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An Analyses The Growth And Adaptation Of Mangrove Seedlings Of Rhizophoraceae Family On Different Level Of Seawater Inundation To Support Mangrove Ecosystem Restoration Planning Analuddin Analuddin; Andi Septiana; Jamili Jamili; Asrun Budiatma; Musdalifa Musdalifa; wiwin Wiwin; Sahidin Sahidin
Jurnal Perencanaan Wilayah Vol 4, No 2 (2019)
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.186 KB) | DOI: 10.33772/jpw.v4i2.9975

Abstract

The purposes of this study were to know the impact of different level of sea water inundation on the growth capacity and biochemical adaptation of the mangrove Rhizophoraceae family of Rhizophora mucronata and Bruguiera gymnorrhiza seedlings. The propagules of these mangroves were planted with different levels of sea water flooding, namely 90 cm, 60 cm, 45 cm and 30 cm. The growth of height and diameter of these mangrove seedlings were measured at early and termination of treatments, while their biochemical adaptations were analyzed in the Laboratory. The relatively growth rate RGR of high and diameter of these seedlings were analyzed, while statistical test was performed by anova. The RGR of high of the two mangrove seedlings were significantly different (P <0.05), though their RGR of diameter did not differ significantly (P> 0.05). The growth of height of R. mucronata seedlings was fastest at the lower level of inundation (30 cm) and the slowest at the higher level of submergence (90 cm). On the contrary, the height growth of B. gymnorrhiza seedlings was fastest at submergence of 90 cm, and the slowest at submergence of 30 cm. The flavonoids and vitamin C contents in both mangrove seedlings increased significantly (P <0.05) with increased inundation levels. Thus, although biochemical responses tended to be the same for both mangroves, but B. gymnorrhiza seedlings indicate  more able to grow and develop on rising sea levels than R. mucronata seedlings. This study provides important information for revegetation planning of degraded mangrove under a sea level rise scenario.Keywords: Biochemical adaptation, Sea water level, mangrove seedling growth, mangrove revegetation.
Spermatogenesis Mencit (Mus musculus, L.) Pasca Pemberian Ekstrak Brotowali (Tinospora crispa, L.) Wa Ode Harlis; Andi Septiana; Arjuni Arjuni
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 1 (2019): Biology and Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.388 KB) | DOI: 10.33772/biowallacea.v6i1.8746

Abstract

ABSTRAKBrotowali (Tinospora crispa, L.) merupakan tumbuhan  yang mengandung senyawa yang tergolong dalam kelompok antifertilitas diantaranya golongan glikosida, alkaloid, flavonoid,  saponin dan tanin. Kelompok senyawa tersebut dapat memberikan efek sitotoksik pada reproduksi jantan dengan mengganggu metabolisme sel germinal dan sel spermatogenik. Tujuan penelitian ini untuk mengetahui spermatogenesis mencit pasca diberikan ekstrak  brotowali. Sebanyak 20 ekor mencit jantan berat 30-40 gr, berumur 2-3 bulan dibagi 5 kelompok perlakuan  yaitu; K1 kontrol positif  (aquades), K2 kontrol negatif (Na CMC 0,5% ), K3 (0,05 g/g bb), K4 (0,06 g/g bb), dan K5 (0,07 g/g bb) ekstrak brotowali. Ekstrak diberikan secara oral setiap hari selama 34 hari. Pada hari ke-35 mencit dikorbankan dengan kloroform selanjutnya pengambilan organ testis untuk dibuat preparat mikroanatomi. Data dianalisis dengan ANOVA dan uji BNT (α=0,05%). Hasil penelitian menunjukkan bahwa pemberian ekstrak tumbuhan brotowali secara signifikan mengganggu proses spermatogenesis mencit dengan menurunkan jumlah sel spermatogonium, spermatosit primer, sel spermatid dan kepadatan spermatozoa. Penurunan jumlah sel-sel spermatogenik dalam penelitian ini terbanyak pada perlakuan K5 untuk semua kelompok, dengan jumlah spermatogonium (28,5), dengan jumlah spermatosit primer (33,3), dengan jumlah spermatid (69,4) dan kepadatan spermatozoa (1,19).                                                                                 Kata kunci : Ekstrak, Tinospora crispa, L., Spermatogenesis, Mencit ABSTRACTBrotowali (Tinospora crispa, L.) is a plant that contains compounds belonging to the antifertility group including glycosides, alkaloids, flavonoids, saponins and tannins. This group of compounds can provide cytotoxic effects on male reproduction by disrupting germinal cell metabolism and spermatogenic cellsThe aim of this study was to know the effect of extracts Tinospora crispa, L. on spermatogenesis of mice (Mus musculus, L.). The male mice were used 20 with weight 30-40 g, aged 2-3 months, and were treated in 5 groups i.e. K1 : positive control (aquadest), K2 : negative control (Na CMC 0.5%), K3 (0.05 g/g bb), K4 : (0.06 g/g bb), and K5 (0.07 g/g bb) extracts of brotowali. The extracts were given orally during 34day. At the 35th the weight of mice werw counted and then sacrificed with chloroform, and the testis were collected to make microanatomy. The data were analyzed with ANOVA (Analysis of Varience) and t-Test (α = 0,05%). The results showed that the treatments with brotowali extracts obstructed spermatogenesis process and reduced the amount of spermatogonium cells, primary spermatocyt, spermatide cells and density of spermatozoa with increasing dosage of brotowali extracts. The decreasing of spermatogenic cells were observed in dosage of K5 with spermatogonium cells (28,5), the amount primary spermatocyte (33,3), the amount of spermatide (69,4) and the density of spermatozoa (1,19).Key word : Extracts, Tinospora crispa, L., Spermatogenesis, Mice
GAMBARAN HISTOLOGI TESTIS MENCIT (Mus musculus, L.) SETELAH PEMBERIAN EKSTRAK TUMBUHAN BROTOWALI (Tinospora crispa, L.) Wa Ode Harlis; Andi Septiana
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 4, No 1 (2017): Biosains & Technology in Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.378 KB) | DOI: 10.33772/biowallacea.v4i1.3276

