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Phytobiotic Properties of Garlic, Red Ginger, Turmeric and Kencur in Growing Ducks Ismoyowati, Ismoyowati; Indrasanti, Diana; Mufti, Mochamad; Farjam, Abdoreza Soleimani
ANIMAL PRODUCTION Vol 17, No 1 (2015): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Abstract. Phytobiotic properties of garlic (Allium sativum), turmeric (Curcuma domestica), red ginger (Zingiber officinale) and kencur (Kaempferia galangal) were studied using standard in vitro antibacterial test and in vivo feeding trial with ducklings. In the in vitro experiment, potency of aqueous extract of these phytobiotic agents were tested against Salmonella pullorum and Escherichia coli. Feeding trial was carried out for 6 week starting at day 28 using ducklings fed diets supplemented with 1% of each of four phytobiotic agents. The highest antibacterial activity against S. pullorum and E. coli was observed with garlic and no additive effect when mixture of phytobiotics was used. Weight gain, fed intake and feed conversion ratio of ducklings were not affected by inclusion of garlic, red ginger and kencur. However, 1% turmeric supplementation significantly reduced growth performance to ducklings.Â Key words: phytobiotic, antibiotic, duck, medicinal plantsÂ Abstrak.Â Penelitian karakteristik fitobiotik dari bawang putih (Allium sativum), kunyit (Curcuma domestica), jahe merah (Zingiber officinale) dan kencur (Kaempferia galangal) telah dilakukan secara in vitro melalui uji aktivitas antibakteri dan secara in vivo dengan perlakuan suplemetasi fitobiotik didalam pakan anak itik. Pada percobaanÂ in vitro, potensiÂ aktivitas antibakteri dari ektrak Â fitobiotik diuji menggunakan Salmonella pullorum and Escherichia coli.Â Percobaan suplementasi fitobiotik diberikan masing-masing sebesar 1% didalam pakan anak itik.Â Pemberian pakan perlakuan dilakukan selama 6 minggu,Â dimulai pada saat anak itik berumur 28 hari.Â Hasil penelitian menunjukkan aktivitas antibakteri terhadap S. pullorum dan E. coli paling tinggi adalah ektrak bawang putih dan tidak ada pengaruh yang lebih baik apabila dicampur dengan ektrak fitobiotik lainnya.Â Pertambahan bobot badan, konsumsi pakan dan konversi pakan anak itik tidak dipengaruhi oleh penambahan bawang putih, kunyit, jahe merah dan kencur.Â Akan tetapi, suplementasi kunyit nyata menurunkan performan pertumbuhan anak itik.Â Kata kunci: fitobiotik, antibiotik, itik, tanaman obat-obatan.

Nutrient Content of Napier Grass (Pennisetum purpureum) Silage Made with Various Additive and Modified Atmosphere in The Silo Widiyastuti, Titin; Hidayat, Nur; Indrasanti, Diana
ANIMAL PRODUCTION Vol 16, No 1 (2014): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.334 KB)

