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All Journal bionature AL KAUNIYAH BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati SEMIRATA 2015 BioWallacea Journal of Biological Research agriTECH Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL BIOLOGI INDONESIA BIOMA : Jurnal Biologi Makassar ALCHEMY Jurnal Penelitian Kimia Jurnal Pengabdian Pada Masyarakat SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Biosaintifika: Journal of Biology & Biology Education Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Berkala Penelitian Agronomi Jurnal ABDINUS : Jurnal Pengabdian Nusantara IJOEP : International Journal of Ecophysiology Jurnal Pengabdian UNDIKMA Jurnal Biofiskim: Pendidikan dan Pembelajaran IPA Molekul: Jurnal Ilmiah Kimia Majalah Pengabdian Indonesia Jurnal Pusat Kolaborasi Pengabdian Kepada Masyarakat Bionature

Nur Arfa Yanti

Jurusan Biologi FMIPA Universitas Halu Oleo, Kendari

Author-ID : 333721

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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SCREENING BAKTERI AMILOLITIK DAN SELULOLITIK DARI LIMBAH SAGU (Screening of Amylolytic and Cellulolytic Bacteria From Sago waste) Yanti, Nur Arfa; Munir, Asmawati
Jurnal BioWallacea Vol 1, No 1 (2014)
Publisher : Jurnal BioWallacea

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Screening of indigenous bacteria from sago waste based on amylolytic and cellulolytic activity was done to obtain bacterial isolate having double activity, i.e. could to hydrolize of starch (amylolytic) and cellulose (cellulolytic). Screening amylolytic and cellulolytic bacteria was done based on amylolytic and cellulolytic activity on agar media. Determination of amylolytic activity on starch agar media was based on the presence of clear zone around the bacterial colony upon flooding with lugolâs iodine solution. Cellulolytic activity was determine based on the presence of clear zone around the bacterial colony on Carboxy methyl cellulose (CMC) agar upon flooding with congo red solution. Presence of a clear zone around the colony indicated starch and cellulose hydrolysis. The diameters of clear zone produced on CMC and starch agar were measured and used as an indication of the amylolytic and cellulolytic activities of the bacteria. The results of the screening based on amylolytic and cellulolytic activity showed that a number of 21 bacterial isolates that having both activities. LCA2 was the bacterial isolate with the highest amylolytic and cellulolytic activity as revealed by the size of clearing zone on both types of agar plates. The diameters of clear zone on starch and CMC agar were 4,98Â Â and 3,65 cm2, respectively. Therefore, LCA2 isolate was bacterial isolate that potent for biconvertion sago hampas into value-added products. Keywords : Bacteria, Amylolytic, Cellulolytic, Sago waste.

PRODUKSI BIOPLASTIK DARI PATI SAGU OLEH BAKTERI AMILOLITIK LOKAL MENGGUNAKAN SUMBER NITROGEN BERBEDA H. Muhiddin, Nur Arfa Yanti, Nurhayani
SEMIRATA 2015 Prosiding Bidang Biologi
Publisher : SEMIRATA 2015

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Poli-Î²-hidroksibutirat (PHB) merupakan bioplastik yang disintesis oleh bakteri dan berpotensi digunakan sebagai pengganti plastik sintetik. Penggunaan pati sagu sebagai sumber karbon murah dapat membantu menurunkan biaya produksi bioplastik PHB. Pengaruh sumber nitrogen yang berbeda pada produksi bioplastik PHB dari pati sagu oleh bakteri amilolitik lokal, Bacillus megaterium PSA10 dikaji dalam penelitian ini. Sumber Nitrogen yang digunakan dalam penelitian ini adalah pepton, yeast ekstrak, protease pepton, (NH4)2SO4 dan NH4NO3. Kadar PHB dideterminasi menggunakan metode spektrofotometri. Produksi PHB dari pati sagu dan pertumbuhan B. megaterium PSA10 tertinggi diperoleh dengan menggunakan (NH4)2SO4 sebagai sumber N yaitu berturut-turut 50,55 % (g PHB/g berat kering sel) dan 1,82 g berat kering sel/L Konsentrasi (NH4)2SO4 sebanyak 1 g/L (0,1%) mampu menghasilkan bioplastik PHB yang tertinggi dengan kadar PHB 51,32 %. Dengan demikian, penggunaan (NH4)2SO4 sebagai sumber nitrogen sebanyak 0,1 % sangat baik untuk memproduksi PHB dari pati sagu oleh bakteri amilolitik lokal. Kata kunci: Bioplastik, PHB, pati sagu, sumber Nitrogen, bakteri amilolitik Â

