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Journal : Buletin Peternakan

Identification of Conjugated Linoleic Acid in Milk Fermented by Probiotics Originating in the Gastrointestinal Tract Kafaah Estancia; Widodo Widodo
Buletin Peternakan Vol 43, No 2 (2019): BULETIN PETERNAKAN VOL. 43 (2) MAY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i2.17325

Abstract

One of the benefits of probiotics is their ability to synthesize conjugated linoleic acid (CLA) as a functional bioactive compound. The objective of this study is to identify CLA synthesized in milk fermented with the probiotic Lactobacillus casei strain AP and Lactobacillus casei strain AG as starter cultures. Fermented milk products were analyzed using gas chromatography-mass spectrometry (GC-MS) to characterize the CLA formed. The result of GC-MS in milk fermented using Lactobacillus casei strain AG was detection of a CLA compound with a retention time of 41.467, whereas in milk fermented using Lactobacillus casei strain AP, linoleic acid, but not CLA, was detected.
ACTIVITY OF PHENOL OF Morinda citrifolia AS NATURAL ANTIBACTERIA TO INHIBIT THE GROWTH OF MASTITIS-ASSOCIATED BACTERIA Theresia Ika Purwantiningsih; Yuni Suranindyah; Widodo Widodo
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i4.24159

Abstract

Most of dairy farmers less of attention about sanitation after milking. It can trigger a disease, mastitis. Mastitis is one of harmful disease in dairy cow, because it can decrease quality and quantity of milk. The aim of this study was to determine active compounds in the noni fruit extract, to measure the levels of phenolic compounds in different stage of fruit maturity and to know the result of California Mastitis Test (CMT) test between teat’s cow were dipped with noni fruit extract and commercial teat dipping solution containing 1.25% iodine (commercial). The highest level of phenols was presented in ripe noni fruit. Based on statistical analysis using One Way Anova followed by Duncan's test Multiply Range Test (DMRT), there were significant differences (P<0.05) in CMT test between teat’s cow were dipped in noni fruit extract and iodine 1.25% solution. Noni fruit extract solution showed better result than commercial dipping solution containing iodine. The conclusion is noni fruit extract contains natural antibacteria, it can be used to teat dipping solution.
Effects of Four Essential Oils on Nutrients Digestibility of In Vitro Ruminal Fermentation Asih Kurniawati; Widodo Widodo; Wayan Tunas Artama; Lies Mira Yusiati
Buletin Peternakan Vol 42, No 2 (2018): BULETIN PETERNAKAN VOL. 42 (2) MAY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i2.33522

Abstract

This research was done to study the effect of four essential oils (EOs), as feed additives, on ruminal nutrient digestibility in order to find out candidate of rumen modifier. Those four EOs were destilated from Foeniculum vulgare (Mill), Pinus merkusii (Jungh. & de Vriese), Cymbopogon nardus (L.) Rendle and Melaleuca leucadendra (L.). Their effect on dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF) digestibility were studied using in vitro rumen fermentation technique according to Theodorou method. Data were statistically analysed using analysis of variance factorial 4x5 design. Feed for fermentation substrate consist of Pennisetum purpureum, rice bran and wheat pollard. Essential oil individually was added and mix with substrate to meet the final concentration in fermentation media of 0, 100, 200, 400 and 800 mg/l. P. merkusii, C. nardus and M. leucadendra EOs reduced DM, OM, and CF digestibilities. The decreasing were in line with the increasing of EOs doses. Therefore F. vulgare only reduced CF digestibility. The value of DM, OM and CP digestibilities in fermentation with F. vulgare did not differ from control. CP digestibility differed among EO treatments. In F. vulgare, and P. merkusii, treatments, CP digestibility did not differ from control, whereas overall CP digestibility in C. nardus tended to be higher than control and CP digestibility in M. leucadendra was significantly higher. All EOs addition reduced CF digestibility at all level. CF digestibility in fermentation added 800 mg/l of M. leucadendra EO was slumped to only 14.29% of control. Among four EOs M. leucadendra is the most potent on interrupt rumen feed fermentation whereas P. merkusii and C. nardus in moderate ways and F. vulgare in delicate manner.