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KEBUNTINGAN MENCIT (Mus Musculus L.) TAHAP PASCA IMPLANTASI LANJUT. PASCA PEMBERIAN EKSTRAK RIMPANG JAHE PUTIH (Zingiber Officinale Var. Amarum) PREGNANCY MICE (Mus Musculus L.) POST-IMPLANTATION STEP FURTHER WITH POST GIVE WHITE GINGER RHIZOME EXTRACT Wa Ode Harlis
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 4, No 2 (2017): Biodiversity Wallacea
Publisher : University of Halu Oleo

ABSTRAKKandungan senyawa metabolit sekunder yang terdapat pada tanaman jahe (Zingiber officinale var. Amarum) diantaranya golongan flavonoid, fenolik, terpenoid dan minyak atsiri. Golongan terpenoid dan minyak atsiri yang terkandung pada rimpang jahe putih dapat bersifat sebagai antifertilitas pada hewan jantan yaitu dapat mencegah proses transportasi sperma, sedangkan minyak atsiri dapat menggumpalkan sperma sehingga menurunkan motilitas dan daya hidup sperma yang menyebabkan sel sperma tidak mencapai sel telur dan pembuahan dapat tercegah. Tujuan dari penelitian ini untuk mengetahui pengaruh ekstrak rimpang jahe putih terhadap kebutingan mencit (Mus musculus L.) tahap pasca-implantasi lanjut. Jenis penelitian ini menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL) dianalisis dengan Analysis of Variance (ANOVA, α = 0,05) yang terdiri dari 4 perlakuan dan 4 ulangan. Sebanyak 16 ekor mencit betina dengan berat 20-30 gr berumur 2-3 bulan dibagi ke dalam 4 kelompok yaitu K1 (Aquades), K2 (0,7mg/g bb), K3 (1,4 mg/gbb), dan K4 (2,8 mg/gbb) ekstrak. Ekstrak diberikan secara oral sebanyak 0,5 mL/ekor/hari, pada kebuntingan hari ke-8 sampai ke-12. Mencit dipelihara sampai hari kebuntingan ke-18. Mencit dikorbankan dengan menggunakan klorofom dan dibedah. Selanjutnya dilakukan pengamatan persentase fetus hidup dan persentase fetus mati. Hasil penelitian menunjukkan bahwa ekstrak rimpang jahe putih memiliki pengaruh terhadap kebuntingan mencit tahap pasca implantasi lanjut dengan menurunkan persentase fetus hidup yaitu K1 (1,01%), K2 (0,63%) dan K3 (0,4%), sedangkan K4 tidak ditemukan fetus. Meningkatkan persentase fetus mati pada perlakuan K3 (1,4 mg/g) yaitu 0,4%. Kata Kunci : Ekstrak Rimpang Jahe Putih, Kebuntingan Pasca Implantasi Lanjut. ABSTRACT The content of secondary metabolite compounds found in ginger plants (Zingiber officinale var. Amarum) including flavonoids, phenolic, terpenoids and essential oils. Terpenoids and essential oils contained in white ginger rhizome can be as antifertility in male animals that can prevent sperm transport process, while the essential oil can agglomerate sperm to reduce the mortality and sperm life that causes sperm cells do not reach the egg and fertilization can be prevented. The aim of this research was to know the effect of white ginger rhizome extract (Zingiber officinale var. Amarum) on the post-implantation advanced stage of mice (Mus musculus L.). This research was experimental method with completely randomized design (CRD) which consists of 4 treatments and 4 replications. The data was analyzed by Analysis of Variance (ANOVA, α = 0,05). A total of 16 female mice weighing 20-30 g 2-3 months old were divided into 4 groups : K1 (Aquades), K2 (0.7 mg/gb), K3 (1.4 mg/gbb), and K4 ( 2.8 mg/gb) extract of white ginger rhizome. The extract was administered orally as 0.5 ml/ head/day, on gestation day 8 to 12. The mice were maintained up to 18 days gestation. Mice were sacrificed by using chloroform and dissected. Further more, the observation of the parameters of post-implantation gestation advanced stage is the percentage of fetuses and fetal death. The results showed that the extracts of white ginger rhizome has an influence on the post-implantation stage of gestation mice by reduce the percentage of live fetuses are K1 (1.01%), K2 (0.63%) and K3 (0.4%), while the K4 not found fetus. Increase the percentage of dead fetus on treatment of K3 (1.4mg/g) is 0.4%. Keywords : The Extract of White Ginger Rhizome Extract, The Post Implantation Advanced Stag