ANALISIS BAKTERI KOLIFORM DAN PATOGEN DEPOT AIR MINUM KECAMATAN MANDONGA KOTA KENDARI Aswan, Muhammad Aswan; Darlian, Lili; Yanti, Nur Arfa
Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Vol 1, No 1 (2017): Seminar Nasional Riset Kuantitatif Terapan 2017
Publisher : Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017

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Refill drinking water depot used primarily in sub-district community Mandonga. Which is indicative of contamination of drinking water refill that contamination of coliform bacterial and pathogenic bacteial. The aim ofÂ the study was to determine the microbiological quality of drinking water refill in District Mandonga. In this work, carry out were analyzed technique quantitatively and qualitatively by testing the Presumtive test, Confirmed test and Complied test as well as using specific media. The result suggest that, there is a negative one, namely coliform bacterial from the village and there are four depots Anggilowu positive coliform bacterial that is depot in the village of Alolama, Wawombalata, Labibia and Korumba, with the highest MPN value found in the depot Korumba and Wawombalata is > 1100 MPN/100 mL, whereas in the Alolama village is 240 MPN / 100 mL and in the Wawombalata villageÂ is 460 MPN/ 100 mL to MPN a value. The result of indicate that bacterial amplifier contamintaion of the depot is a non-faecal colifom bacteria (Enterobacter). Based on the test result found pathogenic bacteria Vibrio sp. at the depot in the village Alolama, Wawombalata and Labibia.KeywordsÂâ€” Coliform Bacterial, Pathogen, The Quality of Drinking Water Depots

Screening of Acetic Acid Bacteria from Pineapple Waste for Bacterial Cellulose Production using Sago Liquid Waste Yanti, Nur Arfa; Ahmad, Sitti Wirdhana; Ambardini, Sri; Muhiddin, Nurhayani Haji; Sulaiman, La Ode Iman
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10241

Bacterial cellulose is a biopolymer produced by fermentation process with the help of bacteria. It has numerous applications in industrial sector with its characteristic as a biodegradable and nontoxic compound in nature. The potential application of BC is limited by its production costs, because BC is produced from expensive culture media. The use of cheap carbon and nutrient sources such as sago liquid waste is an interesting strategy to overcome this limitation. The objective of this study was to obtain the AAB strain that capable to produce bacterial cellulose from sago liquid waste. Isolation of AAB strains was conducted using CARR media and the screening of BC production was performed on Hestrin-Schramm (HS) media with glucose as a carbon source. The strains of AAB then were evaluated for their cellulose-producing capability using sago liquid waste as a substrate. Thirteen strains of AAB producing BC were isolated from pineapple waste (pineapple core and peel) and seven of them were capable to produce BC using sago liquid waste substrate. One of the AAB strains produced a relatively high BC, i.e. isolate LKN6. The result of morphological and biochemical test was proven that the bacteria was Acetobacter xylinum. The result of this study showed that A. xylinum LKN6 can produce a high yield of BC, therefore this strain is potentially useful for its utilization as a starter in bacterial cellulose production.

PEMANFAATAN LIMBAH CAIR SAGU UNTUK MEMPRODUKSI SELULOSA BAKTERI Ahmad, Sitti Wirdhana; Yanti, Nur Arfa; Muhiddin, Nurhayani H.
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3763

ABSTRACTBacterial cellulose is an exopolysaccharide produced by bacteria and has a high purity level compared to plant cellulose and has unique structural and mechanical characteristics that can be utilized for various industrial purposes such as food, medical, plastic and paper. This study aims to determine the potential of sago liquid waste as a substrate for producing biocellulose and sugar concentration is required in producing bacterial cellulose from sago liquid waste. Production of bacterial cellulose from sago liquid waste was done with static condition for 14 days with treatment of sugar concentration 5,10,15 and 20% (w/v) using Acetobacter xylinum. Parameters were measured include of thickness, yield, crude fiber content and moisture content. Production of bacterial cellulose using sago liquid waste requires the addition of sugar as much as 10% with a thickness of 21.73 mm, yield of 34.97%, crude fiber of 4.5% and moisture content of 91.35%. Therefore, sago liquid waste is potentially used as a substrate for producing bacterial cellulose. Keywords :Biocellulose, nata, Acetobacter xylinum, Production substrate

