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Effect of SOYXYL as a Dietary Protected Protein Supplement on the Reproductive Performance of Simmental Bulls Prasetiyono, Bambang Waluyo Hadi Eko; Widiyanto, Widiyanto; Ondho, Yon Soepri; Subrata, Agung; Widodo, Hermawan Setyo
ANIMAL PRODUCTION Vol. 22 No. 1 (2020)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2020.22.1.41

Abstract

The study aimed to evaluate the effect of SOYXYL as one of the dietary protected protein supplement product in the rations on the reproduction performance of Simmental bulls. Twelve 5 years-old Simmental bulls were allotted to 4 treatment groups with 3 replications in each, i.e.: T0= control ration (elephant grass + 5 kg/head/day commercial concentrate), T1= control ration + 150 g protected protein supplement SOYXYL (3% of concentrate), T2 = control ration + 300 g SOYXYL (6% of concentrate) and T3= control ration + 450 g SOYXYL (9% of concentrate). The experiment lasted for 90 days. At the end of the experiment, semen and blood were collected and body weight gain was measured. The variables measured included sperm motility, sperm concentration, blood protein, blood urea and the concentration of testosterone. Results showed that SOYXYL supplementation, particularly at the levels of 3 and 6%, increased the total plasma protein of bulls. SOYXYL supplementation at 3% from concentrate resulted in increased plasma urea concentration. Feeding SOYXYL, particularly at the levels of 6 and 9%, increased the average daily gain of bulls as compared to control. Feeding 3% SOYXYL increased, but at the levels of 6 and 9% decreased the concentration of sperm of bulls. The concentration of testosterone was higher in the bulls received 3 and 6% SOYXYL in the rations. Dietary supplementation of SOYXYL increased the sperm motility of Simmental bulls. In conclusion, dietary supplementation of SOYXYL at the level of 3% increased plasma protein, testosterone, and sperm concentrations as well as sperm motility. However, supplementation of SOYXYL at 6 and 9% from rations decreased the concentrations of sperm and testosterone.
Synchronization Protein-Energy Index of Various Forages for Dairy Livestock: an In Vitro Study Syamsi, Afduha Nurus; Widodo, Hermawan Setyo
ANIMAL PRODUCTION Vol. 22 No. 2 (2020)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (861.105 KB) | DOI: 10.20884/1.jap.2020.22.2.34

Abstract

The present study inventoried the protein-energy synchronization (PES) index of various types of grass. The study was conducted using in vitro techniques with material 5 types of grass (elephant, king, dwarf elephant, guinea, and setaria) and rumen fluid of the Friesian Holstein (FH). The PES index is calculated based on the average degradation of g protein and Kg organic matter (OM) per hour at the time of observation of 2nd, 4th, 6th, 8th, 12th, 24th, 48th, and 72nd hours. The hourly degradation rates were analyzed by linear regression to obtain the average hourly degradation rate. The results were included in the PES index calculation formula and discussed descriptively. This study found that all five grass had almost uniform nutritional value. Regression analysis results show a different equation, but with a high coefficient of determination (> 90%) on all types of grass. The PES index obtained in elephant, king, dwarf elephant, guinea, and setaria grass are 0.72, 0.66, 0.69, 0.58, and 0.68. The study concluded that elephant grass has a PES index at a high level, while king, dwarf elephant, guinea, and setaria grass at medium level for compiling dairy rations.
Identification of Goats’ and Cows’ Milk Protein Profile in Banyumas Regency by Sodium Dedocyl Sulphate Gel Electrophoresis (Sds-Page) Widodo, Hermawan Setyo; Astuti, Triana Yuni; Soediarto, Pramono; Syamsi, Afduha Nurus
ANIMAL PRODUCTION Vol. 23 No. 1 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with Animal Scientist Society of Indonesia (ISPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.1.37

Abstract

Protein is one of the nutrient components in milk that is related to product quality. The components of milk protein are divided into casein alpha-s1, beta, alpha-s2, kappa, and whey fractions such as alpha lactalbumin and beta lactoglobulin. There are no existing data of milk protein fraction in dairy cow and goats in Banyumas Regency. This study aimed to determine the profile in form of protein fractions of cow and goat milk in Banyumas. Milk sample from fifty cows and thirty dairy goats was taken by random sampling in some areas. The milk protein profile was identified by the technique of sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) and protein quantity prediction by software. The data obtained were analyzed statistically by Mann-Whitney between cows and goats. The results were significantly different (p<0.05) between cows and goats in molecular weight of protein alpha-S1 casein (29.66 vs 33.37 kDa), alpha-S2 (27.76 vs 29.49 kDa), beta (24 , 48 vs 25.59 kDa) and beta lactoglobulin (15.75 vs 15.97 kDa). The quantity of casein alpha-S1 (7.88 vs 4.16 g/l), alpha-S2 (1.31 vs. 4.02 g/l), beta (8.74 vs 14.24 g/l), kappa (2.41 vs. 4.28 g/l) and alpha lactalbumin (0.91 vs 0.7 g / l) was significantly different (p <0.05) between cow's and goat's milk, respectively. In conclusion, milk protein profile of cows and goats in Banyumas Regency is different.
Kajian keamanan pangan dan tingkat prevalensi cemaran bakterisusu di Sentra Pengembangan Sapi Perah Cilongok Afduha Nurus Syamsi; Triana Yuni Astuti; Hermawan Setyo Widodo
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 28, No 3 (2018): Desember
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2018.028.03.05

