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Idwan Sudirman
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Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering Veterinary

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echanism at Antibacterial Activity of Lb. plantarum kik Metabolites and Monoacylglycerol Coconut Oil upon Pathogenic Bacteria Asriani .; Betty Sri Laksmi; Sedernawati .; Idwan Sudirman
Jurnal Teknologi dan Industri Pangan Vol. 18 No. 2 (2007): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Antibacterial mechanism of mixture between metabolites Lb.plantarum klik and monoacylglycerol coconut oil was found through analysis of the MIC levels. The level of 1 and 2 MIC can increase the leakages of the gram positif bacterial sell (L.monocytogenes and B.cereus) and that of the gram negative bacteria (S.typhimurium). The leackages of cell was measured by spectrofotometer and represented increasing of the absorbance of the protein nucleic acid . The absorbance of metal ion was evaluated using a AASS (measured by Atomic Absorption Spectrophotometer) and it indicated that the absorbance increased of 40.2% and 22.1% for Ca 2+ and K+ respectively. Observation of cell damage on L. monoctogenes and S. tyhimurium using SEM (scanning Electron Microscopy) resulted in morphological damage on both MIC 1 and 2 in which MIC 2 was severly damage. Key word : antibacterial mechanism, monoacylglycerol coconut oil, minimum inhibitory concentration
Pemanfaatan Supernatan Lactobacillus plantarum Sebagai Penghambat Pertumbuhan Escherichia coli pada Dangke Susu Sapi (UTILIZATION OF LACTOBACILLUS PLANTARUM SUPERNATAN AS AN INHIBITIOR OF ECHERICHIA COLI GROWTH IN COW’S MILK DANGKE Nining Arini; Mirnawati Sudarwanto; Idwan Sudirman; Agustin Indrawati
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Dangke is a traditional food in Enrekang, a district in South Sulawesi. Its made from buffalo’s milk orcow’s milk. Dangke could be contaminated during the process with Escherichia coli which causes diarrheain children and adults. It was known that supernatant of Lactobacillus plantarum has antibacterial capacity,and it may be used as a biopreservative agent. The research aims were to determine the minimum inhibitoryconcentration (MIC) of L. plantarum supernatant in inhibiting the growth of E. coli ATCC 25922, determinethe nutrients level of cow’s milk dangke after the addition of 1% and 2% milk fat, as well as determine theeffect of L. plantarum supernatant and cow’s milk fat addition into dangke inhibited the growths of pathogenicbacteria of E. coli ATCC 25922. MIC value was determined based on the value of the lowest concentrationof supernatant that shown with no any bacteria growth in the media. Data of pathogenic bacteria growth analyzed with analysis of variance test with a 2x2 factorial design, which 1st factor was the addition of L.plantarum supernatant (with or without addition of supernatant) and the second factor was the additionof fat content (1% and 2%) and time observation was made on days 0, 2nd, 4th, 6th, and 8th. Resultsshowed that the filtrate of fermented L. plantarum was able to inhibit the growth of E. coli ATCC 25922in vitro and had 10% minimum inhibitory concentration (MIC). Level of fat and protein in dangke whichadded 1% cow’s milk fat, was higher than with 2% cow’s milk fat. The L. plantarum supernatant is provedto be able to inhibit the growth of E. coli ATCC 25922. Therefore, it is potentially used as a naturalbiopreservative agent in making dangke.
Praktek Sanitasi Higiene pada Usaha Pengolahan Dangke Susu Sapi di Kabupaten Enrekang, Sulawesi Selatan (THE PRACTICES OF HYGIENIC SANITATION IN PROCESSING INDUSTRIES OF COW MILK DANGKE IN ENREKANG DISTRICT, SOUTH SULAWESI) Wahniyathi Hatta; Mirnawati Bachrum Sudarwanto; Idwan Sudirman; Ratmawati Malaka
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Lack of hygienic sanitation conditions in food processing industry could contribute to the food safety.The aim of this study was to assess the implementation of hygienic sanitation practices in the processingindustries of cow milk dangke in Enrekang District, South Sulawesi. Total of 60 respondents wereinterviewed using questionnaire and observation on hygienic sanitation practices was carried out usingcheck-list.  Respondents were producers and also workers in the processing units in Cendana Sub-district,Enrekang which had been selected with simple random sampling technique.  Scoring of hygienic sanitationcondition were based on the application of hygienic sanitation practices on the checklist.  None of workerswore special clothes, put dangke molds in closed container, and wiped packaging of dangke with specialclothes.  Less than 50% of the workers  wore a head coverings, prepared papaya latex with boiled water,and covered dangke when molding.  A total of 50% of the workers immediately washed the molds afterused.  More than 50% of the workers washed their hands with soap before dangke processing, washed themolds with soap, wiped the packagings with a cloth before it used, stored the milk in a closed container,and cleaned papaya fruit before preparing papaya latex.  All of the workers made dangke while doinghousework. Hygienic sanitation condition of dangke processing industries that had good category was 3%while another 57% in moderate category and poor category was 40%.  The hygienic sanitation practices hadnot been fully implemented on the processing industries of cow milk dangke in Enrekang district, which ledto the category of hygienic sanitation condition of largely of them was moderate .
Co-Authors Agustin Indrawati Asriani . Betty Sri Laksmi Mirnawati Bachrum Sudarwanto Mirnawati Sudarwanto Nining Arini Ratmawati Malaka Sedernawati . Wahniyathi Hatta