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All Journal Jurnal Sain Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences JURNAL BIOLOGI INDONESIA
Dyah Ayu Hewajuli
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Biochemistry, Genetics & Molecular Biology Veterinary

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SIRKULASI VIRUS AVIAN INFLUENZA H5N1 TAHUN 2010 : VIRUS GENETIC DRIFT MIRIP A/CK/WEST JAVA/PWT-WIJ/2006 DITEMUKAN DI BEBERAPA KABUPATEN DI SUMATRA DAN JAWA Dharmayanti, NLP Indi; Ratnawati, Atik; Hewajuli, Dyah Ayu; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3069

Abstract

The avian influenza H5N1 virus circulation in 2010 : Genetic Drift Like Virus A/Chicken/West Java/Pwt-Wij/2006 was found in several districts of Sumatra and Java. Until 2011, the H5N1 subtype of AI virus is still circulating in many parts of Indonesia. The discovery of the AI viruses which have undergone genetic drift since 2006 until now requires serious attention from the government in terms of AI disease control, the surveillance and monitoring of virus circulation and execution of genetic mapping to determine the genetic character of the AI virus at the molecular level, especially on the surface of glycoproteins (HA and NA protein).This information is needed to determine the diversity and character of the AI virus in Indonesia. Genetic data are used to evaluate the strategy to control AI in Indonesia, such as vaccination and the vaccine seed used and determine the extent of AI virus mutation in Indonesia has beenmutated. This study conducted by monitoring of the AI virus circulation throughout 2010. The methods used were AI virus isolation, RT-PCR, sequencing of genes coding for viral surface and the prediction of three-dimensional analysis to determine the location of virusmutation. The results of this study showed that most of the AI virus subtype H5N1, which was isolated during the year 2010, showed similar mutations to the genetic drift virus in 2006, A /Ck/West Java/Pwt-Wij/2006. The viruses were characterized by the presence of 18-19 amino acid substitutions at the level of the HA protein. On the NA protein level, there is a single mutation which was buried in the NA molecule. This mutation probably did not influence for NA activity. Genetic mapping of AI virus subtype H5N1 in 2010 showed that the viral genetic driftas the mutan virus A/Ck/West Java/Pwt-Wij/2006 have circulated not only in West Jawa alone but has been found on the island of Sumatra, Banten, West Jawa and East Jawa.Keywords: Circulation, avian influenza H5N1 virus, genetic drift
Identification of Bovine Rotavirus Group A in Bogor, West Java Hewajuli, Dyah Ayu; Pratama, Yuda; Winarsongko, Agus; Purwani, Ani; Suyatno, Teguh; Fabeane, Ajeng; Ermayati, Ermayati; Nuradji, Harimurti; Assadah, Nur Sabiq; Endrawati, Dwi; Ratnawati, Atik; Saepulloh, Muharram; Dharmayanti, NLP Indi
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90683

Abstract

Abstrak  Diare merupakan penyakit yang menyebabkan angka kesakitan yang tinggi pada pedet dan kematian neonatal. Penyakit ini dapat disebabkan oleh beberapa agen penyakit yang berbeda. Rotavirus Grup A (RVA) atau Bovine Rotavirus merupakan salah satu agen infeksi penyebab diare pada pedet. Selanjutnya, diare neonatal pada pedet dapat berdampak pada kerugian ekonomi bagi ternak sapi perah dan sapi potong di seluruh dunia karena menyebabkan gangguan pertumbuhan, meningkatnya biaya perawatan, dan/atau kematian pada hewan sakit. Prevalensi Bovine Rotavirus dapat berbeda antar negara di seluruh dunia. Sirkulasi Bovine Rotavirus pada sapi telah dilaporkan di beberapa negara tetapi sirkulasi Bovine Rotavirus pada sapi di Indonesia belum diketahui. Untuk mengetahui prevalensi Rotavirus grup A atau Bovine Rotavirus (BRV), 100 sampel feses dikoleksi dari pedet dengan gejala klinis diare atau tidak diare di Kabupaten Bogor, Jawa Barat pada tahun 2021. Sampel dianalisis terhadap urutan yang mengkode protein kapsid bagian dalam VP6 (subkelompok) menggunakan Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Lima dari 100 sampel feses sapi (5%) terdeteksi positif BRV. Pada penelitian ini menunjukkan bahwa kelompok Rotavirus atau Bovine Rotavirus (BRV) telah bersirkulasi di antara ternak sapi di Indonesia, khususnya Kabupaten Bogor. Sampel positif  Rotavirus grup A atau Bovine Rotavirus (BRV) dapat diidentifikasi dengan metode diagnosis dini (RT-PCR). Kata kunci : Bovine Rotavirus; RT PCR; Bogor Abstract              Diarrhea is the most disease that cause high morbidity in calves and neonatal mortality. This disease can be caused by several different infectious agents. Group A rotaviruses (RVA) or Bovine Rotavirus are one of the infectious agents causing diarrhea in calves. Then, Neonatal calf diarrhea can impact to economic losses to dairy and beef cattle herds worldwide, in consequence of growth disorders, value of treatment, and/or death of sick animals. The prevalence of Bovine Rotavirus can become different in the worldwide. The circulation of these bovine rotavirus in calves from the some region has already been demonstrated but the circulation of bovine rotavirus in Indonesia is not known. To investigate the prevalence of A group Rotaviruses or Bovine Rotavirus (BRV), 100 fecal samples were collected from calves with diarrhea or no diarrhea in Bogor district, West Java at 2021. The samples were analyzed for sequences encoding the inner capsid protein VP6 (subgroup) using RT-PCR. Five of 100 specimens of bovine fecal (5%) were detected positive as BRV positive. In this study, A group Rotaviruses or Bovine Rotavirus (BRV) have been circulated among cattle herds in Indonesia, particularly Bogor District. The positive samples of A group Rotaviruses or Bovine Rotavirus (BRV) can be identified using the early diagnosis method (RT-PCR). Keywords : Bovine Rotavirus; RT PCR; Bogor District
Co-Authors Assadah, Nur Sabiq Atik Ratnawati Dwi Endrawati Ermayati, Ermayati Fabeane, Ajeng N.L.P Indi Dharmayanti NI LUH PUTU INDI DHARMAYANTI Ni Luh Putu Indi Dharmiayanti NLP I Dharmayanti NLP Indi Dharmayanti NLPI Dharmayanti Nuradji, Harimurti Purwani, Ani RISA INDRIANI Saepulloh, Muharram Suyatno, Teguh Winarsongko, Agus yuda pratama