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Identifikasi Immunohistokimiawi Desmin dan Vimentin dalam Sel Otot Skelet Ayam Kedu Cemani (Galllus gallus domesticus) Budipitojo, Teguh; Wihadmadyatami, Hevi; -, Ariana; Musana, Dewi K.
Journal of Biota Biota Volume 19 Nomor 1 Tahun 2014
Publisher : PBI Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.065 KB)

AbstractThe two sub units of intermediate filaments, desmin and vimentin, are found in most cells of mesenchymal origin. The content and distribution of desmin in mature skeletal muscle have been known, but for vimentin has been a subject of disagreement. The present study was aimed to clarify the presence of desmin and vimentin immunoreactivities in the skeletal muscle fibers of Kedu Cemani chickens (Gallus gallus domesticus) by immunohistochemistry methods of avidin-biotin-complexs. Samples of muscle tissues were obtained from pectorales mayor, biceps brachii, and biceps femoris of five adult Kedu Cemani chickens (Gallus gallus domesticus). Immunohistochemical staining results showed that desmin immunoreactivities were detected in Z disc of myofibers, but not vimentin. The results stimulate further exploration on characteristics of Kedu Cemani chickens, especially in terms of the distribution of other intermediate filaments and neuroendocrine cells in a variety of organs system.Key words: Kedu chicken, skeletal muscle, desmin, vimentin, immunohistochemistryAbstrakVimentin dan desmin merupakan subunit filamen intermedia dan dapat ditemukan dalam sebagian besar sel mesenkimal. Keberadaan dan distribusi desmin dalam sel otot skelet dewasa/masak telah diketahui, namun keberadaan dan distribusi vimentin pada sel yang sama masih menjadi perdebatan. Penelitian ini bertujuan mengklarifikasi keberadaan immunoreaktifitas desmin dan vimentin dalam serabut otot skelet ayam kedu cemani dengan metode immunohistokimia komplek avidin-biotin. Penelitian dilakukan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani (Gallus gallus domesticus). Pengamatan terhadap immunoreaktifitas desmin dan vimentin dalam serabut otot normal ayam kedu cemani dewasa dengan menggunakan antibodi monoklonal terhadap desmin dan antibodi poliklonal terhadap vimentin memperoleh hasil bahwa immunoreaktifitas desmin terdeteksi pada diskus Z serabut otot skelet, namun immunoreaktifitas vimentin tidak terdeteksi. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani, terutama dalam hal distribusi filamen intermedia dan jenis neuroendokrin lainnya pada berbagai sistem organ tubuh.Kata kunci: Ayam kedu, otot skelet, desmin, vimentin, immunohistokimia

Identifikasi Immunohistokimiawi Desmin dan Vimentin dalam Sel Otot Skelet Ayam Kedu Cemani (Galllus gallus domesticus) Budipitojo, Teguh; Wihadmadyatami, Hevi; -, Ariana; Musana, Dewi K.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 19, No 1 (2014): February 2014
Publisher : Universitas Atma Jaya Yogyakarta

