur selulolitik. Pengambilan sampel dilakukan secara acak dengan titik pengambilan berdasarkan lokasi ditemukannya serasah daun mangrove. Sampel serasah daun dimasukkan ke dalam media CMC dan diinkubasi selama 7 hari pada suhu 25-300C. Didapatkan empat jenis jamur selulolitik yaitu genus Aspergillus sp, Aspergillus niger, Penicillium, dan Culvularia sp. Hasil uji kemampuan degradasi selulosa diperoleh nilai indeks ≥11 , kategori sedang dengan kode isolat FS3, FS8, FS9, dan nilai indeks ≤11 , kategori lemah dengan kode isolat FS1, FS2, FS4, FS5, FS6 , FS7 dan FS10.
TOTAL MIKROBA DAN BAKTERI ASAM LAKTAT TEH FERMENTASI KOMBUCHA Diannita Harahap
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 38, No 3 (2021)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2021.38.3.1198

Abstract

Teh kombucha merupakan minuman fermentasi dengan bahan dasar teh dan gula. Dalam proses pembuatannya melibatkan konsorsium mikroorganisme (mikroba) bakteri dan khamir. Disamping itu, keberadaan Bakteri Asam Laktat (BAL) menjadi salah satu kriteria  pangan dikategorikan mengandung probiotik. Tujuan penelitian ini untuk menghitung total mikroba, total BAL dan melihat kesesuaian dengan syarat angka probiotik yang diterima di Indonesia. Adapun parameter yang diujikan yaitu total mikroba, total BAL dan pH selama waktu fermentasi. Penelitian ini merupakan penelitian deskriptif. Metode yang digunakan untuk menghitung total mikroba dan total BAL yaitu Angka Lempeng Total (ALT). Pengujian terhadap sampel dilakukan pada rentang waktu fermentasi 0, 8, 11 dan 14 hari selama empat kali masa produksi berulang. Hasil penelitian menunjukkan total mikroba tertinggi pada hari ke-14 yaitu 3.8 x 106 koloni/mL dan total BAL sebesar 3.4 x 106 koloni/mL. Teh kombucha yang diujikan memenuhi syarat menjadi minuman probiotik dengan syarat total BAL > 106 koloni/mL yang diterima di Indonesia dan total mikroba dengan nilai total > 106 koloni/mL.
AKTIVITAS KITOSAN LIMBAH CANGKANG KERANG BULU (Anadara antiquata) TERHADAP Aeromonas hydrophila PADA IKAN NILA (Oreochromis niloticus) Tuti Aulia; Syafrina Sari Lubis; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol 2 No 2 (2022): Oktober 2022
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

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Abstract

Aquaculture in Indonesia is carried out in freshwater, brackish and marine waters with limited production of several fish species such as tilapia. Although tilapia is easy to adapt to the environment, it can be infected with Aeromonas hydrophila bacteria. One of the efforts to treat bacteria is to use chitosan which is a chitin derivative that can be developed as an antimicrobial candidate because it contains lysozyme enzymes and aminopolysaccharide groups that can inhibit microbial growth. The purpose of this study was to determine the activity of chitosan from feather shell waste (Anadara antiquata) in inhibiting Aeromonas hydrophila in vitro and in vivo in tilapia. The methods used for the manufacture of chitosan include deproteination, demineralization, depigmentation, and deacetylation. In vitro testing was conducted to test the antibacterial activity using the disc diffusion method (Kirby-Bauer test). In vivo testing was carried out by the immersion method. The results showed that the chitosan yield was 77.2%. The most effective concentration of chitosan antibacterial activity against Aeromonas hydrophila was at 7% with an inhibition zone value of 6.85 mm. The results of data analysis showed that there was a significant difference between the test treatments. The in vivo test on tilapia showed that the highest percentage was found in the optimal concentration treatment (7% chitosan) with a value of 68.74%. The results of data analysis showed that there was no significant difference between optimal concentration treatment and positive control.
EKOENZIM SEBAGAI PENGENDALIAN PATOGEN Fusarium sp. DAN Colletotrichum sp. PADA TANAMAN PISANG BARANGAN (Musa acuminata) Burdah Asni; Syafrina Sari Lubis; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol 3 No 1 (2023): April 2023
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

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Abstract

Barangan banana (Musa acuminata) belongs to Musaceae family which has hig economic value and has the potential as a commercial material and food diversification. One of the diseases of Barangan banana (Musa acuminata) which causes quite high damage is Fusarium sp. wilt disease. and anthracnose disease Colletotrichum sp. This study aims to control Fusarium sp. and Colletotrichum sp. using ecoenzymes in this study ecoenzymes were made from fruit peel waste which was fermented for three months. Testing the activity of controlling Fusarium sp. and Colletotrichum sp. using the diffusion method. Ecoenzymes phytochemical test results contained alkaloids and saponins. Ecoenzymes at concentrations of 10% - 40% showed strong and very strong activity against Fusarium sp. while Colletotrichum sp. 20% concentration shows very strong activity.
KEMAMPUAN Pseudomonas aeruginosa PAO1 DALAM SERAPAN LOGAM BESI (Fe) PADA LIMBAH LINDI DI TPA GAMPONG JAWA KOTA BANDA ACEH Diannita Harahap; Zakirul Rahmad; Husnawati Yahya; Juliansyah Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol 3 No 1 (2023): April 2023
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

