Garuda - Garba Rujukan Digital

Hardi Julendra

Balai Pengembangan Proses dan Teknologi Kimia (BPPTK)-LIPI, Yogyakarta

Author-ID : 293246

Computer Science & IT Veterinary

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The use of earthworm meal (Lumbricus rubellus) as anti-pullorum agent in feed additive of broiler chicken Damayanti, Ema; Sofyan, Ahmad; Julendra, Hardi; Untari, Tri
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

The aim of this research was to study the use of earthworm meal (TCT) L. rubellus as anti pullorum agent in poultry feed additive (IP). The antibacterial activity of TCT against Salmonella pullorum was examined using diffusion agar method at each of the following concentrations: 0, 25, 50, 75 and 100% (w/v) in 100 ÂµL DMSO. In vivo test was conducted using 80 broiler chicken and were infected by S. pullorum with treatments of: IP0: IP contained 0% TCT, IP1: IP contained 25% TCT, IP2: IP contained 50% TCT, IP3: IP contained 75% TCT and IP4: IP contained 100% TCT. Each treatment was replicated 4 times with 4 chicks each. Feed additive was periodically fed to broiler during 7 days before and 10 days after infection. Anti-pullorum activities were evaluated using serology test, isolation and biochemical identification of S. pullorum. The results showed that 75% TCT was optimum to inhibit S. pullorum in vitro. The isolation and identification of S. pullorum results showed that 0 out of 8 (0%) broilers treated with IP4 was not infected by S. pullorum whereas 1 out of 2 (50%) broilers treated with IP0 were infected by S. pullorum. The reduction of S. pullorum prevalence as followed by increasing TCT in feed additive. In conclusion, TCT as poultry feed additive could inhibit S. pullorum infection. Key words: Earthworm Meal, Feed Additive, S. Pullorum

Antibacterial activity and retained protein of earthworm meal (Lumbricus rubellus) as feed additive combined with chitosan Sofyan, Ahmad; Damayanti, Ema; Julendra, Hardi
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 3 (2008)
Publisher : Indonesian Animal Sciences Society

This research was conducted to enhance the bacterial growth inhibition of E. coli by using earthworm (Lumbricus rubellus) meal (TCT) which was added with chitosan and its effect on the retained protein in broilers. Inhibition of E. coli growth was tested using dilution method on the nutrient broth by additional 2% TCT combined with 0, 0.5, 1.0 and 1.5% chitosan. Retained protein was measured using broiler fed diet containing 2% TCT (w/w) and added by chitosan 0, 0.5, 1.0 and 1.50% of TCT (w/w). The numbers of 15 broilers Cobb strain 35 days old were arranged on Completely Randomized Design (CRD). Results showed inhibition of E. coli was increased using TCT mixed chitosan. The highest inhibition to E. coli growth obtained from TCT + 0.5% chitosan. Retained protein tended to increase up to 1% (w/w) chitosan. Otherwise, chitosan level more than 1% could reduce protein retention. It is concluded that use of 1% chitosan increased TCT capability to inhibit E. coli and protein retention in the broilers. Key Words: Feed Additive, Chitosan, L. rubellus, E. coli

PROFIL ASAM AMINO EKSTRAK CACING TANAH (LUMBRICUS RUBELLUS) TERENKAPSULASI DENGAN METODE SPRAY DRYING Hayati, Septi Nur; Herdian, Hendra; Damayanti, Ema; Istiqomah, Lusty; Julendra, Hardi
Teknologi Indonesia Vol 34 (2011)
Publisher : LIPI Press

Antibiotics in poultry were widely used as growth promotor and anti-infective. When antibiotics were used continuously for long periods, it caused microbial resistance. Various attempts were made to replace antibiotics along with the increasing of trend towards demand for healthy, safe and free from harmful residues of livestock products. One of them was application of earthworm which contains high protein and complete amino acids. Earthworms were extracted into water extract form by decoct method. Water extract form had some weaknesses. It was necessary to formulate into a solid dosage form which was more stable, reproducible, and practical. The selected formulation was encapsulation by spray drying method. Quality control of feed additives can be seen from biological value. Biological value correlated positively with amino acids equilibrium or Essential Amino Acid Index (EAAI). The result showed that earthworm extract had both essential and non-essential amino acids higher than earthworm meal and encapsulated earthworm extract. The highest essential amino acid in earthworm meal was isoleucine (3.14%), in earthworm extract was lysine (8.16%), and in encapsulated earthworm extract was leucine (1.71%). The highest non-essential amino acids in earthworm meal and encapsulation of earthworm extract were glutamic acid, respectively 7.67% and 1.87%, while the earthworm extracts was serine (14.52%). The high value of IAAE in earthworm extract showed that the extraction method resulted better amino acid balance (69.87%) than earthworm meal (58.67%). While the value IAAE of encapsulated earthworm extract showed that levels of essential amino acid balance was lower (16.05%) than the earthworm meal (69.87%) and the earthworm extract (58.67%).

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