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All Journal Indonesian Journal of Biotechnology Jurnal Ilmu Kefarmasian Indonesia
Umar Anggara Jenie
Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Impact of Curcuma mangga Val. Rhizome Essential Oil to p53, Bcl-2, H-Ras and Caspase-9 expression of Myeloma Cell Line Endang Astuti; Retno Sunarminingsih; Umar Anggara Jenie; Sofia Mubarika; S. Sismindari
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.993 KB) | DOI: 10.22146/ijbiotech.8631

Abstract

Cancer is a disease, a public health problem, which is found in the world as well as in Indonesia. Ingeneral, some of cancer theraphies are ineffective, characterized by the resistance performance of cancer cell line,the exposed normal cell and by the side effects. Nowadays, studies to fi nd the specifi c and safely anti-cancerdrugs were increased by the time. Several studies revealed that Curcuma mangga Val. Rhizome contains somesecondary metabolites, essential or non-essential oil, which has cytotoxic activities to the cancer cells. Basedon these anti-cancer potentials, this study has several aims to recognize anti-cancer selectivity and molecularmechanism by inducting apoptosis and inhibiting myeloma cell proliferation. To C. mangga Val. essential oil,immunocyto chemical test was performed to determine the expression of p53, caspase-9, Bcl-2, H-Ras proteinwhile TUNEL test was performed to determine the number of apoptosis cells.The results of this study shown that anti-cancer molecular mechanism of C. mangga Val. essential oil tomyeloma cell line was performed by increasing apoptosis; by increasing the expression of pro-apoptosis p53,caspase-9 protein and reducing protein which is increasing proliferation Bcl-2 and H-Ras.
Konstruksi dan Validasi Protokol Skrining Virtual Berbasis Struktur dengan Kode PDB 3MQE, 3NTG, dan 3LN0 untuk Penemuan Inhibitor Siklooksigenase-2 (COX-2) ESTI MUMPUNI; ARGUN WIDARSA; YANTI SUSILAWATI; OISAN OISAN; ARIEF NURROCHMAD; HARNO DWI PRANOWO; UMAR ANGGARA JENIE; ENADE PERDANA ISTYASTONO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 1 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Cyclooxygenase-2 (COX-2) inhibitors are high demand drugs in the market. However, available COX-2 inhibitors nowadays have many side effects. Therefore, there is still a need to develop more potent selective COX-2 inhibitors and one of the method that has been prove the effectivity and eficiency for new drugs research is in silico. Structure-based virtual screening (SBVS) protocols were developed to find COX-2 inhibitors using the Protein-Ligand ANT System (PLANTS) docking software, SPORES, BKChem and Open Babel. The directory of useful decoys (DUD) dataset for COX-2 was used to validate the protocols retrospectively; the DUD consist of 426 known COX-2 inhibitors and 13289 decoys. Based on criteria value of EF20% and EFmax used in the article from Huang et al (2006) and Yuniarti et al (2011), two validated protocol, AYO_COX2_v.1.1 and AYO_COX2_v.1.2 , showed good results
Pengaruh Perlakuan PGV-1, PGV-0 dan Kurkumin terhadap Protein yang Terlibat dalam Siklus Sel Fase G2-M dan Apoptosis pada Sel Kanker Payudara T 47D MUHAMMAD DA'I; UMAR ANGGARA JENIE; SUPARDJAN AM; EDY MEIYANTO; MASASHI KAWAICHI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 10 No 2 (2012): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Previous experiments showed that curcumin analogue (PGV-1) inhibited breast cancer cell (T47D) growth at G2-M phase and induced cell apoptosis. This experiment was conducted to investigate the molecular effect of another curcumin analogue, PGV-0 and curcumin on the cell cycle progression and apoptosis as compared to the PGV-1. F lowcytometric method was conducted to analyze the effect of PGV-1 (2,5 µM), PGV-0 (5,0 µM) and curcumin (10,0 µM) on the cell distribution of various phase of T47D cell cycle. Western blot was also conducted to observe the effect of those compounds on proteins that involved in cell cycle (i.e. p21 and Cdc-Z) and apoptosis (Caspase-3/7/9). The results showed that PGV-1, PGV-0 and curcumin induced hyperploidy phenomenon on T47D cell. PGV-1 inhibited the cell cycle specifically on G2-M phase. Molecular observation showed that PGV-1 and PGV-0 were able to increase the expression of p21 protein and the Cdc-2 proteins, whilst curcumin was able to activate the Cdc-2 protein. The compounds have ability to induce apoptosis on T47D cell via Caspase3/7 activation. In conclusion, PGV-1, PGV-0 and curcumin inhibited the T47D cell cycle progression and induced cell apoptosis.
Synthesis and Structure Elucidation of 1,3 bis(p-Hydroxyphenyl)urea HARI PURNOMO; UMAR ANGGARA JENIE; AGUNG ENDRO NUGROHO; HARNO DWI PRANOWO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 1 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Paracetamol is a well known and commonly used analgesic-antipyretical agent. However, it exhibits hepatotoxic side effect if used for the long term or using exessive dose (10-15 g for single dose). A new compound of 1.3 bis (p-hydroxyphenyl)urea (code-name as HP2009) is an analgesic agent, and it is predicted that its hepatotoxic side effect is lower than that of paracetamol. The compound HP2009 was succesfully synthesized. The result showed that by using molar ratios of p-aminophenol and urea 2:9.5, pH 1 for reaction condition, refluxing for about 1 hr and evaporating time was set up for 30 minutes, the yield of HP 2009 will be 99.49%. The crystals obtained was characterized using spectroscopic methods, and showed undoubtedly that the product was 1.3 bis(p-hydroxyphenyl)urea.
Virtual Screening and Bonding Mode Elucidation of Curcumin Analogue in Cyclooxygenase-2 Enzyme Using EE_COX2_V.1.0 Protocol ESTI MUMPUNI; ARIEF NURROCHMAD; UMAR ANGGARA JENIE; HARNO DWI PRANOWO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 2 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Kurkumin adalah senyawa fenol bewarna kuning yang terkandung dalam Curcuma longa. Kurkumin diketahui memiliki aktivitas biologi antara lain sebagai inhibitor beberapa enzim metabolisme. Modifikasi struktur kurkumin telah banyak dilakukan. Pada penelitian ini dilakukan penapisan virtual dan elusidasi moda ikatan analog kurkumin menggunakan EE_COX2_V.1.0 sebagai protokol skrining virtual berbasis struktur yang telah tervalidasi. Pengaturan simulasi docking dilakukan menggunakan berbagai aplikasi yang terintegrasi seperti SPORES, PLANTS, BKchem, OpenBabel dan Pymol yang dapat mengidentifikasi senyawa inhibitor siklooksigenase-2 (COX-2). Dari hasil penapisan virtual didapatkan senyawa demetoksikurkumin dengan 3 residu asam amino dan 1,7-bis(3-metoksifenil)-1,6-heptadien-3,5-dion dengan 6 residu asam amino aktif in silico sebagai inhibitor COX-2.
Co-Authors Agung Endro Nugroho ARGUN WIDARSA ARIEF NURROCHMAD EDY MEIYANTO Enade Perdana Istyastono Endang Astuti Esti Mumpuni ESTI MUMPUNI HARI PURNOMO Harno Dwi Pranowo MASASHI KAWAICHI Muhammad Da’i OISAN OISAN Retno Sunarminingsih Sudibyo S. Sismindari Sofia Mubarika Haryana SUPARDJAN AM YANTI SUSILAWATI