Jumlah Sel - Sel Spermatogenik Testis Mencit (Mus musculus L.) Setelah Pemberian Ekstrak Mangrove Bruguiera gymnorrhiza Lamk. Wa Ode Harlis; Jamili Jamili; Ernawati Ernawati
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 2 (2021): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Tujuan penelitian ini untuk mengatahui pengaruh ekstrak mangrove Bruguiera gymnorrhiza Lamk. terhadap komposisi sel spermatogenik testis mencit (Mus musculus L.). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), terdiri atas 4 perlakuan dengan masing-masing enam ulangan, yaitu kontrol (Na CMC 0,5%), dan pemberian ekstrak daun, kulit batang dan buah mangrove Bruguiera gymnorrhiza Lamk. dosis 20mg/kg BB. Ekstrak diberikan secara oral selama 34 hari. Sehari setelah perlakuan berakhir, organ testis diisolasi dan dibuat sediaan preparat histologis. Parameter yang diamati yaitu jumlah sel-sel spermatogonia, sel spermatosit primer, sel spermatid dan kepadatan spermatozoa mencit. Metode perhitungan dilakukan secara langsung dengan mengamati sel-sel yang tersusun di tubulus seminiferus pada sediaan histologi testis untuk setiap perlakuan dan ulangan dengan menggunakan kamera mikroskopik dan software Image Raster dari Optilab. Data yang diperoleh dianalis dengan One Way Anova dan uji lanjut BNT (α=0,05) untuk perlakuan yang menunjukkan perbedaan nyata. Pemberian ekstrak daun, kulit batang dan buah mangrove Bruguiera gymnorrhiza (L) Lamk secara signifikan menurunkan jumlah sel sel spermatogenik mencit (Mus musculus L.) dengan susunan sel sel spermatogenik yang semakin tidak teratur, renggang dan lumen yang tidak terisi penuh spermatozoa. Penurunan jumlah sel-sel spermatogenik dalam penelitian ini terbanyak pada perlakuan ekstrak daun.Kata Kunci: Bruguiera gymnorrhiza Lamk, Spermatogonia, spermatosit primer, spermatid. Kepadatan spermatozoa, mencit jantan ABSTRACTThe purpose of this study was to determine the effect of the mangrove extract of Bruguiera gymnorrhiza Lamk. on the spermatogenic cell composition testicular of mice (Mus musculus L.). This study used a completely randomized design (CRD), consisting of 4 treatments with six replications each, namely control (Na CMC 0.5%), and administration of Bruguiera gymnorrhiza Lamk leaf, bark and mangrove fruit extracts. dose of 20mg / kg BW. The extract was administered orally for 34 days. The day after the treatment ended, the testicular organs were isolated and histological preparations were made. The parameters observed were the number of spermatogonia cells, primary spermatocytes, spermatid cells and the density of spermatozoa in mice. The calculation method was carried out directly by observing the cells arranged in the seminiferous tubules on the testicular histology for each treatment and replication using a microscopic camera and Image Raster software from Optilab. The data obtained were analyzed with One Way Anova and LSD further test (α = 0.05) for treatments that showed significant differences. The administration of leaf extract, stem bark and mangrove fruit Bruguiera gymnorrhiza (L) Lamk significantly reduced the number of spermatogenic cells of mice (Mus musculus L.) with increasingly irregular, loosened and lumen cells that were not fully filled with spermatozoa. The decrease in the number of spermatogenic cells in this study was mostly in the leaf extract treatment.Key words: Bruguiera gymnorrhiza (L) Lamk, spermatogonia, primary spermatocytes, spermatids, male mice

Pengaruh Salep Ekstrak Daun Nilam (Pogestemon cablin Benth.) Pada Penyembuhan Luka Bakar Mencit (Mus musculus L.) Wa Ode Harlis; Nurhayu Malik; Dwi Arinto Adi; Inda Yani
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 7, No 2 (2020): Biodiversitas on Asian Wallacea
Publisher : University of Halu Oleo