AbstractThe two sub units of intermediate filaments, desmin and vimentin, are found in most cells of mesenchymal origin. The content and distribution of desmin in mature skeletal muscle have been known, but for vimentin has been a subject of disagreement. The present study was aimed to clarify the presence of desmin and vimentin immunoreactivities in the skeletal muscle fibers of Kedu Cemani chickens (Gallus gallus domesticus) by immunohistochemistry methods of avidin-biotin-complexs. Samples of muscle tissues were obtained from pectorales mayor, biceps brachii, and biceps femoris of five adult Kedu Cemani chickens (Gallus gallus domesticus). Immunohistochemical staining results showed that desmin immunoreactivities were detected in Z disc of myofibers, but not vimentin. The results stimulate further exploration on characteristics of Kedu Cemani chickens, especially in terms of the distribution of other intermediate filaments and neuroendocrine cells in a variety of organs system.Key words: Kedu chicken, skeletal muscle, desmin, vimentin, immunohistochemistryAbstrakVimentin dan desmin merupakan subunit filamen intermedia dan dapat ditemukan dalam sebagian besar sel mesenkimal. Keberadaan dan distribusi desmin dalam sel otot skelet dewasa/masak telah diketahui, namun keberadaan dan distribusi vimentin pada sel yang sama masih menjadi perdebatan. Penelitian ini bertujuan mengklarifikasi keberadaan immunoreaktifitas desmin dan vimentin dalam serabut otot skelet ayam kedu cemani dengan metode immunohistokimia komplek avidin-biotin. Penelitian dilakukan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani (Gallus gallus domesticus). Pengamatan terhadap immunoreaktifitas desmin dan vimentin dalam serabut otot normal ayam kedu cemani dewasa dengan menggunakan antibodi monoklonal terhadap desmin dan antibodi poliklonal terhadap vimentin memperoleh hasil bahwa immunoreaktifitas desmin terdeteksi pada diskus Z serabut otot skelet, namun immunoreaktifitas vimentin tidak terdeteksi. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani, terutama dalam hal distribusi filamen intermedia dan jenis neuroendokrin lainnya pada berbagai sistem organ tubuh.Kata kunci: Ayam kedu, otot skelet, desmin, vimentin, immunohistokimia

THE EFFICACIES OF BANANA STEM EXTRACT AS A CANDIDATE OF COCCIDIOSTAT AGAINST RABBIT EIMERIA STIEDAIO OCYSTS: AN IN VITRO ANALYSIS Indrasanti, Diana; Indradji, Mohandas; Hastuti, Sri; Wihadmadyatami, Hevi; Ismoyowati, Ismoyowati
ANIMAL PRODUCTION Vol 17, No 3 (2015): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

The objective of this research was to investigatethe ability of banana stem (Musa paradisiaca) to inhibitsporulation of Eimeria stiedaioocystsderived fromrabbit by in vitroanalysis.Analyze the active substance proximate analysis and active substancesin this research were performed too. Banana stem extract were used in this experiment andsulfaquinoxalline(Coxy ®)was run as acontrol. The Eimeria stiedaioocystswere incubated prior the presence of different concentration from banana stem extract 0%, 1%, 2%, 4%, 8%for 1, 2 and 3 daysat 26°C. In addition,Factorial patterned Completely Randomized Design (CRD) with five replicates wasapplied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulatedoocysts (P<0.01), unsporulatedoocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedaioocysts on in vitroexperiment. HSD test showed that the optimum potential efficacy of banana stem toinhibit sporulation was at 4% and 8% concentration during three days incubation.

Isolasi dan identifikasi ekspresi protein rekombinan granule-1 (GRA-1) Toxoplasma gondii isolat lokal Hevi Wihadmadyatami; Rini Widayanti; Wayan Tunas Artama
Jurnal Sain Veteriner Vol 29, No 2 (2011): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Toxoplasmosis is a diseases caused by intracellular protozoa called Toxoplasma gondii.

Identifikasi Sifat dan Distribusi Sel Endokrin Ghrelin di Lambung Tikus (Rattus norvegicus): Studi Immunohis-Tokimia pada Kondisi Obesitas Teguh Budipitojo; Hevi Wihadmadyatami; Ganies Riza Aristya
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 1 (2016): June
Publisher : Universitas Gadjah Mada

Obesity is one of major nutritional problems in the world. Obesity is very dangerous, especially when concentrated in the abdomen, because it is closely linked to various diseases such as diabetes, hypertension, heart disease, which can to causing death. This study aims to identify the nature and distribution of ghrelin on gastric endocrine cells in the obese rat (Rattus norvegicus) by using immunohistochemical techniques. The results will strengthen the understanding of the role and function of ghrelin as an alternative therapeutic target on obesity. The research used gastric tissues of ten obese and control rats which were stained with avidin-biotin-peroxidase complex method of immunohistochemistry. The results showed the existence of two types of ghrelin-producing cells (open and closed types) on the gastric mucosa of control rats, and only one type of ghrelin producing cells (open type) in obese rats. The intensity of ghrelin immunoreactive positive cells was detected weak in obese rats, but very strong in control rats. Ghrelin endocrine cells mainly distributed in the basal part of the gastric mucosa of the fundus parts, with a very small number in obese rats, but highly abundant in control rats. This study confirmed the decrease of the ghrelin synthesis and secretion in obese rat (Rattus norvegicus) at the cellular level. The decrease of ghrelin synthesis is characterized by a reduction on the number of ghrelin producing cells, the disappearance of the close type of ghrelin producing cells, and the low activity of protein synthesis in the ghrelin producing cells. Ghrelin endocrine cells distributed mainly in the basal part of the gastric mucosa, especially in the fundus parts.