Abstract

ABSTRAK Tumbuhan Brotowali (Tinospora crispa, L.) diketahui mengandung senyawa yang tergolong dalam kelompok antifertilitas diantaranya golongan glikosida, alkaloid, flavonoid, saponin dan tanin. Kelompok senyawa-senyawa tersebut dapat memberikan efek sitotoksik pada reproduksi jantan dengan mengganggu metabolisme sel germinal dan sel spermatogenik. Tujuan penelitian ini untuk mengetahui pengaruh ekstrak brotowali terhadap spermatogenesis mencit. 20 ekor mencit jantan berat 20-30 gr, berumur 2-3 bulan dibagi 5 kelompok yaitu; K1 kontrol negatif  (aquades), K2 kontrol positif (Na CMC 0,5% ), K3 (0,05 g/g bb), K4 (0,06 g/g bb), dan K5 (0,07 g/g bb) ekstrak brotowali. Ekstrak diberikan secara oral selama 34 hari. Pada hari ke-35 dikorbankan dan dilakukan pengambilan organ testis untuk preparat mikroanatomi. Penelitian bersifat deskriptif, data disajikan dalam bentuk gambar. Berdasarkan hasil penelitian menunjukkan bahwa pada penampang melintang testis nampak jelas terjadi penurunan  jumlah sel sel spermatogenik  dalam tiap penampang tubulus seminiferous mencit, pada semua perlakuan yang diberikan ekstrak tumbuhan brotowali seiring dengan meningkatnya dosis yang diberikan Kata Kunci : Tinospora crispa, L., histologi  testis, Mencit ABSTRACTThe Brotowali plant (Tinospora crispa, L.) is known to contain compounds belonging to the antifertility group such as glycosides, alkaloids, flavonoids, saponins and tannins.These groups of compounds may have a cytotoxic effect on male reproduction by disrupting the metabolism of germ cells and spermatogenic cellsThe aim of this study was to knowthe effect of extracts Tinospora crispa, L. on spermatogenesis of mice. The male mice were used 20 weight 30-40 g, aged 2-3 months, and were treated in 5 groups i.e. K1 : negative control (aquadest),K2 : positive control (Na CMC 0.5%), K3 (0.05 g/g bb), K4 : (0.06 g/g bb), and K5 (0.07 g/g bb) extracts of brotowali. The extracts were given orally during 34 day.At the 35 th the testis were collected to make microanatomy. Research is descriptive, data is presented in the form of picture. Based on the results showed that crosssectional testis clearly visible decrease in the number of spermatogenic cell cells in each section of tubular seminiferous mice, in all treatments given extracts of brotowali plants along with increased doses given.
Innovation in Scad Mackerel (Decapterus spp.) Processing Into Economically Valuable Products for Improving The Skills of Coastal Communities in Nambo Village Sitti Wirdhana Ahmad; Nur Arfa Yanti; Muhsin Muhsin; Andi Septiana; Muzuni Muzuni; Taufik Walhidayah
ABDIMAS: Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2022): ABDIMAS UMTAS: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Muhammadiyah Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.919 KB) | DOI: 10.35568/abdimas.v5i1.1668

Abstract

Scad Mackerel (Decapterus spp.) is the main consumption commodity of coastal communities in Nambo village. Apart from public awareness is quite high, the price is affordable, and has nutritional value since unsaturated fats is low, so it good to consume for all ages. The public lack of knowledge about proper fish management. The solution is to provide an understanding of fish processing techniques so be more selling, delicious and nutritious, as well as for community empowerment with the aim of creating fisheries entrepreneurs. The method was the lecture method, socialization and training to the community as an empowerment effort to improve knowledge, skills and well-being. The implementation of the service in the form of location surveys, coordination with local regional chief of village and PKK administrators, preparation of equipment and materials, and some processed samples of fish, socialization about the benefits of fish and fish processing training