ISOLASI DAN IDENTIFIKASI KAPANG PELARUT PHOSPHATE DARI RIZOSFER GADUNG (DIOSCOREA HISPIDA DENNST) DAN BAMBU (DENDROCALAMUS ASPER) Suleman, Darwis; Sani, Asrul; Ambardini, Sri; Yanti, Nur Arfa; Boer, Dirvamena
Berkala Penelitian Agronomi Vol 7, No 2 (2019)
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/bpa.v7i2.10564

Fosfor (P) merupakan hara utama kedua yang membatasi produksi tanaman, namun ketersediaannya di dalam tanah sangat masalah karena reaksi presipitasi dengan Al+3 dan Fe+3 pada tanah masam atau Ca+2 pada tanah alkalin. Selama beberapa tahun terakhir, pemanfaatan mikroba tanah di anggap sebagai salah satu alternatif untuk meningkatkan ketersediaan P untuk tanaman. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi kapang pelarut fosfat dari rizosfer bambu dan gadung. Isolasi kapang pelarut fosfat dilakukan pada kondisi in vitro, melalui serangkaian pengenceran dengan menggunakan media Pikovskaya padat. Hasil penelitian diperoleh dua isolat dari rizosfer gadung dan satu isolat dari rizosfer bambu. Namun, hasil uji kelarutan P hanya satu isolat dari gadung yang menunjukkan zona bening di sekitar koloni. Secara kualitatif, kelarutan P bervariasi antara 2,405 dan 2,052 untuk gadung dan bamboo. Analisis numerik-fenetik mengungkapkan bahwa isolat kapang dari gadung terdeteksi sebagai Talaromyces aculeatus dengan indeks kesamaan 100% dan isolat kapang dari bambu teridentifikasi sebagai Fuzarium proliferatum dengan indeks kesamaan 89,7% dibandingkan dengan kapang acuan.Kata kunci : Asam organik, kapang, phosphate, pelarut, rizosfer

ANALISI FAKTOR â€“ FAKTOR YANG MEMPENGARUHI PRESTASI BELAJAR BIOLOGI : STUDI KASUS PADA SISWA KELAS XI SMA DI KABUPATEN MOROWALI Septiana, Santi; Fahyuddin, Fahyuddin; Yanti, Nur Arfa
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Publisher : Halu Oleo University

Abstrak. Tujuan penelitian ini adalahÂ untuk mempelajari Pengaruh Lingkungan Keluarga, Lingkungan Sekolah, Minat Dan Motivasi Belajar Terhadap Prestasi Belajar Biologi Pada Siswa SMA Se-Kabupaten Morowali. Jenis penelitian ini adalah korelasi sebab akibat. Teknik pengambilan data menggunakan kuesioner dan dokumentasi. Populasi dalam penelitian ini berjumlah 2.630 siswa dan sampel berjumlah 169 siswa dengan teknik cluster stratified random sampling yang berasal dari siswa kelas XI IPA dari SMAN 1 Witaponda, SMAN 2 Bungku, SMAN 1 Menui Kepulauan, SMAS Al-Khairaat Kolono dan SMAN 1 Bungku Pesisir. Teknik analisis menggunakan teknik analisis jalur. Hasil penelitian menunjukkan bahwa variabel eksternal lingkunganÂ keluargaÂ danÂ lingkunganÂ sekolahÂ siswaÂ SMAÂ sekabupatenÂ MorowaliÂ masih tergolong sedang secara rata. Lingkungan keluarga, lingkungan sekolah, minat, dan motivasi secara bersama sama memberikan pengaruh yang signifikan pada prestasi belajar biologi sebesar 24% dan sisanya 76% di pengaruhi oleh faktor lain.Â Kata Kunci: lingkungan keluarga, lingkungan sekolah, minat, motivasi dan prestasi belajar

Analisis Kuantitatif Mikroorganisme pada Ragi Tape Lokal dan Daya Terima Tape Jusinta yang dihasilkan Nurhayani H. Muhiddin; Ramlawati Ramlawati; Nur Arfa Yanti; Abdul Mun'im
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

