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Journal : Jurnal Ilmu Ternak Veteriner

The susceptibility differences of buffalo and Ongole calves against trickle infection with Fasciola gigantica Ening Wiedosari; S Widjajanti; S Partoutomo
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 1 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.133 KB) | DOI: 10.14334/jitv.v3i1.95

Abstract

A pen trial was carried out in order to determine the susceptibility differences of a trickle infection with Fasciola gigantica in buffalo and Ongole calves. Treated animals were infected orally with 15 metacercariae of F. gigantica twice weekly for 32 weeks and were slaughtered at 36 weeks. The results showed that buffalo calves had significantly lower fluke burdens than Ongole calves (P<0 .01) . All of the infected Ongole calves had fasciola eggs in their faeces 18 weeks after the commencement of infection. In contrast, eggs were detected only in 3 out of 7 infected buffalo calves at week 20, in 4 at week 28 and in 6 at week 30. Faeces of the seventh buffalo remained free of eggs until week 36. Rates of growth were reduced by 25%and 10,3% in infected Ongole and buffalo calves respectively . Circulating blood eosinophilia ofboth hosts, ahallmark of helminth infections, increased following infection, but values in buffaloes was greater than Ongole calves mainly in week 4 and 8 after infection (P<0.01). These results might be concluded that the susceptibility of buffalo calves to trickle infection with F. gigantica was lower compared to Ongole calves.   Keywords : Fasciola gigantica; susceptibility, buffalo calves, Ongole calves
Infection of Parainfluenza type 3 (PI-3) as one of the causative agent of pneumonia in sheep and goats Indrawati Sendow; Tatty Syafriati; Ening Wiedosari; Paul Selleck
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 1 (2002): MARCH 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.275 KB) | DOI: 10.14334/jitv.v7i1.276

Abstract

Serological survey was conducted to obtain the prevalence of Parainfluenza type 3 (PI-3) reactor as one of the causative agent of pneumonia in sheep and goats in abatoir at Jakarta and some small holder farms in Indonesia. Serological test using serum neutralization from 724 goat sera and 109 sheep sera indicated that only 1% of goats were serologically reactors and none of sheep sera had antibodies against PI-3 virus. Isolation of the virus from 56 bronchus and trachea swab and 345 lungs indicated that only one sampel from lung showed cythopathic effect (CPE) in Madin Darby Bovine Kidney (MDBK) cell lines identification of the virus using serum neutralization test indicated that the virus neutralized reference PI-3 antisera. The isolate came from one lung (7%) of 24 that showed histopathologically pneumonia intertitialis that usually caused by viral infection. Key words: Parainfluenza type 3 (PI-3), virus isolation, sheep, goat, pneumonia intertisialis
The activities of antioxidant enzymes extracted from Fasciola gigantica infecting Thin Tailed and Merino sheep Ening Wiedosari
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.434 KB) | DOI: 10.14334/jitv.v11i2.520

Abstract

Previous studies shown that Indonesian Thin Tailed (ITT) sheep are more resistant to Fasciola gigantica infection than Merino sheep. This difference could be mediated by intrinsic defense enzymes of the parasite. Certain enzymes are known to be crucial in parasite survival against host-derived immune responses. We measured some of them to identify if any comparative differences between the enzyme activities of the parasites from the two hosts (ITT & Merino sheep) could account for the mechanisms of parasite resistance to killing by the Merino host and susceptibility to killing by the ITT host. Parasites were extracted from the liver of infected ITT and Merino sheep and superoxide dismutase (SOD), glutathione S-transferase (GST) and catalase (CAT) enzyme activities were assayed. SOD and GST levels were found to be higher in parasites isolated from Merino than those of ITT sheep (P<0.05), CAT activity was not detected in any of the parasites. There was significantly higher eosinophils (P<0,05) in the ITT sheep peritoneal cells. These results suggested that SOD dan GST are important molecules in determining susceptibility in Fasciola-infected Merino sheep and resistance in Fasciola-infected ITT sheep. Key Words: Fasciola Gigantica, Indonesian Thin Tailed Sheep, Merino Sheep, Superoxide Dismutase (SOD), Glutathione S-Transferase (GST)
Pathology and clinicopathology of buffalo against trickle infection with Fasciola gigantica Ening Wiedosari
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 2 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (688.472 KB) | DOI: 10.14334/jitv.v3i2.107

Abstract

Eleven male buffalo calves were divided into infected group of 7 animals and non-infected control group of 4 animals. The infected group was then inoculated with trickle doses of 15 Inetacercariae of Fasciola gigantica twice weekly for 32 weeks and killed 36 weeks after first infection. There were no clinical symptoms observed . Infected and non-infected buffaloes, had similar values of packed cell volume, haemoglobin and red blood cell counts . In infected buffaloes, plasma glutamate dehydrogenase enzyme activity increased in proportion to the dregree of hepatocyte destruction level and evidence of necrosis caused by the migrating of immature flukes through the parenchyma prior to their entry into the bile ducts. While the values of plasma glutamyl transpeptidase showed only a minimal rise with a small peak in week 20 as evidenced by histological observation that infected caused limited damage to epithelial surface of the bile duct . These results indicates that, the resistance mechaninisms of buffalo against fasciolosis infection occurred in the liver or before flukes entering into the bile ducts. These results seem to indicate that, in buffalo, resistance mechanisms to fasciolosis infection occured in the liver or before flukes entry into the bile ducts.   Key words: Fasciola gigantica, clinicopathology, buffalo calves, trickle infection, resistance