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Abstract

Leachate is a liquid containing hazardous materials such as metal Iron (Fe). One method that can be used to reduce Fe levels in leachate is using the bioremediation method. This study aimed to determine the absorption ability of Pseudomonas aeruginosa bacteria in remediating Fe as well as the physical and chemical characteristics of leachate such as COD, TSS, and pH before and after using P. aeruginosa. This study used an experimental method of four treatments and three replications. The concentration of bacteria used was 108 CFU/ml with ex-situ contact times of 0, 10, 20, and 30 hours. The results showed that the highest decrease in Fe, COD, TSS, and pH occurred at 30 hours. The Fe value can be reduced to 4.45 mg/L with an absorption effectiveness of 38.19%; the initial COD value of 1.432 mg/L decreased to 1.301 mg/L. In addition, the TSS value decreased to 213 mg/L from the initial level of 223 mg/L and the highest pH decreased, namely 9.6 to 8.1. The uptake of P. aeruginosa bacteria is good in reducing acceptable levels of Fe and pH.
POTENSI ENZIM SELULASE DARI BAKTERI TERMOFILIK DI KAWASAN WISATA IE SUUM KABUPATEN ACEH BESAR Rahmatil Majidah; Syafrina Sari Lubis; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol 3 No 1 (2023): April 2023
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

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Abstract

Thermophilic bacteria are a group of bacteria that grow at a temperature range of 45oC to 80oC and have the potential to produce cellulase enzymes. The tourist area of Ie Suum, Aceh Besar District is one of the hot springs in Aceh. This study aims to observe the morphological characteristics, cellulase enzyme potential and activity test in producing cellulase enzymes from thermophilic bacteria found in Ie Suum. The method of isolating thermophilic bacteria uses the pour plate method and testing the cellulase enzyme activity using the DNS (3,5-dinitrosalicylic Acid) method. 15 isolates were obtained, namely 13 Gram positive and 2 Gram negative, 2 genera were obtained, namely the genus Bacillus sp. and the genus Pseudomonas sp. Based on the potential of the cellulase enzyme, the 15 isolates showed the largest clear zone diameter, namely isolate TS6 3,67 cm, isolate TS7 2,33 cm, and isolate TS10 4,00 cm. The results of measuring enzyme activity using the DNS method obtained isolates TS6 4,7 x 10-3 U/mL, isolates TS7 4,4 x 10-3 U/mL, and isolates TS10 5,5 x 10-3 U/mL.
Profil Endoparasit pada Ikan Gabus (Channa striata) Berdasarkan Kondisi Habitat Dina Meltia; Diannita Harahap; Rizki Nanda; Nanda Rizki Purnama
Jurnal Kelautan dan Perikanan Indonesia Vol 2, No 1: April (2022)
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jkpi.v2i1.24548

Abstract

Profil endoparasit pada ikan Gabus (Channa striata) berdasarkan kondisi habitatnya. Infeksi endoparasit berpotensi mengakibatkan ganguan pertumbuhan hingga kematian ikan. Infeksi endoparasit menganggu sistem metabolisme tubuh inang dengan merusak organ pencernaan seperti lambung dan usus. Ikan Gabus juga mengandung Cu, Fe, Ca dan Zn yang bermanfaat mempercepat pembentukan sel baru dalam penyembuhan luka pasca operasi maupun luka bakar. Tujuan penelitian ini untuk mengetahui prevalensi, intensitas, dominansi, hubungan panjang bobot, dan Faktor kondisi ikan gabus berdasarkan kondisi habitatnya. Penelitian ini dilakukan Laboratorium Multifungsi Biologi Uin Ar-raniry. Penelitian dilaksanakan pada bulan April 2021. Hasil penelitian menunjukkan terdapat 3 jenis endoparasit yang menginfeksi ikan gabus yaitu Pallisentis sp, Anisakis sp dan Camallanus sp. prevalensi endoparasit yang paling tinggi terdapat pada areal selokan senilai 76,67 % (Usually/biasanya) dan prevalensi palig rendah didapatkan pada areal rawa 63, 3 % (Frequently/sangat sering), intensitas paling rendah terdapat pada areal sawah senilai 8,1 (sedang)  dan paling tinggi pada areal rawa senilai 9,32 (sedang). Dominansi endoparasit yang paling banyak ditemukan Anisakis sp dan pallisentis sp mendominansi ke 3 habitat tersebut sedangkan Camallanus Cuma terdapat pada habitat sawah dan selokan saja. Ikan gabus yang terinfeksi cenderung memiliki panjang bobot rata-rata lebih rendah dibandingkan ikan yang sehat. Ikan gabus dengan kisaran panjang 218,5-228,5 dan 258,5-268,5 mm serta berat 146,2-176,2 dan 177,2-207,2 cenderung terhadap infeksi endoparasit dibandingkan ukuran lainnya.
Co-Authors Burdah Asni Burdah Asni Darmawi Darmawi Dina Meltia Feizia Huslina Husnawati Yahya Husnawati Yahya Ismi Mauliasari Juliansyah Harahap, Juliansyah Marzha Faradilla Marzha Faradilla Nanda Rizki Purnama Rahmatil Majidah Rahmatil Majidah Rizki Nanda Syafrina Sari Lubis Tuti Aulia Tuti Aulia Zakirul Rahmad Zakirul Rahmad Zikrina Rahmi