The purpose of this study were to analyze the quantity of microorganisms in some local ragi tape and determine the panelists' acceptance of the "tape jusinta" was produced. This type of research is an experiment with a solid substrate fermentation method. The used ragi tape is 4 kinds of local ragi tape from South Sulawesi Province (ragi tape A, B, C, and D) and 1 type of local ragi tape from Southeast Sulawesi Province (ragi tape E). The Tape Jusinta was produced by fermentation of a solid mixture of purple sweet potato and cassava tubers in a ratio of 1: 1 using 5 kinds of local ragi tape. Analysis of microbial quantity in ragi tape includes the number of molds, yeasts and bacteria in viable count using the Standard Plate Count (SPC) method. Panelist acceptance of the Tape Jusinta product was analyzed using the Hedonic test with a numerical scale range of "6". Data were analyzed descriptively and inferentially using Anova and Duncan's Multiple Test. The results of the data analysis showed that the level of palatability of the panelists to the tapejusinta fermentedragi tape B was not different from the tape jusinta fermented ragi tape A and C, but the tape jusinta fermented by Cragi tape was different from the results of fermentation ragi tape D and E. The “tape jusinta” fermented ragi tape B is preferred over the tape jusinta fermented ragi tape A, C, D and E with the highest average panelist rating of 4.15. The quantity of microorganisms on ragi tape B are mold types as much as 1.0 x 104 cfu / g, yeast 8.0 x 103 cfu / g and bacteria 5.7 x 104 cfu/ g. Keywords: cassava roots, purple sweet potatoes, ragi tape, "tape jusinta", palatabilityAbstrakTujuan penelitian adalah menganalisis kuantitas mikroorganisme pada beberapa ragi tape lokal dan mengetahui daya terima panelis terhadap “tape jusinta” yang dihasilkan. Jenis penelitian adalah eksperimen dengan metode fermentasi substrat padat. Ragi tape yang digunakan adalah 4 macam ragi tape lokal yang berasal dari Provinsi Sulawesi Selatan (ragi tape A, B, C, dan D) dan 1 macam berasal dari Provinsi Sulawesi Tenggara (ragi tape E). Tape Jusinta dihasilkan melalui fermentasi substrat padat campuran ubi jalar ungu dan umbi ubi kayu dengan perbandingan 1 : 1 menggunakan 5 macam ragi tape lokal. Analisis kuantitas mikroba pada ragi tape meliputi jumlah kapang, khamir dan bakteri secara viable countmenggunakan metode Standard Plate Count (SPC). Daya terima panelis terhadap produk Tape Jusinta dianalisis menggunakan uji Hedonik dengan rentang skala numerik “6”.Data dianalisis secara deskriptif dan inferensial dengan Anava dan Duncan’s Multiple Test. Hasil analisis data menunjukkan bahwa tingkat kesukaan (palatability) panelis terhadap tape jusinta hasil fermentasi ragi tape B tidak berbeda dengan tape jusinta hasil fermentasi ragi tape A dan C, namun tape jusinta hasil fermentasi ragi tape C berbeda dengan hasil fermentasi ragi tape D dan E. Tape jusinta hasil fermentasi ragi tape B lebih disukai daripada tape jusinta hasil fermentasi ragi tape A, C, D dan E dengan rata-rata penilaian panelis tertinggi yaitu 4.15. Kuantitas mikroorganisme pada ragi tape B yaitu jenis kapang sebanyak 1.0 x 104 cfu/g, khamir 8.0 x 103 cfu/g dan bakteri 5.7 x 104 cfu/g .Kata kunci: Umbi ubi kayu, ubi jalar ungu, ragi tape, “tape jusinta”, daya terima

Karakterisasi Morfologi Phytophthora sp. Asal Buah Kakao Desa Olo-oloho, Kabupaten Konawe, Sulawesi Tenggara Muzuni Muzuni; Haidin Haidin; Nur Arfa Yanti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 7, No 1 (2020): BioWallacea and Sains
Publisher : University of Halu Oleo

This study aimed to determine the morphological characteristics of Phytophthora sp. isolated from cocoa fruits from Olo-oloho Village, Konawe Regency, Southeast Sulawesi. Isolation of Phytophthora sp. carried out by the point method using V4 (Vegetable Juice Agar) media incubated at 27ºC for 24 hours. Morphological characterization of Phytophthora sp. included characterization of colony morphology and cell morphology. The results showed that the colony morphological characteristics were white colonies, cotton-like textures, the uneven edge of the colony, zoning and radial lines. The morphological characteristics of the cell had asexual spores in the form of sporangium and chlamydospores, hyphae are not aseptic, greenish-black zoospores, zoospores are round and double-flagged, and have sporangiophores. Keywords: Phytophthora sp., colony morphology, cell morphology

