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All Journal Animal Production : Indonesian Journal of Animal Production ANGON: Journal of Animal Science and Technology
Pramono Soediarto
Fakultas Peternakan Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Veterinary

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Identification of Goats’ and Cows’ Milk Protein Profile in Banyumas Regency by Sodium Dedocyl Sulphate Gel Electrophoresis (Sds-Page) Widodo, Hermawan Setyo; Astuti, Triana Yuni; Soediarto, Pramono; Syamsi, Afduha Nurus
ANIMAL PRODUCTION Vol. 23 No. 1 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.1.37

Abstract

Protein is one of the nutrient components in milk that is related to product quality. The components of milk protein are divided into casein alpha-s1, beta, alpha-s2, kappa, and whey fractions such as alpha lactalbumin and beta lactoglobulin. There are no existing data of milk protein fraction in dairy cow and goats in Banyumas Regency. This study aimed to determine the profile in form of protein fractions of cow and goat milk in Banyumas. Milk sample from fifty cows and thirty dairy goats was taken by random sampling in some areas. The milk protein profile was identified by the technique of sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) and protein quantity prediction by software. The data obtained were analyzed statistically by Mann-Whitney between cows and goats. The results were significantly different (p<0.05) between cows and goats in molecular weight of protein alpha-S1 casein (29.66 vs 33.37 kDa), alpha-S2 (27.76 vs 29.49 kDa), beta (24 , 48 vs 25.59 kDa) and beta lactoglobulin (15.75 vs 15.97 kDa). The quantity of casein alpha-S1 (7.88 vs 4.16 g/l), alpha-S2 (1.31 vs. 4.02 g/l), beta (8.74 vs 14.24 g/l), kappa (2.41 vs. 4.28 g/l) and alpha lactalbumin (0.91 vs 0.7 g / l) was significantly different (p <0.05) between cow's and goat's milk, respectively. In conclusion, milk protein profile of cows and goats in Banyumas Regency is different.
Peran Penyuluh Terhadap Kualitas Susu Yang Dihasilkan Peternak Sapi Perah Ditinjau Dari Kadar Lemak Dan Berat Jenis Susu Di Kabupaten Wonosobo Wibowo, Muhammad Adi; Muatip, Krismiwati; Soediarto, Pramono
ANGON: Journal of Animal Science and Technology Vol 1 No 1 (2019): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2019.1.1.p1-11

Abstract

Background.The aim of the study was to determine the quality of milk produced by dairy farmers in Wonosobo Regency in terms of fat content and specific gravity, to know the role of extension agents so that dairy farmers produce quality dairy cows and analyze the relationship between the role of extension agents and milk quality produced by dairy farmers in terms of fat content and specific gravity. Materials and methodsThe research target is dairy farmers in Wonosobo Regency. Sampling of the area was carried out by purposive sampling, namely sub-districts that have a dairy cattle population in Wonososbo Regency. Census respondents took the research in selected regions, with 32 respondents as dairy farmers. Results. The analysis used is descriptive analysis and Rank Spearman analysis. Milk quality test results in the form of fat content and specific gravity of milk in Wonosobo District showed positive results, namely the average fat content above 2.9% and specific gravity above 1.0269 g/ml. The role of livestock extension agents in Wonosobo Regency has been good enough at helping dairy farmers to produce quality milk according to SNI standards. Conclusion. The Rank Spearman correlation analysis showed that there was a very significant relationship between the role of extension agents and the level of milk fat produced by farmers with a correlation coefficient of 0.525 and there was no relationship between the role of extension agents and milk quality seen from milk specific gravity with a correlation coefficient of 0.336.
PERSENTASE KERUSAKAN DAN TOTAL BAKTERI SUSU SEGAR DI KELOMPOK PETERNAK SAPI PERAH ANDINI LESTARI DAN TIRTA SARI AJI KABUPATEN BANYUMAS Abdurrahman, Isa Abdillah; subagyo, yusuf; Soediarto, Pramono
ANGON: Journal of Animal Science and Technology Vol 2 No 1 (2020): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2020.2.1.p29-38

Abstract

Background. The study was entitled "Percentage of Damage and Total Fresh Milk Bacteria in the Andini Lestari Dairy Farmer Group and Tirta Sari Aji Banyumas Regency". The purpose of this research was to determine percentage of damage and total bacteria in fress milk in the Andini Lestari dairy cattle group of Cilongok District and the Tirta Sari Aji dairy cattle in Karanglewas District. The research was conducted on 2 August 2019 until 28 September 2019. Materials and Methods. The material used is dairy farmers and milk. Dairy milk samples were obtained from 15 cattle from the Andini Lestari breeder group in Cilongok District and 15 cattle from Tirta Sari Aji, Karanglewas District, Banyumas Regency. The research method uses the survey method by conducting interviews and direct field observations. The response variables measured and observed in this study were milk damage and total milk bacteria. Milk samples obtained from the farms are then taken to the Dairy Production Laboratory for total microbial testing using the Total Plate Count Test and milk damage using the Alcohol Test. Results. The results showed that the alcohol test in the Andini Lestari group contained none positive samples or the presence of lumps in milk, while in the Tirta sari Aji group there were two positive samples of milk. Analysis of the sign test showed the level of milk damage in the two districts was not significantly different (P > 0.05). The results of total microbial studies in the two groups had an average of 16.22 x 104 cfu / ml and 14.44 x 104 cfu / ml. Analysis of the "t" test showed that the total microbes in the two groups were significantly different (P < 0.05). Conclusion. The quality of fresh milk in percentage of damage and total microbial in both Cilongok and Karanglewas Districts is considered good (according to SNI 2011).
Co-Authors Abdurrahman, Isa Abdillah Izzat Munadiyan Krismiwati Muatip Mohandas Indradji Nuhaeli Nuhaeli Nunung Noor Hidayat Puguh Arif Wibowo Syamsi, Afduha Nurus Triana Yuni Astuti Wibowo, Muhammad Adi Widodo, Hermawan Setyo Yusuf Subagyo