Abstract

The experiment was aimed to assess the food securitythrough physical properties, chemical composition and prevalence rate of milk bacterial contamination in Cilongok dairy cattle development center. Surveys and observations were conducted to obtain data on milking and milk processing applied by farmer and to obtain data on physical properties, chemical composition, and milk total bacterial count. The materialswere used farmer and milk samples taken 20 liters in total from 13 Andini Lestari 1 farmers and 7 Andini Lestrari 2 farmers. Data from the laboratory were analyzed descriptively based on the Standard Nasional Indonesia (SNI): 3141.1.2011, "t" test was used to distinguish milk quality based on physical properties, chemical composition and total bactery from both groups. The results showed that physical properties, chemical composition, and total bactery from both groups were not significantly different (P> 0.05). The conclusion of the research stated that Cilongok Dairy Cattle Development Center produces milk that is suitable for consumption based on physical and chemical composition in accordance with SNI standard: No.3141.1.2011 that showed low prevalence of bacterial contamination.
Mengidentifikasi Peptida Bioaktif Angiotensin Converting Enzyme-inhibitor (ACEi) dari Kasein β Susu Kambing dengan Polimorfismenya Melalui Teknik In Silico Hermawan Setyo Widodo; Tridjoko Wisnu Murti; Ali Agus; Widodo Widodo
Jurnal Aplikasi Teknologi Pangan Vol 7, No 4 (2018): November 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.952 KB) | DOI: 10.17728/jatp.3008

Abstract

Susu kambing memiliki komponen protein salah satunya protein β dan secara umum terjadi polimorfisme pada level protein. Perubahan urutan asam amino akibat polimorfisme memungkinkan adanya potensi dihasilkannya peptida bioaktif penghambat enzim pengubah angiotensin (ACEi). Penelitian ini bertujuan untuk menyaring peptida bioaktif yang berpotensi sebagai ACEi dari kasein β kambing beserta polimorfismenya. Penelitian ini dilakukan dengan teknik in silico terhadap sekuen kasein β kambing serta struktur tiga dimensi human testicular ACE. Langkah yang dilakukan dalam penelitian ini meliputi simulasi pemotongan peptida dengan enzim pencernaan (pepsin, tripsin dan kimotripsin), peninjauan karakteristik peptida lalu simulasi docking ligan-reseptor. Tampilan parameter Lipinski’s Rule of Five (Ro5), bioaktivitas dan energi afinitas dipertimbangkan untuk memilih peptida bioaktif. Hasil yang didapat menunjukkan bahwa ditemukan peptida bioaktif yakni INK (Ile-Asp-Lys) yang memiliki kemampuan hampir setara dengan lisinopril (afinitas energi -8,2kkal/mol vs. -8,3kkal/mol). Peptida INK dapat ditemukan dari hasil hidrolisis dari alel A, C, D dan E, sehingga polimorfisme tidak menyebabkan perbedaan produksi peptida bioaktif. Kesimpulan yang dapat diambil yakni kasein β susu kambing jika dicerna dengan enzim pencernaan dapat menghasilkan peptida bioaktif ACEi yakni INK.Identification of Angiotensin Converting Enzyme-inhibitor (ACEi) Bioactive Peptide from Goat Milk β-Casein with It's Polymorphism by In Silico TechniqueAbstractPolymorphism eventually may be occurred at the protein level. Changes in the amino acid sequence due to polymorphism may exhibit a potential action to generate of the angiotensin-converting enzyme inhibitors (ACEi) bioactive peptide. This study is aimed to assess bioactive peptides that have a great potent value as ACEi from goat β casein along with its polymorphism. The research was done by in silico technique on goat β-casein sequence and three-dimensional structure human testicular ACE. Peptide-cutting simulations with digestive enzymes (pepsin, trypsin and chymotrypsin), peptide properties review, then ligand-receptor docking simulations was applied in this research. Appearance of Lipinski's Rule of Five (Ro5), bioactivity and affinity energy were considered for selecting bioactive peptides. The results show that bioactive peptide found as INK (Ile-Asp-Lys) which had similar ability as lisinopril (energy affinity –8.2kcal/mol vs. –8.3kcal/mol). The INK peptides could be found from the hydrolysis resulted in alleles A, C, D and E, therefore polymorphism did not affect the differences of production of bioactive peptides. A conclusion, processed goat milk β casein with digestive enzymes could produce ACEi of INK as bioactive peptide.
Pengenalan Alternatif Usaha Bagi Santri Pondok Pesantren Salaf Al Anwar Bogangin Melalui Pelatihan Teknologi Pengolahan Hasil Ternak Afduha Nurus Syamsi; Lis Safitri; Hermawan Setyo Widodo; Dewi Puspita Candrasari
Jurnal Pengabdian Masyarakat Indonesia Vol 1 No 3 (2021): JPMI - Juni 2021
Publisher : CV Infinite Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52436/1.jpmi.19

