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Biosorption of Lead(II) using Trichoderma viride in the Aqueous Solution Rensani Taloin; Anna Safitri; Sasangka Prasetyawan; Budi Kamulyan; Ulfa Andayani
The Journal of Pure and Applied Chemistry Research Vol 7, No 1 (2018): Edition January-April 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.361 KB) | DOI: 10.21776/ub.jpacr.2018.007.01.399

Abstract

Lead(II) is considered as the main cause of pollutant that is toxic, corrosive, and irritant. One method that can be applied for reducing Pb(II) in the environment is by using microorganisms. In this work, the study of biosorption of Pb(II) in the water samples was conducted using Trichoderma viride. The research is focused on determination of optimum conditions including pH, biosorption time, and initial concentration of Pb(II) used. The profiles in functional groups contained in the T. viride have been monitored using FT-IR spectrophotometry. Results showed that the maximum biosorption of Pb(II) achieved at pH 4.5, with equilibrium of contact time of 20 h, optimum concentration of 50 mg/L, and adsorption capacity of 85 mg/1x106 T. viride colonies. The FTIR results indicated that biosorption process changed the functional groups in the T. viride. These have shown in the absorption bands at ~3200 cm-1, ~2850 cm-1, ~2260 cm-1, ~1650 cm-1,  ~1450 cm-1, 1180 cm-1, and in the finger printing regions. The biosorption mechanism was proposed through the adsorption process between positively charged metal ions and the negative charge on the functional groups, such as -COO-, -OPO32-, and –NH2-, on the cell surface.
Phytochemical Profile and Pharmacological Activity of Local Velvet Bean (Mucuna pruriens) of Timor Tefa, Azor Yulianus; Nino, Jefrianus; Ceunfin, Syprianus; Taloin, Rensani; Yani, Ahmad
Ahmar Metastasis Health Journal Vol. 4 No. 4 (2025): Ahmar Metastasis Health Journal
Publisher : Yayasan Ahmad Mansyur Nasirah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/amhj.v4i4.463

Abstract

The processing of velvet beans (Mucuna pruriens) from Timor is traditionally done by boiling them repeatedly twelve times before consumption due to concerns about their antinutrient content. On the other hand, earlier research indicated that velvet beans possess bioactive compounds that may serve as antioxidant and antidiabetic agents. The purpose of this study was to identify the bioactive components in extracts of velvet beans grown by the Timorese community, assess the overall levels of secondary metabolite compounds, and evaluate their bioactivities through in vitro antioxidant and hyperglycemia tests. Velvet beans samples were extracted using maceration, soaking them in ethanol and n-hexane solvents, followed by qualitative phytochemical analysis. Additionally, quantitative determination of secondary metabolites was performed, along with pharmacological activity tests, which included assessing antioxidant activity and antidiabetic effects through the spectrophotometric method. The findings revealed that the local Timor velvet beans extract contained flavonoid compounds at a concentration of 12.77 ± 3.10 mg QE/g, while the levels of phenolic and tannin compounds in the extract could not be quantified due to an absorbance lower than the y-intercept. The DPPH test demonstrated that the antioxidant activity of the local Timor velvet beans extract was measured at 1,964.51 ppm, which is categorized as very weak in comparison to the standard ascorbic acid solution that registered a value of 5.39, classified as very strong. The antidiabetic assessment indicated that the extract could inhibit the activity of the α-glucosidase enzyme at a concentration of 1,000 ppm by 28.60 ± 1.10%, which is categorized as very weak relative to the results from acarbose. Further investigation is necessary to optimize the bioactive compounds and identify the content of anti-nutritional compounds, as well as to explore effective methods of processing local Timor velvet beans that can reduce the antinutritional compounds.
Pengaruh Model Problem Based Learning terhadap Kemampuan Generik Sains dan Pemahaman Konsep Siswa Hambarwati, Sri; Muhali, Muhali; Taloin, Rensani
Multi Discere Journal Vol. 3 No. 2 (2024): November
Publisher : LITPAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/mj.v3i2.2236

Abstract

Penelitian ini bertujuan untuk mengkaji pengaruh model Problem-Based Learning (PBL) terhadap kemampuan generik sains dan pemahaman konsep siswa pada materi reaksi reduksi-oksidasi. Metode kuasi-eksperimen dengan desain pretest-posttest control group digunakan pada 53 siswa kelas X di SMA Islam Al-Azhar NW Kayangan, yang dibagi secara acak menjadi kelompok eksperimen (n=25) dan kontrol (n=28). Hasil penelitian menunjukkan bahwa kelompok eksperimen yang diajarkan dengan PBL mengalami peningkatan signifikan dalam kemampuan generik sains, terutama pada indikator bahasa simbolik (nilai rata-rata posttest 75,00) dan inferensi logika (29,00), dengan signifikansi 0,000. Peningkatan pemahaman konsep siswa juga signifikan pada kelompok eksperimen, dengan rata-rata posttest 70,60 dibandingkan kelompok kontrol yang hanya mencapai 46,00 (signifikansi 0,003). Temuan ini menunjukkan bahwa PBL lebih efektif daripada metode konvensional dalam meningkatkan kemampuan generik sains dan pemahaman konsep siswa dalam materi kimia yang kompleks. Penerapan PBL dalam pembelajaran kimia di sekolah disarankan untuk meningkatkan kualitas pemahaman siswa terhadap konsep-konsep abstrak. The Effect of Problem-Based Learning Model on Students’ Generic Science Skills and Conceptual Understanding  Abstract This study aimed to examine the effect of the Problem-Based Learning (PBL) model on students' generic science skills and conceptual understanding of redox reactions. A quasi-experimental method with a pretest-posttest control group design was employed with 53 tenth-grade students from SMA Islam Al-Azhar NW Kayangan, who were randomly assigned into an experimental group (n=25) and a control group (n=28). The findings revealed that the experimental group taught using PBL showed a significant increase in generic science skills, particularly in symbolic language (posttest mean score of 75.00) and logical inference (29.00), with a significance level of 0.000. Conceptual understanding also improved significantly in the experimental group, with a posttest mean score of 70.60, compared to 46.00 in the control group (significance level of 0.003). These results indicate that PBL is more effective than conventional methods in enhancing both generic science skills and conceptual understanding in complex chemistry topics. The study recommends PBL application in chemistry education to deepen students' understanding of abstract concepts.