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Diding HP
Faculty of Medicine, Sebelas Maret University
Medicine & Pharmacology

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Pengaruh Probiotik terhadap Gambaran Histologis Mukosa Usus pada Mencit Balb/C Model Alergi HP, Diding; Listyaningsih S., Endang; Subijanto, AA
Jurnal Kedokteran YARSI Vol 16, No 1 (2008): JANUARI - APRIL 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v16i1.219

Abstract

The mechanisms by which probiotic bacteria affect the immune system are unknown yet, but many of them are attributed to an increase in the innate or in the acquired immune response. The aim of this study was to examine the effect of the probiotic on the histology structure of intestinal layer in a mice model of allergy. Male Balb/C mice were sensitized and challenged intra peritoneally (i.p) with ovalbumin(OVA). Mice were immunized i.p. on days 0 and 7 with 2.5 mg of OVA adsorbed to 7.75ml of Aluminum hydroxide gel. OVA challenges (10 mg in l0 ml of PBS) were administered orally on days 8, 9, 10, 11, 12 and 13, with mice being sacrificed on day 14. This probiotic was orally administered to Balb/C mice. Intestinal microscopic slides were prepared using parafin method and stained with hematoxylin eosin staining. The data were analyzed by Kruskal-Wallis, followed by MannWhitney Test. The results showed that probiotic can minimized inflammation of intestinal layer in the experimental animal, not significantly different with 3rd generation of antihistamine.
Pengaruh Ekstrak Daun Ceremai (Phyllanthus acidus [L.] Skeels) terhadap Kadar IgE pada Mencit Model Alergi Subijanto, AA; HP, Diding
Jurnal Kedokteran YARSI Vol 16, No 1 (2008): JANUARI - APRIL 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.868 KB) | DOI: 10.33476/jky.v16i1.220

Abstract

It was reported in previous studies that pharmacological activities were identified in various compounds extracted from Phyllanthus Acidus (PA) such as anti-inflammatory, antilipoxigenase, anti-allergic and cyclooxigenase inhibitor. It is also acknowledged that Ig E serum level is normally low and usually increase in allergic conditions. This study was aimed to determine the effect of PA leaf extract on Ig E level in mice model of allergic asthma. Male Balb/C mice were sensitized and challenged intraperitoneally (i.p) with ovalbumin(OVA). Mice were immunized i.p. on days 4 and 20 with 2.5 mg of OVA adsorbed to 7.75ml of Aluminum hydroxide gel. OVA challenges (10 mg in l0 ml of PBS) were administered aerosolly on days 26, 29, 32, 39 and 46, with mice being sacrificed on day 47. Ig E level in serum was determined using ELISA kits (Alpha Diagnostic, USA) according to the manufacturer's instructions. A one-way analysis of variance (ANOVA) with LSD post hoc analysis was used to determine significant differences. Results were expressed as mean + SEM, and values of p < 0.05 were considered statistically significant. The results showed that Ig E level in the negative control group was 50.13+33.40 ng/ml, PA leaf extract was 51.17+26.15 ng/ml, and positive control group was 48.97+33.50 ng/ml while OVA group was 152.89+58.00 ng/ml (p >0.05). It was concluded that PA leaf extract could minimize Ig E level, not significantly different with 3rd generation of antihistamine.
Co-Authors AA Subijanto Devina Noviani Pramono Listyaningsih S., Endang Riani Dwi Hastuti Sri Hartati Sri Hartati Hadinoto