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EFEKTIVITAS ENKAPSULASI ENZIM PROTEASE DENGAN BEAD HIDROGEL BERBAHAN ALGINAT-KITOSAN Christian Joseph Tanuwidjaja; Eko Adi Prasetyanto
Bahasa Indonesia Vol 21 No 1 (2022): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v21i1.1944

Abstract

Introduction: The addition of the protease enzyme from an external source can increase protein absorption in the human digestive system. Protease enzyme is formulated by encapsulated inside alginate-chitosan hydrogel beads to deny immediate proteolysis reaction with the protein consumed and maintain the encapsulated enzyme in a temperature range acidity. This research aims to know the encapsulation effectivity of protease enzyme with alginate-chitosan hydrogel beads. Methods: Experiments were conducted to encapsulate protease enzyme with ionotropic gelation method using alginate and chitosan. Encapsulation yield, bead size, encapsulated enzyme activity, release rate, and stability were analyzed from the hydrogel beads that were obtained. The data is then analyzed using analysis of variance (ANOVA) and Duncan’s Multiple Range Test (DMRT). Results: From four variants of hydrogel beads that were obtained, this research shows that the volume addition of alginate solution in the encapsulation material will increase the value of encapsulation yield (EY), bead size, and release rate of the encapsulated protease enzyme. Therefore, the volume addition of chitosan solution will increase the value of encapsulation efficiency (EE). Hydrogel beads incubation at 6°C for 24 days maintain 75,55% of encapsulated enzyme activity. Conclusion: Protease enzyme can be formulated with protein by being encapsulated with alginate-chitosan hydrogel beads. Key Words: alginate, chitosan, protease enzyme encapsulation
Pengembangan Molecular Imprinted Polymer Untuk Pemisahan Vitamin C dalam Sediaan Multivitamin Untung Gunawan; Eko Adi Prasetyanto; Shannen Ivanka Gunardi
Jurnal Farmasi Indonesia Vol 18 No 1 (2021): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/jfi.v18i1.1155

Abstract

Vitamin C has chromophore group that can be analyzed using UV-Vis spectrophotometry, however if the complex matrix to be analyzed like multivitamins, a selective separation method is required to eliminate matrix interferences. One of the selective separation methods is Molecular Imprinted Polymer (MIP) which which hasn’t been widely used in multivitamin samples. The purpose of this study was to obtain selective MIP for separation of vitamin C in multivitamins.. Research methods included interaction observation of vitamin C with functional monomers using UV spectrophotometry, MIP was synthesized using bulk method for 24 hours at 60 °C. MIP was characterized using spectrophotometry, infrared and scanning electron microscopy MIP selectivity were determined by determination of imprinting factor and its recovery with the liquid-liquid extraction method (LLE) and SPE C18 for separation of vitamin C in multivitamin samples on the market using a validated method. Hypsochromic shift showed interaction between vitamin C and functional monomers, which hydrogen bonds were taking role in MIP synthesis. Imprinting factor more than 1 and recovery value close to 100% indicated MIP selectivity to vitamin C. MIP-1 had the highest selectivity compared to the ECC and SPE C18 methods for separation of vitamin C in multivitamin samples with recovery of 98.17 ± 2.31%, 69.42 ± 3.01%, and 93.26±4.24% respectively. Synthesized MIP-1 was selective for the separation of vitamin C in multivitamins.
GATE DELIVERYBOT SEBAGAI SOLUSI DI MASA PANDEMI UNTUK UMKM KULINER BEATYOURMEAT.ID Filian Arbiyani; Eko Adi Prasetyanto; Juan Almer Dynan; Andreas Halim; Alvin Henrijento; Anugrah Jordan Signori; John Louis Christopher Tanaya; Alexander Boentoro
Jurnal Bakti Masyarakat Indonesia Vol 4, No 3 (2021): Jurnal Bakti Masyarakat Indonesia
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jbmi.v4i3.12855

