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All Journal Journal of Educational, Health and Community Psychology MPI (Media Pharmaceutica Indonesiana) Jurnal Purifikasi Pharmaceutical Journal of Indonesia Hubungan Tingkat Pengetahuan Petugas Pengelola Obat dengan Tingkat Ketersediaan Obat Di Puskesmas Kota Malang Berkala Penelitian Hayati Makara Journal of Science Jurnal Farmasi Indonesia
Indrajati Kohar
Laboratorium Kimia Farmasi, Fakultas Farmasi, Universitas Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Rapid screening analysis of antioxidant activities in green tea products using DPPH and FRAP Setiawan, Vendra; Phangestu, Sherlly; Soetikno, Agatha Grace; Arianti, Angelina; Kohar, Indrajati
Pharmaceutical Journal of Indonesia Vol. 7 No. 1 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.pji.2021.007.01.2

Abstract

Free radicals are formed inside and outside the body. Free radicals inside the body can be created in the cell compartments. The reactions are complex through the initiation, propagation, and termination stages. On the other hand, free radicals in the outer body can be formed from pollutions and environmental damage action.  Excessive free radicals cause oxidative stress and use destructive cellular to affect health conditions. Antioxidants are known as essential agents in biological systems against diseases triggered by free radicals. One of the many antioxidants found in tea plants (Camellia sinensis) is polyphenols. A colorimetry method was employed to evaluate the dominant antioxidant compound of the total phenolic and flavonoid content in two selected tea products. The total phenolic content was determined by utilizing the Folin-Ciocalteu reagent. In addition, an aluminum colorimetric assay is applied to assess flavonoid content. Rapid screening of antioxidant activity using a spectrophotometric method is employed to determine IC50 values of tea extracts utilizing DPPH and FRAP. The FRAP method showed more sensitivity in determining antioxidant activities with the feasibility of working in low analyte concentrations than the DPPH method. The high total phenolic and flavonoid contents found in this experiment are in line with low IC50 values. It was also found out that brewed green tea has lower total phenol content than green tea bags.
Co-Authors Agustanti, Onie Allaf, Karim Anggara, Aris Sri Arianti, Angelina Deby Vinolia Diana Indriany Imelda Inge Lika2 Jonatan, Melyana Leon Janssen Lika, Imelda Inge Mario Mario Melyana Jonatan Nanik S. Wilujeng Ni Nyoman Tri Puspaningsih Onie Agustanti Phangestu, Sherlly Poppy Hartatie Hardjo Poppy Hartatie Hardjo, Poppy Hartatie Poppy Hartatie Hardjo2 poppy_hardjo@ubaya.ac.id, Poppy Hartatie Rahayu, Yusti Probowati Rizky Pradita Manafe Ryanto Budiono Setiawan, Vendra Soediatmoko Soediman Soetikno, Agatha Grace Yunita, Oeke