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All Journal HAYATI Journal of Biosciences Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
Darah Ibrahim
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Engineering Other

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Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64 Mat Jalil, Mohd Taufiq; Zakaria, Nurul Aili; Yahya, Mohd Fakharul Zaman Raja; Mohamad, Sharifah Aminah Syed; Ibrahim, Darah
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.445-458

Abstract

The biological activities of ethyl acetate extracts of Lasiodiplodia pseudotheobromae IBRL OS-64 were assessed in the present study. Antioxidant activity was measured using the DPPH scavenging assay, antibacterial activity via broth microdilution, and total phenolic content using the Folin-Ciocalteu method. In vivo toxicity was determined using a brine shrimp lethality assay. The fraction extract exhibited the highest antioxidant activity, with an EC50 of 441.6 µg/ml, surpassing the crude extract's. The EEELP showed a relatively low phenolic content of 13.273 µg GAE/mg extract. Regarding antibacterial activity, both crude and fraction extracts demonstrated MIC values ranging from 62.5 to 250 µg/ml against Gram-positive bacteria, while MBC values ranged from 125 to 500 µg/ml for both extracts. The MIC values for crude and fraction extracts against Gram-negative bacteria were 250-500 µg/ml, whilst the MBC values for both crude and fraction extracts were in the range of 500–2,000 µg/ml. The findings also revealed that Gram-positive bacteria were more susceptible to both extracts (crude and fraction) than Gram-negative bacteria. The crude extract exhibited a non-cytotoxic effect with an LC50 value of 2054.88 µg/ml for acute exposure and a low cytotoxic effect with an LC50 value of 199.69 µg/ml for chronic exposure. Meanwhile, the fraction extract demonstrated a non-cytotoxic effect for both acute and chronic with an LC50 value of 5744.14 µg/ml and 1035.94 µg/ml, respectively. Therefore, the present study suggests that the fraction extract could be an effective pharmaceutical agent and safe for drug development due to its low toxicity.
Co-Authors Akihiko Kosugi Akihiko Kosugi Amir Modarresi Chahardehi Anuradha Balan Farid Abolhassani I Nyoman Pugeg Aryantha I Nyoman Pugeg Aryantha Ibrahim Che Omar LATIFFAH ZAKARIA Lee Kok Chang Lim Sheh Hong Lim Sheh Hong Mat Jalil, Mohd Taufiq Mohamad, Sharifah Aminah Syed Panida Prawitwong Rashidah Abdul Rahim Shaida Fariza Sulaiman Syarifah A. Rashid Syarifah A. Rashid Takamitsu Arai Takamitsu Arai Tong Woei Yenn Tong Woei Yenn Yahya, Mohd Fakharul Zaman Raja Yutaka Mori Zakaria, Nurul Aili