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The purpose of this study was to determine the influence of patchouli leaf extract ointment (Pogestemon cablin Benth.) To cure burns of mice (Mus musculus L.) A total of 25 mice aged 2-3 months, divided into 5 treatment groups and 5 replications namely K- control (air), K+ (bioplacenton), K1 (patchouli leaf extract ointment 10 mg/g), K2 (patchouli leaf extract ointment 15 mg/g) and K3 (patchouli leaf extract ointment 20 mg/g). Burns are made by means of the backs of mice induced by hot metal coins with an area of 1 cm and a depth of 0.2 cm. Patchouli leaf extract ointment was given after making the wound in all groups by administering extract 13.760 mg/head/day for K1 20.25 mg/head/day and 27.5 mg/ head/day with one time daily administration for 14 days. The parameters observed in the healing of burns are the absence of exudate (blistered skin), erythema (redness of the skin), swelling, the length of time the wound closes and the wound heals which is marked by a completely closed wound and the growth of hair on the back of mice. This study was an experimental study using a Completely Randomized Design (CRD), data on the duration of wound healing were analyzed by ANOVA (α = 0.05%) and LSD test. The results showed that the dosage of patchouli leaf extract (Pogestemon cablin Benth.) K3 20 mg/g is more effective in accelerating the healing time of burns compared to a positive control (bioplacenton 10%) or administration of synthetic drugs.Abstrak Penelitian ini bertujuan untuk mengetahui pengaruh salep ekstrak daun nilam (Pogestemon cablin Benth.) pada penyembuhan luka bakar mencit (Mus musculus L.) 25 ekor mencit yang berumur 2-3 bulan, dibagi dalam 5 kelompok perlakuan dan 5 ulangan yaitu K- kontrol (air), K+ (bioplacenton10 %), K1 (salep ekstrak daun nilam 10 mg), K2 (salep ekstrak daun nilam 15 mg) dan K3 (salep ekstrak daun nilam 20 mg). Pembuatan luka bakar dilakukan dengan cara punggung mencit diinduksi dengan koin logam panas dengan luas 1 cm dan kedalaman 0,2 cm. Pemberian salep ekstrak daun nilam diberikan setelah pembuatan luka pada semua kelompok dengan pemberian salep ekstrak 13,95 mg/ekor/hari untuk K1 20,55mg/ekor/hari dan 27,9 mg/ekor/hari dengan waktu pemberian satu kali sehari selama 14 hari. Parameter pengamatan peyembuhan luka bakar yaitu tidak adanya kulit melepuh, eritrema (kemerahan pada kulit), pembengkakan, lama waktu luka menutup dan luka sembuh yang ditandai dengan luka menutup sempurna dan tumbuhnya bulu pada punggung mencit.Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL), data lama waktu peyembuhan luka dianalisis dengan ANOVA (α=0,05%) dan uji BNT Hasil Penelitian menunjukkan bahwa dosis salep ekstrak daun nilam (Pogestemon cablin Benth.)K3 20 mg/g lebih efektif dalam mempercepat waktu penyembuhan luka bakar dibandingkan dengan kontrol positif atau pemberian obat sintetik (bioplacenton 10%).

