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All Journal Animal Production : Indonesian Journal of Animal Production Journal of Applied Veterinary Science and Technology ANGON: Journal of Animal Science and Technology Animal Production
Mohandas Indradji
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Veterinary

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Body Weight, Oocyte Elimination and Blood Profile of Rabbit After Challenge Test Using Eimeria stiedai Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Fitriyani, Nur; Munawaroh, Sitie; Hikmawati, Nita; Irawan, Desika Indra
ANIMAL PRODUCTION Vol 20, No 1 (2018)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.469 KB) | DOI: 10.20884/1.jap.2018.20.1.621

Abstract

The objective of the research was to investigate body weight, oocyte elimination and blood profile of rabbits infected with various doses of Eimeria stiedai isolates. The observed rabbits’ blood profile included erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, total protein plasma (TPP) and fibrinogen. Twenty-five male New Zealand White rabbits aged 3 months and weighed approximately 2 kg were provided with pellet and boiled drinking water and Eimeria stiedai isolates. The experiment used Completely Randomized Design to analyze 5 treatments with five replicates. The examined variables included D0: Infection 0 (control of infection without challenge test), D1: Infection 101 with challenge test 103, D2: infection 102 with challenge test 103, D3: infection 103 with challenge test 103, D4: infection 0 with challenge test 103 (control of infection). Data were subject to analysis of variance followed by Honestly Significant Difference Test (HSD). Analysis of Variance result showed that there was no significant difference on body weight, oocyte elimination and blood profile including erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, and fibrinogen. However, total protein plasma (TTP) was significantly different at 5% HSD. It can be concluded that challenge test with Eimeria stiedai has not been used as an alternative in increasing rabbits’ body immune against coccidiosis infection.
THE EFFICACIES OF BANANA STEM EXTRACT AS A CANDIDATE OF COCCIDIOSTAT AGAINST RABBIT EIMERIA STIEDAIO OCYSTS: AN IN VITRO ANALYSIS Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Wihadmadyatami, Hevi; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 17, No 3 (2015): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (741.083 KB) | DOI: 10.20884/1.anprod.2015.17.3.503

Abstract

The objective of this research was to investigatethe ability of banana stem (Musa paradisiaca) to inhibitsporulation of Eimeria stiedaioocystsderived fromrabbit by in vitroanalysis.Analyze the active substance proximate analysis and active substancesin this research were performed too. Banana stem extract were used in this experiment andsulfaquinoxalline(Coxy ®)was run as acontrol. The Eimeria stiedaioocystswere incubated prior the presence of  different concentration from banana stem extract  0%, 1%, 2%, 4%, 8%for 1, 2 and 3 daysat 26°C. In addition,Factorial patterned Completely Randomized Design (CRD) with five replicates wasapplied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulatedoocysts (P<0.01), unsporulatedoocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedaioocysts on in vitroexperiment. HSD test showed that the optimum potential efficacy of banana stem toinhibit sporulation was at 4% and 8% concentration during three days incubation.
TINGKAT INFEKSI DAN IDENTIFIKASI JENIS NEMATODA PENYEBAB NEMATODIASIS PADA SAPI POTONG BERBAGAI UMUR DI KECAMATAN KALIBAGOR KABUPATEN BANYUMAS Prawestry, Yuanita Adhelia; Indrasanti, Diana; Indradji, Mohandas
ANGON: Journal of Animal Science and Technology Vol 3 No 2 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1137.431 KB) | DOI: 10.20884/1.angon.2021.3.2.p201-213

Abstract

Background. The research aims to determine the rate of nematodiasis infection, identify the type of nematodes and know the relationship or influence of age on the number of occurrences of nematodiasis in beef cattle in Kalibagor Subdistrict, Banyumas Regency. The target of the study was beef cattle of various ages in Kalibagor Subdistrict, Banyumas Regency. Materials and Methods. The research method uses survey method with purposive sampling technique. The study used 96 samples, each sample as much as ± 3 grams of beef cattle. Data analysis used qualitative descriptive analysis and chi-square. Results. The sample test results showed as many as 72 positive samples infected with nematodiasis and 25 negative samples. Identification of nematode species obtained 7 types of nematodes that infect beef cattle, among others Trichuris sp., Strongyle sp., Cooperia sp., Bunostomum sp., Toxocara sp., Strongyloides sp., and Capillaria sp. The infection rate of nematodiasis in beef cattle in Kalibagor Subdistrict of Banyumas regency is high at 74.23%, with the infection rate in each child (1-8 months) at 21.65%, young (9-18 months) at 24.74%, and adults (>19 months) at 27.84%. Conclusion. The results of the data analysis using chi-square showed P > 0.05 means that there is no relationship between age and nematodiasis infection rate in cattle of various ages in Kalibagor Subdistrict, Banyumas Regency.
Rabbit’s Gastrointestinal Helminthiasis: Identification And Correlation with Age, Sex and Hygiene Indrasanti, Diana; Indradji, Mohandas; Yuwono, Endro; Ulfah, Annistia Rahmadian
ANIMAL PRODUCTION Vol. 27 No. 2 (2025)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2025.27.2.303