Abstract

During the COVID-19 pandemic, many activities have to be done from home, including buying foods through online delivery. Beatyourmeat.id is one of the culinary micro enterprises (SME) located in Bogor who receives many orders through online platform during COVID-19 pandemic. The food from Beatyourmeat.id’s kitchen is delivered manually by the Beatyourmeat.id’s employees to online drivers or couriers. This manual process is vulnerable to COVID-19 spreading. Therefore, a solution to replace this manual process is highly needed. The advance solution is a food delivery robot that is built through this community service program, named GATE DELIVERYBOT. GATE DELIVERYBOT is equipped with an eminence feature, i.e. an automatic hand sanitizer, to clean the couriers’ hands before grabbing the food inside the box. A visit to Beatyourmeat.id location is done as a starting step in this community service program. Following the Robot design process according to Beatyourmeat.id’s layout, component purchasing, assembling, coding, then testing, implementation and evaluation process. GATE DELIVERYBOT has successfully operated in delivering the packages or foods from the kitchen to online couriers by following the certain path. An automatic hand sanitizer feature is also successfully operated by dispensing hand sanitizer liquid while a hand detected as an input in the sensor. Satisfaction comes from Beatyourmeat.id, a micro enterprise (SME) served in this program, when GATE DELIVERYBOT implemented. Beatyourmeat.id gives score 4 (the best score) and provides feedback forfuture GATE DELIVERYBOT developmentABSTRAK:Di masa Pandemi COVID-19, banyak aktivitas yang harus dilakukan dari rumah, salah satunya adalah proses pembelian makanan yang harus dilakukan secara daring dari rumah. Beatyourmeat.id adalah salah satu bisnis usaha mikro di daerah Bogor yang bergerak di bidang kuliner dan banyak menerima pesanan secara daring selama masa Pandemi COVID-19. Proses pengantaran makanan dari area dapur Beatyourmeat.id kepada kurir atau supir ojek daring selama ini masih dilakukan secara manual oleh karyawan Beatyourmeat.id sehingga rentan terjadi persebaran virus Corona. Oleh karena itu, dibutuhkan solusi berupa robot pengantar makanan yang dapat menggantikan proses pengantaran manual ini. Robot pengantar makanan yang dikembangkan dalam program Pengabdian kepada Masyarakat ini dinamakan GATE DELIVERYBOT yang dilengkapi dengan kelebihan fitur sistem auto hand sanitizer dispenser atau hand sanitizer otomatis untuk membersihkan tangan kurir atau supir ojek daring sebelum mengambil makanan yang terdapat di dalam boks. Proses pelaksanaan program Pengabdian kepada Masyarakat ini diawali dengan survei lokasi mitra, lalu pembuatan desain Robot menyesuaikan tata letak lokasi mitra, yang dilanjutkan dengan proses pembelian alat dan komponen, lalu perakitannya, yang kemudian dilanjutkan dengan proses pemrograman robot, sampai pada tahap pengujian, implementasi dan evaluasi. GATE DELIVERYBOT telah berhasil berjalan dan mengantarkan paket atau makanan dengan lancar sesuai jalur yang telah ditentukan. Fitur auto hand sanitizer dispenser juga telah berhasil beroperasi mengeluarkan cairan hand sanitizer ketika ada input tangan pengguna. Mitra Beatyourmeat.id sangat puas dengan memberikan nilai 4 (terbaik) pada semua aspek penilaian dan memberikan saran pengembangan agar ke depannya GATE DELIVERYBOT dapat dikendalikan melalui handphone.
Use of molecular imprinted polymer for isolation of secondary metabolites in plants for drug raw material independence Gunawan, Untung; Prasetyanto, Eko Adi; Falena, Pretty; Irlianto, Kellie; Ivansyah, Atthar Luqman
Science Midwifery Vol 13 No 4 (2025): Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v13i4.2077

Abstract

Indonesia, with its rich biodiversity, has numerous plant species with significant therapeutic potential. Secondary metabolites in plants play a vital role in pharmaceuticals, agriculture, and the food industry. However, traditional methods for isolating these compounds are often non-selective, time-consuming, and environmentally unfriendly. Molecularly Imprinted Polymer (MIP) is an emerging method for isolating secondary metabolites due to its selectivity and efficiency. MIP has been successfully applied in the identification and separation of plant-derived compounds, including secondary metabolites, chemical residues, and pesticides. This study explores the use of MIP for isolating secondary metabolites in plants and aims to contribute to developing strategies for obtaining targeted metabolites. The findings also highlight future research opportunities for MIP in industrial applications to support the independence of medicinal raw materials in Indonesia.