Efek Antifertilitas Ekstrak Mangrove Bruguiera gymnorrhiza Lamk. Terhadap Kualitas Spermatozoa Mencit (Mus musculus L.) Jamili Jamili; Wa Ode Harlis; Suriana Suriana; Siska Pratiwi
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 2 (2021): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Tujuan penelitian ini untuk mengetahui efek antifertilitas ekstrak mangrove Bruguiera gymnorrhiza terhadap kualitas spermatozoa mencit (Mus musculus L.). 24 ekor mencit dibagi dalam 4 kelompok perlakuan yaitu : Kontrol, DN (ekstrak daun), KB (ekstrak kulit batang) dan BH (ekstrak buah) mangrove 20 mg/bb. Ekstrak diberikan secara oral setiap hari selama 34 hari. Pada hari ke-35 mencit dibius dengan klorofom selanjutnya dikorbankan dan dilakukan pengambilan cauda epididymis untuk pengamatan kualitas spermatozoa mencit. Parameter kualitas spermatozoa yang diamati yaitu persentase morfologi spermatozoa abnormal, persentase viabilitas spermatozoa dan persentase motipitas spermatozoa. Data dianalisis dengan ANOVA dan uji BNT (α=0,05%). Hasil penelitian menunjukkan bahwa pemberian ekstrak mangrove Bruguiera gymnorrhiza secara signifikan menurunkan kualitas spermatozoa mencit. Penurunan motilitas terbanyak terjadi pada DN (20,6%) dibandingkan dengan KB (83,1%) dan BH (80,3%). Persentase rata-rata morfologi spermatozoa normal pada semua perlakuan adalah kontrol (85,1%), DN (15,1%), KB (58,8%) dan BH (62,5%). Persentase rata-rata viabilitas spermatozoa mencit hidup adalah kontrol (81,5%), DN (24,6%), KB (17,1%) dan BH (24,1%). Dari hasil penelitian dapat disimpulkan bahwa ekstrak mangrove Bruguiera gymnorrhiza memiliki efek antifertilitas karena menyebabkan penurunan kualitas spermatozoa dengan meningkatkan persentase morfologi spermatozoa abnormal, persentase viabilitas dan menurunkan persentase motilitas spermatozoa.Kata kunci : Ekstrak, Bruguiera gymnorrhiza, Motilitas Spermatozoa, Morfologi Spermatozoa, Viabilitas Spermatozoa Antifertility Effects Mangrove Extract Bruguiera gymnorrhiza Lamk. Against Spermatozoa Quality of Mice (Mus musculus L.) ABSTRACTThe purpose of this study was to determine the antifertility effect of Bruguiera gymnorrhiza mangrove extract on the sperm quality of mice (Mus musculus L.). 24 mice were divided into 4 treatment groups, namely: control, DN (leaf extract), KB (bark extract) and mangrove fruit extract (BH) 20 mg/ww. The extract was administered orally every day for 34 days. On the 35th day, the mice were anesthetized with chloroform, then sacrificed and the cauda epididymis was taken to observe the sperm quality of the mice. The parameters of spermatozoa quality observed were the percentage of abnormal spermatozoa morphology, the percentage of spermatozoa viability and the percentage of spermatozoa motility. Data were analyzed by ANOVA and BNT test (α=0,05%). The results showed that the administration of Bruguiera gymnorrhiza mangrove extract significantly reduced the sperm quality of mice. The greatest decrease in motility occurred in DN (20.6%) compared to KB (83.1%) and BH (80.3%). The average percentage of normal spermatozoa morphology in all treatments was control (85.1%), DN (15.1%), family planning (58.8%) and BH (62.5%). The average percentage of spermatozoa viability of live mice was control (81.5%), DN (24.6%), KB (17.1%) and BH (24.1%). From the results of the study, it can be concluded that the mangrove extract of Bruguiera gymnorrhiza has an antifertility effect because it causes a decrease in the quality of spermatozoa by increasing the percentage of abnormal spermatozoa morphology, the percentage of viability and decreasing the percentage of spermatozoa motilityKeyword : Extracts, Bruguiera gymnorrhiza, Motility Spermatozoa, Morphology Spermatozoa, Viability Spermatozoa

EFEKTIVITAS EKSTRAK RIMPANG JAHE PUTIH (Zingiber officinale var. Amarum) SEBAGAI ANTIFERTILITAS TERHADAP KEBUNTINGAN MENCIT (Mus musculus L.) TAHAP PRAIMPLANTASI Wa Ode Harlis; Nurhayu Malik; Nufrianti Nufrianti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 5, No 1 (2018): Sains & Biodiversitas Wallacea
Publisher : University of Halu Oleo

ABSTRAKTujuan penelitian ini untuk melihat efektivitas ekstrak rimpang jahe putih (Zingiber officinale var. Amarum) sebagai antifertilitas terhadap kebuntingan mencit (Mus musculus L.) tahap praimplantasi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan. 16 ekor mencit betina berat 20-30 gr berumur 2-3 bulan dibagi kedalam 4 kelompok yaitu; K1 (Aquades), K2 (0,7 mg/g bb), K3 (1,4 mg/g bb), dan K4 (2,8 mg/g bb) ekstrak. Pemberian Ekstrak diberikan secara oral sebanyak 0,5 ml/ekor/hari pada kebuntingan hari ke-0 sampai ke-3. Mencit dipelihara sampai hari kebuntingan ke-18. Mencit dikorbankan dan dibedah, selanjutnya diamati parameter kebuntingan tahap praimplantasi. Hasil penelitian menunjukkan bahwa ekstrak rimpang jahe putih menurunkan persentase implantasi yaitu K1 (0,90%), K2 (0,39%), K3. Menurunkan persentase embrio resorbsi yaitu K1 (0%), K2 (0%), K3. Menurunkan persentase fetus hidup yaitu K1 (0,90%), K2 (0,35%), K3. Serta meningkatkan persentase fetus mati K1 (0%), K2 (0,04%) K3 dan pada keempat parameter untuk K4 tidak ditemukan fetus, Ekstrak juga meningkatkan persentase kehilangan gestasi yaitu K1 (0,12%), K2 (0,60%), K3 (1%)dan K4 (1%). Kata kunci : Ekstrak rimpang jahe putih, Kebuntingan tahap praimplantasi ABSTRACT The purpose of this study was to see the effectiveness of white ginger rhizome extract (Zingiber officinale var. Amarum) as an antifertility on pregnancy in the preimplantation stage of mice (Mus musculus L.). This study uses a Completely Randomized Design (CRD) consisting of 4 treatments. 16 female mice weighing 20-30 grams aged 2-3 months were divided into 4 groups, namely; K1 (Aquades), K2 (0.7 mg / g bb), K3 (1.4 mg / g bb), and K4 (2.8 mg / g bb) extract. The administration of the extract was administered orally as much as 0.5 ml / head / day in the 0 to 3 day pregnancy. Mice are maintained until the 18th pregnancy day. Mice were sacrificed and dissected, then the preimplantation stage pregnancy parameters were observed. The results showed that the white ginger rhizome extract reduced the percentage of implantation, namely K1 (0.90%), K2 (0.39%), K3. Reducing the percentage of resorbsi embryos, namely K1 (0%), K2 (0%), K3. Reducing the percentage of live fetuses, namely K1 (0.90%), K2 (0.35%), K3. As well as increasing the percentage of dead fetuses K1 (0%), K2 (0.04%) K3 and in the fourth parameter for K4 no fetus was found, the extract also increased the percentage of gestational loss, namely K1 (0.12%), K2 (0.60% ), K3 (1%) and K4 (1%). Keywords: White ginger rhizome extract, preimplantation stage pregnancy

