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All Journal Jurnal Bioleuser Biotropika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Agrotek UMMat Kenanga : Journal of Biological sciences and Applied Biology Jurnal Kelautan dan Perikanan Indonesia Jurnal Medika Veterinaria
Diannita Harahap
Universitas Islam Negeri Ar-Raniry Banda Aceh
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary

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22. The Effect Of Giving White Pure Ethanol Extract (Allium sativum) On The Growth Of Aeromonas hydrophyla Bacteria In Goldfish (Cypirinus carpio) cultivation Zikrina Rahmi; Diannita Harahap; Syafrina Sari Lubis; Darmawi Darmawi
Jurnal Medika Veterinaria Vol 13, No 2 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i2.12356

Abstract

 This study aimed to determine the effect of garlic extract (Allium sativum) to the growth of Aeromonas hydrophila bacteria. Sample of A. hydrophila was obtained from the Fish Quarantine Station of the Aceh Fisheries Product Quality and Safety Control.Extraction of garlic was conducted by maceration method using ethanol solvent. This study used 6 treatments and consisted of three replications. Treatment I concentration of garlic extract 45%, treatment II with concentration of garlic extract 50%, treatment III concentration 55%, treatment IV concentration 60%. Positive control used chloramphenicol antibiotics and negative control used 96% ethanol. The test results showed that 60% garlic extract (A. sativum) was more effective in inhibiting the growth of A. hydrophila bacteria compared to other concentrations. The lowest effect was owned by 45%. Ethanol extract of garlic (A. sativum)had the ability to inhibit the growth of A. hydrophila because it has antibacterial compounds.
KARAKTERISASI FUNGI SELULOTIK DARI SERASAH DAUN MANGROVE ASAL HUTAN MANGROVE LAMNGA KABUPATEN ACEH BESAR Syafrina Sari Lubis; Marzha Faradilla; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 2 No. 2 (2022): Oktober 2022
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v2i2.2382

Abstract

Desa Lamnga Kabupaten Aceh Besar merupakan salah satu desa yang terletak di pesisir pantai dan merupakan kawasan hutan mangrove. Serasah daun mangrove merupakan penyumbang utama biomassa selulosa di lingkungan pesisir laut yang dibantu oleh mikroorganisme. Tujuan dari penelitian ini adalah untuk mengetahui karakteristik jamur selulolitik dari serasah daun mangrove dan mengetahui potensi enzim selulolitik yang dihasilkan oleh jamur selulolitik. Pengambilan sampel dilakukan secara acak dengan titik pengambilan berdasarkan lokasi ditemukannya serasah daun mangrove. Sampel serasah daun dimasukkan ke dalam media CMC dan diinkubasi selama 7 hari pada suhu 25-300C. Didapatkan empat jenis jamur selulolitik yaitu genus Aspergillus sp, Aspergillus niger, Penicillium, dan Culvularia sp. Hasil uji kemampuan degradasi selulosa diperoleh nilai indeks ≥11 , kategori sedang dengan kode isolat FS3, FS8, FS9, dan nilai indeks ≤11 , kategori lemah dengan kode isolat FS1, FS2, FS4, FS5, FS6 , FS7 dan FS10.
AKTIVITAS KITOSAN LIMBAH CANGKANG KERANG BULU (Anadara antiquata) TERHADAP Aeromonas hydrophila PADA IKAN NILA (Oreochromis niloticus) Tuti Aulia; Syafrina Sari Lubis; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 2 No. 2 (2022): Oktober 2022
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v2i2.3033