Abstract. During ensilage, anaerob condition must be controlled. Some methods of modified atmosphere in silo were analyzed to compare ensilage characteristics and silage product. So far, there is not been information on the atmosphere condition in the process of silage production. It encourages the researchers to evaluate the condition of ensilage process of Pennisetum purpureum by studying atmosphere modification in the silo and the effect of the usage of various additives in the process of silage production. Â Â Elephant grass (Pennisetum purpureum), molasses, L. acidophillus were used. The study was conducted with a Completely Randomized Design (CRD) 3x2 factorial pattern. Â Atmosphere modification as the first factor consist of : (A0: silage with compaction (conventional) A1: silage with vacuum method, A2: silage with modified C02) and two kinds of silage additives as the second factor (B1: indirect additive (molasses); B2: direct additive (Lactic Acid Bacteria).Â Each treatment combination was repeated 4 times. The objective of the research was to evaluate changes in nutrient content (protein, crude fiber, gross energy). The data obtained were analyzed by analysis of variance, then continued by Honest Significant Differences (HSD) test.Â Based on the research results it can be concluded that the optimum ensilage can take place, either by compaction methods (conventional), vacuum and the addition of CO2. While the addition of molasses additive produces silage with better quality than the addition of L. Acidophillus inoculant.Â Key words: Modified atmosphere, additives, silage Abstrak. Kondisi anaerob harus dikontrol selama ensilase. Beberapa metode modifikasi atmosfir silo dianalisa untuk membandingkan ciri dan hasil ensilase. Sejauh ini belum ada informasi kondisi atmosfir dalam proses produksi ensilase. Hal ini mendorong peneliti untuk mengevaluasi kondisi proses ensilase Pennisetum purpureum dengan mempelajari modifikasi atmosfir silo dan dampak penggunaan bermacam zat tambahan dalam proses pembuatan ensilase. Rumput gajah (Pennisetum purpureum), molase, L. acidophillus digunakan dalam penelitian dengan Rancangan Acak Lengkap (RAL)Â 3x2 polaÂ faktorial. Modifikasi atmosfir sebagai faktor pertama terdiri dari: (A0: silase dengan pemadatan (konvensional), A1: silase dengan metode vakum, A2: silase dengan modifikasi CO2) dan dua jenis silase tambahan sebagai faktor kedua: B1: tambahan langsung (molase); B2: tambahan langsung (Bakteri Asam Laktat). Setiap kombinasi perlakuan diulang 4 kali. Tujuan penelitian adalah mengevaluasi perubahan dalam kandungan nutrisi (protein, serat kasar, energi kasar). Data yang diperoleh dianalisis dengan analisis variansi, dilanjutkan uji Beda Nyata Jujur (BNJ). Kesimpulan penelitian adalah ensilase optimal tercapai baik dengan metode pemadatan (konvensional), vakum, maupun penambahan CO2. Sedangkan tambahan molase menghasilkan silase dengan kualitas yang lebih baik daripada dengan Â L. Acidophillus inoculant.Â Kata kunci: modifikasi atmosfir, zat tambahan, silase

Body Weight, Oocyte Elimination and Blood Profile of Rabbit After Challenge Test Using Eimeria stiedai Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Fitriyani, Nur; Munawaroh, Sitie; Hikmawati, Nita; Irawan, Desika Indra
ANIMAL PRODUCTION Vol 20, No 1 (2018)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

The objective of the research was to investigate body weight, oocyte elimination and blood profile of rabbits infected with various doses of Eimeria stiedai isolates. The observed rabbitsâ€™ blood profile included erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, total protein plasma (TPP) and fibrinogen. Twenty-five male New Zealand White rabbits aged 3 months and weighed approximately 2 kg were provided with pellet and boiled drinking water and Eimeria stiedai isolates. The experiment used Completely Randomized Design to analyze 5 treatments with five replicates. The examined variables included D0: Infection 0 (control of infection without challenge test), D1: Infection 101 with challenge test 103, D2: infection 102 with challenge test 103, D3: infection 103 with challenge test 103, D4: infection 0 with challenge test 103 (control of infection). Data were subject to analysis of variance followed by Honestly Significant Difference Test (HSD). Analysis of Variance result showed that there was no significant difference on body weight, oocyte elimination and blood profile including erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, and fibrinogen. However, total protein plasma (TTP) was significantly different at 5% HSD. It can be concluded that challenge test with Eimeria stiedai has not been used as an alternative in increasing rabbitsâ€™ body immune against coccidiosis infection.