Spermatogenesis Mencit (Mus musculus, L.) Pasca Pemberian Ekstrak Brotowali (Tinospora crispa, L.) Wa Ode Harlis; Andi Septiana; Arjuni Arjuni
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 1 (2019): Biology and Wallacea
Publisher : University of Halu Oleo

ABSTRAKBrotowali (Tinospora crispa, L.) merupakan tumbuhan yang mengandung senyawa yang tergolong dalam kelompok antifertilitas diantaranya golongan glikosida, alkaloid, flavonoid, saponin dan tanin. Kelompok senyawa tersebut dapat memberikan efek sitotoksik pada reproduksi jantan dengan mengganggu metabolisme sel germinal dan sel spermatogenik. Tujuan penelitian ini untuk mengetahui spermatogenesis mencit pasca diberikan ekstrak brotowali. Sebanyak 20 ekor mencit jantan berat 30-40 gr, berumur 2-3 bulan dibagi 5 kelompok perlakuan yaitu; K1 kontrol positif (aquades), K2 kontrol negatif (Na CMC 0,5% ), K3 (0,05 g/g bb), K4 (0,06 g/g bb), dan K5 (0,07 g/g bb) ekstrak brotowali. Ekstrak diberikan secara oral setiap hari selama 34 hari. Pada hari ke-35 mencit dikorbankan dengan kloroform selanjutnya pengambilan organ testis untuk dibuat preparat mikroanatomi. Data dianalisis dengan ANOVA dan uji BNT (α=0,05%). Hasil penelitian menunjukkan bahwa pemberian ekstrak tumbuhan brotowali secara signifikan mengganggu proses spermatogenesis mencit dengan menurunkan jumlah sel spermatogonium, spermatosit primer, sel spermatid dan kepadatan spermatozoa. Penurunan jumlah sel-sel spermatogenik dalam penelitian ini terbanyak pada perlakuan K5 untuk semua kelompok, dengan jumlah spermatogonium (28,5), dengan jumlah spermatosit primer (33,3), dengan jumlah spermatid (69,4) dan kepadatan spermatozoa (1,19). Kata kunci : Ekstrak, Tinospora crispa, L., Spermatogenesis, Mencit ABSTRACTBrotowali (Tinospora crispa, L.) is a plant that contains compounds belonging to the antifertility group including glycosides, alkaloids, flavonoids, saponins and tannins. This group of compounds can provide cytotoxic effects on male reproduction by disrupting germinal cell metabolism and spermatogenic cellsThe aim of this study was to know the effect of extracts Tinospora crispa, L. on spermatogenesis of mice (Mus musculus, L.). The male mice were used 20 with weight 30-40 g, aged 2-3 months, and were treated in 5 groups i.e. K1 : positive control (aquadest), K2 : negative control (Na CMC 0.5%), K3 (0.05 g/g bb), K4 : (0.06 g/g bb), and K5 (0.07 g/g bb) extracts of brotowali. The extracts were given orally during 34day. At the 35th the weight of mice werw counted and then sacrificed with chloroform, and the testis were collected to make microanatomy. The data were analyzed with ANOVA (Analysis of Varience) and t-Test (α = 0,05%). The results showed that the treatments with brotowali extracts obstructed spermatogenesis process and reduced the amount of spermatogonium cells, primary spermatocyt, spermatide cells and density of spermatozoa with increasing dosage of brotowali extracts. The decreasing of spermatogenic cells were observed in dosage of K5 with spermatogonium cells (28,5), the amount primary spermatocyte (33,3), the amount of spermatide (69,4) and the density of spermatozoa (1,19).Key word : Extracts, Tinospora crispa, L., Spermatogenesis, Mice