Abstract

Pondok Pesantren Al Anwar Bogangin merupakan pondok pesantren salaf yang masih menerapkan model pembelajaran tradisional. Santri tidak mendapatkan pendidikan formal, sehingga perlu diberikan pelatihan keterampilan. Tujuan kegiatan ini adalah untuk memberikan pelatihan keterampilan teknologi pengolahan hasil ternak sebagai alternatif usaha santri dimasa mendatang. Metode yang digunakan yaitu sosialisasi, penyuluhan dan pelatihan, serta praktek, diakhiri dengan evaluasi. Hasil kegiatan menunjukkan bahwa santri sebagai kelompok sasaran mampu membuat produk yang serupa dengan model percontohan yang diberikan tim penyuluh. Hasil evaluasi menunjukkan bahwa kegiatan pelatihan mampu meningkatkan pengetahuan dan keterampilan peserta dengan hasil peningkatan nilai sebesar 57.43% pada kelompok santri putra dan peningkatan sebesar 60% pada santri putri pada nilai post test dibandingkan nilai pre test. Sebanyak 100% peserta menyatakan kegiatan menarik dan 68% menyatakan berminat untuk membuat usaha dengan bahan serupa materi pelatihan. Berdasarkan hasil kegiatan dapat disimpulkan bahwa pengenalan teknologi pengolahan hasil ternak sebagai alternatif usaha Santri Pondok Pesantern Salaf Al Anwar berhasil dilaksanakan dengan menarik, meningkatkan pengetahuan dan keterampilan, serta menimbulkan minat sebagian besar santri untuk berwirausaha pada bidang serupa.
Macronutrient Digestibilities and Enzyme Activities in Rumen Fluid Supplemented by Protein-Energy Synchronized Index-Based Rations Afduha Nurus Syamsi; Harwanto Harwanto; Hermawan Setyo Widodo; Yusuf Subagyo
Buletin Peternakan Vol 46, No 2 (2022): BULETIN PETERNAKAN VOL. 46 (2) MAY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i2.73881

Abstract

The protein-energy  synchronized  (PES)  index-based  rations  can  optimize microbial  protein  synthesis  (MPS)   and   affect   enzyme   activity   and  macronutrient digestibility of ration in rumen fluid. This study aimed to examine the in vitro effect of a ration based on PES index on macronutrient digestibility and enzyme activity in rumen fluid. The research was conducted experimentally, consisting of 4 treatment rations with different  PES  indexes  (R1:  0.55;  R2:  0.6;  R3:  0.65;  R4:  0.7).  Each  treatment  was repeated 5 times, so there were 24 experimental units. The material used was the rumen fluid of the Jawa Randu Goat, which was taken at the Sokaraja Slaughterhouse shortly after  the  goat  was  slaughtered.  Each  ration consisted  of  napier  grass,  river  tamarind, coconut  meal, soybean  dregs,  rice  bran,  cassava  waste,  and  mineral  mix.  The  results showed  that  the  PES  index  significantly  affected  (P<0,01)  the  digestibility  of  protein (A),  fiber  (B),  and  fat  (C),  as  well  as  the  activity  of  protease  (D)  and  cellulase  (E) enzymes.  Theorthogonal  polynomial  test  showed  that  the  PES  index  has  a  quadratic effect on all test parameters with the following equation: A (Y= -1229.5X2 + 1540.6X -457.57; R2= 0.75), B (Y = -800.95X2+ 955.86X -264.51; R² = 0.75), C (Y = 868.92X2-1038.2X + 325.1; R2= 0.81), D (Y = -41.4X2+ 51.697X -14.982; R² = 0.77), and E (Y = -4.8538X2+  5.927X -1.6241;  R²  =  0.84).  The  protein-energy  synchronized  index-based rations increased the in vitro of macronutrient digestibility (protein, fiber, and fat) and enzyme activity on rumen fluid at a medium index level (0.6-0.63).
KORELASI ANTARA BOBOT BADAN DENGAN LINGKAR DADA KAMBING KEJOBONG DI KTT NGUDI DADI PURBALINGGA Dewi Puspita Candrasari; Agus Susanto; Setya Agus Santosa; Dattadewi Purwantini; Hermawan Setyo Widodo; Chomsiatun Nurul Hidayah
ANGON: Journal of Animal Science and Technology Vol 4 No 3 (2022): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (753.003 KB) | DOI: 10.20884/1.angon.2022.4.3.p297-302