THE EFFICACIES OF BANANA STEM EXTRACT AS A CANDIDATE OF COCCIDIOSTAT AGAINST RABBIT EIMERIA STIEDAIO OCYSTS: AN IN VITRO ANALYSIS Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Wihadmadyatami, Hevi; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 17, No 3 (2015): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

The objective of this research was to investigatethe ability of banana stem (Musa paradisiaca) to inhibitsporulation of Eimeria stiedaioocystsderived fromrabbit by in vitroanalysis.Analyze the active substance proximate analysis and active substancesin this research were performed too. Banana stem extract were used in this experiment andsulfaquinoxalline(Coxy ®)was run as acontrol. The Eimeria stiedaioocystswere incubated prior the presence of different concentration from banana stem extract 0%, 1%, 2%, 4%, 8%for 1, 2 and 3 daysat 26°C. In addition,Factorial patterned Completely Randomized Design (CRD) with five replicates wasapplied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulatedoocysts (P<0.01), unsporulatedoocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedaioocysts on in vitroexperiment. HSD test showed that the optimum potential efficacy of banana stem toinhibit sporulation was at 4% and 8% concentration during three days incubation.

Recent Study of Coccidiosis in Broiler Closed House: The Role of Some Aspects of Maintenance Yuwono, Endro; Wandatara, Reka Putra; Mastuti, Sri; Indrasanti, Diana
ANIMAL PRODUCTION Vol. 25 No. 2 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.2.207

This study aimed to determine the role of different factors in raising broiler chickens in closed-house cages that affect the increased cases of coccidiosis in partnership farms. These factors are cage management, the level of cage cleanliness, the chicken-rearing period, and the prevalence of coccidiosis in partnership farms. The research was conducted from May to December 2022 in 11 closed-house broiler farms in the Kalibagor Subdistrict, Banyumas Regency. This study applied survey method and a purposive sampling, and determined the sample size by the Slovin formula, collecting 100 samples in total. The materials were broiler chicken feces collected in the initial and final phases, and data on maintenance management were collected using a questionnaire. Data were subjected to descriptive analysis and Chi-square calculations. The results stated that the prevalence of coccidiosis in Kalibagor Subdistrict was 29%, and coccidiosis occurrence at the starter and finisher periods was 6% and 52%, respectively. The results of the Chi-square analysis show a significant difference in coccidiosis occurrence among broiler rearing periods. The effect of the level of cleanliness is also significant in the occurrence of coccidiosis in Kalibagor Subdistrict, Banyumas Regency. This study concludes that the occurrence of coccidiosis in closed-house broiler chickens on partnership farms is quite low, and the level of cleanliness and rearing period is very influential on the occurrence of coccidiosis.

Recent Study of Coccidiosis in Broiler Closed House: The Role of Some Aspects of Maintenance Yuwono, Endro; Wandatara, Reka Putra; Mastuti, Sri; Indrasanti, Diana
ANIMAL PRODUCTION Vol. 25 No. 2 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.2.207

This study aimed to determine the role of different factors in raising broiler chickens in closed-house cages that affect the increased cases of coccidiosis in partnership farms. These factors are cage management, the level of cage cleanliness, the chicken-rearing period, and the prevalence of coccidiosis in partnership farms. The research was conducted from May to December 2022 in 11 closed-house broiler farms in the Kalibagor Subdistrict, Banyumas Regency. This study applied survey method and a purposive sampling, and determined the sample size by the Slovin formula, collecting 100 samples in total. The materials were broiler chicken feces collected in the initial and final phases, and data on maintenance management were collected using a questionnaire. Data were subjected to descriptive analysis and Chi-square calculations. The results stated that the prevalence of coccidiosis in Kalibagor Subdistrict was 29%, and coccidiosis occurrence at the starter and finisher periods was 6% and 52%, respectively. The results of the Chi-square analysis show a significant difference in coccidiosis occurrence among broiler rearing periods. The effect of the level of cleanliness is also significant in the occurrence of coccidiosis in Kalibagor Subdistrict, Banyumas Regency. This study concludes that the occurrence of coccidiosis in closed-house broiler chickens on partnership farms is quite low, and the level of cleanliness and rearing period is very influential on the occurrence of coccidiosis.