Kadar Asam Urat Mencit Hiperurisemia Setelah Pemberian Perasan Daun Kersen (Muntingia calabura L.) dan Daun Kelor (Moringa oleifera L.) Wa Ode Harlis; Sitti Wirdhana Ahmad; Jusrianti Jusrianti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 7, No 1 (2020): BioWallacea and Sains
Publisher : University of Halu Oleo

The quercetin flavonoid content in cherry leaves and Moringa serves as a competitive inhibitor of hypoxanthine and xanthine which causes uric acid not to form. The purpose of this study was to determine the effect of grains of cherry leaves and Moringa leaves on the uric acid levels of mice. 25 male mice weighing 20-30 grams 2-3 months old were divided into 5 treatment groups, namely: K1 as a negative control (without treatment), K2 as the positive control (chicken liver juice 0.5 mL), K3 (cherry leaf juice and liver juice chicken), K4 (Moringa leaf juice and chicken liver juice) and K5 (a mixture of cherry leaf juice, Moringa, and chicken liver juice). Mice are first measured initial uric acid levels. The administration of chicken liver juice at a dose of 0.5 mL is done three times a day for 7 days so as to achieve a hyperuricemia condition. Furthermore, the juice was given according to the treatment group at a dose of 0.2 mL for 14 days and finally, uric acid levels were calculated. Data were analyzed by ANOVA and LSD test (α = 0.05). The results showed that the administration of cherry leaves and moringa juice significantly reduced blood uric acid levels in mice in all treatment groups. The highest mean decrease in uric acid levels was in the K5 (0.91 mg / dL) group, compared with K1 (0.93 mg / dL), K2 (2.03 mg / dL), K3 (0.994 mg / dL) and K4 (0.98 mg / dL) .Keywords: Uric Acid Levels, Hyperuricemia, Munitingia calabura L, Moringa oleifera L. AbstrakKandungan flavonoid golongan kuercetin pada daun kersen dan kelor berfungsi sebagai inhibitor kompetitif hipoxantin dan xantin yang menyebabkan asam urat tidak terbentuk. Tujuan penelitian ini untuk mengetahui pengaruh pemberian perasan daun kersen dan daun kelor terhadap kadar asam urat mencit. 25 ekor mencit jantan berat 20-30 gr berumur 2-3 bulan dibagi kedalam 5 kelompok perlakuan yaitu: K1 sebagai kontrol negatif (tanpa perlakuan), K2 sebagai kontrol positif (jus hati ayam 0.5 mL), K3 (perasan daun kersen dan jus hati ayam), K4 (perasan daun kelor dan jus hati ayam) dan K5 (campuran perasan daun kersen, kelor dan jus hati ayam). Mencit terlebih dahulu diukur kadar asam urat awal. Pemberian jus hati ayam dengan dosis 0.5 mL dilakukan tiga kali sehari selama 7 hari sehingga mencapai kondisi hiperurisemia. Selanjutnya diberi perasan sesuai kelompok perlakuan dengan dosis 0.2 mL selama 14 hari dan dihitung kadar asam urat akhir. Data dianalisis dengan ANOVA dan uji BNT (α=0,05). Hasil penelitian menunjukkan bahwa pemberian perasan daun kersen dan kelor secara signifikan menurunkan kadar asam urat darah mencit pada semua kelompok perlakuan. Rerata penurunan kadar asam urat tertinggi terdapat pada kelompok K5 (0.91 mg/dL), dibandingkan dengan K1 (0.93 mg/dL), K2 (2.03 mg/dL), K3 (0.994 mg/dL) dan K4 (0.98 mg/dL).Kata kunci : Kadar Asam Urat, Hiperurisemia.,Munitingia calabura L, Moringa oleifera L.