Morphological Structure of the Tongue of Gekko gecko in Yogyakarta, Indonesia Aqiilah, Khairunnisa; Budipitojo, Teguh; Wijayanto, Hery; Budiariati, Vista; Pangestiningsih, Tri Wahyu; Ariana, Ariana; Saragih, Golda Rani; Kustiati, Ulayatul; Wihadmadyatami, Hevi
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 4 (2024): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.90995

Gekko gecko is a member of the order Squamata from the family Gekkonidae and mainly feeds on small insects. This study aims to determine the morphology of the tongue of Gekko gecko through Scanning Electron Microscopy (SEM), hematoxylin–eosin (HE) staining, and immunohistochemistry (IHC). Six adult Gekko gecko were obtained from the Special Region of Yogyakarta, and Gekko gecko tongue samples were stored in SEM fixative solution and then observed with SEM. For histochemical and IHC staining, tongue samples were processed into paraffin blocks and cut into 8 µm-thick sections. The SEM revealed three types of papillae: dome-shaped papillae at the apex, fan-shaped papillae at the corpus, and scale–like papillae at the radix. Histological observations showed that the tongue of the Gekko gecko was composed of tunica mucosa and tunica muscularis, and goblet cells were present in the lamina of the epithelial mucosa. Meanwhile, no taste buds were found. Immunoreactivity against PGP 9.5 was observed on the tunica muscularis of the apex, corpus, and radix. Taken together, this study provides new insight into the tongue morphology of Gekko gecko and is dominated by mechanical papillae on the tongue surface.

Comparative Scanning Electron Microscopy Study on Scale Variations in Indonesian Cultivated Koi Fish (Cyprinus rubrofuscus Lacepede, 1803) Andrian, Krisna Noli; Wihadmadyatami, Hevi; Wijayanti, Nastiti; Karnati, Srikanth; Haryanto, Aris
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.242-252

Koi (Cyprinus rubrofuscus Lacepede, 1803) is a highly favored ornamental fish due to its beauty and wide range of variations in Indonesia, categorized by color, patterns, and scales. Some variants are distinguished by color, while others, such as Ginrin, Doitsu, and Shusui, have unique scale types. Despite visible differences, microscopic scale variations remain unexplored. SEM studies in other fish species offer insights into scale ultrastructure, providing opportunities for comparison. Therefore, this study aimed to uncover the microscopic structure of four types of koi fish, namely Doitsu, Ginrin, Shusui, and common scale. Koi fish were obtained from breeders in Yogyakarta, Indonesia, and acclimatized in an aquarium. Scale extraction was conducted under anesthesia using MS-222 and the cleaned scales were then subjected to dehydration, fixation, and affixed to double-sided adhesive tape for SEM analysis. Furthermore, coating with conductive gold enabled observation using SEM at 10 kV, allowing examination of scale features such as focus, radii, circuli, and lepidonts at various magnifications. The results showed that based on SEM analysis, significant differences were observed in scale structures among koi variants. At low magnification, differences in tubercles and lepidonts were observed, particularly between common and Ginrin scale types. Shusui scale showed unique characteristics with a closer arrangement of circuli and distinctive lepidont shapes. At higher magnification, clearer details of radii, circuli, and lepidonts were observed, further highlighting the differences among koi variants. SEM provides crucial insights into the morphology of scales in koi fish variants, showing unseen macroscopic differences and distinct features such as tubercles and lepidont frequency.

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