Abstract

Background. Local goats are widely cultivated in Indonesia, especially in Kejobong, Purbalingga. Local farmers usually determine the price of livestock from body weight. To make it easier, it can be done by measuring the chest circumference, which is quite accurate compared to the results of the scales. The purpose of the study was to estimate the body weight (BW) of Kejobong goats using chest circumference (LD) measurements. The purpose of this study was to estimate the body weight (BB) of Kejobong goats using chest circumference (LD). The materials used were 38 female Kejobong goats aged 3-12 months and 51 heads >12-30 months. Samples were taken using purposive random sampling. The variables observed were the BB and LD of female Kejobong goats. The calculation results show a correlation (r) in the age group 3-12 months of 0.984, in the age group >12-30 months the correlation is 0.691 and in all age groups of 0.942. The results of the correlation between LD and BB obtained a linear regression equation for female Kejobong goats aged 3-12 months Y= -21.433+0.6552x, in the age group >12-48 months Y= -21.916+0.735x and in all age groups Y= -28.802+0.812x. The linear regression equation can be used to estimate the body weight of female Kejobong goats at all ages.
KAJIAN METODE TITRASI FORMOL DALAM PENGUKURAN PROTEIN SUSU SEGAR MELALUI VALIDASI METODE ANALISIS (VMA) Hermawan Setyo Widodo; Jodi Susanto; Yusuf Subagyo; Afduha Nurus Syamsi; Merryafinola Ifani
ANGON: Journal of Animal Science and Technology Vol 4 No 3 (2022): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (807.28 KB) | DOI: 10.20884/1.angon.2022.4.3.p303-309

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Background. This research was conducted to determine the feasibility of the formol titration method in measuring protein content in fresh milk. Material and method. The material used in this study was a sample of fresh cow's milk. Standard milk was prepared by adding hydrolyzed casein to obtain protein content of 10%, 8%, 6%, 4% and 2%. Each standard was triplicate tested to obtain LOD, LOQ, accuracy and precision calculations. The data obtained is then presented descriptively. Results. The results obtained show that the formol titration method achieves an LOD value of 2.12%, LOQ of 6.41%, an accuracy rate of 145.56% and a precision of 2;4;6;8;10% are 3.58;4.40;3.12;3.58;2.58%. The resulting linear curve has a regression equation Y=2.0228X – 2.785 with an R2 value of 0.957. Conclusion. It can be concluded that the measurement of milk protein using the formol titration method is quite good, but less accurate because it tends to be overquantify.
ANALISIS PERAMALAN PRODUKSI SUSU BERBAGAI TERNAK PERAH DI INDONESIA MENGGUNAKAN METODE AUTOREGRESSIVE INTEGRATED MOVING AVERAGE (ARIMA) Danang Nur Cahyo; Krismiwati Muatip; Afduha Nurus Syamsi; Hermawan Setyo Widodo; Merryafinola Ifani
ANGON: Journal of Animal Science and Technology Vol 4 No 3 (2022): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1009.709 KB) | DOI: 10.20884/1.angon.2022.4.3.p310-321

Abstract

metabolized by the human body. There are various dairy livestock spread around Indonesia specifically dairy cattle, dairy goats, buffalo, and sheep. This research aimed to forecast the milk production of various dairy livestock in Indonesia until 2028. The research method of this study was the secondary data method utilizing secondary data from the Food and Agriculture Organization (FAO) of the United Nation. Data used in this study was milk production of Indonesia from various dairy livestock in tonnes from 2012-2021. The Autoregressive integrated moving average (ARIMA) method was used to forecast milk production in Indonesia. The (p,d,q) value for the milk production forecast of dairy cattle, dairy goat, buffalo, and sheep were (1,1,1), (2,1,1), (0,0,1), and (1,1,1) respectively. Milk production for cows, goats and sheep is predicted to continue to increase until 2028, while buffalo milk production is predicted to decrease. Milk production from various dairy livestock besides dairy cattle has great potential to develop so as to create diversity in fulfilling the needs of dairy products in Indonesia.