TINGKAT INFEKSI DAN IDENTIFIKASI JENIS NEMATODA PENYEBAB NEMATODIASIS PADA SAPI POTONG BERBAGAI UMUR DI KECAMATAN KALIBAGOR KABUPATEN BANYUMAS Prawestry, Yuanita Adhelia; Indrasanti, Diana; Indradji, Mohandas
ANGON: Journal of Animal Science and Technology Vol 3 No 2 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Background. The research aims to determine the rate of nematodiasis infection, identify the type of nematodes and know the relationship or influence of age on the number of occurrences of nematodiasis in beef cattle in Kalibagor Subdistrict, Banyumas Regency. The target of the study was beef cattle of various ages in Kalibagor Subdistrict, Banyumas Regency. Materials and Methods. The research method uses survey method with purposive sampling technique. The study used 96 samples, each sample as much as ± 3 grams of beef cattle. Data analysis used qualitative descriptive analysis and chi-square. Results. The sample test results showed as many as 72 positive samples infected with nematodiasis and 25 negative samples. Identification of nematode species obtained 7 types of nematodes that infect beef cattle, among others Trichuris sp., Strongyle sp., Cooperia sp., Bunostomum sp., Toxocara sp., Strongyloides sp., and Capillaria sp. The infection rate of nematodiasis in beef cattle in Kalibagor Subdistrict of Banyumas regency is high at 74.23%, with the infection rate in each child (1-8 months) at 21.65%, young (9-18 months) at 24.74%, and adults (>19 months) at 27.84%. Conclusion. The results of the data analysis using chi-square showed P > 0.05 means that there is no relationship between age and nematodiasis infection rate in cattle of various ages in Kalibagor Subdistrict, Banyumas Regency.

PREVALENSI DAN PENGARUH KEBERSIHAN KANDANG TERHADAP KEJADIAN NEMATODIASIS PADA TERNAK KAMBING DI KECAMATAN SUMBANG KABUPATEN BANYUMAS Ayuwandari, Eka Aditya; Indrasanti, Diana; Yuwono, Endro
ANGON: Journal of Animal Science and Technology Vol 6 No 2 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2024.6.2.p161-168

Penelitian bertujuan untuk mengetahui jenis nematoda, tingkat prevalensi, dan pengaruh kebersihan kandang terhadap kejadian nematodiasis pada ternak kambing di Kecamatan Sumbang Kabupaten Banyumas. Metode penelitian yang digunakan yaitu metode survei. Sampel ditentukan dengan rumus purposive sampling. Sampel berupa feses dari ternak kambing sebanyak 99 ekor. Feses yang diambil berasal dari kambing jantan dan betina dengan kategori umur cempe (umur 3-5 bulan), muda (umur 6-12 bulan), dan dewasa (>12 bulan). Variabel yang diamati yaitu jenis nematoda, prevalensi nematodiasis pada berbagai kategori kebersihan kandang, dan pengaruh kebersihan kandang terhadap kejadian nematodiasis. Pengujian feses yang digunakan yaitu uji apung dan Whitlock. Jenis nematoda, prevalensi nematodiasis dianalisis dengan analisis deskriptif dan analisis Chi-Square. Prevalensi nematodiasis di Kecamatan Sumbang memiliki peresentase 71,71%. Jenis telur cacing nematoda yang menginfeksi ternak kambing yaitu Ostertagia sp, Cooperia sp, Oesophagostomum sp, Bunostomum sp, Trichostrongylus sp, Strongyles sp, Strangyloides sp, Haemonchus sp, Capillaria sp, Trichuris sp. Hasil analisis Chi-Square menunjukkan tidak terdapat pengaruh antara tingkat kebersihan kandang terhadap nematodiasis (P>0,05). Kesimpulan dari penelitian ini adalah tingkat kebersihan kandang tidak berpengaruh secara signifikan terhadap kejadian nematodiasis.