GAMBARAN HISTOLOGI TESTIS MENCIT (Mus musculus, L.) SETELAH PEMBERIAN EKSTRAK TUMBUHAN BROTOWALI (Tinospora crispa, L.) Wa Ode Harlis; Andi Septiana
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 4, No 1 (2017): Biosains & Technology in Wallacea
Publisher : University of Halu Oleo

ABSTRAK Tumbuhan Brotowali (Tinospora crispa, L.) diketahui mengandung senyawa yang tergolong dalam kelompok antifertilitas diantaranya golongan glikosida, alkaloid, flavonoid, saponin dan tanin. Kelompok senyawa-senyawa tersebut dapat memberikan efek sitotoksik pada reproduksi jantan dengan mengganggu metabolisme sel germinal dan sel spermatogenik. Tujuan penelitian ini untuk mengetahui pengaruh ekstrak brotowali terhadap spermatogenesis mencit. 20 ekor mencit jantan berat 20-30 gr, berumur 2-3 bulan dibagi 5 kelompok yaitu; K1 kontrol negatif (aquades), K2 kontrol positif (Na CMC 0,5% ), K3 (0,05 g/g bb), K4 (0,06 g/g bb), dan K5 (0,07 g/g bb) ekstrak brotowali. Ekstrak diberikan secara oral selama 34 hari. Pada hari ke-35 dikorbankan dan dilakukan pengambilan organ testis untuk preparat mikroanatomi. Penelitian bersifat deskriptif, data disajikan dalam bentuk gambar. Berdasarkan hasil penelitian menunjukkan bahwa pada penampang melintang testis nampak jelas terjadi penurunan jumlah sel sel spermatogenik dalam tiap penampang tubulus seminiferous mencit, pada semua perlakuan yang diberikan ekstrak tumbuhan brotowali seiring dengan meningkatnya dosis yang diberikan Kata Kunci : Tinospora crispa, L., histologi testis, Mencit ABSTRACTThe Brotowali plant (Tinospora crispa, L.) is known to contain compounds belonging to the antifertility group such as glycosides, alkaloids, flavonoids, saponins and tannins.These groups of compounds may have a cytotoxic effect on male reproduction by disrupting the metabolism of germ cells and spermatogenic cellsThe aim of this study was to knowthe effect of extracts Tinospora crispa, L. on spermatogenesis of mice. The male mice were used 20 weight 30-40 g, aged 2-3 months, and were treated in 5 groups i.e. K1 : negative control (aquadest),K2 : positive control (Na CMC 0.5%), K3 (0.05 g/g bb), K4 : (0.06 g/g bb), and K5 (0.07 g/g bb) extracts of brotowali. The extracts were given orally during 34 day.At the 35 th the testis were collected to make microanatomy. Research is descriptive, data is presented in the form of picture. Based on the results showed that crosssectional testis clearly visible decrease in the number of spermatogenic cell cells in each section of tubular seminiferous mice, in all treatments given extracts of brotowali plants along with increased doses given.

Aktivitas Antibakteri Gel Daun Sembung Legi (Blumea balsamifera L.) Sebagai Sediaan Penyembuh Luka Pada Mencit (Mus musculus L.) : Antibacterial Activity of Sembung Legi Leaf Gel (Blumea balsamifera L.) As a Wound Healing Preparation in Mice (Mus musculus L.) Wa Ode Harlis; Muh. Hajrul Malaka; Alfiawin Alfiawin
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 12 No. 2 (2022): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v12i2.206