Abstract

The growing demand for high-protein low-fat meat has encouraged the development of potential rabbit farming. One of the obstacles in the rabbit farming is a disease. The studies of gastrointestinal helminthiasis in rabbits are limited, especially in Indonesia. The study aimed to identify and determine the prevalence of helmith infestation, and investigate the correlation between age, sex, and hygiene with the occurrence of gastrointestinal helminthiasis in small-scale rabbit farms. The survey was conducted on 476 rabbits in Banyumas District using a purposive sampling to collect fecal samples and questionnaire data. Both male and female rabbits were devided into four age groups: wean 1 (birth-1 month), wean 2 (>1-6 months), young (>6-18 months), and adult (>19 months). The feces were examined using the floating and the Whitlock methods and gastrointestinal helminthiasis were identified by observing the morphology of eggs and gastrointestinal helmiths. Data were subject to descriptive analysis and logistic regression using JASP software 0.16.3 version. The results demonstrated that 50 rabbits (10.50%) were infested with nematode and cestode worms. Nematode eggs found in rabbit feces in this study were strongyle (33.33%), Trichostrongylus (27.78%), Cittotaenia (14.81%), Passalurus (12.96%), Trichuris (7.41%), Hymenolepis (1.85%), Toxocara (1.85%) and Strongyloides (1.85%). The most prevalent egg worm in rabbits was Strongyle (33.33%) and lowest were Hymenolepis, Toxocara and Strongyloides (1.85% each). This study revealed that age and sex had no effect on the prevalence of gastrointestinal helminthiasis in rabbits (p>0.05). The frequency and technique of cage cleaning had a highly significant (p<0.01) effect on the occurence of gastrointestinal helminthiasis in rabbits, however there was no significant link with the separation of feces and urine in the cage (p>0.05).It can be inferred that the prevalence of gastrointestinal helminthiasis in rabbits is relatively low, and the management system including the hygiene of the cage are the most important factors in preventing its emergence.
Penggunaan Acidifier sebagai Feed Additive dalam Pakan yang Mengandung Probiotik terhadap Bobot dan Prosentase Bursa Fabrisius dan Limpha Ayam Broiler Mahardi, Muhammad Koko; Hartoyo, Bambang; Indradji, Mohandas
ANGON: Journal of Animal Science and Technology Vol 2 No 3 (2020): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2020.2.3.p216-225

Abstract

TINGKAT INFEKSI DAN IDENTIFIKASI JENIS NEMATODA PENYEBAB NEMATODIASIS PADA SAPI POTONG BERBAGAI UMUR DI KECAMATAN KALIBAGOR KABUPATEN BANYUMAS Prawestry, Yuanita Adhelia; Indrasanti, Diana; Indradji, Mohandas
ANGON: Journal of Animal Science and Technology Vol 3 No 2 (2021): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2021.3.2.p201-213

Abstract

EFEKTIFITAS EKSTRAK HERBAL TERHADAP PROSES PENYEMBUHAN KELINCI YANG TERINFEKSI KOKSIDIOSIS Ulfah, Annistia Rahmadian; Indradji, Mohandas; Indrasanti, Diana; Ichwan, M. Nur; Rokayah, E
ANGON: Journal of Animal Science and Technology Vol 4 No 3 (2022): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2022.4.3.p344-349

Abstract

PENGARUH PEMBERIAN KOMBINASI TEMULAWAK (Curcuma zanthorrhiza) DAN BRATAWALI (Tinospora cordifolia) TERHADAP JUMLAH SEL DARAH MERAH, HEMOGLOBIN, DAN PACKED CELL VOLUME PADA AYAM BROILER Shintia, Friska Nur; Indradji, Mohandas; Indrasanti, Diana; Rahmadian Ulfah, Annistia
ANGON: Journal of Animal Science and Technology Vol 5 No 1 (2023): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2023.5.1.p106-116

Abstract

PENGARUH TINGKAT KEBERSIHAN TERHADAP JUMLAH BAKTERI COLIFORM DAN TELUR CACING PADA FESES AYAM BROILER PASCA PELARANGAN AGP DI KECAMATAN SUMBANG KABUPATEN BANYUMAS Ramadani, Muhammad Aditya; Indradji, Mohandas; Indrasanti, Diana
ANGON: Journal of Animal Science and Technology Vol 5 No 3 (2023): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2023.5.3.p347-357