Rabbit’s Gastrointestinal Helminthiasis: Identification And Correlation with Age, Sex and Hygiene Indrasanti, Diana; Indradji, Mohandas; Yuwono, Endro; Ulfah, Annistia Rahmadian
ANIMAL PRODUCTION Vol. 27 No. 2 (2025)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2025.27.2.303

The growing demand for high-protein low-fat meat has encouraged the development of potential rabbit farming. One of the obstacles in the rabbit farming is a disease. The studies of gastrointestinal helminthiasis in rabbits are limited, especially in Indonesia. The study aimed to identify and determine the prevalence of helmith infestation, and investigate the correlation between age, sex, and hygiene with the occurrence of gastrointestinal helminthiasis in small-scale rabbit farms. The survey was conducted on 476 rabbits in Banyumas District using a purposive sampling to collect fecal samples and questionnaire data. Both male and female rabbits were devided into four age groups: wean 1 (birth-1 month), wean 2 (>1-6 months), young (>6-18 months), and adult (>19 months). The feces were examined using the floating and the Whitlock methods and gastrointestinal helminthiasis were identified by observing the morphology of eggs and gastrointestinal helmiths. Data were subject to descriptive analysis and logistic regression using JASP software 0.16.3 version. The results demonstrated that 50 rabbits (10.50%) were infested with nematode and cestode worms. Nematode eggs found in rabbit feces in this study were strongyle (33.33%), Trichostrongylus (27.78%), Cittotaenia (14.81%), Passalurus (12.96%), Trichuris (7.41%), Hymenolepis (1.85%), Toxocara (1.85%) and Strongyloides (1.85%). The most prevalent egg worm in rabbits was Strongyle (33.33%) and lowest were Hymenolepis, Toxocara and Strongyloides (1.85% each). This study revealed that age and sex had no effect on the prevalence of gastrointestinal helminthiasis in rabbits (p>0.05). The frequency and technique of cage cleaning had a highly significant (p<0.01) effect on the occurence of gastrointestinal helminthiasis in rabbits, however there was no significant link with the separation of feces and urine in the cage (p>0.05).It can be inferred that the prevalence of gastrointestinal helminthiasis in rabbits is relatively low, and the management system including the hygiene of the cage are the most important factors in preventing its emergence.

Prevalensi Koksidiosis dan Identifikasi Eimeria sp. Pada Kelinci Berbagai Umur di Kecamatan Kalibagor Kabupaten Banyumas Pramudya, Ruzicca Arif; Sufiriyanto, Sufiriyanto; Indrasanti, Diana
ANGON: Journal of Animal Science and Technology Vol 2 No 2 (2020): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2020.2.2.p156-166

Background. The purpose of this study are to determine the prevalence of coccidiosis, to know the species of Eimeria, and to find out the difference in the prevalence of coccidiosis in various age rabbits in Kalibagor Subdistrict Banyumas Regency. Materials and methods. The targets of this study is various types of rabbits in Kalibagor Subdistrict Banyumas Regency at three periods, namely weaning (1-5 weeks), growing (6-24 weeks), and adult (>24 weeks) with a minimum 3 rabbit of each farmer. The method used was a survey method using purposive sampling. Data analysis was performed using descriptive and chi square methods. The samples used for this study were rabbit's faeces as much as 5 grams per animal in the weaning period as many as 31 , 31 growing, and 31 adult. Result. The results of the analysis show that the species of Eimeria identified as infecting rabbits includes Eimeria magna, Eimeria perforans, Eimeria flavescens, Eimeria intestinalis, Eimeria exigua, Eimeria media, Eimeria coecicola, and Eimeria stiedae. The prevalence of coccidiosis in rabbits weaning period was 29,03%, the growing was 31,18%, and the adult was 29,03% and the total prevalence of coccidiosis were 89,24%. P>0,05 shows that there are no difference in the prevalence of coccidiosis in rabbits of various ages in Kalibagor Subdistrict Banyumas Regency. Conclusion. The conlusion of this study is that the prevalence of coccidiosis was noy significantly different in rabbits in weaning, growing, and adult period.

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