Abstract

Aquaculture in Indonesia is carried out in freshwater, brackish and marine waters with limited production of several fish species such as tilapia. Although tilapia is easy to adapt to the environment, it can be infected with Aeromonas hydrophila bacteria. One of the efforts to treat bacteria is to use chitosan which is a chitin derivative that can be developed as an antimicrobial candidate because it contains lysozyme enzymes and aminopolysaccharide groups that can inhibit microbial growth. The purpose of this study was to determine the activity of chitosan from feather shell waste (Anadara antiquata) in inhibiting Aeromonas hydrophila in vitro and in vivo in tilapia. The methods used for the manufacture of chitosan include deproteination, demineralization, depigmentation, and deacetylation. In vitro testing was conducted to test the antibacterial activity using the disc diffusion method (Kirby-Bauer test). In vivo testing was carried out by the immersion method. The results showed that the chitosan yield was 77.2%. The most effective concentration of chitosan antibacterial activity against Aeromonas hydrophila was at 7% with an inhibition zone value of 6.85 mm. The results of data analysis showed that there was a significant difference between the test treatments. The in vivo test on tilapia showed that the highest percentage was found in the optimal concentration treatment (7% chitosan) with a value of 68.74%. The results of data analysis showed that there was no significant difference between optimal concentration treatment and positive control.
KEMAMPUAN Pseudomonas aeruginosa PAO1 DALAM SERAPAN LOGAM BESI (Fe) PADA LIMBAH LINDI DI TPA GAMPONG JAWA KOTA BANDA ACEH Diannita Harahap; Zakirul Rahmad; Husnawati Yahya; Juliansyah Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 3 No. 1 (2023): April 2023
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v3i1.3118

Abstract

Leachate is a liquid containing hazardous materials such as metal Iron (Fe). One method that can be used to reduce Fe levels in leachate is using the bioremediation method. This study aimed to determine the absorption ability of Pseudomonas aeruginosa bacteria in remediating Fe as well as the physical and chemical characteristics of leachate such as COD, TSS, and pH before and after using P. aeruginosa. This study used an experimental method of four treatments and three replications. The concentration of bacteria used was 108 CFU/ml with ex-situ contact times of 0, 10, 20, and 30 hours. The results showed that the highest decrease in Fe, COD, TSS, and pH occurred at 30 hours. The Fe value can be reduced to 4.45 mg/L with an absorption effectiveness of 38.19%; the initial COD value of 1.432 mg/L decreased to 1.301 mg/L. In addition, the TSS value decreased to 213 mg/L from the initial level of 223 mg/L and the highest pH decreased, namely 9.6 to 8.1. The uptake of P. aeruginosa bacteria is good in reducing acceptable levels of Fe and pH.
POTENSI ENZIM SELULASE DARI BAKTERI TERMOFILIK DI KAWASAN WISATA IE SUUM KABUPATEN ACEH BESAR Rahmatil Majidah; Syafrina Sari Lubis; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 3 No. 1 (2023): April 2023
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v3i1.3119

Abstract

Thermophilic bacteria are a group of bacteria that grow at a temperature range of 45oC to 80oC and have the potential to produce cellulase enzymes. The tourist area of Ie Suum, Aceh Besar District is one of the hot springs in Aceh. This study aims to observe the morphological characteristics, cellulase enzyme potential and activity test in producing cellulase enzymes from thermophilic bacteria found in Ie Suum. The method of isolating thermophilic bacteria uses the pour plate method and testing the cellulase enzyme activity using the DNS (3,5-dinitrosalicylic Acid) method. 15 isolates were obtained, namely 13 Gram positive and 2 Gram negative, 2 genera were obtained, namely the genus Bacillus sp. and the genus Pseudomonas sp. Based on the potential of the cellulase enzyme, the 15 isolates showed the largest clear zone diameter, namely isolate TS6 3,67 cm, isolate TS7 2,33 cm, and isolate TS10 4,00 cm. The results of measuring enzyme activity using the DNS method obtained isolates TS6 4,7 x 10-3 U/mL, isolates TS7 4,4 x 10-3 U/mL, and isolates TS10 5,5 x 10-3 U/mL.
UJI KEPEKAAN ANTIBIOTIK TERHADAP BAKTERI Salmonella sp. PADA JAGUNG BAKAR DI KAWASAN ULEE LHEU BANDA ACEH Ismi Mauliasari; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 3 No. 2 (2023): Oktober 2023
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v3i2.4207