Abstract The purpose of this study was to determine the antibacterial activity of sembung legi leaf gel (Blumea balsamifera L.) and its effect on wound healing in mice (Mus musculus L.). The leaves of sembung legi are first extracted by maceration and then made gel preparations with a formula of 3%, 4%, and 5%. Parameters in this research is antibacterial test against Staphylococcus aureus bacteria and the length of time for wound healing. The activity of antibacterial is tested by measuring the diameter of the zone of inhibition against the growth of S.aureus bacteria. Gel test as a wound healing preparation was carried out on mice.A total of 15 mice aged 2 - 3 months were divided into 5 treatment groups, namely negative control (without treatment), positive control (Kalmicetine 10%), K1 (3% gel), K2 (4% gel), and K3 (5% gel). The back of the mice was injured with a 1 cm incision, then treated by sembung legi leaf gel 3 times a day for 14 days. Wound healing time data were analyzed by ANOVA and LSD test. The results showed that sembung legi leaf gel could inhibit the growth of S.aureus bacteria with an average diameter of the clear zone for each treatment, namely KN (0.0 mm), KP (30.15 mm), K1 (4.77 mm), K2 ( 4.81 mm), and K3 (6.31 mm). The parameters of the mean length of time for wound healing with healing parameters were the absence of erythema, swelling, wound closure, and healing for all treatments, namely KN (12.3 days), KP (8.3 days), K1 (11.3 days), K2 (10.6 days), and K3 (9.6 days). It is concluded that sembung legi leaf gel can inhibit the growth of S. aureus bacteria and speed up wound healing time, so that it can be used as an alternative gel preparation in wound healing. Keywords: Antibacterial activity; Leaf Blumea balsamifera L.; Mice; Staphylococcus aureus; Wound healing. Abstrak Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri gel daun sambung legi (Blumea balsamifera L.) dan pengaruhnya terhadap penyembuhan luka pada mencit (Mus musculus L.). Daun sembung legi dimaserasi dan dibuat sediaan gel dengan formula 3%, 4%, dan 5%. Penelitian ini menguji antibakteri S. aureus dengan mengukur diameter zona hambat terhadap pertumbuhan bakteri dan lama waktu penyembuhan luka. Uji gel sebagai sediaan penyembuh luka dilakukan terhadap hewan uji mencit. Sebanyak 15 ekor mencit berumur 2 – 3 bulan dibagi ke dalam 5 kelompok perlakuan yaitu kontrol negatif (tanpa perlakuan), kontrol positif (Kalmicetine 10%), K1 (gel 3%), K2 (gel 4%), dan K3 (gel 5%). Punggung mencit dilukai dengan sayatan sepanjang 1 cm, kemudian dioleskan gel daun sembung legi sebanyak 3 kali sehari selama 14 hari. Data lama waktu penyembuhan luka dianalisis dengan ANOVA dan uji LSD. Hasil penelitian menunjukkan bahwa gel daun sembung legi dapat menghambat pertumbuhan bakteri S. aureus dengan rata-rata diameter zona bening setiap perlakuan yaitu KN (0,0 mm), KP (30,15 mm), K1 (4,77 mm), K2 (4,81 mm), dan K3 (6.31 mm). Rerata lama penyembuhan luka dengan parameter penyembuhan berupa tidak adanya eritema, pembengkakan, luka menutup, dan sembuh untuk semua perlakuan yaitu KN (12.3 hari), KP (8,3 hari), K1 (11,3 hari), K2 (10,6 hari), dan K3 (9,6 hari). Kesimpulan: Gel daun sembung legi dapat menghambat pertumbuhan bakteri S. aureus dan mempercepat waktu penyembuhan luka, sehingga dapat dijadikan sebagai sediaan gel alternatif dalam penyembuhan luka. Kata Kunci : Aktivitas antibakteri; Daun Blumea balsamifera L.; Mencit; Penyembuhan luka; Staphylococcus aureus.

Efektivitas Fotodegradasi Lignin dari Limbah Ampas Sagu (Metroxylon sagu Rottb.) Menggunakan Katalis TiO₂: The Effectiveness of the Photodegradation of Lignin from Sago (Metroxylon sagu Rottb.) Waste Using TiO₂ Catalyst Muh. Natsir; Aden Mula Pratiwi; Thamrin Azis; Nohong; Imran; Wa Ode Harlis; Alimin; La Ode Kadidae; Ruslan; C Bijang; La Ode Abd Kadir; Laily Nurliana
KOVALEN: Jurnal Riset Kimia Vol. 8 No. 3 (2022): December Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2022.v8.i3.16149

Lignin degradation from sago (Metroxylon sagu Rottb.) waste has been carried out using a TiO2 catalyst. This research aims to determine the effectiveness of lignin degradation from sago waste using a TiO2 catalyst. Lignin from sago pulp was isolated using 10% NaOH and characterized using Fourier Transform Infra Red (FTIR) spectroscopy. The results of the characterization using FTIR show that the absorption at wave number 2937.59 cm-1 is the -C-H stretching vibration of the alkane functional groups, the absorption at wave number 2360.87 cm-1 is the vibration of the C≡C triple bond, the wave numbers 1795.73 cm-1 and 1637.56 cm-1 are associated with the stretching of the carbonyl group. The absorption at wave number 1427.32 cm-1 is a C-H vibration connected to an unsaturated bond in an aromatic ring. Absorptions at wave number 1105.21 cm-1, 1128.36 cm-1, 1153.43 cm-1 were the stretching vibration of -C-H on the guaiasil ring, and the absorption at wave number 1022.27 cm-1 was the stretching vibration of C-O-C ether. From this spectrum, it can be seen that lignin is not completely pure because it is probably still mixed with cellulose. The results of the effectiveness test of lignin degradation using a TiO2 catalyst with the help of UV light were able to degrade the lignin isolated by 31.43%, for 3 hours at a lignin concentration of 40 ppm.

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