PENGARUH PENAMBAHAN GULA DAN NITROGEN PADA PRODUKSI NATA DE COCO Nur Arfa Yanti; Sitti Wirdhana Ahmad; Desty Tryaswaty; A. Nurhana
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 4, No 1 (2017): Biosains & Technology in Wallacea
Publisher : University of Halu Oleo

ABSTRAK Produk nata de coco merupakan makanan fungsional kaya serat yang dihasilkan oleh bakteri Acetobacter xylinum secara fermentasi menggunakan media air kelapa. Biosintesis nata de coco membutuhkan sumber karbon dan nitrogen. Penelitian ini bertujuan untuk mengetahui konsentrasi gula dan sumber nitrogen, ZA yang terbaik untuk memproduksi nata de coco. Produksi nata de coco dilakukan dengan menambahkan gula dengan perlakuan konsentrasi 2; 3,5; 5 dan 7,5% (b/v), dan ZA dengan perlakuan konsentrasi 0,2; 0,35; 0,5 dan 0,75% (b/v). Parameter yang diamati adalah ketebalan nata de coco yang diukur menggunakan jangka sorong dan rendemen nata. Hasil penelitian menunjukkan bahwa penambahan gula dan ZA pada media fermentasi berpengaruh terhadap produksi nata de coco. Konsentrasi gula 5% dan ZA 0,5% yang terbaik menghasilkan nata de coco. Kata Kunci : Nata de coco, Gula, Nitrogen, ZA. ABSTRACT Nata de coco is a functional food rich of fiber produce by Acetobacter xylinum bacteria by fermented using coconut water media. The biosynthesis of nata de coco requires a source of carbon and nitrogen. This study aims to determine is the best concentration of sugar and nitrogen source, ZA for producing nata de coco. The production of nata de coco was done by adding sugar with the treatment of concentration 2; 3.5; 5 and 7.5% (w / v), and ZA with a treatment concentration of 0.2; 0.35; 0.5 and 0.75% (w/v). The observed parameters include the thickness of nata de coco which was measured using calipers and nata yield. The results showed that the addition of sugar and ZA in the fermentation media affect the production of nata de coco. The concentration of sugar 5% and ZA 0.5% is the best to produce nata de coco . Keywords : Nata de coco, sugar, Nitrogen, ZA

Co-Authors A. Nurhana Abdul Mun'im Adi Parman Rudia, La Ode Ahmad, Sitti Wirdhana Alfin Alfin Amirullah -, Amirullah Andi Septiana Ardiansyah ARDIANSYAH ARDIANSYAH Ardiansyah Ardiansyah Ardiansyah Ardiansyah Ardiansyah Ardiansyah Asmawati Munir, Asmawati Asrul Sani Aswan, Muhammad Aswan Budiman, Herdi Cahyanti, Kartika Dwi Dedy Oetama Desty Tryaswaty DIRVAMENA BOER, DIRVAMENA Erni Martani Fahyuddin Fahyuddin, Fahyuddin Fatimah Alwi Albakar Haidin Haidin Haji Muhiddin, Nurhayani Handayani Hamid, Fitri Ida Usman Ida Usman Indrawati Indrawati Irnawati Irnawati Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamili Jamili Karya, Adi Kurnia Yati La Ode Adi Parman Rudia La Ode Adi Parman Rudia La Ode Ahmad Nur Ramadhan La Ode Iman Sulaiman La Ode Kadidae, La Ode Langkah Sembiring Lili Darlian, Lili M. Jahiding M. Rajab Sutra Mijaya Marlina, Wa Ode Leni Marwati Arji Mashuni Mashuni Mashuni Pallawagau Muhammad Jahiding Muhiddin, Nurhayani Haji Muhsin Muhsin Muhsin Muhsin Muhsin Muhsin Muksar Muksar Muzuni, Muzuni Nurhayani H. Muhiddin Nurhayani H. Muhiddin, Nurhayani H. Nurhayani Haji Muhiddin Prasetya, Wandy Murti Ramlawati, Ramlawati Santi Septiana, Santi Sebastian Margino Sebastian Margino Sitti Wirdhana Ahmad Sitti Wirdhana Ahmad Sri Ambardini Sri Ambardini Sri Noegrohati Sulaiman, La Ode Iman Suleman, Darwis Surachma, Wahyuni Nurul Suriana Suriana Suriana Suriana Suriana Suriana Syah, Muhamad Azwar Taufik Walhidayah Vidya Nur Riska Parakkasi Wa Ode Isra Wa Ode Nanang Trisna Dewi Wa Ode Sitti Nurhaliza Wahyu Ahwarul Asis Wina Rezky

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