Abstract

Jagung bakar merupakan salah satu jajanan yang digemari masyarakat. Dalam proses pengolahannya rentan terkontaminasi mikroba karena alat dan bahan yang digunakan kurang bersih dan lain sebagainya. Penelitian ini bertujuan untuk menguji resisten antibiotik pada Salmonella sp. pada jajanan jagung bakar di kawasan wisata Ulee Lheu Kota Banda Aceh. Sampel menggunakan 7 jagung bakar oles bumbu (JO) dan 7 jagung bakar tidak oles bumbu (JTO). Pengujian resisten antibiotik menggunakan metode Kirby-Bauer. Hasil uji resistensi isolat JO pada Amoxicillin 69% dan Kloramfenikol 46% resisten. Hasil isolat JTO pada Amoxicillin 50% dan Kloramfenikol 50% resisten.Dilanjutkan dengan uji statistik Anova diketahui pada isolat JO perbandingan antar kedua antibiotik dengan nilai sig. > 0,05 (0,053 > 0,05) maka tidak terdapat perbedaan rata-rata zona hambat dan perbandingan antar isolat JO 1 sampai JO 13 dengan nilai sig. < 0,05 (0,000 < 0,05) maka terdapat perbedaan rata-rata diameter zona hambat. Sedangkan pada isolat JTO perbandingan antar kedua antibiotik dengan nilai sig.< 0,05 (0,000 < 0,05) maka nilai tersebut terdapat perbedaan rata-rata zona hambat dan perbandingan antar isolat JTO 1 sampai dengan JTO 8 dengan nilai sig.< 0,05 (0,000 < 0,05) maka nilai tersebut terdapat perbedaan rerata zona hambat.
ISOLASI DAN UJI AKTIVITAS ENZIM SELULASE PADA BAKTERI SELULOLITIK DARI SERASAH AKASIA (Acacia mangium Willd.) Tazkiatun Nufus; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 5 No. 2 (2025): Oktober 2025 (In Progress)
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v5i2.8483

Abstract

Cellulase is an enzyme with high demand in the industrial sector and can be extracted from cellulolytic bacteria. These bacteria are capable of producing cellulase and hydrolyzing cellulose into simple products such as glucose. The content of acacia leaves consists of 50.77% cellulose, hemicellulose, and lignin. This study aimed to determine the characteristics, identification, and enzymatic activity of cellulolytic bacteria isolated from acacia litter (Acacia mangium Willd.). The methods used in this research were both quantitative and qualitative, involving the isolation of cellulolytic bacteria from acacia litter using selective CMC media by the spread plate method, clear zone testing using the Kirby–Bauer method, and enzyme activity assays. Eight cellulolytic bacterial isolates (BS1, BS2, BS3, BS4, BS5, BS6, BS7, and BS8) with different morphological characteristics were obtained. Based on macroscopic, microscopic, and biochemical identification, the isolates were identified as belonging to the genera Cellulomonas sp. (BS1 and BS3), Pseudomonas sp. (BS2, BS4, BS5, BS6, and BS8), and Corynebacterium sp. (BS7). The two isolates showing the highest clear zone indices on CMC media were BS1 with an IS value of 0.90 and BS7 with an IS value of 0.74. The cellulase enzyme activity of isolate BS1 (Cellulomonas sp.1) was 0.0041 U/mL with a specific activity of 0.0032 U/mL, while that of isolate BS7 (Corynebacterium sp.) was 0.0049 U/mL with a specific activity of 0.0019 U/mL. Keywords: cellulase enzyme; cellulolytic bacteria; Acacia mangium Willd.
Co-Authors Burdah Asni Burdah Asni Darmawi Darmawi Dina Meltia Feizia Huslina Husnawati Yahya Husnawati Yahya Ismi Mauliasari Juliansyah Harahap, Juliansyah Marzha Faradilla Marzha Faradilla Nanda Rizki Purnama Rahmatil Majidah Rahmatil Majidah Rizki Nanda Syafrina Sari Lubis Tazkiatun Nufus Tuti Aulia Tuti Aulia Zakirul Rahmad Zakirul Rahmad Zikrina Rahmi