Abstract

Latar Belakang. Tujuan dari penelitian ini adalah untuk mengetahui jumlah bakteri Coliform dan telur cacing serta pengaruh tingkat kebersihan terhadap jumlah bakteri Coliform dan telur cacing pada feses ayam broiler pasca pelarangan AGP di Kecamatan Sumbang Kabupaten Banyumas. Penelitian ini dilaksanakan pada tanggal 01 April 2022 sampai 02 Juni 2022 di peternakan ayam broiler Kecamatan Sumbang Kabupaten Banyumas. Materi dan Metode. Sasaran penelitian ini adalah ayam broiler fase starter dan finisher pada kandang tipe open house dan closed house di Kecamatan Sumbang Kabupaten Banyumas. Penentuan jumlah sampel untuk bakteri Coliform adalah 40 sampel dari 20 peternakan, untuk sampel telur cacing menggunakan rumus slovin dengan hasil 100 sampel. Penelitian ini menggunakan 25 gram untuk sampel bakteri Coliform dan 5 gram untuk sampel telur cacing. Variabel yang diamati adalah tingkat kebersihan terhadap jumlah bakteri Coliform dan tingkat kebersihan terhadap jumlah telur cacing. Metode untuk menghitung jumlah bakteri Coliform menggunakan Most Probable Number (MPN) dan menggunakan uji apung untuk menghitung jumlah telur cacing. Pengaruh tingkat kebersihan terhadap hasil pemeriksaan sampel dilakukan analisis Chi-square. Hasil. Hasil pemeriksaan sampel menunjukkan hasil jumlah sampel bakteri Coliform paling banyak terdapat pada kandang kategori sangat kotor, dengan hasil tingkat rendah sebanyak 14 atau 35%, dan pada tingkat sedang sebanyak 2 atau 5% dari total sampel, dan pada hasil jumlah telur cacing menunjukkan bahwa sampel negatif berjumlah 63% serta sampel yang positif berjumlah 37%, jumlah sampel positif juga menunjukkan bahwa ada 21 atau 56,7% dari total sampel positif yang menunjukkan infeksi ganda. Analisis data menggunakan Chi-square menunjukkan hasil P>0.05 yang berarti tidak ada hubungan signifikan antara tingkat kebersihan terhadap jumlah bakteri Coliform dan telur cacing pada feses ayam broiler pasca pelarangan AGP di Kecamatan Sumbang Kabupaten Banyumas. Simpulan. Kesimpulan yang didapatkan yaitu tingkat kebersihan tidak berpengaruh terhadap jumlah bakteri Coliform dan telur cacing, Jumlah bakteri Coliform paling banyak terdapat pada kategori kandang sangat kotor dengan tingkat rendah sebesar 35% dan tingkat sedang sebesar 5% dari total sampel, dan Jumlah telur cacing paling banyak terdapat pada kategori kandang kotor dengan tingkat rendah sebanyak 20% dan tingkat sedang sebanyak 9%.
Safety and Potential Test Profile of Inactivated Coryza Vaccine in SPF Chickens Saiful, Shilva Givanny; Indradji, Mohandas; Indrasanti, Diana; Andesfha, Ernes
Journal of Applied Veterinary Science And Technology Vol. 6 No. 2 (2025): October 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V6.I2.2025.96-101

Abstract

Background: Infectious coryza, caused by Avibacterium paragallinarum, is an acute and highly contagious respiratory disease in chickens that results in high morbidity, growth retardation, and decreased egg production, leading to economic losses in poultry industries. Vaccination is considered the most effective preventive measure, and inactivated vaccines are widely used due to their safety and ability to stimulate protective immunity. Purpose: This study aimed to evaluate the safety and potency of a commercially produced inactivated coryza vaccine using Specific Pathogen-Free (SPF) chickens at the National Quality Testing and Certification Center for Veterinary Drugs (BPMSPH), Indonesia. Method: A descriptive observational approach was used to assess safety and potency in vaccinated SPF chickens. A total of 40 SPF chickens four weeks old were used and divided into a vaccinated group and a control group. The vaccinated group received the inactivated coryza vaccine according to standard test procedures, while the control group remained unvaccinated. Clinical observations and local reaction assessments were conducted to evaluate safety, and antibody titers against coryza serotype A were measured to determine potency. Results: Observations showed that 100% of both control and vaccinated chickens exhibited no abnormal clinical signs or coryza reactions. In addition, the vaccine potency test showed that 100% of vaccinated chickens had coryza serotype A antibody titers ≥10. Conclusion: This test confirms that the registered inactivated coryza vaccine formulation meets safety test criteria: 100% of control and vaccinated chickens remained clinically normal, and no abnormal local reactions were observed at the inoculation site. Potency testing demonstrated that all vaccinated chickens developed serotype A antibodies, which are expected to provide protection against coryza infection in the field and improve poultry survival.
Co-Authors Andesfha, Ernes Bambang Hartoyo Dian Kurniawati Diana Indrasanti Endro Yuwono Hevi Wihadmadyatami Hikmawati, Nita Ichwan, M. Nur Irawan, Desika Indra Ismoyowati Izzat Munadiyan Mahardi, Muhammad Koko Munawaroh, Sitie Nasution, Ali Napiah Nur Fitriyani Pramono Soediarto Prawestry, Yuanita Adhelia Putri Meisari Rahmadian Ulfah, Annistia Ramadani, Muhammad Aditya Rokayah, E Saiful, Shilva Givanny Shintia, Friska Nur Siti Hilda A. Lestari Solly Aryza Ulfah, Annistia Rahmadian Utami Diah Pramesti