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Body Weight, Oocyte Elimination and Blood Profile of Rabbit After Challenge Test Using Eimeria stiedai Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Fitriyani, Nur; Munawaroh, Sitie; Hikmawati, Nita; Irawan, Desika Indra
ANIMAL PRODUCTION Vol 20, No 1 (2018)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.469 KB) | DOI: 10.20884/1.jap.2018.20.1.621

Abstract

The objective of the research was to investigate body weight, oocyte elimination and blood profile of rabbits infected with various doses of Eimeria stiedai isolates. The observed rabbits’ blood profile included erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, total protein plasma (TPP) and fibrinogen. Twenty-five male New Zealand White rabbits aged 3 months and weighed approximately 2 kg were provided with pellet and boiled drinking water and Eimeria stiedai isolates. The experiment used Completely Randomized Design to analyze 5 treatments with five replicates. The examined variables included D0: Infection 0 (control of infection without challenge test), D1: Infection 101 with challenge test 103, D2: infection 102 with challenge test 103, D3: infection 103 with challenge test 103, D4: infection 0 with challenge test 103 (control of infection). Data were subject to analysis of variance followed by Honestly Significant Difference Test (HSD). Analysis of Variance result showed that there was no significant difference on body weight, oocyte elimination and blood profile including erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, and fibrinogen. However, total protein plasma (TTP) was significantly different at 5% HSD. It can be concluded that challenge test with Eimeria stiedai has not been used as an alternative in increasing rabbits’ body immune against coccidiosis infection.
THE EFFICACIES OF BANANA STEM EXTRACT AS A CANDIDATE OF COCCIDIOSTAT AGAINST RABBIT EIMERIA STIEDAIO OCYSTS: AN IN VITRO ANALYSIS Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Wihadmadyatami, Hevi; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 17, No 3 (2015): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (741.083 KB) | DOI: 10.20884/1.anprod.2015.17.3.503

Abstract

The objective of this research was to investigatethe ability of banana stem (Musa paradisiaca) to inhibitsporulation of Eimeria stiedaioocystsderived fromrabbit by in vitroanalysis.Analyze the active substance proximate analysis and active substancesin this research were performed too. Banana stem extract were used in this experiment andsulfaquinoxalline(Coxy ®)was run as acontrol. The Eimeria stiedaioocystswere incubated prior the presence of  different concentration from banana stem extract  0%, 1%, 2%, 4%, 8%for 1, 2 and 3 daysat 26°C. In addition,Factorial patterned Completely Randomized Design (CRD) with five replicates wasapplied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulatedoocysts (P<0.01), unsporulatedoocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedaioocysts on in vitroexperiment. HSD test showed that the optimum potential efficacy of banana stem toinhibit sporulation was at 4% and 8% concentration during three days incubation.
TINGKAT INFEKSI DAN IDENTIFIKASI JENIS NEMATODA PENYEBAB NEMATODIASIS PADA SAPI POTONG BERBAGAI UMUR DI KECAMATAN KALIBAGOR KABUPATEN BANYUMAS Prawestry, Yuanita Adhelia; Indrasanti, Diana; Indradji, Mohandas
ANGON: Journal of Animal Science and Technology Vol 3 No 2 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1137.431 KB) | DOI: 10.20884/1.angon.2021.3.2.p201-213

Abstract

Background. The research aims to determine the rate of nematodiasis infection, identify the type of nematodes and know the relationship or influence of age on the number of occurrences of nematodiasis in beef cattle in Kalibagor Subdistrict, Banyumas Regency. The target of the study was beef cattle of various ages in Kalibagor Subdistrict, Banyumas Regency. Materials and Methods. The research method uses survey method with purposive sampling technique. The study used 96 samples, each sample as much as ± 3 grams of beef cattle. Data analysis used qualitative descriptive analysis and chi-square. Results. The sample test results showed as many as 72 positive samples infected with nematodiasis and 25 negative samples. Identification of nematode species obtained 7 types of nematodes that infect beef cattle, among others Trichuris sp., Strongyle sp., Cooperia sp., Bunostomum sp., Toxocara sp., Strongyloides sp., and Capillaria sp. The infection rate of nematodiasis in beef cattle in Kalibagor Subdistrict of Banyumas regency is high at 74.23%, with the infection rate in each child (1-8 months) at 21.65%, young (9-18 months) at 24.74%, and adults (>19 months) at 27.84%. Conclusion. The results of the data analysis using chi-square showed P > 0.05 means that there is no relationship between age and nematodiasis infection rate in cattle of various ages in Kalibagor Subdistrict, Banyumas Regency.
Rabbit’s Gastrointestinal Helminthiasis: Identification And Correlation with Age, Sex and Hygiene Indrasanti, Diana; Indradji, Mohandas; Yuwono, Endro; Ulfah, Annistia Rahmadian
ANIMAL PRODUCTION Vol. 27 No. 2 (2025)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2025.27.2.303

Abstract

The growing demand for high-protein low-fat meat has encouraged the development of potential rabbit farming. One of the obstacles in the rabbit farming is a disease. The studies of gastrointestinal helminthiasis in rabbits are limited, especially in Indonesia. The study aimed to identify and determine the prevalence of helmith infestation, and investigate the correlation between age, sex, and hygiene with the occurrence of gastrointestinal helminthiasis in small-scale rabbit farms. The survey was conducted on 476 rabbits in Banyumas District using a purposive sampling to collect fecal samples and questionnaire data. Both male and female rabbits were devided into four age groups: wean 1 (birth-1 month), wean 2 (>1-6 months), young (>6-18 months), and adult (>19 months). The feces were examined using the floating and the Whitlock methods and gastrointestinal helminthiasis were identified by observing the morphology of eggs and gastrointestinal helmiths. Data were subject to descriptive analysis and logistic regression using JASP software 0.16.3 version. The results demonstrated that 50 rabbits (10.50%) were infested with nematode and cestode worms. Nematode eggs found in rabbit feces in this study were strongyle (33.33%), Trichostrongylus (27.78%), Cittotaenia (14.81%), Passalurus (12.96%), Trichuris (7.41%), Hymenolepis (1.85%), Toxocara (1.85%) and Strongyloides (1.85%). The most prevalent egg worm in rabbits was Strongyle (33.33%) and lowest were Hymenolepis, Toxocara and Strongyloides (1.85% each). This study revealed that age and sex had no effect on the prevalence of gastrointestinal helminthiasis in rabbits (p>0.05). The frequency and technique of cage cleaning had a highly significant (p<0.01) effect on the occurence of gastrointestinal helminthiasis in rabbits, however there was no significant link with the separation of feces and urine in the cage (p>0.05).It can be inferred that the prevalence of gastrointestinal helminthiasis in rabbits is relatively low, and the management system including the hygiene of the cage are the most important factors in preventing its emergence.